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The Aristotelia chilensis (Mol.) Stuntz, also known as Maqui, is an endemic berry native to southern Chile. It is a very popular berry for its nutritional attributes and health benefits, provided mainly by its polyphenols. This review aims to investigate the Maqui and its nutritional characteristics, its health benefits, and the application of Maqui in the food industry. This fruit provides 150 calories per 100 g of product and has a low protein content and a high fiber content. Its seeds contain monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs); however, its most outstanding feature is its high value of bioactive compounds, mainly anthocyanins, indole alkaloids and flavonoids, coumarins, caffeic and ferulic acids, and delphinidin 3-O-β-glucoside, the latter being the most representative, providing Maqui with high antioxidant activity. Maqui is considered a fruit of high interest as a nutraceutical product for the control and prevention of ongoing diseases, and among its benefits, we can highlight glycemic and metabolic control; the control and prevention of obesity, cancer, cognitive decline, and dementia; the prevention and treatment of bone structure alterations; prevention against oxidative stress, particularly in cigarette smoke-induced stress. In addition to its nutraceutical use, Maqui has been used in the food industry to improve the shelf life (by controlling lipid oxidation) and nutritional value of food products and as a substitute for synthetic additives. In addition, the inclusion of Maqui improves the organoleptic and sensory characteristics of foods. The incorporation of this fruit has been observed mainly in drinks, meat products, bakery products, and milk products. Evidence has shown that Maqui consumption, as well as products with Maqui added, have a good acceptability and exert benefits on people's health. Knowledge about the application of Maqui in food will allow us to create new nutraceutical and food products that improve their nutritional and functional value. [ABSTRACT FROM AUTHOR]

Introduction: Crohn's disease (CD) involves activation of mast cells (MC) and NF-κB in parallel with the PPAR-α/NLRP3 inflammasome/IL-1β pathway in the inflamed colon. Whether polyphenols from maqui (Aristotelia chilensis) represent a natural alternative treatment for CD is unclear. Therefore, we used an animal model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD-like colitis to investigate protective effects of maqui extract through monitoring NLRP3 inflammasome and MC activation in colon tissue. Methods: Maqui extract was administered via orogastric route to mice after (post-Treatment group) or prior (pre-Treatment group) to TNBS-induction. Colon pathology was characterized by histoarchitectural imaging, disease activity index (DAI), and assessing NF-κB, p-NF-κB, PPAR-α/NLRP3 expression and IL-1b levels. Results: Compared to mice treated with TNBS alone administration of anthocyanin-rich maqui extract improved the DAI, colon histoarchitecture and reduced both colon wet-weight and transmural inflammation. Induction with TNBS significantly increased colonic NLPR3 inflammasome activation, while cotreatment with maqui extract (either post- or pre-Treatment) significantly downregulated NLRP3, ASC and caspase-1 levels, which manifested as reduced colonic IL-1β levels. Supplemented maqui extract marginally diminished NF-κB activity in epithelial cells but reached statistical significance in immune cells (as judged by decreased NF-κB phosphorylation). PPAR-α signaling was largely unaffected by Maqui whereas MC infiltration into the colon mucosa and submucosa decreased and their level of degranulation was suppressed. Conclusion: These outcomes show the post- and pre-Treatment effect of a polyphenolic extract rich in anthocyanins from maqui the acute phase of TNBSinduced CD-like colitis is linked to suppression of the NLRP3 inflammasome and reduced MC responses. These data indicate that maqui extract represents a potential nutraceutical for the treatment of inflammatory bowel disease (IBD). [ABSTRACT FROM AUTHOR]

IntroductionCrohn’s disease (CD) involves activation of mast cells (MC) and NF-кB in parallel with the PPAR-α/NLRP3 inflammasome/IL-1β pathway in the inflamed colon. Whether polyphenols from maqui (Aristotelia chilensis) represent a natural alternative treatment for CD is unclear. Therefore, we used an animal model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD-like colitis to investigate protective effects of maqui extract through monitoring NLRP3 inflammasome and MC activation in colon tissue.MethodsMaqui extract was administered via orogastric route to mice after (post-Treatment group) or prior (pre-Treatment group) to TNBS-induction. Colon pathology was characterized by histoarchitectural imaging, disease activity index (DAI), and assessing NF-кB, p-NF-кB, PPAR-α/NLRP3 expression and IL-1β levels.ResultsCompared to mice treated with TNBS alone administration of anthocyanin-rich maqui extract improved the DAI, colon histoarchitecture and reduced both colon wet-weight and transmural inflammation. Induction with TNBS significantly increased colonic NLPR3 inflammasome activation, while co-treatment with maqui extract (either post- or pre-Treatment) significantly downregulated NLRP3, ASC and caspase-1 levels, which manifested as reduced colonic IL-1β levels. Supplemented maqui extract marginally diminished NF-кB activity in epithelial cells but reached statistical significance in immune cells (as judged by decreased NF-кB phosphorylation). PPAR-α signaling was largely unaffected by Maqui whereas MC infiltration into the colon mucosa and submucosa decreased and their level of degranulation was suppressed.ConclusionThese outcomes show the post- and pre- Treatment effect of a polyphenolic extract rich in anthocyanins from maqui the acute phase of TNBS- induced CD-like colitis is linked to suppression of the NLRP3 inflammasome and reduced MC responses. These data indicate that maqui extract represents a potential nutraceutical for the treatment of inflammatory bowel disease (IBD).

Maqui berry (Aristotelia chilensis) is characterized by antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effect of maqui berry extracts on human skin fibroblasts (NHSFs) exposed to ultraviolet radiation (UVB). The photoprotective properties of the extracts were investigated via the determination of the total polyphenolic content (TPC) and antioxidant capacity (DPPH), and the chemical composition was assessed. The chemical purity of the extracts was studied via the evaluation of the toxic elements level. The water extract (MWE 57.75 ± 0.44 mg GAE/g) had the highest mean polyphenol content. The water (MWE) and ethanol (MEE70) extracts had the highest inhibitory activities against DPPH radical formation (283.63 ± 7.29 and 284.60 ± 4.31 mg Tx/L, respectively). The analyzed extracts were found to be safe in terms of toxic elements (arsenic, cadmium, lead). The tested extracts of maqui berry did not cause a cytotoxic effect on NHSF cells after 24, 48, and 72 h of incubation. When the NHSF cells were exposed to UVB radiation in the presence of maqui extracts, their viability was increased or maintained. The maqui berry extracts had a slightly protective effect against skin damage caused by UVB radiation. These were preliminary studies that require further research to determine which maqui compounds correspond with the photoprotective activity. [ABSTRACT FROM AUTHOR]

