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The anti-inflammatory activity of five copper complexes is shown in the cotton wad granuloma test in Rats. The activity due to copper seems to be only modulated by the ligands in the complexes studied.

We describe a sensitive liquid phase radioimmunoassay for serum IgD. Extreme values obtained from 85 control patients sera are 0.2 and 121 mg/l with an arithmetic mean of 25 mg/l. In atopic patients (with high serum IgE levels), arithmetic mean is 47 mg/l.

Ovine prolactin (o-PRL) binding to rat mammary gland membranes has been shown to vary during the estrous cycle in the normal rat. In this study we report the characteristics of o-PRL binding to mammary gland membranes throughout the estrous cycle in a new rat strain, IPL nude, which presents a total absence of lactation. Prolactin receptors were quantified in the 100 000 g pellet. The mean value of the affinity constant in IPL nude rat was 16.7 X 10(9) M-1 and no variation was observed throughout the estrous cycle. The binding capacity also remained unchanged and the values were situated between 11.6 +/- 1.1. fmoles/mg protein and 38.6 +/- 12.3 fmoles/mg protein, corresponding to the lowest values obtained in normal rat. On the day of estrus, the prolactin binding capacity in the mammary gland of the IPL nude rat was significantly different from that of normal rat. These findings confirm that prolactin certainly plays an important role in the induction or stimulation of the rat mammary gland function.

(N-2-amino-2-deoxy-alpha, beta-D-glucopyranose salicylaldimino)-Cu (II) shows an anti-inflammatory activity in the cotton wad granuloma on Rats. An increase of the copper level in serum is detected very soon after s.c. drug administration, which is due to the high diffusion of the complex.

The authors studied experimentally ovario-uterine implantation in rats, an operation first performed many years ago yielding very rare and debatable results. This procedure is ineffective in the treatment of sterility due to endometrial proliferation over the ovary, which closes off rapidly the endometrial cavity and separates it from the implanted ovary. Much uncertainty surrounds the majority of previously reported successes, the few resultant pregnancies from ovario-uterine implantation considered due to hazard and completely unreliable. This operation has no place in the treatment of sterility.

A substance presenting a beta-casein-like immunoreactivity (beta-cas-LI) was found in male serum and tissues. This beta-cas-LI was measured by a specific radioimmunoassay using a purified beta-casein and an anti beta-casein kindly supplied by L. M. Houdebine. Serial dilutions of serum or tissues samples showed a good parallelism to the standard curve. In male rat, beta-cas-Li was found in all sera and in some tissues = mammary gland, prostate and testis which contained respectively 6,200, 408 and 38 ng per organ. beta-cas-LI value in the serum was regulatable. Hypophysectomy and long-term castration strongly reduced basal levels. Insertion of testosterone silastic capsules (25 mg/capsule) was able to restore control values, while estradiol (E2) (4 mg/kg E2 valerianate) to castrated or intact rats increased serum beta-cas-LI above basal values. In conclusion, these results showed, for the first time, the presence in male rat serum and tissues of a beta-casein immunoreactive-like substance. Moreover, this beta-cas-LI seemed to be under pituitary and gonad regulation.