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This study assessed the presence of potentially toxic elements (PTEs) in China's northeastern black soil belt, an area with limited prior research. We collected 304 soil samples (0–20 cm) from Gonghe Town, Hailun City, and analyzed the PTE contamination degree using the single-factor pollution index and Nemerow pollution index. The results demonstrated that the mean concentrations of arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), mercury (Hg), nickel (Ni), lead (Pb), and zinc (Zn) were 11.16, 0.11, 65.29, 22.56, 0.03, 27.07, 26.09, and 66.01 mg/kg, respectively. Source apportionment was conducted via correlation analysis, principal component analysis, and positive matrix factorization, identifying four main sources: natural (33.2%), irrigation (29.5%), fuel (23.4%), and fertilizer (13.2%). The ecological risk index indicated a slight ecological risk, while the human health risk showed that non-carcinogenic risks were negligible and carcinogenic risks were acceptable. Our findings emphasize the need to prioritize controlling PTEs from fertilizer, particularly cadmium, and to a lesser extent, irrigation and fuel sources, focusing on As, Pband Hg. This research provides critical insights for policymakers aiming to manage PTE contamination in black soils. [ABSTRACT FROM AUTHOR]

Remote sensing provides us with an approach for the rapid identification and monitoring of spatiotemporal changes in the urban ecological environment at different scales. This study aimed to construct a remote sensing assessment index for urban ecological livability with continuous fine spatiotemporal resolution data from Landsat and MODIS to overcome the dilemma of single image-based, single-factor analysis, due to the limitations of atmospheric conditions or the revisit period of satellite platforms. The proposed Ecological Livability Index (ELI) covers five primary ecological indicators – greenness, temperature, dryness, water-wetness, and atmospheric turbidity – which are geometrically aggregated by non-equal weights based on an entropy method. Considering multisource time-series data of each indicator, the ELI can quickly and comprehensively reflect the characteristics of the Ecological Livability Quality (ELQ) and is also comparable at different time scales. Based on the proposed ELI, the urban ecological livability in the central urban area of Wuhan, China, from 2002 to 2017, in the different seasons was analyzed every 5 years. The ELQ of Wuhan was found to be generally at the medium level (ELI ≈0.6) and showed an initial trend of degradation but then improved. Moreover, the ecological livability in spring and autumn and near rivers and lakes was found to be better, whereas urban expansion has led to the outward ecological degradation of Wuhan, but urban afforestation has enhanced the environment. In general, this paper demonstrates that the ELI has an exemplary embodiment in urban ecological research, which will support urban ecological protection planning and construction. [ABSTRACT FROM AUTHOR]

Delaying the deterioration of bakery goods is necessary in the food industry. The objective of this study was to determine the effects of wheat oligopeptide (WOP) on the qualities of bread rolls. The effects of WOP on the baking properties, moisture content, and starch crystallization of rolls during the storage process were investigated in this study. The results showed that WOP effectively improved the degree of gluten cross-linking, thereby improving the specific volume and the internal structure of rolls. The FTIR and XRD results showed that the addition of WOP hindered the formation of the starch double helix structure and decreased its relative crystallinity. The DSC results revealed a decrease in the enthalpy change (ΔH) from 0.812 to 0.608 J/g after 7 days of storage with 1.0% WOP addition, further indicating that WOP reduced the availability of water for crystal lattice formation and hindered the rearrangement of starch molecules. The addition of WOP also improved the microstructure of the rolls that were observed using SEM analysis. In summary, WOP is expected to be an effective natural additive to inhibit starch staling and provide new insights into starchy food products. [ABSTRACT FROM AUTHOR]

The accumulation of potentially toxic elements (PTEs) in agricultural soils is of particular concern in China, while its status, ecological risks, and human health hazards have been little studied in the permafrost areas of Northeast China. In this study, 75 agricultural soil samples (0–20 cm) were collected from the Arctic Village, Mo'he City, in the northernmost part of China. The average concentration (mean ± standard deviation) of As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn were 12.11 ± 3.66 mg/kg, 0.11 ± 0.08 mg/kg, 52.50 ± 8.83 mg/kg, 12.08 ± 5.12 mg/kg, 0.05 ± 0.02 mg/kg, 14.90 ± 5.35 mg/kg, 22.38 ± 3.04 mg/kg, and 68.07 ± 22.71 mg/kg, respectively. Correlation analysis, cluster analysis, and principal component analysis indicated that As, Cu, Ni, and Zn likely originated from geogenic processes, Hg and Pb from long-range atmospheric transport, Cd from planting activities, and Cr from Holocene alluvium. The geo-accumulation index and enrichment factor showed that As, Cd, Hg, and Zn are enriched in soils. The Nemerow pollution index showed that 66.67%, 24%, and 1.33% of soil samples were in slight, moderate, and heavy pollution levels, respectively, with Hg being the most important element affecting the comprehensive pollution index. The potential ecological risk index showed that 48.00% and 1.33% of soil samples were in the moderate ecological risk and high potential ecological risk levels, respectively. The non-carcinogenic and carcinogenic human health risk index for adults and children were both less than 1, which was within the acceptable range. This study revealed the accumulation pattern of PTEs in agricultural soils of permafrost regions and provided a scientific basis for research on ecological security and human health. [ABSTRACT FROM AUTHOR]

