1. [Apolipoprotein(a) isoforms immunoblotting detection: comparative study of two methods].
- Author
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Edjème-Aké A, Garnotel R, Vallé-Polneau S, Ahiboh H, Hauhouot-Attoungbré ML, Monnet D, and Gillery P
- Subjects
- Adolescent, Adult, Apoprotein(a) blood, Blood Donors, Colorimetry methods, Cote d'Ivoire, Electrophoresis, Polyacrylamide Gel, Humans, Luminescent Measurements methods, Middle Aged, Molecular Weight, Protein Isoforms analysis, Protein Isoforms metabolism, Sensitivity and Specificity, Young Adult, Apoprotein(a) analysis, Apoprotein(a) metabolism, Immunoblotting methods
- Abstract
This study reports the comparison between two methods (chemiluminescence and enzymatic colorimetry) for revelation of apolipoprotein(a) [apo(a)] isoforms by immunoblotting in 102 Ivorian healthy subjects. Apo(a) isoform sizes were determined by sodium dodecyl sulfate-agarose-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting using enzymatic colorimetry or chemiluminescence. Within-run precision was comprised between 4.9% and 9.2% for colorimetry and between 2.9% and 4.6% for chemiluminescence. Both methods have detected apo(a) isoforms in all patients, even when lipoprotein(a) concentrations were under detection limit (0.02 g/L). The two methods were significantly correlated (r = 0.96 to 0.98, p<0.0001). Even though the chemiluminescence method exhibited better performances than the colorimetric method, both techniques could be used indifferently.
- Published
- 2012
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