To assess the immediate/short-term outcomes of a maqui berry-based nutraceutical (MBN) supplementation on the autonomic nervous system (ANS), resilience level, metabolism and body composition. A randomized controlled single-blind study was performed on healthy subjects. Throughout 30 days, one group took 1,000 mg/day of an MBN (Maqui 500®), the control group had no supplementation. On day 0 (T0) and 30 (T3) all subjects performed blood tests, bioimpedance spectroscopy and questionnaires for resilience, perceived stress and short-form 12 (SF12). At T0, 75′ after T0 (T1), on day 7 and at T3 the subjects underwent biometric parameter measurement and heart rate variability (HRV) test to investigate psycho-physical resilience. Fifteen subjects per group were included; abnormal seasonal high temperatures altered individuals' lifestyle and nutrition, influencing the trial's outcomes. Biometric parameters, blood pressure, oxygen saturation and blood tests did not differ between T0 and T3 in both groups. In the MBN group the HRV analysis showed a significant increase of ANS coordination (p=0.05), parasympathetic activity at 75′, very low frequencies and total power at T3, whereas these parameters decreased in the control group. SF12 mental score improved in the maqui group (p=0.02); the questionnaire-based outcomes showed no further variations. In the control subjects bioimpedance showed an increase of resistance and fat mass, with decreased total body water and lean mass (p=n.s.). The maqui-based nutraceutical improved HRV, namely ANS activation/coordination, and SF12 mental component. Blood tests and bioimpedance/biometric parameters mildly varied. The elapsed hot weather likely biased many investigated variables. [ABSTRACT FROM AUTHOR]

Metabolic diseases have been related to the overdrinking of high-sugar content beverages. As a result, the demand for alternative formulations based on plant-based ingredients with health-promoting properties has increased during the last few years. Nonetheless, the design and production of effective formulations requires understanding the bioavailability of these compounds. For this purpose, a two-month longitudinal trial with 140 volunteers was conducted to measure the beneficial effects of a maqui–citrus beverage, rich in (poly)phenols. From data obtained by quantifying metabolites present in urine samples, biostatistical and machine learning (data imputation, feature selection, and clustering) methods were applied to assess whether a volunteer's sex and the sweetener added to the beverage (sucrose, sucralose, or stevia) affected the bioavailability of (poly)phenol metabolites. Several metabolites have been described as being differentially influenced: 3,4-dihydroxyphenylacetic acid and naringenin with its derivatives were positively influenced by stevia and men, while eriodictyol sulfate and homoeridictyol glucunoride concentrations were enhanced with stevia and women. By examining groups of volunteers created by clustering analysis, patterns in metabolites' bioavailability distribution as a function of sex and/or sweeteners (or even due to an uncontrolled factor) were also discovered. These results underline the potential of stevia as a (poly)phenol bioavailability enhancer. Furthermore, they also evidence sex affects the bioavailability of (poly)phenols, pointing at a sex-dependent metabolic pathway regulation. [ABSTRACT FROM AUTHOR]

The Aristotelia chilensis (Mol.) Stuntz, also known as Maqui, is an endemic berry native to southern Chile. It is a very popular berry for its nutritional attributes and health benefits, provided mainly by its polyphenols. This review aims to investigate the Maqui and its nutritional characteristics, its health benefits, and the application of Maqui in the food industry. This fruit provides 150 calories per 100 g of product and has a low protein content and a high fiber content. Its seeds contain monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs); however, its most outstanding feature is its high value of bioactive compounds, mainly anthocyanins, indole alkaloids and flavonoids, coumarins, caffeic and ferulic acids, and delphinidin 3-O-β-glucoside, the latter being the most representative, providing Maqui with high antioxidant activity. Maqui is considered a fruit of high interest as a nutraceutical product for the control and prevention of ongoing diseases, and among its benefits, we can highlight glycemic and metabolic control; the control and prevention of obesity, cancer, cognitive decline, and dementia; the prevention and treatment of bone structure alterations; prevention against oxidative stress, particularly in cigarette smoke-induced stress. In addition to its nutraceutical use, Maqui has been used in the food industry to improve the shelf life (by controlling lipid oxidation) and nutritional value of food products and as a substitute for synthetic additives. In addition, the inclusion of Maqui improves the organoleptic and sensory characteristics of foods. The incorporation of this fruit has been observed mainly in drinks, meat products, bakery products, and milk products. Evidence has shown that Maqui consumption, as well as products with Maqui added, have a good acceptability and exert benefits on people’s health. Knowledge about the application of Maqui in food will allow us to create new nutraceutical and food products that improve their nutritional and functional value.

Maqui berry (Aristotelia chilensis) is characterized by antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effect of maqui berry extracts on human skin fibroblasts (NHSFs) exposed to ultraviolet radiation (UVB). The photoprotective properties of the extracts were investigated via the determination of the total polyphenolic content (TPC) and antioxidant capacity (DPPH), and the chemical composition was assessed. The chemical purity of the extracts was studied via the evaluation of the toxic elements level. The water extract (MWE 57.75 ± 0.44 mg GAE/g) had the highest mean polyphenol content. The water (MWE) and ethanol (MEE70) extracts had the highest inhibitory activities against DPPH radical formation (283.63 ± 7.29 and 284.60 ± 4.31 mg Tx/L, respectively). The analyzed extracts were found to be safe in terms of toxic elements (arsenic, cadmium, lead). The tested extracts of maqui berry did not cause a cytotoxic effect on NHSF cells after 24, 48, and 72 h of incubation. When the NHSF cells were exposed to UVB radiation in the presence of maqui extracts, their viability was increased or maintained. The maqui berry extracts had a slightly protective effect against skin damage caused by UVB radiation. These were preliminary studies that require further research to determine which maqui compounds correspond with the photoprotective activity.

Some fruits and vegetables, rich in bioactive compounds such as polyphenols, flavonoids, and anthocyanins, may inhibit platelet activation pathways and therefore reduce the risk of suffering from CVD when consumed regularly. Aristotelia chilensis Stuntz (Maqui) is a shrub or tree native to Chile with outstanding antioxidant activity, associated with its high content in anthocyanins, polyphenols, and flavonoids. Previous studies reveal different pharmacological properties for this berry, but its cardioprotective potential has been little studied. Despite having an abundant composition, and being rich in bioactive products with an antiplatelet role, there are few studies linking this berry with antiplatelet activity. This review summarizes and discusses relevant information on the cardioprotective potential of Maqui, based on its composition of bioactive compounds, mainly as a nutraceutical antiplatelet agent. Articles published between 2000 and 2022 in the following bibliographic databases were selected: PubMed, ScienceDirect, and Google Scholar. Our search revealed that Maqui is a promising cardiovascular target since extracts from this berry have direct effects on the reduction in cardiovascular risk factors (glucose index, obesity, diabetes, among others). Although studies on antiplatelet activity in this fruit are recent, its rich chemical composition clearly shows that the presence of chemical compounds (anthocyanins, flavonoids, phenolic acids, among others) with high antiplatelet potential can provide this berry with antiplatelet properties. These bioactive compounds have antiplatelet effects with multiple targets in the platelet, particularly, they have been related to the inhibition of thromboxane, thrombin, ADP, and GPVI receptors, or through the pathways by which these receptors stimulate platelet aggregation. Detailed studies are needed to clarify this gap in the literature, as well as to specifically evaluate the mechanism of action of Maqui extracts, due to the presence of phenolic compounds. [ABSTRACT FROM AUTHOR]

Metabolic diseases have been related to the overdrinking of high-sugar content beverages. As a result, the demand for alternative formulations based on plant-based ingredients with health-promoting properties has increased during the last few years. Nonetheless, the design and production of effective formulations requires understanding the bioavailability of these compounds. For this purpose, a two-month longitudinal trial with 140 volunteers was conducted to measure the beneficial effects of a maqui–citrus beverage, rich in (poly)phenols. From data obtained by quantifying metabolites present in urine samples, biostatistical and machine learning (data imputation, feature selection, and clustering) methods were applied to assess whether a volunteer’s sex and the sweetener added to the beverage (sucrose, sucralose, or stevia) affected the bioavailability of (poly)phenol metabolites. Several metabolites have been described as being differentially influenced: 3,4-dihydroxyphenylacetic acid and naringenin with its derivatives were positively influenced by stevia and men, while eriodictyol sulfate and homoeridictyol glucunoride concentrations were enhanced with stevia and women. By examining groups of volunteers created by clustering analysis, patterns in metabolites’ bioavailability distribution as a function of sex and/or sweeteners (or even due to an uncontrolled factor) were also discovered. These results underline the potential of stevia as a (poly)phenol bioavailability enhancer. Furthermore, they also evidence sex affects the bioavailability of (poly)phenols, pointing at a sex-dependent metabolic pathway regulation.