The consistency of geospatial data is of great significance for the application and updating of geographic information in web maps. Due to the multiple data sources and different temporal versions, the tile web maps usually meet the inconsistency question across different layers. This study tries to develop a method to detect this kind of inconsistency utilizing a raster-based scaling approach. Compared with vector-based handling, this method can be directly available for multi-level tile images in a pixel representation form. The proposed cross-layer raster tile map rendering method (CRTMRM) consists of four primary aspects: geographic object separation, consistency rendering rules, data scaling and derivation with super-pixel segmentation, and inconsistency detection. The scale transformation strategy with the super-pixel attempts to obtain a simplified representation. Taking the scale lifespan variation and geometric consistency rules into account, the inconsistency detection of tile maps is conducted between temporal versions, multi-sources, and different scales through actual and derived data overlay analysis. The experiment focuses on features of cross-layer water or vegetation areas with Level 9 to Level 14 in Baidu Maps, Amap, and Google Maps. This method is able to serve as a basis for massive unstructured web map data inconsistency detection and support intelligent web map rendering. [ABSTRACT FROM AUTHOR]

Fe–6.5Si–2Cr–xNi (x = 4, 8, 12) high‐silicon steels with novel chemical composition are fabricated by adding Cr and Ni elements to high‐silicon steels. The experimental results show that the microstructures of FeSiCrNi high‐silicon steels are gradually refined with the increase in Ni content. In particular, as for Fe–6.5Si–2Cr–12Ni sample, the grains are refined very considerably. Fe–6.5Si–2Cr–12Ni sample comprises a large number of A2 disordered phases, a small number of ordered B2 phases, and a certain number of α‐Fe phases. The addition of Ni element inhibits the nucleation of grain boundary ferrite, and thus it contributes to the formation of acicular ferrite with high density of dislocations and high‐angle grain boundaries. In addition, the high content of Ni elements leads to slow diffusion of the atoms as well as severe thermal hysteresis in the Fe–6.5Si–2Cr–12Ni sample. This plays an important role in refining the microstructures of Fe–6.5Si–2Cr–12Ni sample. In addition, the intensity of {001}<100> cube texture and {001}<110> rotation‐cube texture increases with the increase in Ni content, which makes the Fe–6.5Si–2Cr–12Ni sample have strong λ‐fiber texture, α‐fiber texture, and γ‐fiber texture. [ABSTRACT FROM AUTHOR]

To unveil the role and regulatory mechanism of miR‐146a‐5p in sepsis. A sepsis cell model was established via lipopolysaccharide (LPS)‐induction in dendritic cells (DCs). The maturation of DCs was evaluated via flow cytometry. Gene expression was measured through reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR). The concentrations of inflammation biomarkers were revealed via enzyme‐linked immunosorbent assay (ELISA). The pathological and histological changes in lungs in the sepsis mice model were analyzed via hematoxylin and eosin (H&E) staining. In this study, the miR‐146a‐5p level was elevated in the serum of sepsis patients and LPS‐induced DCs but decreased in the serums of cured sepsis patients. Furthermore, miR‐146a‐5p deletion alleviated the activation of T cells and attenuated the imbalance of Th17/Treg. Besides, ATG7 was validated as a target of miR‐146a‐5p. ATG7 elevation enhanced lactate production and glucose uptake in LPS‐triggered DCs. Additionally, upregulation of ATG7 suppressed the protein levels of phosphorylated adenosine monophosphate‐activated protein kinase (p‐AMPK), phospho protein kinase B (p‐AKT), and phosphorylated signal transducer and activator for transcription 3 (p‐STAT3). In addition, miR‐146a‐5p downregulation alleviated T‐cell activation, inflammation, lactate production, and glucose uptake in sepsis mice. Moreover, the lung injury due to sepsis was also attenuated as a result of miR‐146a‐5p silencing. MiR‐146a‐5p aggravates sepsis through DCs activation and glycolysis via targeting ATG7. [ABSTRACT FROM AUTHOR]

Byline: Junbo Yu, Roger R. Stough Existing studies of entrepreneurship development are inadequate in measuring the scale of entrepreneurial activity, or analysing its determinants in China. The purpose of this paper is, therefore, to provide an analytical framework to better define and measure entrepreneurship in a systematic way, enabling benchmarking with other nations.

