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Transcranial focused ultrasound (tFUS) is increasingly used in experimental neuroscience due to its neuromodulatory effectiveness in animal studies. However, achieving multitarget tFUS in small animals is typically limited by transducer size, energy transfer efficiency, and brain volume. The objective of this work was to construct an ultrasound system for multitarget neuromodulation in small animals. First, a miniaturized high-powered 2-D array transducer was developed. The phase delay of each array element was calculated based on the multifocal time-reversal method, generating multiple foci simultaneously in a 3-D field. The effects of the axial focal length, interfocus spacing (lateral distance between the two focal centers), and the number of foci on the focal properties of the pressure field were examined through numerical simulations. In-vitro ultrasonic measurements and transcranial simulations on a rat skull were conducted. The minimum interfocus spacing separating two −6-dB foci and the peak full-width at half-maximum were positively correlated with axial focal length; the relative relationship between the interfocus spacing and pressure field properties was similar for each axial focal length. The maximum acoustic pressure and spatial average intensity at focus in deionized water were 2.21 MPa and 133 W/cm2, respectively. The simulated and experimental results were compared, demonstrating agreement in both peak position and focus shape. The ultrasound system can provide a neuroscientific platform for evaluating the feasibility of multitarget ultrasound stimulation treatment protocols, thus improving the understanding of functional neuroanatomy. [ABSTRACT FROM AUTHOR]

Transcranial ultrasound therapy has become a noninvasive method for treating neurological and psychiatric disorders, and studies have further demonstrated that multitarget transcranial ultrasound therapy is a better solution. At present, multitarget transcranial ultrasound therapy in small animals can only be achieved by the multitransducer or phased array. However, multiple transducers may cause spatial interference, and the phased array system is complicated, expensive, and especially unsuitable for small animals. This study is the first to design and fabricate a miniature acoustic holography lens for multitarget transcranial ultrasound therapy in rats. The acoustic holographic lens, working at a frequency of 1.0 MHz, with a size of 10.08 mm $\times10.08$ mm and a pixel resolution of 0.72 mm, was designed, optimized, and fabricated. The dual-focus transcranial ultrasound generated based on the lens was measured; the full-width at half-maximum (FWHM) of the focal spots in the ${y}$ -direction was 2.15 and 2.27 mm and in the ${z}$ -direction was 2.3 and 2.36 mm. The focal length was 5.4 mm, and the distance between the two focuses was 5.6 mm, close to the desired values of 5.4 and 6.0 mm. Finally, the multiple-target blood–brain barrier opening in rats’ bilateral secondary visual cortex (mediolateral area, V2ML) was demonstrated using the transcranial ultrasound therapy system based on the lens. These results demonstrate the good performance of the multitarget transcranial ultrasound therapy system for small animals, including high spatial resolution, small size, and low cost. [ABSTRACT FROM AUTHOR]

Featured Application: Nondestructive testing and structural health monitoring for complex-shaped parts with a high resolution. Ultrasonic methods have been extensively developed in nondestructive testing for various materials and components. However, accurately extracting quantitative information about defects still remains challenging, especially for complex structures. Although the immersion technique is commonly used for complex-shaped parts, the large mismatch of acoustic impedance between water and metal prevents effective ultrasonic transmission and leads to a low signal-to-noise ratio(SNR). In this paper, a quantitative imaging method is proposed for complex-shaped parts based on an ice-coupled full waveform inversion (FWI) method. Numerical experiments were carried out to quantitatively inspect the various defects in a turbine blade. Firstly, the k-space pseudospectral method was applied to simulate ice-coupled ultrasonic testing for the turbine blade. The recorded full wavefields were then applied for a frequency-domain FWI based on the Limited-memory Broyden–Fletcher–Goldfarb–Shanno (L-BFGS) method. With a carefully selected iterative number and frequency, a successive-frequency FWI can well detect half wavelength defects. Extended studies on an open notch with different orientations and multiple adjacent defects proved its capability to detect different types of defects. Finally, an uncertainty analysis was conducted with inaccurate initial velocity models with a relative error of ±2%, demonstrating its robustness even with a certain inaccuracy. This study demonstrates that the proposed method has a high potential to inspect complex-shaped structures with an excellent resolution. [ABSTRACT FROM AUTHOR]

