1. [Usefulness of a rapid intrapartum real-time PCR assay in comparison with the group B Streptococcus culture screening at the end of pregnancy in pregnant women].
- Author
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Defez M, Khizar F, Maurin M, Biot F, Pons JC, and Sergent F
- Subjects
- Adult, Female, Humans, Pregnancy, Sensitivity and Specificity, Time Factors, Labor, Obstetric, Pregnancy Complications, Infectious diagnosis, Real-Time Polymerase Chain Reaction standards, Streptococcal Infections diagnosis
- Abstract
Objectives: The objectives were to evaluate and compare the diagnostic accuracy of a rapid real-time PCR assay at the onset of labor with those of the current antenatal culture-based test at 34-38 weeks gestation for group B Streptococcus (GBS) screening., Materials and Methods: A prospective study including all pregnant women admitted for delivery after a 34-week gestation period was conducted in October 2012 at the Grenoble University Hospital Centre. A first culture-based GBS screening test was performed between 34 and 38 weeks of gestation followed by a second screening test at the onset of labor, using a real-time PCR Assay and a culture-based method (gold standard) in order to calculate the diagnostic accuracy., Results: One hundred an fifty-seven patients were enrolled. The sensitivity was 94.4% (95% CI, 72.7-99.9%) with intrapartum PCR assay and 50% (95% CI, 26-74%) with antepartum culture. Prevalence of GBS colonization was 7.6% with the antepartum culture method, 11.5% with intrapartum culture and 16.6% by using PCR-test., Conclusion: Intrapartum PCR shows a much higher sensitivity compared to the antepartum culture-based screening mainly due to variations in GBS colonization and could allow us to target patients requiring intrapartum antibiotic prophylaxis more effectively., (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)
- Published
- 2016
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