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Abstract The lymphatic system consists of a vessel network lined by specialized lymphatic endothelial cells (LECs) that are responsible for tissue fluid homeostasis and immune cell trafficking. The mechanisms for organ-specific LEC responses to environmental cues are not well understood. We found robust lymphangiogenesis during influenza A virus infection in the adult mouse lung. We show that the number of LECs increases twofold at 7 days post-influenza infection (dpi) and threefold at 21 dpi, and that lymphangiogenesis is preceded by lymphatic dilation. We also show that the expanded lymphatic network enhances fluid drainage to mediastinal lymph nodes. Using EdU labeling, we found that a significantly higher number of pulmonary LECs are proliferating at 7 dpi compared to LECs in homeostatic conditions. Lineage tracing during influenza indicates that new pulmonary LECs are derived from preexisting LECs rather than non-LEC progenitors. Lastly, using a conditional LEC-specific YAP/TAZ knockout model, we established that lymphangiogenesis, fluid transport and the immune response to influenza are independent of YAP/TAZ activity in LECs. These findings were unexpected, as they indicate that YAP/TAZ signaling is not crucial for these processes.

Summary: Macrophages are critical for maintenance and repair of mucosal tissues. While functionally distinct subtypes of macrophage are known to have important roles in injury response and repair in the lungs, little is known about macrophages in the proximal conducting airways. Single-cell RNA sequencing and flow cytometry demonstrated murine tracheal macrophages are largely monocyte-derived and are phenotypically distinct from lung macrophages at homeostasis. Following sterile airway injury, monocyte-derived macrophages are recruited to the trachea and activate a pro-regenerative phenotype associated with wound healing. Animals lacking the chemokine receptor CCR2 have reduced numbers of circulating monocytes and tracheal macrophages, deficient pro-regenerative macrophage activation and defective epithelial repair. Together, these studies indicate that recruitment and activation of monocyte-derived tracheal macrophages is CCR2-dependent and is required for normal airway epithelial regeneration.

The lymphatic system consists of a vessel network lined by specialized lymphatic endothelial cells (LECs) that are responsible for tissue fluid homeostasis and immune cell trafficking. The mechanisms for organ-specific LEC responses to environmental cues are not well understood. We found robust lymphangiogenesis during influenza A virus infection in the adult mouse lung. We show that the number of LECs increases twofold at 7 days post-influenza infection (dpi) and threefold at 21 dpi, and that lymphangiogenesis is preceded by lymphatic dilation. We also show that the expanded lymphatic network enhances fluid drainage to mediastinal lymph nodes. Using EdU labeling, we found that a significantly higher number of pulmonary LECs are proliferating at 7 dpi compared to LECs in homeostatic conditions. Lineage tracing during influenza indicates that new pulmonary LECs are derived from preexisting LECs rather than non-LEC progenitors. Lastly, using a conditional LEC-specific YAP/TAZ knockout model, we established that lymphangiogenesis, fluid transport and the immune response to influenza are independent of YAP/TAZ activity in LECs. These findings were unexpected, as they indicate that YAP/TAZ signaling is not crucial for these processes. [ABSTRACT FROM AUTHOR]

The presence of green roofs in urban areas improves the energy efficiency of buildings; contributes to the capture of CO2, decreasing pollution; and improves the appearance of cities, increasing their sustainability. Additionally, green roofs must include plant species with low requirements and maintenance, and thus, succulent species could be useful in Mediterranean semi-arid regions. In this work, the thermal inertia and the effect of different succulent species as thermal barriers on mitigating the increase in temperature inside experimental compartments were studied in comparison to conventional covers used in the Spanish Mediterranean for a whole year. In general, green covers were more efficient than conventional ones for controlling temperature. Thus, temperatures under green covers were up to 8 °C lower than conventional covers and 3–5 °C lower than the ambient temperature at noon on summer days. Furthermore, significant differences were found between green covers. Thus, despite having high thermal inertia, Aptenia cordifolia showed the worst temperature records, while Aeonium arboreum was the most efficient at mitigating temperature changes both on cold winter nights and hot summer days—even better than Sedum spp., a usual succulent used commercially. Our results demonstrate that succulent species are efficient materials to use as green covers to improve thermal conditions in buildings in Mediterranean cities. This also suggests that the mixture of succulent species (i.e., not only made of Sedum spp.) with different colors and textures could beautify green roofs without compromising their energy efficiency.

