Search

Escherichia coli (E. coli) is a bacterial bacillus known to be negative for the gram dye, and it is part of the normal flora inside the human body. The current study aimed to investigate the possibility of using lipopolysaccharides (LPS) as an antimicrobial agent. A total of 30 isolates of E. coli were collected from 100 specimens of urine isolated from patients with recurrent urinary tract infections (UTIs) referring to Al-Sadr Teaching Hospital. The samples were cultured on identification media; thereafter, they were diagnosed according to the phenotypic form, biochemical tests, and finally by VITK-2. The results of the prevalence of E. coli isolates illustrated that from 30 isolates of E. coli which were collected from 100 urine specimens, 14 (46.6%), 6 (20%), and 10 (33.3%) cases belonged to the urinary tract infections, kidney stones, and urinary catheter samples, respectively. The isolates displayed multiple drug resistance (MDR) to most of the antibiotics used in the study. Therefore, the lipopolysaccharide extracted from E. coli was used as an antimicrobial agent. The recorded data obtained from the polymerase chain reaction (PCR) for WaaA, WaaC, wamB, and wabG genes demonstrated that the isolates possessed biosynthesis genes for LPS. The results indicated that LPS at concentrations of 150, 250, and 500 μg/ml has as an anti-growth agent for Klebsiella pneumonia, Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Bacillus subtilis, Staph aureus, and candida albicans. The highest inhibitory effect was observed on K. pneumonia, P. mirabilis, S. typhi, B. subtilis, and C. albicans, as compared to the control antimicrobials agents (Amoxicillin, Nystatin). The LPS loaded on liposome nanoparticles can open new horizons in medicine by its inclusion in the manufacture of broad-spectrum antibiotics.

Burkholderia cepacia is found as part of the B. cepacia complex (Bcc), a collection of highly pathogenic organisms. The Bcc is present almost everywhere in nature; however, it is most prevalent in damp settings, plant roots, and soils. Moreover, Bcc is a major source of morbidity and death in patients due to its high intrinsic antibiotic resistance. The present study aims to isolate and identify gram-negative aerobic bacteria from clinical samples derived from a variety of pathological diseases and investigate the bacterium's virulence factors and genes. The current study included 250 specimens collected from patients suffering from diabetic foot ulcers, urine, burn, wound, sputum, and discharge from the eyes. The samples were collected from both sexes with the age range of 1-75 years. The recorded data showed that males had a higher frequency of infection (79.2%) than females (52%). The results revealed that 7.6% of infected females were between 1-15 years old, while 22% of infected males were aged between 31-45 years. In addition, 26.8% of infected patients (both males and females) were aged between 31-45 years.

Wireless Sensor Networks have highly proved its contribution in precision agriculture. Communication channel modelling investigation is a highly demanded to achieve a successful communication system where a good wave propagation model is crucially needed. Precision agriculture degrades the traveling waves in various forms in addition to the effects of the large-scale path losses models. This paper reviews the most known theoretical large-scale path losses models such as free space (FSPL) and Plane earth (PE) models as well as the vegetation models represented by Weissberger, ITU-R, FITU-R and COS235 models. Indeed, this work illustrates the effects of many factors on the total path losses such as separation distance between transceivers, antenna heights and the depth of vegetation that presence in the path propagation. In conclusion, the total path loss is computed based on large-scale path losses and the vegetation losses in protected vegetation environment.

Interleukin-17A (IL-17A) is a member of the Interleukin-17 family, which belongs to the pro-inflammatory cystine-knot cytokines. Recent studies on the etiology of breast cancer have focused on the role of immunity and inflammation. The pro-inflammatory cytokines IL-17A can medicate cancer-related inflammation. The present study aimed to analyze the mutation in physicochemical properties and structure of the Interleukin-17Agenein developing breast cancer using bioinformatics methods. A total of 60 blood samples were obtained from Iraqi women aged 25 to 75 with breast cancer. Twenty blood samples were obtained from healthy women in the same age range as a control group. Deletion and missense mutations detected by BLAST in samples with breast cancer. The present study determined the physicochemical properties of IL-17A such as hydrophilic nature, alpha-helical and 3D structure. The results of this study indicated that IL-17Ais considered a marker for a patient with breast cancer. Also, mutations in the IL-17Agene affect the structure and physicochemical properties of the IL-17A protein complex.

Little consideration has been given to how case study might be used in poststructural research to explore power relations that constitute a phenomenon. Many case study scholars, most notably Robert Yin, adopt a postpositivist perspective that assumes the "truth" can be accessed through applying prescriptive and rigid research techniques. Using a discussion of Michel Foucault's key theoretical ideas and the insights gained through a Foucauldian case study of people with advanced cancer who continue to receive curative treatment, the authors argue for the expansion of case study in poststructural inquiry. They propose that the use of poststructuralist case study is valuable because of the flexibility and comprehensiveness of the methodology, which allows for the exploration of a deeper understanding of the broader discourses that shape a phenomenon, as well as how power/knowledge relations shape the behaviours and perceptions of people. They also introduce the reflexive implications of poststructural case study research., (Copyright© by Ingram School of Nursing, McGill University.)