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2 results on '"Cell Analysis"'

1. TUNABLE ELECTROMAGNETIC BIOSENSORS APPLICATED TO CELLULAR ANALYSIS.

Author

LANDOULSI, A., ZHANG, L. Y., LACROIX, A., MORAND DU PUCH, C. BOUNAIX, DALMAY, C., POTHIER, A., BESSAUDOU, A., LAUTRETTE, C., BATTU, S., LALLOUE, F., JAUBERTEAU, M. O., and BLONDY, P.

Subjects
BIOSENSOR research, ELECTROMAGNETISM, CELL analysis, MICROWAVES, CANCER cells
Abstract

The interest of using of microwave in the biomedical field is growing since few years, especially for application dedicated to cellular analysis. Indeed, due to its size and cytoplasm content, a biological cell can be considered as a micrometric dielectric particle. Probing this cell thanks to microwave signal can allow to access to data on its biological and physical content. This paper illustrates the interaction capabilities of microwave with cell cytoplasm in order to extract their intrinsic dielectric properties. In this objective, some tunable resonant frequency biosensors have been designed in order to characterize cancer cells. [ABSTRACT FROM AUTHOR]

Published
2013

2. OPTIMISATION DE L'ANALYSE GENETIQUE DE LA CELLULE UNIQUE.

Author

MOUAWIA, Hussein and SAKER, Ali

Subjects
*MOLECULAR genetics, *CELL analysis, *DNA structure, *MOLECULAR structure of RNA, *POLYMERASE chain reaction
Abstract

The molecular genetic analysis of microdissected cells by laser, a method for selecting a starting material of pure DNA or RNA uncontaminated. Our study focuses on technical pre-PCR (polymerase chain reaction) for the amplification of DNA from a single cell (leukocyte) isolated from human blood after laser microdissection and aims to optimize the yield of DNA extracted of this cell to be amplified without errors and provide reliable genetic analyzes. This study has allowed us to reduce the duration of cell lysis in order to perform the step of expanding genomic PEP (primer extension preamplification) directly after lysis the same day and the quality of genomic amplification and eliminate purification step of the product PEP, step with a risk of contamination and risk of loss of genetic material related to manipulation. This approach has shown that the combination of at least 3 STR (short tandem repeat) markers for genetic analysis of single cell improves the efficiency and accuracy of PCR and minimizes the loss of allele (allele drop out; ADO). This protocol can be applied to large scale and an effective means suitable for genetic testing for molecular diagnostic from isolated single cell (cancerous - fetal). [ABSTRACT FROM AUTHOR]

Published
2012

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