This work aimed at developing powders rich in antioxidants and pigments from two wild berries: maqui (Aristotelia chilensis) and murra (Rubus ulmifolius). Fruits were subjected to successive ultrasound-assisted extractions (UAE) and then freeze-dried. Physical properties, anthocyanin stability of powders, and their performance as natural colorants in yogurts were evaluated. The optimum extraction methods were: UAE for 10 min in murra, and without UAE (control) in maqui, with juice extraction yields ranging between 80 and 82%. Maqui powder exhibited ≈ 2.8 times more polyphenol and anthocyanin content than murra. However, murra powder showed better stability characteristics as powder colorant since it exhibited greater protection of anthocyanins by means of copigmentation phenomena. Regarding consumer's perception of colored yogurt, samples with 4% and 8% maqui powder could be considered as future prototypes to be launched into the market. The obtained powders may be used in different industrial food applications. [ABSTRACT FROM AUTHOR]

Some fruits and vegetables, rich in bioactive compounds such as polyphenols, flavonoids, and anthocyanins, may inhibit platelet activation pathways and therefore reduce the risk of suffering from CVD when consumed regularly. Aristotelia chilensis Stuntz (Maqui) is a shrub or tree native to Chile with outstanding antioxidant activity, associated with its high content in anthocyanins, polyphenols, and flavonoids. Previous studies reveal different pharmacological properties for this berry, but its cardioprotective potential has been little studied. Despite having an abundant composition, and being rich in bioactive products with an antiplatelet role, there are few studies linking this berry with antiplatelet activity. This review summarizes and discusses relevant information on the cardioprotective potential of Maqui, based on its composition of bioactive compounds, mainly as a nutraceutical antiplatelet agent. Articles published between 2000 and 2022 in the following bibliographic databases were selected: PubMed, ScienceDirect, and Google Scholar. Our search revealed that Maqui is a promising cardiovascular target since extracts from this berry have direct effects on the reduction in cardiovascular risk factors (glucose index, obesity, diabetes, among others). Although studies on antiplatelet activity in this fruit are recent, its rich chemical composition clearly shows that the presence of chemical compounds (anthocyanins, flavonoids, phenolic acids, among others) with high antiplatelet potential can provide this berry with antiplatelet properties. These bioactive compounds have antiplatelet effects with multiple targets in the platelet, particularly, they have been related to the inhibition of thromboxane, thrombin, ADP, and GPVI receptors, or through the pathways by which these receptors stimulate platelet aggregation. Detailed studies are needed to clarify this gap in the literature, as well as to specifically evaluate the mechanism of action of Maqui extracts, due to the presence of phenolic compounds.

Cancer of the reproductive tract includes diseases with higher prevalence in the female population. This investigation examined whether an anthocyanin-enriched extract of Aristotelia chilensis, commonly known as "maqui," could affect some hallmarks of endometrial cancer. Cultures of the human endometrial cancer cell line Ishikawa were treated with a hydroethanolic maqui extract at 1, 3, 10, 30, 100, 300, or 1000 µg/mL to determine the effect on cell viability by MTT assay. Then, we used the 50% Effective Concentration (EC50) to evaluate whether the effect of the maqui extract is mediated via an arrest of the cell cycle or induction of apoptosis using flow cytometry or Annexin V -FITC assays, respectively. The effects of sublethal doses of the maqui extract on migration and invasiveness of Ishikawa cells were also evaluated by the wound healing and Boyden Chamber assay, respectively. Our results show that the hydroethanolic maqui extract inhibits the cell viability with an EC50 of 472.3 µg/mL via increased apoptosis, and that reduces the invasive capacity but not migration of Ishikawa cells. These findings suggest that the hydroethanolic maqui extract has antineoplastic properties for endometrial cancer and merits further studies to corroborate its efficiency as anticancer therapy in reproductive organs. [ABSTRACT FROM AUTHOR]

Background: Heart Rate Variability (HRV) is a parameter used to investigate the autonomous neural system (sympathetic and parasympathetic system). Reduced HRV is a risk factor which correlates with physical and psychological stress, psychoneuroendocrineimmunology dysregulation as well as with aging. HRV is considered a reliable parameter to investigate psycho-physical resilience, the latter being considered a key-factor for human longevity and the final target of hormesis pathways as well. Different chemical, physical, psychological stressors may interfere with HRV. Aims: The aim of our observational study was to assess the possible changes in HRV changes induced by a few targeted stimulations: breathing, maqui nutraceutical intake, physical activity and thermal stress, and maqui nutraceutical intake. Patients and Methods: An observational study was performed on 4 groups of subjects, for a total of 112 individuals, who underwent HRV analysis after administration of different stressors/stimuli, such as breathing, thermal stimulation, Maqui 500 ® nutraceutical intake and physical activity. The investigated parameters were: SDNN, RMSSD, MHRR, mean heart rate (MHR) and the three frequency-domain based bands (VLF, LF, HF). Results: Our outcomes demonstrate that HRV assessment is a useful and reliable investigation to highlight and monitor the effects of these stressors. Breathing was the stimulus which induced the most significant variation in HV parameters. Conclusions: Notwithstanding the short duration of the follow-up, immediate/short-term HRV assessment showed statistically significant variations of the main parameters (e.g. SDNN, RMSSD and frequency bands) in a few cases after stimuli exposure. Studies including larger cohorts and longer follow-up are needed and justified to corroborate our outcomes furthermore.

Maqui (Aristotelia chilensis [Molina] Stuntz) is a dioecious small tree native to Chile and southwestern Argentina. This species has gained attention due to its high polyphenol content and anti-oxidant capacity. Nevertheless, genetics studies and information about A. chilensis population genetics are scarce and even contradictory. In fact, the available species-specific simple sequence repeat (SSR) markers are not informative at all, and so we decided to identify and characterize new ones able to trace individual genotypes, a basic tool intended for different genetic studies. We identified and characterized 15 new polymorphic SSR markers for A. chilensis. These markers were evaluated in three populations distributed along 1000 km of Central Chile, exhibiting up to 10 alleles per locus and a combined expected heterozygosity of 0.858. Markers were also informative in two related species, Aristotelia peduncularis (Labill.) Hook. f. and Crinodendron patagua Molina (Elaeocarpaceae), with 13 and six SSRs showing clear amplification patterns, respectively. This new set of SSR markers are highly polymorphic and informative, being the first ones available for the effective fingerprinting of maqui genotypes. A proof of concept of that was the differentiation of six maqui accessions that are under domestication for productive purposes, based on a subset of the polymorphic SSR markers. [ABSTRACT FROM AUTHOR]

