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Injection of glucocorticoid hormone to adrenalectomized rats induces in liver microsomes a factor which stimulates polypeptide synthesis in a cell-free system. The factor is extractable from microsomes by 0.5 M KC1 followed by gel filtration, of the extract. Induction of the activator in glucocorticoid-treated rats contribute higher protein-synthesizing ability of the microsomes following hormone administration.

Four methods are described to obtain pure neuronal cultures from chick embryo cerebral hemispheres. In our experimental conditions the proliferation of the glial cells in inhibited and cultures of either low or high neuronal density are obtained. The isolated neurons, however, did not survive longer than 8 to 10 days; the contact with glial cells seems to be necessary for the maintenance of the neurons in long-term cultures.