19 results on '"Yinglin Peng"'
Search Results
2. A novel evaluation model of image registration for cone‐beam computed tomography guided lung cancer radiotherapy
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Yimei Liu, Meining Chen, Jianlan Fang, Liangjie Xiao, Songran Liu, Qiwen Li, Bo Qiu, Runda Huang, Jun Zhang, and Yinglin Peng
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clinic model ,cone beam computed tomography ,dice similarity coefficient ,image‐guided radiotherapy ,lung cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The aim of the study was to establish a weighted comprehensive evaluation model (WCEM) of image registration for cone‐beam computed tomography (CBCT) guided lung cancer radiotherapy that considers the geometric accuracy of gross target volume (GTV) and organs at risk (OARs), and assess the registration accuracy of different image registration methods to provide clinical references. Methods The planning CT and CBCT images of 20 lung cancer patients were registered using diverse algorithms (bony and grayscale) and regions of interest (target, ipsilateral, and body). We compared the coverage ratio (CR) of the planning target volume (PTVCT) to GTVCBCT, as well as the dice similarity coefficient (DSC) of the GTV and OARs, considering the treatment position across various registration methods. Furthermore, we developed a mathematical model to assess registration results comprehensively. This model was evaluated and validated using CRFs across four automatic registration methods. Results The grayscale registration method, coupled with the registration of the ipsilateral structure, exhibited the highest level of automatic registration accuracy, the DSC were 0.87 ± 0.09 (GTV), 0.71 ± 0.09 (esophagus), 0.74 ± 0.09 (spinal cord), and 0.91 ± 0.05 (heart), respectively. Our proposed WCEM proved to be both practical and effective. The results clearly indicated that the grayscale registration method, when applied to the ipsilateral structure, achieved the highest CRF score. The average CRF scores, excellent rates, good rate and qualification rates were 58 ± 26, 40%, 75%, and 85%, respectively. Conclusions This study successfully developed a clinically relevant weighted evaluation model for CBCT‐guided lung cancer radiotherapy. Validation confirmed the grayscale method's optimal performance in ipsilateral structure registration.
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- 2024
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3. Flavonoids from mulberry leaves inhibit fat production and improve fatty acid distribution in adipose tissue in finishing pigs
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Yingying Liu, Yinglin Peng, Chen Chen, Huibo Ren, Ji Zhu, Yuan Deng, Qingming Cui, Xionggui Hu, Jianhua He, Huali Li, Xinghui Zhu, Yulong Yin, Jun He, and Yi Xiao
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Finishing pig ,Mulberry leaf ,Flavonoid ,Lipid metabolism ,Fatty acid ,Lard ,Animal culture ,SF1-1100 - Abstract
This study evaluated the effects of flavonoids from mulberry leaves (FML) on plasma biochemical indices, serum activities of lipid metabolism-related enzymes, fat morphology, fatty acid composition, and lipid metabolism in different adipose tissues of finishing pigs. We used 120 Chinese hybrid barrows of Berkshire and Bama mini-pigs with an average initial body weight of 45.11 ± 4.23 kg. The pigs were randomly assigned to five treatment groups and fed a control diet based on corn, soybean meal, and wheat bran or a control diet supplemented with 0.02%, 0.04%, 0.08%, or 0.16% FML. Each experimental group had six replicates (pens), with four pigs per pen. After a 7-d adaptation period, the feeding trial was conducted for 58 d. Blood and adipose tissue samples were collected from 30 pigs (one pig per pen) at the end of the test. The results showed that FML supplementation significantly decreased the feed intake to body gain ratio, the plasma concentrations of total cholesterol and free fatty acids, and the serum activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (linear or quadratic effects, P
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- 2024
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4. Integrated analysis strategy of genome-wide functional gene mining reveals DKK2 gene underlying meat quality in Shaziling synthesized pigs
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Shuaihan He, Yubei Wang, Yabiao Luo, Mingming Xue, Maisheng Wu, Hong Tan, Yinglin Peng, Kejun Wang, and Meiying Fang
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Shaziling pigs ,Whole genome sequencing ,Selective sweep ,IBD analysis ,DKK2 ,Meat quality ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Shaziling pig is a well-known indigenous breed in China who has superior meat quality traits. However, the genetic mechanism and genomic evidence underlying meat quality characteristics of Shaziling pigs are still unclear. To explore and investigate the germplasm characteristics of Shaziling pigs, we totally analyzed 67 individual’s whole genome sequencing data for the first time (20 Shaziling pigs [S], 20 Dabasha pigs [DBS], 11 Yorkshire pigs [Y], 10 Berkshire pigs [BKX], 5 Basha pigs [BS] and 1 Warthog). Results A total of 2,538,577 SNPs with high quality were detected and 9 candidate genes which was specifically selected in S and shared in S to DBS were precisely mined and screened using an integrated analysis strategy of identity-by-descent (IBD) and selective sweep. Of them, dickkopf WNT signaling pathway inhibitor 2 (DKK2), the antagonist of Wnt signaling pathway, was the most promising candidate gene which was not only identified an association of palmitic acid and palmitoleic acid quantitative trait locus in PigQTLdb, but also specifically selected in S compared to other 48 Chinese local pigs of 12 populations and 39 foreign pigs of 4 populations. Subsequently, a mutation at 12,726-bp of DKK2 intron 1 (g.114874954 A > C) was identified associated with intramuscular fat content using method of PCR–RFLP in 21 different pig populations. We observed DKK2 specifically expressed in adipose tissues. Overexpression of DKK2 decreased the content of triglyceride, fatty acid synthase and expression of relevant genes of adipogenic and Wnt signaling pathway, while interference of DKK2 got contrary effect during adipogenesis differentiation of porcine preadipocytes and 3T3-L1 cells. Conclusions Our findings provide an analysis strategy for mining functional genes of important economic traits and provide fundamental data and molecular evidence for improving pig meat quality traits and molecular breeding.