Aristotelia chilensis is a plant rich in phenolics and other bioactive compounds. Their leaves are discarded as waste in the maqui berry industry. A new application of these wastes is intended by the recovery of bioactive compounds using pressurized hot water extraction with conventional or microwave heating. Both technologies have been selected for their green character regarding the type of solvent and the high efficiency in shorter operation times. Extractions were performed in the temperature range 140–200 °C with a solid/liquid ratio of 1:15 (w:w). The extracts’ total phenolic content, antioxidant capacity, and saccharides content obtained with both heating methods were measured. Additionally, the thermo-rheological properties of the gelling matrix enriched with these extracts were analyzed. Optimum conditions for lyophilized extracts were found with conventional heating, at 140 °C and 20 min extraction; 250.0 mg GAE/g dry extract and 1321.5 mg Trolox/g dry extract. Close to optimum performance was achieved with microwave heating in a fraction of the time (5 min) at 160 °C (extraction), yielding extracts with 231.9 mg GAE/g dry extract of total phenolics and antiradical capacity equivalent to 1176.3 mg Trolox/g dry extract. Slightly higher antioxidant values were identified for spray-dried extracts (between 5% for phenolic content and 2.5% for antioxidant capacity). The extracts obtained with both heating methods at 200 °C contained more than 20% oligosaccharides, primarily glucose. All the formulated gelling matrices enriched with the obtained extracts displayed intermediate gel strength properties. The tested technologies efficiently recovered highly active antioxidant extracts, rich in polyphenolics, and valuable for formulating gelling matrices with potential applicability in foods and other products.

The study evaluated the effects of nucleoprotein viral and the infectious virus in SHK-1 cells. The results show a strong respiratory burst activation and the induction of p47phox, SOD, GLURED, and apoptotic genes. Additionally, the cells alter the profile of SUMOylated proteins by the effect of transfection and infection experiments. In silico analyses show a set of structural motifs in NP susceptible of post-translational modification by the SUMO protein. Interestingly, the inhibition of the NADPH oxidase complex blocked the production of reactive oxygen species and the high level of cellular ROS due to the nucleoprotein and the ISAv. At the same time, the blocking of the p38MAPK signaling pathway and the use of Aristotelia chilensis, decreased viral progeny production. These results suggest that the NP triggers a strong production of ROS and modifying the post-translational profile mediated by SUMO-2/3, a phenomenon that favors the production of new virions. [ABSTRACT FROM AUTHOR]

Maqui (Aristotelia chilensis (Molina) Stuntz) is a native Chilean berry that has the highest antioxidant level compared with other fruit. Anthocyanins, which are the compound with the highest functional level found in maqui, are relatively unstable and quite susceptible to degradation during processing and storage. In addition, maqui is a highly perishable seasonal fruit and it is necessary to find conservation methods for the developed products. Microencapsulation is one way to protect anthocyanins from degradation reactions; it is defined as a process by which certain bioactive substances are introduced in a matrix or wall systems aimed at impeding their loss and protecting them from the reaction with other compounds and/or prevent them from suffering oxidation reactions caused by light or oxygen. The objective of the present study was to evaluate the effect of drying inlet temperature on the physical properties, total polyphenol content, total anthocyanin content, and antioxidant activity of spray-dried maqui extract. Inlet temperatures were 130, 150, and 170 °C, while other parameters were constant, such as feed flow (18 mL min-1) and encapsulating agent concentration (maltodextrin and gum Arabic). The best drying inlet temperature was 170 °C; it produced powders with lower moisture content (1.61%), water activity (0.15), and L* coordinate (11.16), as well as increased hygroscopicity (24.01%) and solubility (92.70%). The lowest total polyphenol content loss (23.05 mg gallic acid equivalents g-1), total anthocyanin content (21.46 mg cyanidin-3-glucoside g-1), and antioxidant activity (85.76% 2,2-diphenyl-1-picrylhydrazyl inhibition) occurred at 170 °C. The size of the powder particles allowed classifying them as microcapsules. Maqui extract microencapsulation provides powders with adequate stability during storage. [ABSTRACT FROM AUTHOR]

Sugar intake abuse is directly related with the increase of metabolic diseases such as type 2 diabetes, obesity, and insulin resistance. Along this line, the development of new beverages using alternative sweeteners could help with combatting the pathophysiological disorders associated to the consumption of sugar. To provide evidence on this issue, in the present work, the bioavailability of anthocyanins was evaluated after the acute ingestion of a new maqui-citrus-based functional beverage rich in polyphenols, and supplemented with a range of sweeteners including sucrose (natural high caloric), stevia (natural non-caloric), and sucralose (artificial non-caloric), as an approach that would allow reducing the intake of sugars while providing bioactive phenolic compounds (anthocyanins). This approach allowed the evaluation of the maximum absorption and the diversity of metabolites excreted through urine. The beverages created were ingested by volunteers (n = 20) and the resulting anthocyanin metabolites in their urine were analyzed by UHPLC-ESI-MS/MS. A total of 29 degradation metabolites were detected: Caffeic acid, catechol, 3,4-dihidroxifenilacetic acid, hippuric acid, trans-ferulic acid, 2,4,6-trihydroxybenzaldehyde, trans-isoferulic acid, and vanillic acid derivatives, where peak concentrations were attained at 3.5 h after beverage intake. Sucralose was the sweetener that provided a higher bioavailability for most compounds, followed by stevia. Sucrose did not provide a remarkably higher bioavailability of any compounds in comparison with sucralose or stevia. The results propose two sweetener alternatives (sucralose and stevia) to sucrose, an overused high calorie sweetener that promotes some metabolic diseases.

Drought stress is the most important stress factor for plants, being the main cause of agricultural crop loss in the world. Plants have developed complex mechanisms for preventing water loss and oxidative stress such as synthesis of abscisic acid (ABA) and non-enzymatic antioxidant compounds such as anthocyanins, which might help plants to cope with abiotic stress as antioxidants and for scavenging reactive oxygen species. A. chilensis (Mol.) is a pioneer species, colonizing and growing on stressed and disturbed environments. In this research, an integrated analysis of secondary metabolism in Aristotelia chilensis was done to relate ABA effects on anthocyanins biosynthesis, by comparing between young and fully-expanded leaves under drought stress. Plants were subjected to drought stress for 20 days, and physiological, biochemical, and molecular analyses were performed. The relative growth rate and plant water status were reduced in stressed plants, with young leaves significantly more affected than fully-expanded leaves beginning from the 5th day of drought stress. A. chilensis plants increased their ABA and total anthocyanin content and showed upregulation of gene expression when they were subjected to severe drought (day 20), with these effects being higher in fully-expanded leaves. Multivariate analysis indicated a significant positive correlation between transcript levels for NCED1 (9-cis-epoxycarotenoid dioxygenase) and UFGT (UDP glucose: flavonoid-3-O-glucosyltransferase) with ABA and total anthocyanin, respectively. Thus, this research provides a more comprehensive analysis of the mechanisms that allow plants to cope with drought stress. This is highlighted by the differences between young and fully-expanded leaves, showing different sensibility to stress due to their ability to synthesize anthocyanins. In addition, this ability to synthesize different and high amounts of anthocyanins could be related to higher NCED1 and MYB expression and ABA levels, enhancing drought stress tolerance. [ABSTRACT FROM AUTHOR]

