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Simple Summary: Dairy goats, as an important economic livestock for the milk supply in dairy products, have been raised worldwide. However, with the intensive management of dairy goat breeding, the disease problems are becoming increasingly serious, among which Corynebacterium pseudotuberculosis has been spreading in dairy goat breeding in China. In this study, we analyzed the transcriptome and proteomics of dairy goats infected with Corynebacterium pseudotuberculosis at 72 h and 144 h. Through a comparative correlation analysis of the data, we hope to find the pathogenic mechanism and provide a theoretical basis for the disease problems in dairy goat breeding. Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a zoonotic chronic infectious disease. It mainly occurs in dairy goats reared in herds, and once it invades the dairy goats, it is difficult to completely remove it, causing great harm to the development of the sheep industry. This study mainly was based on TMT-based quantitative proteomics and RNA-seq methods to measure the spleen samples of infected dairy goats at different time periods. Nine four-month-old dairy goats were divided into three groups, with three goats in each group. The dairy goats in the first group (NC group) were inoculated with 1.0 mL of sterilized normal saline subcutaneously, and the second (72 h group) and third groups (144 h group) were inoculated with 1.0 mL of 1 × 107 cfu/mL bacterial solution subcutaneously in the neck. Significant changes in the protein and mRNA expression were observed in different infection and control groups. In the 72 h group, 85 genes with differential genes and proteins were up-regulated and 91 genes were down-regulated in this study. In the 144 h group, 38 genes with differential genes and proteins were up-regulated and 51 genes were down-regulated. It was found that 21 differentially expressed genes and proteins were co-up-regulated in the two groups. There were 20 differentially expressed genes and proteins which were co-down-regulated in both groups. The 72 h group were mainly enriched in protein processing in the endoplasmic reticulum, lysosome, amino sugar and nucleotide sugar metabolism and the estrogen signaling pathway. In the 144 h group, they were protein processing in the endoplasmic reticulum pathway which was enriched by mRNA–proteins pairs co-upregulated by the five pairs. The combined transcriptomic and proteomic analyses were performed to provide insights into the effects of C. pseudotuberculosis through several regulatory features and pathways. We found that in the early stage of infection (72 h), the co-upregulated gene–protein pairs were enriched in multiple pathways, which jointly defended against a bacterial invasion. However, in the later stages of infection (144 h), when the disease stabilizes, a few co-upregulated gene–protein pairs played a role in protein processing in the endoplasmic reticulum pathway. In addition, the mRNA and protein expressions of dairy goats infected with the bacteria at different periods of time indicated the adaptability of dairy goats to the bacteria. At the same time, it guides us to carry out a corresponding treatment and feeding management for dairy goats according to different periods of time. [ABSTRACT FROM AUTHOR]

Self-compacting concrete (SCC) has been widely used in building structures. However, previous research focused only on the mechanical properties and working properties of SCC at room temperature. Thus, there is limited research on the change of compressive strength of SCC after a fire. This paper aims to investigate the compressive properties of SCC after being cooled from high temperatures. The SCC specimens were firstly heated to a target temperature of 100–700 °C and were then cooled to ambient temperatures by water or in air. The heating and cooling damage to the SCC specimens was assessed by the mass loss and the ultrasonic pulse velocity (UPV) separately. Afterward, the axial compression tests were carried out to investigate the compressive properties of the fire-affected SCC specimens under uniaxial compression. The residual mass, UPV, stress–strain curves, post-fire failure characteristics, and compressive strengths of the SCC specimens were discussed in detail. The mass loss of the SCC specimens showed an obvious increase with the rising temperatures, while the UPV exhibited a converse pattern. The mass loss of the SCC specimens after being naturally cooled increased more significantly, while the two cooling methods used in this experiment had little effect on the UPV. When the SCC specimens were cooled from 100 °C, the compressive strength of the SCC specimens cooled in air or water dropped by 32.54% and 35.15%, respectively. However, while the heating temperature rose to 700 °C, the compressive strengths of the SCC specimens cooled in air or water dropped sharply by 72.98% and 86.51%, respectively. Finally, an improved mathematical model for SCC after cooling from high temperatures was proposed based on Jones and Nelson's equation. This improved material model matched the experimental results well, which demonstrates that the proposed constitutive model can provide better predictions for the SCC structures after a fire. [ABSTRACT FROM AUTHOR]