Ultrasonic guided wave (UGW) has shown great potential in the field of structural health monitoring of rail tracks due to its long-range capability and full cross section coverage. However, the practical application of UGW has been hindered by the complicated signal interpretation because of the natures of multiple modes and dispersion. Therefore, it is desirable that the effective UGW modes with high excitability and least dispersion can be identified and extracted for practical applications. In this paper, a numerical study on the guided wave propagation was carried out on a standard rail with 56E1 profile. Firstly, Floquet-Bloch theory was applied to obtain the dispersion curves of guided wave in a rail. Then, a 3D FE model was built to investigate the UGW propagation along the rail within the frequency range of 0–120 kHz. Wavenumber-frequency analysis method was applied to decompose and identify the propagating UGW modes. With a carefully designed 2D bandpass filter, a specific mode W0 was extracted in the wavenumber-frequency domain. Finally, a frequency band sweep technique was also proposed to get the optimal frequency band to achieve a pure and least-dispersive UGW mode along the rail web. The proposed method provides an effective way to extract efficient UGW modes to assess the integrity of the rail track, as well as other waveguides with complex geometry. [ABSTRACT FROM AUTHOR]

Elevator wire ropes with polymer cores hold and hoist heavy fluctuating loads in a corrosive environment. Such working condition causes metal fatigue, which together with abrasion around pulleys leads to progressive loss of the metallic cross section. This can be seen in the forms of a roughened and pitted surface of the ropes, reduction in diameter, and broken wires. Therefore, their deterioration must be monitored so that any unexpected damage or corrosion can be detected before it causes a fatal accident. Ultrasonic-guided wave-based inspection, which has proved its capability in nondestructive testing of platelike structures such as tubes and pipes, can monitor the cross section of wire ropes in their entire length from a single point. However, guided waves have drawn less attention for defect detection purposes in wire ropes. This article reports the condition monitoring of a steel wire rope from a hoisting elevator with broken wires as a result of corrosive environment and fatigue. Finite element analysis was conducted as a baseline to study guided wave propagation in wire ropes and plot dispersion curves. Guided wave propagation in wire ropes was experimentally investigated on a newly built cable stretching machine equipped with a load sensor under different amount of tensile loading. To expose the indication of broken wires, the recorded signals were analyzed by tailor-made continuous wavelet transform called tone burst wavelet. [ABSTRACT FROM AUTHOR]

Fiber Bragg gratings (FBGs) have being seen for a growing application in the field of structural health monitoring (SHM) because of their lightweight features. This letter introduces an adaptive SHM sensing system based on the ultrasonic guided wave (UGW) and FBG sensor technology. First, the capability of the proposed SHM sensing system used to detect UGW is demonstrated by the frequency detection of Rayleigh wave that is generated by the piezoelectric transducers with a function generator. Furthermore, a nanosecond pulse laser is utilized to generate Lamb wave on an aluminum plate, while the SHM sensing system is employed to detect the wave in a long distance. Finally, the SHM sensing system is used to locate the source of Rayleigh wave that is created by a pulsed laser. The experiment results show the proposed SHM sensing system is capable of localizing and monitoring the UGW by using four FBG cascaded on a loaded aluminum plate. [ABSTRACT FROM PUBLISHER]