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- 2024
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5. Multi-Sequence Fusion Network via Single- Sequence CycleGANs for Improved Synthetic CT in Nasopharyngeal Carcinoma Treatment Planning
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Yimei Liu, Meining Chen, Jun Zhang, Yixuan Wang, Huikuan Gu, Chong Zhao, Zhenyu Qi, Xiaowu Deng, Shuyu Wu, and Yinglin Peng
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Synthetic CT ,multiple MR sequence ,MRI guided radiotherapy ,cycle-consistent generative adversarial network ,nasopharyngeal carcinoma ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
To investigate the effect of different MR sequences on the accuracy of Cycle-consistent Generative Adversarial Network (CycleGAN) based synthetic CT (sCT) generation in nasopharyngeal carcinoma (NPC). In this work, three sequences of MR, included T1-weighted imaging (T1WI), T2-weighted imaging (T2WI), and T1 contrast-enhanced weighted imaging (T1WIC), and planning CT (PCT) images of 151 patients with NPC were collected. The number of training, verification, and test sets were 108, 16, and 27, respectively. Three unsupervised CycleGAN-based models were trained using different sequences (single channels) as inputs, and the synergistic fusion model were used multiple channels. To assess the precision of these models, we evaluated key metrics such as mean error (ME), mean absolute error (MAE), structural similarity index (SSIM), peak signal-to-noise ratio (PSNR), and dose distribution, comparing the PCT with the sCT generated by each model. Overall, The SCTT2 image generated by T2WI model achieved superior results than those of T1WIC and T1WI for a single-sequence model. Compared to the SCTT2, the quality of the SCTFusion image generated by the fusion model was further improved, with significant differences in the MAE, SSIM, and PNSR metrics (P $112.97\pm 9.69$ vs. $90.99\pm 9.64$ , P $0.86\pm 0.02$ vs. $0.89\pm 0.02$ , P $21.66\pm 0.64$ vs. $23.28\pm 0.87$ , P $\pm ~1.05$ % and 0.73%±0.73% for the fusion model. In conclusion, our findings reveal that the CycleGAN model, particularly when employing fusion MR sequences as input, offers the highest accuracy in synthetic CT generation. Notably, T2WI images stand out as a viable option for sCT prediction in clinical settings where acquisition sequences or times are limited.
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- 2024
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6. Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs
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Chen Chen, Yitong Chang, Yuan Deng, Qingming Cui, Yingying Liu, Huali Li, Huibo Ren, Ji Zhu, Qi Liu, and Yinglin Peng
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backfat ,daweizi (dwz) pigs ,pathway ,rna sequencing ,transcript profile ,yorkshire pigs ,Zoology ,QL1-991 - Abstract
Objective Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 downregulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.