To not only achieve the goal of urban cultural construction but also save the cost of urban sculpture space design, EC (edge computing) is combined with urban sculpture space design and planning first. Then it briefly discusses the service category, system architecture, advantages, and characteristics of urban sculpture, as well as the key points and difficulties of its construction, and the layered architecture of EC for urban sculpture spaces is proposed. Secondly, the cloud edge combination technology is adopted, and the urban sculpture is used as a specific function of the edge system node to conduct an in-depth analysis to build an urban sculpture safety supervision system architecture platform. Finally, the actual energy required for implementation is predicted and evaluated, the specific monitoring system coverage is set up, and some equations are made for calculating the energy consumption of the monitored machines according to the number of devices and route planning required by the urban sculpture safety supervision system. An optimization algorithm for energy consumption is proposed based on reinforcement learning and compared with the three control groups. The results show that when the seven monitoring devices cover detection points less than 800, the required energy consumption increases linearly. When the detection devices cover more than 800 detection points, the required energy consumption is stable and varies from 10000 to 12000; that is, when the number of monitoring devices is 7, the optimal number of monitoring points is about 800. When the number of detection points is fixed, increasing the number of monitoring devices in a small range can reduce the total energy consumption. The optimization algorithm based on the reinforcement learning proposal can obtain an approximate optimal solution. The research results show that the combination of edge computing and urban sculpture can expand the function of urban sculpture and make it serve people better. [ABSTRACT FROM AUTHOR]

Pou2F3 (POU class 2 homeobox 3) is found to be ubiquitously expressed in multiple epidermal layer cells to mediating proliferation. Although some POU factors exert a crucial regulation in mammary epithelial cells (MECs), the biological function of Pou2F3 is unclear. In this study, we aimed to investigate the endogenous potential effects of Pou2F3 on the proliferation and the roles of PI3K/AKT/mTOR signaling pathway in MECs. We used small interfering RNA to silence Pou2F3 expression. The interfering efficiency of Pou2F3 was confirmed by using RT-qPCR and Western blot. The cell viability and proliferation were indicated by Cell Counting Kit-8 and EdU assays. Flow cytometry was performed to evaluate the cell apoptosis in MECs. These results demonstrated that Pou2F3 potently suppressed the proliferation and induced the apoptosis of MECs. Consistently, the primary protein expressions of PI3K/AKT/mTOR signaling pathway were examined by Western blot. Pou2F3 silencing significantly increased the phosphorylation of PI3K, AKT and mTOR expressions. Moreover, Pou2F3 silencing reduced the ratio of BCL-2/BAX protein expression. Our findings show that Pou2F3 silencing can induce the proliferation of MECs and decrease the cell apoptosis, which suggest that Pou2F3 may serve as a potential upstream regulator of PI3K/AKT/mTOR signaling pathway in MECs. [ABSTRACT FROM AUTHOR]

Goat milk in some cases is less allergenic than cow milk, therefore, more people drink goat milk in the world, so it is necessary for us to improve the yield and quality of goat milk. Previous studies have shown that some genes are closely related to lactation. Ovarian cancer G protein-coupled 1 (OGR1) is a G protein-coupled receptor discovered recently. OGR1 is widely found in various tissues of organisms and is involved in cell skeleton reorganization, carcinogenesis, cell proliferation, and apoptosis by regulating multiple signaling pathways in cells. However, the modulating effect of OGR1 in lactation is still unknown. Therefore, the objective of this study is to investigate the function of OGR1 in goat mammary epithelial cells (GMECs). Flow cytometry, CCK8, EDU, enzyme-linked immunosorbent assay, and triglyceride test kit assays were performed and we found that OGR1 regulated Bcl-2/Bax ratio, Fas protein expression as well as the phosphorylation of AKT and mammalian target of rapamycin (mTOR). si-OGR1 could enhance the proliferation of GMECs by promoting G1/S phase progression and the synthesis of β-casein and triglyceride. By contrast, OGR1 repressed GMECs proliferation and down-regulated the synthesis of β-casein and triglyceride by blocking the PI3K/AKT/mTOR signaling pathway in GMECs. [ABSTRACT FROM AUTHOR]

Acridinium benzoate was developed as a unique ICT‐based fluorescent scaffold for both ratiometric and turn‐on fluorescence imaging through decaging of the phenolic hydroxyl groups. Two fluorescent probes, Acr1‐H2O2 and Acr1‐β‐gal, were developed for the fluorescence imaging of H2O2 and β‐galactosidase in vivo. [ABSTRACT FROM AUTHOR]

