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Soil containing plant roots may be expected to exhibit a greater shearing resistance compared with the same ‘unreinforced’ soil, providing enhanced stability and effective erosion control, particularly for earth slopes. To be able to rely on the improved shearing resistance and stiffness of root-reinforced soils, it is important to understand and quantify the effectiveness of root reinforcement. This requires sophisticated multiscale models, building understanding at different length scales, from individual soil-root interaction through to full soil-profile or slope scale. One of the challenges with multiscale models is ensuring that they are representative of real behaviour, and this requires calibration to detailed high-quality experiments. The focus of the work presented was to capture and quantify root-reinforcement behaviour and associated soil and root deformation mechanisms during direct shear at the macroscopic to millimetre length scales. A novel shear box was developed to operate within a large-scale X-ray computed tomography (CT) scanner. Tests were interrupted to be scanned at a series of shear displacements from 0-20 mm to capture the chronology of behaviour in three-dimensions. Digital volume correlation (DVC) was applied to the CT dataset to obtain full-field 3D displacement and strain component information. The study demonstrates feasibility of the technique and presents preliminary DVC results.