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- 2023
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7. Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies
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Hongyu Zhu, Dehuan Xie, Ying Wang, Runda Huang, Xi Chen, Yiwei Yang, Bin Wang, Yinglin Peng, Jianxin Wang, Dexin Xiao, Dai Wu, Chao-Nan Qian, and Xiaowu Deng
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Ultra-high dose rate radiotherapy ,FLASH-RT ,Tumor control ,Immune response ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background/Purpose: Investigating the antitumor effect and intratumor as well as local immune response in breast cancer-bearing mice after MV X-ray ultra-high dose rate radiotherapy (FLASH-RT) and conventional dose rate radiotherapy (CONV-RT). Materials/Methods: Six-week-old female C57BL/6 mice were inoculated subcutaneously with Py8119 and Py230 breast tumor cells in the inguinal mammary gland and administered 10 Gy abdominal 6 MV X-ray FLASH-RT (125 Gy/s) or CONV-RT (0.2 Gy/s) 15 days after tumor inoculation. Tumor and spleen tissues were obtained at different time points post-irradiation (PI) for analysis of immune cell infiltration using flow cytometry and immunohistochemical (IHC) staining. Intestine tissues were collected 3 days PI to evaluate normal tissue damage and immune cell infiltration. Results: Both FLASH-RT and CONV-RT significantly delayed tumor growth. Flow cytometry showed increased CD8+/CD3 + and CD8+/CD4 + ratios, and IHC confirmed a similar increased CD8 + T cell infiltration at 2 weeks PI in Py8119 tumor tissues in both irradiation groups. No statistical difference was observed between the irradiation groups in terms of tumor growth and increased T cell infiltration in the tumor. Unexpectedly, significantly smaller spleen weight and substantially higher CD8+/CD3 + and lower CD4+/CD3 + ratios were observed in the spleens of the FLASH-RT group than in the spleens of the non-irradiated control and CONV-RT groups 4 weeks PI. Pathological analysis revealed severe red pulp expansion in several spleens from the CONV-RT group, but not in the spleens of the FLASH-RT group. Reduced intestinal damage, macrophage and neutrophil infiltration were observed in the FLASH-RT group compared with CONV-RT group. Conclusions: FLASH-RT and CONV-RT effectively suppressed tumor growth and promoted CD8 + T cell influx into tumors. FLASH-RT can induce different splenic immune responses and reduce radiation-induced damage in the spleen and intestine, which may potentially enhance the therapeutic ratio of FLASH-RT.
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- 2023
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8. Identification of crucial modules and genes associated with backfat tissue development by WGCNA in Ningxiang pigs
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Chen Chen, Huibo Ren, Huali Li, Yuan Deng, Qingming Cui, Ji Zhu, Siyang Zhang, Jine Yu, Huiming Wang, Xiaodan Yu, Shiliu Yang, Xionggui Hu, and Yinglin Peng
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Ningxiang pig ,backfat tissue ,different developmental stage ,WGCNA ,hub gene ,lipid metabolism ,Genetics ,QH426-470 - Abstract
Fat deposition is an economically important trait in pigs. Ningxiang pig, one of the four famous indigenous breeds in China, is characterized by high fat content. The underlying gene expression pattern in different developmental periods of backfat tissue remains unclear, and the purpose of this investigation is to explore the potential molecular regulators of backfat tissue development in Ningxiang pigs. Backfat tissue (three samples for each stage) was initially collected from different developmental stages (60, 120, 180, 240, 300, and 360 days after birth), and histological analysis and RNA sequencing (RNA-seq) were then conducted. Fragments per kilobase of transcript per million (FPKM) method was used to qualify gene expressions, and differentially expressed genes (DEGs) were identified. Furthermore, strongly co-expressed genes in modules, which were named by color, were clustered by Weighted gene co-expression network analysis (WGCNA) based on dynamic tree cutting algorithm. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment were subsequently implemented, and hub genes were described in each module. Finally, QPCR analysis was employed to validate RNA-seq data. The results showed that adipocyte area increased and adipocyte number decreased with development of backfat tissue. A total of 1,024 DEGs were identified in five comparison groups (120 days vs. 60 days, 180 days vs. 120 days, 240 days vs. 180 days, 300 days vs. 240 days, and 360 days vs. 300 days). The turquoise, red, pink, paleturquoise, darkorange, and darkgreen module had the highest correlation coefficient with 60, 120, 180, 240, 300, and 360 days developmental stage, while the tan, black and turquoise module had strong relationship with backfat thickness, adipocyte area, and adipocyte number, respectively. Thirteen hub genes (ACSL1, ACOX1, FN1, DCN, CHST13, COL1A1, COL1A2, COL6A3, COL5A1, COL14A1, OAZ3, DNM1, and SELP) were recognized. ACSL1 and ACOX1 might perform function in the early developmental stage of backfat tissue (60 days), and FN1, DCN, COL1A1, COL1A2, COL5A1, COL6A3, and COL14A1 have unignorable position in backfat tissue around 120 days developmental stage. Besides, hub genes SELP and DNM1 in modules significantly associated with backfat thickness and adipocyte area might be involved in the process of backfat tissue development. These findings contribute to understand the integrated mechanism underlying backfat tissue development and promote the progress of genetic improvement in Ningxiang pigs.