Non-linear interaction of waves with contact interfaces has been widely applied in non-destructive evaluation fields such as bonding quality evaluation, and the detection of closed microcracks and composite delamination. This paper proposes an absolute measurement of the ultrasonic non-linearity parameter using a piezoelectric detection method for two aluminum alloy blocks of different lengths. The results of a two-dimensional finite element method model verified by models for hard and soft contact interfaces, depending on the interface property, were compared with the measured non-linearity parameter. The measured values show good agreement with the modelled results, indicating good potential for measuring the non-linearity parameter at interfaces experimentally and numerically. [ABSTRACT FROM PUBLISHER]

Ultrasonic is one of the well-known methods for surface roughness measurement, but small roughness will only lead to a subtle variation of transmission or reflection. To explore sensitive techniques for surfaces with small roughness, nonlinear ultrasonic measurement in through-transmission and pulse-echo modes was proposed and studied based on an effective unit-cell finite element (FE) model. Higher harmonic generation in solids was realized by applying the Murnaghan hyperelastic material model. This FE model was verified by comparing the absolute value of the nonlinearity parameter with the analytical solution. Then, random surfaces with different roughness values ranging from 0 μm to 200 μm were repeatedly generated and studied in the two modes. The through-transmission mode is very suitable to measure the surfaces with roughness as small as 3% of the wavelength. The pulse-echo mode is sensitive and effective to measure the surface roughness ranging from 0.78% to 5.47% of the wavelength. This study offers a potential nondestructive testing and monitoring method for the interfaces or inner surfaces of the in-service structures. [ABSTRACT FROM AUTHOR]

A decrease of piezoelectric properties in the fabrication of ultra-small Pb(Mg1/3Nb2/3)–x%PbTiO3 (PMN–x%PT) for high-frequency (>20 MHz) ultrasonic array transducers remains an urgent problem. Here, PMN–31%PT with micron-sized kerfs and high piezoelectric performance was micromachined using a 355 nm laser. We studied the kerf profile as a function of laser parameters, revealing that micron-sized kerfs with designated profiles and fewer micro-cracks can be obtained by optimizing the laser parameters. The domain morphology of micromachined PMN–31%PT was thoroughly analyzed to validate the superior piezoelectric performance maintained near the kerfs. A high piezoresponse of the samples after micromachining was also successfully demonstrated by determining the effective piezoelectric coefficient (d33*~1200 pm/V). Our results are promising for fabricating superior PMN–31%PT and other piezoelectric high-frequency (>20 MHz) ultrasonic array transducers. [ABSTRACT FROM AUTHOR]

Objective. Monoamine dysfunction has been implicated as a pathophysiological basis of several mental disorders, including anxiety and depression. Transcranial ultrasound stimulation (TUS) is a noninvasive nerve stimulation technic showing great potential in treating depression/anxiety disorders. This study aims to investigate whether TUS can ameliorate depression with anxiety in mice by regulating brain monoamine levels. Approach. Mice received repeated subcutaneous injections of corticosterone (CORT, 20 mg kg -1 ) for 3 weeks to produce depression- and anxiety-like behaviors. Ultrasound stimulated the dorsal lateral nucleus (DRN) for 30 min daily for 3 weeks without interruption of CORT injection. Behavioral phenotypes of depression and anxiety were estimated by sucrose preference test (SPT), tail suspension test (TST), and elevated plus-maze test (EPM). Liquid chromatography-mass spectrometry (LC-MS) was used to quantify brain levels of serotonin (5-HT), norepinephrine (NE), and dopamine (DA). Western blotting was performed to detect brain-derived neurotrophic factor (BDNF) levels in hippocampal. Main results. TUS of DRN significantly ameliorated the depression-like behaviors in SPT ( p = 0.0004) and TST ( p = 0.0003) as well as anxiety-like behaviors in EPM (open arm entry frequencies, p < 0.05). Moreover, TUS increased c-Fos-positive cell expression ( p = 0.0127) and induced no tissue damage. LC-MS results showed TUS of DRN resulted in a non-significant increase in the 5-HT levels and a significant decrease in the NE levels, but did not affect the levels of DA and BDNF. Significance. These results suggest TUS of DRN has safely and effectively ameliorated CORT-induced depression- and anxiety-like behaviors, possibly by restoring brain levels of 5-HT and NE. TUS may be a safe and effective technique for remedying depression and anxiety comorbidity., (© 2023 IOP Publishing Ltd.)