To assess short-term efficacy and safety of a multicomponent nutraceutical (MCN) on dysmetabolism and oxidative stress, a pilot prospective observational study was performed on 21 individuals (12 men and 9 women) who took, for 60 days, 2 tablets per day of an MCN based on antioxidants andmetabolism regulators: hydroxytyrosol (15 mg), maqui (300 mg), amla (200 mg), monacolin K (10 mg), berberine (245 mg), astaxanthin (0.5 mg), coenzyme Q10 (100 mg), and folic acid (200 mcg). On day 0 (T0) and day 60 (T60), all participants underwent laboratory tests related to lipid profile, carbohydrate metabolism, oxidative stress, and cellular inflammation. Statistical analysis was applied to the resulting data. A significant improvement of most atherogenesis and oxidative stress biomarkers was recorded (mean figure at T0 and T60, p value): total cholesterol 243.50/194.83 mg/dl, p=.0002; low-density lipoproteins 174.50/124.58 mg/dl, p=.0001; glycemia 96.25/88.50 mg/dl, p=.035; total free radicals 306.44/280.93 U.Carr., p = .036; serum antioxidant capacity 2103.00/2246.06 umol/l, p = .0042; oxidized cholesterol 680.33/597.25 uEq/l, p = .0511. Insulinemia, microalbuminuria, high-density lipoproteins, C-reactive protein, and triglycerides had no statistically significant variation. Body weight and systo-diastolic pressure showed no significant change from T0 to T60. No relevant side effects were reported. The investigated MCN (Eonlipid), based on polyphenols, significantly improved the oxidative stress parameters and decreased themajority of atherogenesis parameters at short term. No significant side effects were reported. Further placebo-controlled studies should possibly corroborate the promising results of this pilot study. [ABSTRACT FROM AUTHOR]

[Display omitted] • Fermentation of maqui juice with kombucha consortium of different ages enhances its co-pigmentation effect. • Sucrose concentration influences anthocyanin co-pigmentation in kombucha analogs of maqui juice. • Fermented beverages rich in stable anthocyanins favor antioxidant responses and inhibit digestive enzymes. The fermentation of maqui juice (MJ), by incorporating kombucha as a starter culture, produces beverages with variable and stable anthocyanin contents. The metabolic effect of kombucha starter cultures obtained at different fermentation times was explored on the anthocyanin stability of maqui (Aristotelia chilensis (Mol.) Stuntz) juice supplemented at different concentrations of sucrose and fermented at different times. The stability of anthocyanins was associated with the levels of catechin detected in the fermentation system. This study concludes that the fermentation of MJ with sucrose (10%) and kombucha consortium of 7-days old, promotes the release and accumulation of phenolic compounds that act as co-pigments, with the best response in quality parameters of beverages such as color intensity, tone, hyperchromic effect, and a significant bathochromic shift. Finally, the additive effect of phenolic constituents with stable anthocyanins, confers to kombucha analogs an outstanding antioxidant quality and inhibitory effects on key enzymes in digestive processes. [ABSTRACT FROM AUTHOR]

The principle threats for sustainable production of wild collected medicinal plants are related to ecological factors, such as endemism, and botanical factors critical for survival, such as the collection of roots or barks or slow growing species. The sustainable way to produce raw material on a large scale would be species specific management of the wild resources that guarantees conservation of biodiversity, or bringing the species under cultivation. A checklist proposed by WHO, UICN and WWF (1993) indicates that domestication of any medicinal plant concerns plant selection and breeding, studies about propagation, cultivation techniques, plant protection, time of harvest, among others. The different domestication steps are illustrated for the Chilean maqui (Aristotelia chilensis), a wild tree whose fruits are demanded in increasing volumes by the international market because of its high antioxidant capacity. High yielding plants with good fruit quality have been selected from wild populations and accessions have been cultivated under different environmental conditions to select the most suitable genotypes for the establishment of commercial orchards.

Con el fin de mejorar la germinación de semillas de Aristotelia chilensis, se establecieron dos ensayos in vitro. En el primero, se realizó escarificación y se comparó con un testigo. En el segundo, se aplicó estratificación fría por diferentes períodos de tiempo (2, 4, 6 y 8 semanas a 4 °C en oscuridad), más un testigo sin estratificar. Las semillas fueron germinadas en placas con agua y agar (AA) e incubadas por 60 días en cámara de cultivo a 25 ± 2 °C, con tubos fluorescentes de luz fría blanca bajo un fotoperíodo de 16 h luz, 8 h de oscuridad y una intensidad lumínica de 50 μmol m-2 s-1. En todos los tratamientos se logró valores promedios de germinación superiores a los testigos. La mayor germinación se obtuvo con escarificación (Gmáx, 92%), no obstante la mayor velocidad inicial de germinación se registró con la estratificación por ochomáx semanas, alcanzando el menor tiempo medio de germinación (TM, 18 días). Los resultados demuestran que la semilla de A. chilensis presenta dormancia primaria de nivel moderado tanto exógena como endógena.

In recent years, there has been an increasing interest in studying food and its derived ingredients that can provide beneficial effects for human health. These studies are helping to understand the bases of the ancestral use of several natural products, including native fruits as functional foods. As a result, the polyphenol profile and the antioxidant capacity of the extracts obtained from different Patagonian native berries have been described. This review aims to provide valuable information regarding fruit quality, its particular compound profile, and the feasibility of producing functional foods for human consumption to prevent disorders such as metabolic syndrome and cardiovascular diseases. We also discuss attempts concerning the domestication of these species and generating knowledge that strengthens their potential as traditional fruits in the food market and as a natural heritage for future generations. Finally, additional efforts are still necessary to fully understand the potential beneficial effects of the consumption of these berries on human health, the application of suitable technology for postharvest improvement, and the generation of successfully processed foods derived from Patagonian berries.

Aristotelia chilensis (Molina) Stuntz (Elaeocarpaceae) also known as 'maqui' is a dioecious tree species native to Chile and neighbouring zones of Argentina. Its fruit is collected from the wild by locals for consumption, and recently, as a raw material for industrial processing because of its high antioxidant capacity. As a consequence of its increasing demand, sustainable production is required. To study intraspecific diversity patterns we therefore analysed 58 accessions, growing in the Experimental Station of Universidad de Talca and originating from eight wild populations, using AFLPs and chloroplast microsatellites. Only 5% of the variability could be attributed to the provenance, whereas 95% was found between individuals of the same population. A significant correlation between genetic differentiation and geographic distances was detected (r = 0.51). Bayesian analysis revealed four main genetic groups, which are not correlated to the provenances. Two chloroplast microsatellite primers revealed two haplotypes of which one was detected in individuals from all the populations, whereas the other was only present in the two northernmost populations. The genetic variability found for this species provides an excellent basis for further selection and breeding. [ABSTRACT FROM AUTHOR]