Intervertebral disc degeneration is associated with angiogenesis and is the primary cause of disc-associated disease. Several studies have indicated the importance of microRNA (miR)-21 in angiogenesis. Thus, the present study aimed to validate the role and underlying mechanisms of miR-21 in a rat model of intervertebral disc degeneration. A total of 60 specific-pathogen-free Sprague-Dawley rats were used for in vivo experiments. A rat model of intervertebral disc degeneration was established and miR-21 inhibitor (antagomiR-21) was administered. The vertebral pulp and annulus fibrosus were isolated for immunohistochemical analysis of hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) expression. Lumbar spine proteoglycan content was detected with the phloroglucinol method. Disc cell apoptosis was detected with terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling staining. It was revealed that antagomiR-21 treatment decreased the expression of HIF-1α and VEGF in the vertebral pulp and annulus fibrosus. Furthermore, antagomiR-21 treatment increased proteoglycan content and inhibited cell apoptosis in lumbar spines from model rats with intervertebral disc degeneration. In conclusion, antagomiR-21 treatment exerted a protective role in a rat model of intervertebral disc degeneration, which may provide the basis for a potential therapeutic approach in the treatment of disc-associated diseases. [ABSTRACT FROM AUTHOR]

In order to investigate the health risks of NO 3 - in rural drinking groundwater in Suihua, China and provide a basis for healthy drinking water, 40 sets of groundwater samples were collected in the Suihua area, and the average concentration of nitrate in the study area was 71.66 mg/L, statistical analysis software (SPSS19), Hydrogeochemical Analysis Software (AqQA) and groundwater pollution analysis software were used. Through water sample collection, chemical analysis and construction of human health risk model (HHRA), a qualitative and quantitative assessment of NO 3 - health risk was carried out for people of different ages and sexes, and it was concluded that there was NO 3 - pollution health risk in rural drinking groundwater in Suihua. Health risk level: infants > children > adult females > adult males. The evaluation provides a scientific basis for the prevention and control of NO 3 - pollution in groundwater and new ideas for preventing human health risks.

Objective: Rheumatoid arthritis (RA), as a chronic autoimmune disorder, seriously threatens human health. However, no study has thoroughly illustrated the etiology of RA. The present work focused on investigating the biological functions of STAT6 and the upstream miRNAs that regulate its expression., Methods: Synovial tissues from rheumatoid arthritis (RA) patients and normal participants were acquired. Cell viability, proliferation, apoptosis, concentrations of cytokines, miRNA and protein levels, and relative luciferase activities were detected., Results: WB and qRT-PCR showed that STAT6 was obviously up-regulated in synovial tissues of RA patients as well as RA fibroblast-like synoviocytes (RA FLSs). Functionally, down-regulation of STAT6 significantly inhibited the growth of RA FLSs as indicated by EdU and CCK-8 assays. In addition, inhibition of STAT6 remarkably promoted apoptosis of RA FLSs. Besides, silence of STAT6 notably suppressed inflammatory cytokine levels, such as TNF-α, IL-6 and IL-1β. Mechanistically, STAT6 was predicted to be the direct target of and negatively regulated by miR-135a-5p. Moreover, STAT6 was involved in the regulation of miR-135a-5p on cell growth, apoptosis and inflammatory response of RA FLSs., Conclusion: miR-135a-5p/STAT6 is a potential novel therapeutic target for RA treatment., Competing Interests: None., (AJTR Copyright © 2022.)

Objectives: To explore the expression levels and the potential regulatory mechanism of miR-21-5p in LPS-treated H9c2 cells., Methods: The secretions of the inflammatory cytokines induced by LPS in H9c2 cells were evaluated using ELISA. We used RT-RCR and western blot to measure the relative mRNA and protein expression levels in LPS-treated H9c2 cells. CCK-8 and EdU assays showed the viability and proliferation profiles of the H9c2 cells. TUNEL assays demonstrated the apoptotic behaviors of the H9c2 cells, and a luciferase reporter analysis was used to investigate the interactions between miR-21-5p and programmed cell death protein 4 (PDCD4)., Results: LPS induced damage to the H9c2 cells by reducing the cell viability and down-regulating miR-21-5p. On the other hand, miR-21-5p overexpression inhibited the LPS-induced inflammatory damage in the H9c2 cells. Moreover, PDCD4 was verified as a downstream target gene of miR-21-5p, and its expression was inhibited by the higher miR-21-5p content. Finally, miR-21-5p inhibited septic processes, and the PDCD4 overexpression rescued the miR-21-5p effect in the LPS-treated H9c2 cells., Conclusion: Our findings suggest that miR-21-5p inhibits the LPS-induced progression of sepsis in H9c2 cells. Additionally, PDCD4 is a downstream target gene of miR-21-5p, and both molecules serve as potential therapeutic targets for heart sepsis patients., Competing Interests: None., (AJTR Copyright © 2021.)