Lung regeneration occurs in a variety of adult mammals after surgical removal of one lung (pneumonectomy). Previous studies of murine post-pneumonectomy lung growth have identified regenerative “hotspots” in subpleural alveolar ducts; however, the cell-types participating in this process remain unclear. To identify the single cells participating in post-pneumonectomy lung growth, we used laser microdissection, enzymatic digestion and microfluidic isolation. Single-cell transcriptional analysis of the murine alveolar duct cells was performed using the C1 integrated fluidic circuit (Fluidigm) and a custom PCR panel designed for lung growth and repair genes. The multi-dimensional data set was analyzed using visualization software based on the tSNE algorithm. The analysis identified 6 cell clusters; 1 cell cluster was present only after pneumonectomy. This post-pneumonectomy cluster was significantly less transcriptionally active than 3 other clusters and may represent a transitional cell population. A provisional cluster identity for 4 of the 6 cell clusters was obtained by embedding bulk transcriptional data into the tSNE analysis. The transcriptional pattern of the 6 clusters was further analyzed for genes associated with lung repair, matrix production, and angiogenesis. The data demonstrated that multiple cell-types (clusters) transcribed genes linked to these basic functions. We conclude that the coordinated gene expression across multiple cell clusters is likely a response to a shared regenerative microenvironment within the subpleural alveolar ducts.

ObjectivesThe mesothelium, the surface layer of the heart, lung, bowel, liver, and tunica vaginalis, is a complex tissue implicated in organ-specific diseases and regenerative biology; however, the mechanism of mesothelial repair after surgical injury is unknown. Previous observations indicated seeding of denuded mesothelium by free-floating mesothelial cells may contribute to mesothelial healing. In this study, we investigated the prevalence of mesothelial cells in pleural fluid during the 7 days following pulmonary surgery.Study designFlow cytometry was employed to study pleural fluid of 45 patients after lung resection or transplantation. We used histologically validated mesothelial markers (CD71 and WT1) to estimate the prevalence of mesothelial cells.ResultsThe viability of pleural fluid cells approached 100%. Leukocytes and mesothelial cells were identified in the pleural fluid within the first week after surgery. The leukocyte concentration was relatively stable at all time points. In contrast, mesothelial cells, identified by CD71 and WT1 peaked on POD3. The broad expression of CD71 molecule in postoperative pleural fluid suggests that many of the free-floating non-leukocyte cells were activated or proliferative mesothelial cells.ConclusionWe demonstrated that pleural fluid post lung surgery is a source of mesothelial cells; most of these cells appear to be viable and, as shown by CD71 staining, activated mesothelial cells. The observed peak of mesothelial cells on POD3 is consistent with a potential reparative role of free-floating mesothelial cells after pulmonary surgery.

In many mammals, including rodents and humans, removal of one lung results in the compensatory growth of the remaining lung; however, the mechanism of compensatory lung growth is unknown. Here, we investigated the changes in morphology and phenotype of pleural cells after pneumonectomy. Between days 1 and 3 after pneumonectomy, cells expressing α-smooth muscle actin (SMA), a cytoplasmic marker of myofibroblasts, were significantly increased in the pleura compared to surgical controls (p < .01). Scanning electron microscopy of the pleural surface 3 days post-pneumonectomy demonstrated regions of the pleura with morphologic features consistent with epithelial-mesenchymal transition (EMT); namely, cells with disrupted intercellular junctions and an acquired mesenchymal (rounded and fusiform) morphotype. To detect the migration of the transitional pleural cells into the lung, a biotin tracer was used to label the pleural mesothelial cells at the time of surgery. By post-operative day 3, image cytometry of post-pneumonectomy subpleural alveoli demonstrated a 40-fold increase in biotin+ cells relative to pneumonectomy-plus-plombage controls (p < .01). Suggesting a similar origin in space and time, the distribution of cells expressing biotin, SMA, or vimentin demonstrated a strong spatial autocorrelation in the subpleural lung (p < .001). We conclude that post-pneumonectomy compensatory lung growth involves EMT with the migration of transitional mesothelial cells into subpleural alveoli.

Glasshouses in Mediterranean areas are mainly used for experimental or nursery purposes and the production of high value ornamental species. In addition, glasshouses may be covered by dust and other particles in these areas as rainfalls are relatively scarce. Consequently, the quality of photosynthetically active radiation (PAR) may be affected. In the present experiment we evaluated the effect of dirt on the PAR spectrum in glasshouses in Valencia (Spain), compared to glasshouses submitted to suitable maintenance and washing. Photosynthetic Photon Flux Density (PPFD) measurements were done in March 2017 at 13h, and visible, UV-C and near infrared regions were recorded with a portable spectrometer. PPFD maximum values differed depending on the spectral region and glass conditions, with higher values corresponding to the visible region. Thus, clean glass showed values of 609, 588 and 423 μmol/m2s for PPFDR, PPFDG and PPFDB, respectively and 436 and 34 μmol/m2s for PPFDIR and PPFDUV. In comparison to clean glass, dirty glass decreased PPFD in all regions, particularly UV and blue region.

