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To determine the possible protective and enhancer role of selenium foliar application on oil yield, fatty acid composition and glucosinolate content of rapeseed cultivars under late-season thermal stress, a factorial split-plot experiment based on randomized complete block design with three replications was carried out in Karaj, Iran during the two growing seasons 2014–2015 and 2015–2016. Three sowing dates (Oct. 7 as normal planting date), (Oct. 17 as semi-late planting date), (Oct. 27 as late planting date) and two selenium foliar application (non-application as control and sodium selenate as foliar application) were factorially randomized to main plots, and rapeseed cultivars were allocated to sub-plots. Plant materials were six cultivars including three hybrids and three Iranian open pollinated varieties. Late-season thermal stress caused by late planting date reduced oil yield, oil content, oleic acid, linoleic acid, palmitic acid and increased linolenic acid, erucic acid and glucosinolate content of investigated cultivars. The results of this study demonstrated that the highest amount of oil yield, oil content, oleic acid, linoleic acid, palmitic acid and the lowest amount of erucic acid and glucosinolate content were observed in L72 cultivar in both control and selenium application treatments. This study provided new important findings about the supportive and enhancer role of selenium in the form of sodium selenate on quantity and quality of oil in rapeseed plant.