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In-depth analysis of the influence of mixed working fluids on the thermodynamic performance of a throttling refrigeration cycle through experimental research. After optimizing the calculation of two ternary mixed working fluids with different compositions, the charging test is carried out, and the throttle characteristic curve of the mixed working fluid the law of temperature and pressure changes in the experiment was analyzed. The results show that: a mixed working fluid with a mixing ratio of 30.39mol% R14, 10.73mol% R170 and 58.87mol% R600A, at a pressure ratio of 2.5/0.1, a low temperature of -67.7°C is obtained, and the cooling time is 113.42min. The refrigeration system COP is 0.076; the mixing ratio is 30.39mol%R14, 15.73mol%R23 and 53.87mol%R600A mixed working fluid, in the case of a pressure ratio of 2.14/0.1, a low temperature of -76.4°C is obtained, and the cooling time is 160min, the refrigeration system COP is 0.106.