Tipa (Tipuana tipu) is a common tree in gardens and carparks, although the shading effect of its canopy must be still characterised to assess the decrease of temperature and quality of irradiance. This work is a preliminary study aimed to assess the effect of shading of tree canopies on the diffuse irradiance pattern received at the soil level in comparison to other conditions. The shade provided by a group of Tipa trees, located at the Universitat Politècnica de València (Valencia, Spain), was evaluated in this experiment and compared to cloudy days and direct sun. Photosynthetic Photon Flux Density (PPFD) and red/near infrared ratios were recorded with a portable spectrometer. Measurements were recorded in January and February 2017, at 10h, 13h and 16h. Depending on the region of the spectrum and time of the day, PPFD values ranged from 0.05 to 0.42, 0.40 to 1.14, and 0.94 to 3.90 μmol·m-2·s-1, for Tipa shade, cloudy days and direct sun, respectively. The spectral analysis of PPFD in cloudy days revealed maximum values in the green region and minimum at near infrared region, while maximum PPFD for tipa shade was mostly found at near infrared, revealing higher importance of this spectral region compared to cloudy days.

The presence of green roofs in urban areas improves the energy efficiency of buildings; contributes to the capture of CO2, decreasing pollution; and improves the appearance of cities, increasing their sustainability. Additionally, green roofs must include plant species with low requirements and maintenance, and thus, succulent species could be useful in Mediterranean semi-arid regions. In this work, the thermal inertia and the effect of different succulent species as thermal barriers on mitigating the increase in temperature inside experimental compartments were studied in comparison to conventional covers used in the Spanish Mediterranean for a whole year. In general, green covers were more efficient than conventional ones for controlling temperature. Thus, temperatures under green covers were up to 8 °C lower than conventional covers and 3–5 °C lower than the ambient temperature at noon on summer days. Furthermore, significant differences were found between green covers. Thus, despite having high thermal inertia, Aptenia cordifolia showed the worst temperature records, while Aeonium arboreum was the most efficient at mitigating temperature changes both on cold winter nights and hot summer days—even better than Sedum spp., a usual succulent used commercially. Our results demonstrate that succulent species are efficient materials to use as green covers to improve thermal conditions in buildings in Mediterranean cities. This also suggests that the mixture of succulent species (i.e., not only made of Sedum spp.) with different colors and textures could beautify green roofs without compromising their energy efficiency. [ABSTRACT FROM AUTHOR]

Living through a pandemic and social upheaval suggests the importance of revisiting the intersections of the art and activism of Felix Gonzalez-Torres and Gregg Bordowitz. These artists’ works express their experiences of living through a pandemic and subsequent social change and draw out key human rights themes. The works’ materials, poetics, and invitations to interact offer opportunities for audiences to reflect on complex and ethically relevant social and cultural dynamics that surface during global crises, such as negotiating personal and collective interests, the politics of touch and coexistence, and cultivating resilience and strength. [ABSTRACT FROM AUTHOR]

The propagation of a free massless scalar field in a 1 + 1 -dimensional Minkowski space modeling, a wormhole is considered. The wormhole model consists on two timelike trajectories, which represent the entrance and the exit of the wormhole, connected via some transfer function that specifies how incoming modes that reach the entrance are transferred to the exit. We find that particles and energy fluxes are generically produced except for transfer functions that represent global conformal transformations. We consider several examples involving exit trajectories which are asymptotically inertial, asymptotically null, and also involving a faster-than-light motion to illustrate the peculiarities of the emitted energy fluxes and quantum correlations. [ABSTRACT FROM AUTHOR]

Pulmonary "air leaks," typically the result of pleural injury caused by lung surgery or chest trauma, result in the accumulation of air in the pleural space (pneumothorax). Air leaks are a major source of morbidity and prolonged hospitalization after pulmonary surgery. Previous work has demonstrated structural heteropolysaccharide (pectin) binding to the mouse pleural glycocalyx. The similar lectin‐binding characteristics and ultrastructural features of the human and mouse pleural glycocalyx suggested the potential application of these polymers in humans. To investigate the utility of pectin‐based polymers, we developed a simulacrum using freshly obtained human pleura. Pressure‐decay leak testing was performed with an inflation maneuver that involved a 3 s ramp to a 3 s plateau pressure; the inflation was completely abrogated after needle perforation of the pleura. Using nonbiologic materials, pressure‐decay leak testing demonstrated an exponential decay with a plateau phase in materials with a Young's modulus less than 5. In human pleural testing, the simulacrum was used to test the sealant function of four mixtures of pectin‐based polymers. A 50% high‐methoxyl pectin and 50% carboxymethylcellulose mixture demonstrated no sealant failures at transpleural pressures of 60 cmH2O. In contrast, pectin mixtures containing 50% low‐methoxyl pectin, 50% amidated low‐methoxyl pectins, or 100% carboxymethylcellulose demonstrated frequent sealant failures at transpleural pressures of 40–50 cmH2O (p < 0.001). Inhibition of sealant adhesion with enzyme treatment, dessication and 4°C cooling suggested an adhesion mechanism dependent upon polysaccharide interpenetration. We conclude that pectin‐based heteropolysaccharides are a promising air‐tight sealant of human pleural injuries. © 2018 Wiley Periodicals, Inc. J. Biomed. Mater. Res. Part B, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 799–806, 2019. [ABSTRACT FROM AUTHOR]

