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Surface roughness is the key aspect which could increase the application of float glass by enhancing the machined hole quality. Glass is extensively used in microfluidic devices, bio-medical parts and biosensors. The core objective of the research study is to optimize the best parametric combination to achieve the least amount of surface roughness. The three major parameters which are used for designed experimental study are spindle speed, ultrasonic amplitude and feed rate. The least value of surface roughness is noticed at spindle speed (5000 rpm), vibration amplitude (20 μ m) and feed rate (6 mm/min) which be adopted for increasing its functional application. Consequently, after optimizing the parameters, least value of surface roughness at hole internal region is revealed as 1.09 μm.