1. Extended delivery of vaccines to the skin improves immune responses.
- Author
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Joyce JC, Sella HE, Jost H, Mistilis MJ, Esser ES, Pradhan P, Toy R, Collins ML, Rota PA, Roy K, Skountzou I, Compans RW, Oberste MS, Weldon WC, Norman JJ, and Prausnitz MR
- Subjects
- Animals, Antigens immunology, Female, Immunity, Humoral immunology, Immunization Schedule, Immunologic Memory, Injections, Intradermal, Mice, Mice, Inbred BALB C, Rats, Rats, Wistar, Sigmodontinae, Time Factors, Vaccines immunology, Antibodies, Neutralizing immunology, Antigens administration & dosage, Vaccines administration & dosage
- Abstract
Vaccines prevent 2-3 million childhood deaths annually; however, low vaccine efficacy and the resulting need for booster doses create gaps in immunization coverage. In this translational study, we explore the benefits of extended release of licensed vaccine antigens into skin to increase immune responses after a single dose in order to design improved vaccine delivery systems. By administering daily intradermal injections of inactivated polio vaccine according to six different delivery profiles, zeroth-order release over 28 days resulted in neutralizing antibody titers equivalent to two bolus vaccinations administered one month apart. Vaccinations following this profile also improved immune responses to tetanus toxoid and subunit influenza vaccine but not a live-attenuated viral vaccine, measles vaccine. Finally, using subunit influenza vaccine, we demonstrated that daily vaccination by microneedle patch induced a potent, balanced humoral immunity with an increased memory response compared to bolus vaccination. We conclude that extended presentation of antigen in skin via intradermal injection or microneedle patch can enhance immune responses and reduce the number of vaccine doses, thereby enabling increased vaccination efficacy., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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