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As one of the most successful techniques in experimental mechanics, digital image correlation (DIC) has been widely used to measure the motion and deformation of solid materials, especially in situations where non-contact measurement is required. This study describes and provides a comprehensive overview of the application of DIC using the open-source platform Ncorr on orthotropic materials. Orthotropic materials are a subset of anisotropic materials; their properties depend on the direction in which they are measured. Deformation displacement and in-plane strain in two directions are extracted from digital image correlation using a reference image recorded during the experiment. Tests are carried out on Laminated Veneer Lumber timber specimens according to the ASTM standard to determine the mechanical properties of materials under compression. DIC has proven to be a reliable, consistent, and cost-effective non-contact deformation measurement method that can assist in the extraction of mechanical properties of orthotropic materials. This study also showed that DIC analysis with a natural timber grain can be used to measure the displacement and strain fields of the material.