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2 results on '"Koko, M"'

1. Valeur des tests PACE et CTB_ELISA dans le diagnostic de la peste porcine classique (PPC) et le contr{\^o}le de qualit{\'e} du vaccin correspondant {\`a} Madagascar

Author

Maminiaina, Of, Koko, M., Rajaonarison, J. J., Razafindrakoto, R., Ravaomanana, J., and Shannon, A. D.

Subjects
Quantitative Biology - Other Quantitative Biology
Abstract

From 1994, we began to use ELISA (Enzyme Linked Immunosorbent Assay) in the diagnosis of PCP. This is aELISA for capturing antigens (PACE) possibly contained in the samples. The advantage of this test comes from the fact that it is completelyindependent of cell cultures. In addition, it is fast: the result can be obtained in less than 36 hours. A study of its standardizationcarried out in Australia gave a sensitivity (Se) of 99%, a specificity (Sp) close to 100% and a negative predictive value (NPV) of 99.7%. Due to its high specificity, the test gives a negative result to all true negatives, in other words, the negatives of the test correspond to thetrue negatives. A variant of the capture ELISA, the CTB-ELISA or complex trapping blocking ELISA allows the quantity of antibodies to be measureddirected against the non-structural protein, p80 (or NS3), contained in animal sera. Evaluation of the level of anti-NS3 antibodiesconstitutes an excellent assessment of the level of neutralizing antibodies because the correlation coefficient between these two types of antibodies, the firstobtained by CTB-ELISA, and the second by serum neutralization (VNT), is very high (r = 0.98).The two tests being capable, one of detecting pestiviral antigens and the other of measuring antibodies specific to each of thegroups, constitutes an excellent tool for the qualitative control of anti-CSF vaccine., Comment: in French language

Published
2024

2. [Epidemiology of Newcastle disease in village poultry farming in Madagascar].

Author

Maminiaina OF, Koko M, Ravaomanana J, and Rakotonindrina SJ

Subjects
Animal Husbandry methods, Animals, Madagascar epidemiology, Newcastle Disease mortality, Newcastle Disease transmission, Poultry, Poultry Diseases mortality, Poultry Diseases transmission, Seroepidemiologic Studies, Antibodies, Viral blood, Newcastle Disease epidemiology, Newcastle disease virus immunology, Poultry Diseases epidemiology
Abstract

An epidemiological investigation into Newcastle disease in village poultry farming was carried out for 12 months (from May 1999 to June 2000) in Ambohimangakely and Moramanga, two agro-ecologic zones of Madagascar. The thirty families that were surveyed stated that they had incurred losses from an epizootic with high mortality rates at least once prior to the investigation. The results of serological tests and virus isolation showed that the disease, responsible for 44.3% of all the mortality recorded during the twelve-month period, was Newcastle disease. Maximum incidence of the disease (71%), affecting 75% of the families, occurred in October 1999, and seroprevalence often reached 100% after the outbreak had ended. The infection was brought to the villages either by newly introduced hens or recovered birds. All forms of Newcastle disease (epidemic, endemic and asymptomatic) were observed. The way farmers reacted contributed to the spread of the virus within a village and to neighbouring locations.

Published
2007

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