Introduction of a heteroatom into a fluorophore was carried out for coumarin through a replacement of its bridging oxygen atom with a silicon atom. The maximum-emission wavelength of Si-coumarin (SiC B) bathochromically shifted from 426 nm in cyclohexane to 626 nm in water. The adipogenic differentiation processes in mesenchymal stem cells were monitored using SiC B. [ABSTRACT FROM AUTHOR]

As a kind of medical service around people, community health care is closely related to peoples lives, and thus it has also been placed higher requirements. In the face of growing community medical needs, the construction and development of community medical Internet of things is imminent. Subsequently, massive multi-type of medical data which contain all kinds of user identity data, various types of vital signs data and other sensitive information are generated. Such a large scale of data in the transmission, storage and access process is facing the risk of data leakage. To effectively protect the privacy information of patients, an infrastructure framework for privacy protection of community medical Internet of things is proposed. It includes transmission protection based on multi-path asymmetric encryption fragment transmission mechanism, storage protection using distributed symmetric encryption cloud storage scheme and access control with identity authentication and dynamic access authorization. Through theoretical analysis and simulation experiments, it is proved that the community medical data can be effectively protected. [ABSTRACT FROM AUTHOR]

Based on the monitoring data of lightning activity in Huai'an from 2011 to 2014, the temporal and spatial distribution of lightning activity and lightning density were analyzed. The results show that positive and negative lightning activity in Huai'an had obvious seasonal variations and diurnal variations during 2011-2014. The month changes of positive and negative lightning activity had a single peak and valley, while the diurnal variations of both positive and negative lightning activity had double peaks and valleys. The high-value areas of ground flash density in Huai'an had obvious randomness. Seen from the spatial distribution of annual average of ground flash density, lightning activity was mainly distributed in Xuyi County, Jinhu County and Huai'an District. [ABSTRACT FROM AUTHOR]

On the basis of Internet of Things (IoT) technologies, Community Medical Internet of Things (CMIoT) is a new medical information system and generates massive multiple types of medical data which contain all kinds of user identity data, various types of medical data, and other sensitive information. To effectively protect users’ privacy, we propose a secure privacy data protection scheme including transmission protection and access control. For the uplink transmission data protection, bidirectional identity authentication and fragmented multipath data transmission are used, and for the downlink data protection, fine grained access control and dynamic authorization are used. Through theoretical analysis and experiment evaluation, it is proved that the community medical data can be effectively protected in the transmission and access process without high performance loss. [ABSTRACT FROM AUTHOR]

Simple Summary: Yield and quality of goat milk are important indexes for screening dairy goat breeds. Therefore, it is necessary for us to improve the yield and quality of goat milk. In this study, we demonstrated that circRNA8220/miR-8516/STC2 could promote the synthesis of β-casein and triglyceride through PI3K/AKT/mTOR pathway. In addition, we found that circRNA8220/miR-8516/STC2 also promote proliferation via Ras/MEK/ERK pathway in goat mammary epithelial cells (GMECs). These findings contribute to a better understanding of circRNA-controlled breast development and lactation mechanisms and provide new potential insights into the regulation of breast development and milk composition in dairy goats. Circular RNAs (circRNAs), which are considered a large class of endogenous noncoding RNAs, function as regulators in various biological procedures. In this study, the function and molecular mechanisms of circRNA8220 in goat mammary epithelial cells (GMECs) were explored. CircRNA8220 could spong miR-8516 and block the function of miR-8516 by binding to the target site of miR-8516 a negative feedback relationship existed between circRNA8220 and miR-8516. Stanniocalcin 2 (STC2) was a target gene of miR-8516. circRNA8220 could up-regulate the expression of STC2 by sponging miR-8516 in GMECs. circRNA8220/miR-8516/STC2 could promote proliferation and enhance the synthesis of β-casein and triglycerides (TG) via Ras/MEK/ERK and PI3K/AKT/mTOR signaling pathways, respectively. [ABSTRACT FROM AUTHOR]

The C-H annulation of the five-position of quinolines and acrylates to afford heterocycles is an active field of research in organic synthesis. Herein the annulation of 4-aminoquinolines with acrylates through two consecutive C-H activations catalyzed by Rh(III) is described. The reaction proceeds with high atom efficiency under mild reaction conditions, and this protocol will provide appealing strategies for the synthesis of fused quinoline heterocycles.

The elusive rhodium-catalyzed Heck-type coupling of arylboronic acids with alpha,beta-unsaturated esters is effected using rhodium chloride as catalyst, indicating the hydrolysis of Rh-C bond could be finely tuned by the steric environment around rhodium.