Maqui ( Aristotelia chilensis [Molina] Stuntz) berry dehydration, drying kinetics (40-80°C), and drying temperature effects on texture profile, rehydration properties, dietary fiber, and sugar contents were evaluated. The Midilli-Kucuk model presented the best fitting to the drying curves. The effective diffusion coefficients varied from 0.34 × 10−10 m2 s−1 at 40°C to 2.14 × 10−10 m2 s−1 at 80°C. The texture profile analysis showed a slight difference in some parameters (springiness, gumminess cohesiveness, and chewiness). The WHC parameters had no significant differences in the range 50-80°C. The insoluble dietary fiber content was greater than the soluble one in all the samples. Also, the dietary fiber, fructose, and glucose contents increased in value with an increase in temperature. The results could be used to estimate the best drying conditions for producing a dehydrated maqui product. Practical application This native berry is categorized as a 'superfruit' due to its great number of wholesome properties. However, it is highly perishable and is primarily marketed as a powder since it is stable after drying. This study is the first one that provides results on both quality changes and energy consumption of the drying process. This information, here, can help engineers and marketers to determine the best drying conditions for maqui berries so that they retain their antioxidant properties and dietary fiber contents as well as maintain proper rehydration and texture properties, all of them interesting for to functional food marketing. [ABSTRACT FROM AUTHOR]

The optimum conditions for the antioxidant extraction from maqui berry were determined using a response surface methodology. A three level D-optimal design was used to investigate the effects of three independent variables namely, solvent type (methanol, acetone and ethanol), solvent concentration and extraction time over total antioxidant capacity by using the oxygen radical absorbance capacity (ORAC) method. The D-optimal design considered 42 experiments including 10 central point replicates. A second-order polynomial model showed that more than 89% of the variation is explained with a satisfactory prediction (78%). ORAC values are higher when acetone was used as a solvent at lower concentrations, and the extraction time range studied showed no significant influence on ORAC values. The optimal conditions for antioxidant extraction obtained were 29% of acetone for 159 min under agitation. From the results obtained it can be concluded that the given predictive model describes an antioxidant extraction process from maqui berry. [ABSTRACT FROM AUTHOR]

Healthy ageing involves the interaction between genes, the environment, and lifestyle factors, particularly diet. Diet-regulated genes play a crucial role in the onset and progression of several chronic disorders including skin diseases and dietary interventions can be used to mitigate these diseases but also to maintain health. The nutrient regulation of key genes involved in ageing may prevent oxidation and inflammation, reducing cellular damages to proteins, membranes, and mitochondria. In recent years there has been a growing interest, supported by a large number of experimental and epidemiological studies, about the beneficial effects of some commonly used food-derived products, including macro-and micronutrients and dietary phytochemicals. In particular, fruits, spices and herbs often contain active phenolic substances endowed with potent antioxidant and chemo-preventive properties. Among polyphenols, anthocyanins, a subfamily belonging to the flavonoid group, have shown to modulate a variety of biochemical/signalling pathways involved in promoting organism physiology and general health status, including vasculo-protective effects, cognitive process enhancement, anti-cancer activity and skin photo-protection. Among the anthocyanin species, delphinidin [2-(3,4,5-trihydroxyphenyl) chromenylium-3,5,7-triol] represent the most potent antioxidant. The richest known natural source of delphinidin is the maqui berry (Aristotelia chilensis), a super-fruit indigenous to Chile. We have recently performed a randomized double-blind nutritional trial, and demonstrated the in vivo ability of maqui polyphenols to protect lipids from oxidative damages. This and other studies have begun to provide a basis for considering the use of maqui and delphinidin in the development of novel nutritional interventional strategies for health management and against specific age-associated diseases. In this review we will provide an overview of the current literature emphasizing antioxidant and anti-inflammatory pathways modulated by maqui berry and its polyphenolic components, mostly delphinidin. Moreover, we will focus on experimental studies showing that delphinidin of maqui may have a positive impact on skin health. [ABSTRACT FROM AUTHOR]

The optimum conditions for the antioxidant extraction from maqui berry were determined using a response surface methodology. A three level D-optimal design was used to investigate the effects of three independent variables namely, solvent type (methanol, acetone and ethanol), solvent concentration and extraction time over total antioxidant capacity by using the oxygen radical absorbance capacity (ORAC) method. The D-optimal design considered 42 experiments including 10 central point replicates. A second-order polynomial model showed that more than 89% of the variation is explained with a satisfactory prediction (78%). ORAC values are higher when acetone was used as a solvent at lower concentrations, and the extraction time range studied showed no significant influence on ORAC values. The optimal conditions for antioxidant extraction obtained were 29% of acetone for 159 min under agitation. From the results obtained it can be concluded that the given predictive model describes an antioxidant extraction process from maqui berry.

Measuring the level of inflammation with an innovative approach (with blood analyses to evaluate the action of cytokines such as B-cell activating factor [BAFF], platelet activating factor [PAF], interleukin-6 [IL-6], sirtuin) allows you to better understand the body's language. The body recognizes foods and food antigens through a mapping performed by means of immunoglobulin G (IgG). Recent studies have shown that high levels of IgG in a nutrient express the personal excess of that food in the daily nutrition. Food is the most important source of hidden inflammation but is also the main tool for regaining wellness. Europeans have reactions to milk, yeast, and gluten, whereas the Japanese have reactions to rice and soybeans. Scientific knowledge of inflammatory mediators allows the discovery of new drugs, new supplements, and new plant compounds (Resveratrol, Maqui, oil of Perilla) that are able to reduce inflammation and support well-being. The interaction between the environment, nutrition, and knowledge of inflammation is an evolution and at the same time a revolution to gain and maintain health. [ABSTRACT FROM AUTHOR]

Apple and quince leathers were enriched by maqui ( Aristotelia chilensis Mol. Stuntz) extract to enhance their antioxidant capacity naturally and to develop a new functional snack for new markets. Fruit puree was dehydrated at 60 °C for 20 h and subjected to accelerated storage. Soluble solids, acidity, pH, water activity (aw), total phenolic (TP), antioxidant activity (AOA) and capacity (ORAC), and color change (browning index, BI) were measured in leathers and raw materials. An untrained panel was used to assess consumer acceptability. Leathers had intermediate aw (0.56–0.69), a moisture content of 17 kg water/100 kg, and 70° Brix. The addition of maqui changed the leather's color and BI. TP and AOA were higher (p ≤ 0.05) in quince formulations. Apple puree fortified with maqui showed 40% and 45% increased in TP and AOA, respectively. Antioxidants decreased up to 59% in leathers compared to fruit puree. TP slightly decreased and the AOA remained unchanged during storage. The BI increased 0.1 and 0.3 units per day in apple leathers with and without maqui, respectively, while the AOA did at −0.002 and 0.008 mg CAE/100 g per day. Untrained panelists preferred the apple to quince or apple with maqui leathers. [ABSTRACT FROM AUTHOR]

The natural compound hobartine {systematic name: (1 R)-3-[(1 S,5 S)-(4,4,8-trimethylbicyclo[3.3.1]non-7-en-2-yl)methyl]-2,3-dihydro-1 H-indole}, C20H26N2, (I), is an indole alkaloid isolated from Aristotelia chilensis as part of a study of secondary metabolites from Chilean flora. The colourless compound has a tetracyclic structure closely related to the strongly coloured polymorphic structures discussed in Paz et al. [ Acta Cryst. (2013), C 69, 1509-1512] and Watson et al. [ Acta Cryst. (1989), C 45, 1322-1324]. The main differences reside in the absence of a keto group in (I) compared with the previous structures, as well as an endo double bond in (I) contrasting with the exo double bond found in the previous structures. The supramolecular structure of (I) in strongly related to the twofold screw axis, around which isolated chains build up, internally linked by an N-H...N hydrogen bond which is the only significant intermolecular interaction present in the structure. [ABSTRACT FROM AUTHOR]