Lophophora williamsii is an ornamental slow growth cactus highly appreciated by cacti growers and hobbyists. Its demand is often satisfied through illegal collection of wild plants and many populations are threatened with extinction. Thus, an efficient in vitro protocol without plant growth regulators will be of great interest for conservation purposes of this cactus. Eight different germination media, combining Murashige and Skoog medium (MS, full and half-strength), sucrose (20 and 30 g L −1 ) and agar (8 and 10 g L −1 ), were used to study germination rate, number of seedlings with areoles and initial seedling development. Germination rates among culture media only differed significantly in the first 14 days after sowing (DAS), reaching 67–75% at the end of the assay (49 DAS). Remarkable interactions among media components were detected, and 20 g L −1 sucrose and 8 g L −1 agar combination gave the highest performance for both size and number of areoles. Following germination assay, a growth assay was conducted during 105 days using three growth media (GrM) at different sucrose concentration (15, 30 and 45 g L −1 ) to evaluate the increase in seedling size and number of areoles. Regardless of their initial size, 15 g L −1 sucrose provided the best results for both traits. Size increase was higher in the 4–5 mm seedling group, while increase in areoles was greater in 2-3 mm seedlings. It was possible to develop an in vitro protocol, in absence of plant growth regulators, which allows maximizing L. williamsii germination and growth during its first stages of development, which may increase the availability of plants in the market and avoid exhaustion of wild populations. Furthermore, plants grown ex situ could be reintroduced in endangered natural populations. [ABSTRACT FROM AUTHOR]

Continuous exposures to ultraviolet radiation can lead to harmful effects on human skin. Professional drivers may spend more than 8 h per day inside a vehicle. This study describes an analysis of the UVER (erythemal ultraviolet radiation) received by a driver and passenger inside a vehicle. A three-door Peugeot 206 was used for the study. VioSpor Blue Line dosimeters (with a response profile close to that of human skin) were used to measure the erythematic dose of UV radiation (able to produce erythema on human skin). Four dosimeters were placed in the driver's position and another four in the passenger's position. Daily irradiance was analyzed for a day in April using PMA radiometers. The measurements were obtained on relatively clear days from February to December 2009 between 9:30 A.M. and 3 P.M. Additionally, a prediction was made of the time required to produce an erythema on the driver's skin. UVER exposure, in some of the driver's positions, exceeds the Exposure Limits given by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). Skin protection measures should be taken into account by professional drivers to prevent the harmful effects of UVER radiation. [ABSTRACT FROM AUTHOR]

A contribution of pulmonary blood distension to alveolar opening was first proposed more than 100 years ago. To investigate the contribution of blood distension to lung mechanics, we studied control mice (normal perfusion), mice after exsanguination (absent perfusion) and mice after varying degrees of parenchymal resection (supra-normal perfusion). On inflation, mean tracheal pressures were higher in the bloodless mouse (4.0 ± 2.5 cm H2O); however, there was minimal difference between conditions on deflation (0.7 ± 0.9 cm H2O). To separate the peripheral and central mechanical effects of blood volume, multi-frequency lung impedance data was fitted to the constant-phase model. The presence or absence of blood had no effect on central airway resistance (p > .05). In contrast, measures of tissue damping (G), tissue elastance (H) and hysteresivity (η) demonstrated a significant increase in bloodless mice relative to control mice (p < .001). After varying amount of surgical resection and associated supra-normal perfusion of the remaining lung, there was an increase in G and H. Although the absolute difference in G and H increased with the amount of parenchymal resection, the proportional contribution of blood was identical in all conditions. The presence of blood in the pulmonary vasculature resulted in a constant 64 ± 5% reduction in tissue damping (G) and a 55 ± 4% reduction in tissue elastance (H). This nearly-constant contribution of blood to lung hysteresivity was only reduced by positive end-expiratory pressure (PEEP). To identify a distinct structural subset of vessels in the lung potentially contributing to these observations, vascular casting and scanning electron microscopy of the lung demonstrated morphologically distinct vascular rings at the alveolar opening. Our results suggest that intravascular blood distension, likely attributable to a subset of vessels in the alveolar entrance ring, contributes a measurable scaffolding effect to the functional recruitment of the peripheral lung. [ABSTRACT FROM PUBLISHER]