Objective. Exhaustion of Serotonin (5-hydroxytryptamine, 5-HT) is a typical cause of the depression disorder's development and progression, including depression-like behaviors. Transcranial ultrasound stimulation (TUS) is an emerging non-invasive neuromodulation technique treating various neurodegenerative diseases. This study aims to investigate whether TUS ameliorates depression-like behaviors by restoring 5-HT levels. Methods. The depression model mice are established by chronic restraint stress (CRS). Ultrasound waves (FF = 1.1 MHz, PRF = 1000 Hz, TBD = 0.5 ms, SD = 1 s, ISI = 1 s, and DC = 50%) were delivered into the dorsal raphe nucleus (DRN) for 30 min per day for 2 weeks. Depression-like behavior changes are evaluated with the sucrose preference and tail suspension tests. Liquid chromatography-mass spectrometry is performed to quantitatively detect the concentration of 5-HT in the DRN to explore its potential mechanism. The effectiveness and safety of TUS were assessed by c-Fos immunofluorescence and hematoxylin and eosin (HE) staining, respectively. Results. Three weeks after CRS, 22 depressive mice models were screened by sucrose preference index (SPI). After 2 weeks of ultrasound stimulation of the DRN (DRN-TUS) in depressive mice, the SPI was increased ( p = 0.1527) and the tail suspension immobility duration was significantly decreased ( p = 0.0038) compared with the non-stimulated group. In addition, TUS significantly enhances the c-Fos ( p = 0.05) positive cells' expression and the 5-HT level ( p = 0.0079) in the DRN. Importantly, HE staining shows no brain tissue damage. Conclusion. These results indicate that DRN-TUS has safely and effectively improved depression-like behaviors including anhedonia and hopelessness, potentially by reversing the depletion of 5-TH. Significance TUS may provide a new perspective on depression therapy, possibly through restoring monoamine levels., (© 2023 IOP Publishing Ltd.)

Objective. Ultrasound neuromodulation has become an emerging method for the therapy of neurodegenerative and psychiatric diseases. The phased array ultrasonic transducer enables multi-target ultrasound neuromodulation in small animals, but the relatively large size and mass and the thick cables of the array limit the free movement of small animals. Furthermore, spatial interference may occur during multi-target ultrasound brain stimulation with multiple micro transducers. Approach. In this study, we developed a miniature power ultrasound transducer and used the virtual source time inversion method and 3D printing technology to design, optimize, and manufacture the acoustic holographic lens to construct a multi-target ultrasound neuromodulation system for free-moving mice. The feasibility of the system was verified by in vitro transcranial ultrasound field measurements, in vivo dual-target blood-brain barrier (BBB) opening experiments, and in vivo dual-target ultrasound neuromodulation experiments. Main results. The developed miniature transducer had a diameter of 4.0 mm, a center frequency of 1.1 MHz, and a weight of 1.25 g. The developed miniature acoustic holographic lens had a weight of 0.019 g to generate dual-focus transcranial ultrasound. The ultrasonic field measurements' results showed that the bifocal's horizontal distance was 3.0 mm, the -6 dB focal spot width in the x -direction was 2.5 and 2.25 mm, and 2.12 and 2.24 mm in the y -direction. Finally, the in vivo experimental results showed that the system could achieve dual-target BBB opening and ultrasound neuromodulation in freely-moving mice. Significance. The ultrasonic neuromodulation system based on a miniature single-element transducer and the miniature acoustic holographic lens could achieve dual-target neuromodulation in awake small animals, which is expected to be applied to the research of non-invasive dual-target ultrasonic treatment of brain diseases in awake small animals., (© 2023 IOP Publishing Ltd.)