Objectives: To assess the immediate/short-term outcomes of a maqui berry-based nutraceutical (MBN) supplementation on the autonomic nervous system (ANS), resilience level, metabolism and body composition., Methods: A randomized controlled single-blind study was performed on healthy subjects. Throughout 30 days, one group took 1,000 mg/day of an MBN (Maqui 500 ® ), the control group had no supplementation. On day 0 (T0) and 30 (T3) all subjects performed blood tests, bioimpedance spectroscopy and questionnaires for resilience, perceived stress and short-form 12 (SF12). At T0, 75' after T0 (T1), on day 7 and at T3 the subjects underwent biometric parameter measurement and heart rate variability (HRV) test to investigate psycho-physical resilience., Results: Fifteen subjects per group were included; abnormal seasonal high temperatures altered individuals' lifestyle and nutrition, influencing the trial's outcomes. Biometric parameters, blood pressure, oxygen saturation and blood tests did not differ between T0 and T3 in both groups. In the MBN group the HRV analysis showed a significant increase of ANS coordination (p=0.05), parasympathetic activity at 75', very low frequencies and total power at T3, whereas these parameters decreased in the control group. SF12 mental score improved in the maqui group (p=0.02); the questionnaire-based outcomes showed no further variations. In the control subjects bioimpedance showed an increase of resistance and fat mass, with decreased total body water and lean mass (p=n.s.)., Conclusions: The maqui-based nutraceutical improved HRV, namely ANS activation/coordination, and SF12 mental component. Blood tests and bioimpedance/biometric parameters mildly varied. The elapsed hot weather likely biased many investigated variables., (© 2022 Walter de Gruyter GmbH, Berlin/Boston.)

Drought stress reduces plant growth and crop yields. Plants activate defense mechanisms, such as stomatal closure, phenolic compounds biosynthesis, compatible solutes, protein, amino acids, and abscisic acid to cope with drought stress. Most studies on drought stress effects have been focused only on fully expanded leaves, while information on young leaves is scarce. In this study, we investigated the differential photosynthetic performance as well as the primary and secondary metabolic responses in young and fully expanded leaves of Aristotelia chilensis plants subjected to drought stress. We found that drought stress negatively affected net CO 2 assimilation (A N), stomatal conductance (g s), and transpiration (E) more drastically in young leaves than fully expanded leaves of A. chilensis plants. On the other hand, young leaves accumulated higher total protein than fully expanded leaves of plants at day 10 of drought stress. Likewise, the total amino acid was increased in young leaves of stressed plants on days 5, 10, and 20, showing higher values at the end of the experiment. Starch was reduced in both young and fully expanded leaves of drought-stressed plants, while sucrose and fructose levels increased around 2-fold in young leaves at days 10 and 20 of drought stress. We observed that leucine and valine increased in young leaves at days 10 and 20 of drought stress, while in contrast, no changes were observed in the fully expanded leaves. PCA analysis clearly separated the young from fully expanded leaves at 10 and 20 days under drought stress, where anthocyanins and related genes (UFGT and NCED) were stimulated in fully expanded leaves, while in young leaves was amino acids, sucrose, and fructose. Our results demonstrate that young leaves stimulate branched-chain amino acid and phenylalanine accumulation, while fully expanded leaves stimulate anthocyanins and related genes, showing different physiological, biochemical, and molecular mechanisms to respond to drought stress. [Display omitted] • Drought stress negatively affected gas-exchange in young than fully expanded leaves of Aristotelia chilensis plants. • Young leaves accumulated higher amount of total protein, and total amino acid levels than fully expanded leaves. • Sucrose and fructose levels increased around 2-fold in young leaves under drought stress. • Young leaves stimulate branched-chain amino acid and phenylalanine accumulation. • Fully expanded leaves stimulate secondary metabolites biosynthesis under drought stress. [ABSTRACT FROM AUTHOR]

The title compound [systematic name: (4,4-dimethyl-8-methylene-3-azabicyclo[3.3.1]non-2-en-2-yl)(1 H-indol-3-yl)methanone], C20H22N2O, (II), was obtained from mother liquors extracted from Aristotelia chilensis (commonly known as maqui), a native Chilean tree. The compound is a polymorphic form of that obtained from the same source and reported by Watson, Nagl, Silva, Cespedes & Jakupovic [ Acta Cryst. (1989), C 45, 1322-1324], (I a). The molecule consists of an indolyl ketone fragment and a nested three-ring system, with both groups linked by a C-C bridge. Comparison of both forms shows that they do not differ in their gross features but in the relative orientation of the two ring systems, due to different rotations around the bridge, as measured by the O=C-C=N torsion angle [130.0 (7)° in (I a) and 161.6 (2)° in (II)]. The resulting slight conformational differences are reflected in a number of intramolecular contacts being observed in (II) but not in (I a). Regarding intermolecular interactions, both forms share a similar N-H...O synthon but with differing hydrogen-bonding strength, leading in both cases to C(6) catemers with different chain motifs. There are marked differences between the two forms regarding colour and the (de)localization of a double bond, which allows speculation about the possible existence of different variants of this type of molecule. [ABSTRACT FROM AUTHOR]

Microencapsulation technology is an alternative to stabilize stress factors and protect food ingredients or additives, which include environmentally sensitive bioactive principles in protective matrices to increase their functionality and life span. The objective of this research was to study conditions to obtain microcapsules with antioxidant capacity from a maqui (Aristotelia chilensis [Molina] Stuntz, Elaeocarpaceae) leaf extract by emulsification and subsequent retention after microencapsulation. Microcapsules were produced by water-in-oil emulsion (W/O) using a phase of the aqueous maqui leaf extract and gum arabic, and a liquid vaseline phase. Maqui leaf extract antioxidant capacity was 99.66% compared with the aqueous phase of the emulsion at 94.38 and 93.06% for 5 % and 15 % gum arabic, respectively. The mean yield of maqui leaf extract microencapsulation with 5% gum arabic varied between 38 and 48%, whereas with 15% gum arabic it was 39%. Once the antioxidant microcapsules were formed, mean extract antioxidant capacity ranged between 30 and 35%. Both yields responded similarly to changes in gum arabic concentrations (5% and 15%) in the aqueous phase of the emulsion; 5% concentration produced a microcapsule size from 1.0 to 10 µm. Maqui leaf extracts with high phenolic compound levels, which can be stabilized and protected by the microencapsulation process, produce new natural preservative systems as compared with their synthetic counterparts. [ABSTRACT FROM AUTHOR]

Alzheimer's disease (AD) is characterized by the presence of different types of extracellular and neurotoxic aggregates of amyloid-β (Aβ). Recently, bioactive compounds extracted from natural sources showing neuroprotective properties have become of interest in brain neurodegeneration. We have purified, characterized, and evaluated the protective potential of one extract enriched in polyphenols obtained from Aristotelia chilensis (MQ), a Chilean berry fruit, in neuronal models of AD induced by soluble oligomers of Aβ1-40. For example, using primary hippocampal cultures from rats (E18), we observed neuroprotection when the neurons were co-incubated with Aβ (0.5 μM) plus MQ for 24 h (Aβ = 23 ± 2%; Aβ + MQ = 3 ± 1%; n = 3). In parallel, co-incubation of Aβ with MQ recovered the frequency of Ca2+ transient oscillations when compared to neurons treated with Aβ alone (Aβ = 72 ± 3%; Aβ + MQ = 86 ± 2%; n = 5), correlating with the changes observed in spontaneous synaptic activity. Additionally, MAP-2 immunostaining showed a preservation of the dendritic tree, suggesting that the toxic effect of Aβ is prevented in the presence of MQ. A new complex mechanism is proposed by which MQ induces neuroprotective effects including antioxidant properties, modulation of cell survival pathways, and/or direct interaction with the Aβ aggregates. Our results suggest that MQ induces changes in the aggregation kinetics of Aβ producing variations in the nucleation phase (Aβ: k1 = 2.7 ± 0.4 × 10-3 s-1 MQ: k1 = 8.3 ± 0.6 × 10-3 s-1) and altering Thioflavin T insertion in β-sheets. In conclusion, MQ induces a potent neuroprotection by direct interaction with the Aβ aggregates, generating far less toxic species and in this way protecting the neuronal network. [ABSTRACT FROM AUTHOR]

