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Abstract Background Enhancer RNAs (eRNAs) play a crucial role in transcriptional regulation. While significant progress has been made in understanding epigenetic regulation mediated by eRNAs, research on the construction of eRNA-mediated gene regulatory networks (eGRN) and the identification of critical network components that influence complex traits is lacking. Results Here, employing the pig as a model, we conducted a comprehensive study using H3K27ac histone ChIP-seq and RNA-seq data to construct eRNA expression profiles from multiple tissues of two distinct pig breeds, namely Enshi Black (ES) and Duroc. In addition to revealing the regulatory landscape of eRNAs at the tissue level, we developed an innovative network construction and refinement method by integrating RNA-seq, ChIP-seq, genome-wide association study (GWAS) signals and enhancer-modulating effects of single nucleotide polymorphisms (SNPs) measured by self-transcribing active regulatory region sequencing (STARR-seq) experiments. Using this approach, we unraveled eGRN that significantly influence the growth and development of muscle and fat tissues, and identified several novel genes that affect adipocyte differentiation in a cell line model. Conclusions Our work not only provides novel insights into the genetic basis of economic pig traits, but also offers a generalizable approach to elucidate the eRNA-mediated transcriptional regulation underlying a wide spectrum of complex traits for diverse organisms.

This article focuses on residential buildings in the Hefei region. Energy consumption simulations for different building layouts were conducted using ECOTECT and BECH software. Comparative analysis of simulation results was performed for three different conditions: winter, summer, and transitional seasons. The study aimed to identify the most energy-efficient building layout considering the local climate. The results indicate that for winter and summer conditions, the row-column layout is the most energy-efficient, saving 7.66% and 8.33% compared to enclosed and slanted layouts in winter, and 4.98% and 5.07% in summer. However, during the transitional season, an enclosed layout in April achieves the best energy-saving performance, while in October, the enclosed layout consumes 2.82% more energy than the row-column and slanted layouts. Overall, considering annual energy consumption, the row-column layout proves to be the most energy-efficient for residential buildings in the Hefei region, with energy savings of 4.28% and 3.5% compared to enclosed and slanted layouts, respectively, providing valuable in sights for energy-efficient design in residential buildings.

Our laboratory focuses on chemical proteomics-enabled discovery of new cysteine-reactive small molecules with intriguing biomedical activities as well as identification and detailed characterization of their proteomic targets. In this overview article, we summarize our progress since 2013 in this research field. We have developed a novel mass spectrometry-based chemoproteomic method that allows detection and monitoring of up to ~3000 reactive cysteines in any cellular proteome. This is achieved via strategic use of two clickable, cysteine-reactive chemical probes with complementary substrate selectivity profiles, iodoacetamide and ethynyl benziodoxolone. Using this method, we have been able to identify the direct biological targets of curcumin, a diarylheptanoid natural product with anticancer activity, and deoxyelephantopin, a highly cytotoxic natural sesquiterpene lactone. Furthermore, we have developed chloromethyl triazoles (CMTs) as a novel chemical scaffold for cysteine-reactive inhibitors that can be accessed from commercially available substrates in only two chemical steps. From a small collection of chloromethyl triazoles, we have identified compound AA-CW236 as the first non-pseudosubstrate inhibitor of MGMT, a DNA repair protein that renders several devastating cancer forms resistant to chemotherapy.

Aiming at the problem of link congestion caused by the shortage of network bandwidth resources at the user end, this paper first proposes a regional load balancing idea. Then, for the problem of bandwidth resource allocation in regional load balancing, a bandwidth allocation model is established and a dynamic auction algorithm is proposed. The algorithm calculates the link quality and stability by constructing a link model, and introduces the auction bandwidth to the auctioneer's incentive degree to obtain the auction bidding function. The simulation results show that the algorithm can effectively improve the user's network status, reduce the service response delay, increase the throughput, and at the same time can effectively prevent the auction user's false bidding behavior, so that the auction quote quickly converges to the maximum quote, reduces the number of auctions, and reduces Communication overhead.

Some of triboluminescence materials have the property of shortwave light emission, which is possible to make them being used as the light resource of pest-insects phototaxis trapping. The inorganic composite phosphors Sr2MgSi2O7:Ce and organic composite phosphor Mn-PMBB are tested to have the violet-blue spectrum and green spectrum glowing respectively; Their vertical axis wind driving triboluminescence unit is designed and fabricated on the basis of squirrel cage structure friction pair with multi-glass bars against on the cylindrical phosphor. The persistant wind driving tribo-luminescence is obtained in the experiment of wind driving triboluminescence unit. Further the triboplasma of N2 and N2- Ar gases closed in the quartz tube is researched by means of PTFE elctret on quartz tube friction pair, and a high intensity triboplasma light emission with more than 50000 counts is obtained in the span of 310-420nm spectrum that supplies a more suitable shortwave spectrum of phototaxis trapping pest insects. The annular quartz glass tube is designed to constitute PTFE against on quartz friction pair; The triboplasma emission device is fabricated utilizing three stacking layer structure of PTFE-anuular quartz tube friction pairs, and the S-type vertical shaft wind tuebine is used to form the wind driving triboplasma emission unit. The violet-blue emission spectrum of N2-Ar gas troboplasma is obtained in the test of vertical wind driving triboplasma unit.