The principle threats for sustainable production of wild collected medicinal plants are related to ecological factors, such as endemism, and botanical factors critical for survival, such as the collection of roots or barks or slow growing species. The sustainable way to produce raw material on a large scale would be species specific management of the wild resources that guarantees conservation of biodiversity, or bringing the species under cultivation. A checklist proposed by WHO, UICN and WWF (1993) indicates that domestication of any medicinal plant concerns plant selection and breeding, studies about propagation, cultivation techniques, plant protection, time of harvest, among others. The different domestication steps are illustrated for the Chilean maqui (Aristotelia chilensis), a wild tree whose fruits are demanded in increasing volumes by the international market because of its high antioxidant capacity. High yielding plants with good fruit quality have been selected from wild populations and accessions have been cultivated under different environmental conditions to select the most suitable genotypes for the establishment of commercial orchards. [ABSTRACT FROM AUTHOR]

Glucagon-like peptide (GLP)-1 and the glucose-dependent insulinotropic peptide (GIP) are incretin hormones that regulate the nutrient-stimulated insulin secretion from pancreatic β-cells. Their low plasma concentrations and rapid clearance pose certain methodological challenges for their detection and quantification. The currently available immunomediated techniques to monitor these hormones overestimate, to some extent, their actual concentration. Hence, the present study is aimed at developing a robust and reliable methodology for the identification and quantification of active and inactive forms of the incretins GLP-1 and GIP, in human plasma, by UHPLC-ESI-QqQ-MS/MS. A comparative study of different SPE cartridges was carried out, being identified OASIS HLB as the most efficient one, with recoveries up to 80%. The method provides adequate linearity, from 4.88 to 1250 nM, and low intervals of LOD and LOQ for each analyte (ranges from 0.01 to 3.42 nM and from 0.10 to 34.17 nM, respectively). The methodology described was validated upon a clinical trial with overweight subjects (n = 20) (ClinicalTrials.gov NCT 04016337), showing the capacity of the newly developed methodology to detect the augment of the plasma concentration of both GLP-1 7-36 and GLP-1 9-36 between 30 and 60 min after the consumption of a sucrose sweetened fruit-based beverage, while the plasma concentration of GIP remained in levels lower than the LOQ. The proposed methodology provides further insights into the mechanisms of action of bioactive compounds and food components in the frame of the glycemic control and would contribute to the assessment of the efficacy of antidiabetic drugs. [Display omitted] • Validation of a UPLC-MS/MS method for determination of GIP and GLP-1 in human plasma. • Selective capacity to distinguish active and inactive forms of the peptide hormones. • Simple SPE with good recoveries up to 80% with OASIS HLB cartriges • No application of digestion or immunoaffinity steps. • Good performance in terms of precision, accuracy, sensitivity, linearity, recovery. [ABSTRACT FROM AUTHOR]

Maqui (Aristotelia chilensis (Mol.) Stuntz) is a Chilean berry rich in antioxidants, which are mostly found in the pulp and skin of the fruit. The objective was to evaluate the cryoconcentration process by centrifugation–filtration as a simultaneous, efficient, and innovative method to increase the content of thermosensitive bioactive compounds of aqueous maqui extract. Cryoconcentration separated the concentrated solute from the aqueous maqui extract with an efficiency of more than 95%; it increased the content of total polyphenols and total anthocyanins and antioxidant capacity by 280%, 573%, and 226%, respectively. Although the concentrates obtained by evaporation at 50, 70, and 80 °C increased the content of bioactive compounds, they did so in a lower percentage than the cryoconcentrate. Furthermore, cyanidin 3,5-diglucoside was degraded at 70 and 80 °C. In conclusion, cryoconcentration by centrifugation–filtration as a simultaneous process efficiently separates the solutes from the frozen matrix of aqueous maqui extract, and it maintains and increases the contents of polyphenols and anthocyanins and antioxidant capacity. This method is recommended for concentrating natural berry extracts with thermosensitive compounds. [ABSTRACT FROM AUTHOR]

Abstract: Aristotelia chilensis (Mol.) Stuntz (A. chilensis), also known as maqui, is a plant of the Elaeocarpaceae family that grows in central and southern Chile as well as southwestern Argentina. Infusions of its leaves have long been used in the traditional native herbal medicine to treat different ailments. Phytochemical studies of the plant''s chemical composition of the plant indicate the presence of indolic alkaloids, flavonoids, cianidine glucosides, delfidine, malvidine, petunidine, cumarines and triterpenes. These compounds, particularly the flavonoids, have antioxidant properties. In order to evaluate the mechanisms of its toxicity and their antioxidant properties, the leaves’ aqueous extracts were induced to interact with human red cells, their isolated unsealed membranes (IUM), and molecular models of the human erythrocyte membrane. These consisted of multibilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipids classes located in the outer and inner monolayers of the human erythrocyte membrane, and large unilamellar vesicles (LUV) of DMPC. The capacity of A. chilensis aqueous extracts to perturb the bilayer structure of DMPC and DMPE was evaluated by X-ray diffraction, DMPC LUV and IUM were studied by fluorescence spectroscopy, and intact human erythrocytes were observed by scanning electron microscopy (SEM). Results of the present study indicate that aqueous extracts of A. chilensis induced an alteration of human erythrocyte morphology from the normal discoid shape to an echinocytic form, changes that are explained in terms of the extract interaction with the membrane''s outer phospholipid monolayer. [Copyright &y& Elsevier]

This article deals with the visual quality of Mediterranean vegetation groups in northern Israel, the public's preference of these groups as a visual resource, and the policy options for their management. The study is based on a sample of 44 Mediterranean vegetation groups and three population groups of local residents, who were interviewed using a questionnaire and photographs of the vegetation groups. The results of the research showed that plant classification methods based on flora composition, habitat, and external appearance were found to be suitable for visual plant classification and for the evaluation of visual preference of vegetation groups by the interviewed public. The vegetation groups of planted pine forests and olive groves, characterizing a cultured vegetation landscape, were preferred over typical Mediterranean landscapes such as scrub and grassed scrub. The researchers noted a marked difference between the two products of vegetation management policy, one that proposes the conservation and restoration of the variety of native Mediterranean vegetation landscape, and a second that advanced the development of the cultured landscape of planted olive groves and pines forests, which were highly preferred by the public. The authors suggested the development of an integrated vegetation management policy that would combine both needs and thus reduce the gap between the policy proposed by planners and the local population's visual preference. [ABSTRACT FROM AUTHOR]