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- 2023
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9. Genome-Wide Association Study and Identification of Candidate Genes for Intramuscular Fat Fatty Acid Composition in Ningxiang Pigs
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Qinghua Zeng, Hu Gao, Shishu Yin, Yinglin Peng, Fang Yang, Yawei Fu, Xiaoxiao Deng, Yue Chen, Xiaohong Hou, Qian Wang, Zhao Jin, Gang Song, Jun He, Yulong Yin, and Kang Xu
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longissimus dorsi ,saturated fatty acids ,monounsaturated fatty acids ,polyunsaturated fatty acids ,GWAS ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Ningxiang pigs exhibit a diverse array of fatty acids, making them an intriguing model for exploring the genetic underpinnings of fatty acid metabolism. We conducted a genome-wide association study using a dataset comprising 50,697 single-nucleotide polymorphisms (SNPs) and samples from over 600 Ningxiang pigs. Our investigation yielded novel candidate genes linked to five saturated fatty acids (SFAs), four monounsaturated fatty acids (MUFAs), and five polyunsaturated fatty acids (PUFAs). Significant associations with SFAs, MUFAs, and PUFAs were found for 37, 21, and 16 SNPs, respectively. Notably, some SNPs have significant PVE, such as ALGA0047587, which can explain 89.85% variation in Arachidic acid (C20:0); H3GA0046208 and DRGA0016063 can explain a total of 76.76% variation in Elaidic Acid (C18:1n-9(t)), and the significant SNP ALGA0031262 of Arachidonic acid (C20:4n-6) can explain 31.76% of the variation. Several significant SNPs were positioned proximally to previously reported genes. In total, we identified 11 candidate genes (hnRNPU, CEPT1, ATP1B1, DPT, DKK1, PRKG1, EXT2, MEF2C, IL17RA, ITGA1 and ALOX5), six candidate genes (ALOX5AP, MEDAG, ISL1, RXRB, CRY1, and CDKAL1), and five candidate genes (NDUFA4L2, SLC16A7, OTUB1, EIF4E and ROBO2) associated with SFAs, MUFAs, and PUFAs, respectively. These findings hold great promise for advancing breeding strategies aimed at optimizing meat quality and enhancing lipid metabolism within the intramuscular fat (IMF) of Ningxiang pigs.
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- 2023
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10. Mulberry leaf powder regulates antioxidative capacity and lipid metabolism in finishing pigs
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Yingying Liu, Yinghui Li, Yi Xiao, Yinglin Peng, Jianhua He, Chen Chen, Dingfu Xiao, Yulong Yin, and Fengna Li
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Mulberry ,Xiangcun black pig ,Antioxidative capacity ,Fatty acid ,Lipid metabolism ,Animal culture ,SF1-1100 - Abstract
This study evaluated the potential of mulberry leaf powder as an unconventional feed material for finishing pigs by assessing the growth performance, antioxidative properties, fatty acid profile, and lipid metabolism in 180 Xiangcun black pigs. Pigs with an initial body weight (BW) of 71.64 ± 1.46 kg were randomly assigned to 5 treatment groups, including the control diet and 4 experimental diets. The corn, soybean meal, and wheat bran in the control diet were partly replaced by 3%, 6%, 9%, or 12% mulberry leaf powder in experimental diets. There were 6 replicates (pens) of 6 pigs per replicate in each treatment. Blood and muscle samples were collected after the 50-day feed experiment. Compared with the control group, the 3%, 6%, and 9% mulberry diets had no adverse effect (P > 0.05) on the growth performance of pigs. The serum glutathione peroxidase activity and glutathione concentration increased linearly (P
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- 2021
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11. Multivariate NTCP Model of Hypothyroidism After Intensity-Modulated Radiotherapy for Nasopharyngeal Carcinoma
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Guanzhu Shen, Yinglin Peng, Jian Li, Haijun Wu, Guangshun Zhang, Chong Zhao, and Xiaowu Deng
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nasopharyngeal carcinoma ,intensity-modulated radiotherapy ,hypothyroidism ,EQD2 ,NTCP mode ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ObjectiveTo evaluate the incidence of hypothyroidism in patients with nasopharyngeal carcinoma after intensity-modulated radiotherapy (IMRT), analyze its correlation with multiple influencing factors such as thyroid exposure dose, thyroid volume, and gender, and construct a multivariate-based normal tissue complication probability (NTCP) model for the occurrence of hypothyroidism after IMRT.Materials and MethodsThe thyroid hormone levels of patients at different points in time before and after radiotherapy were tested, and statistics on the incidence of hypothyroidism after treatment were obtained. The dose-volume data of patients’ thyroids were converted into EQD2 equivalent dose values. The correlation between hypothyroidism after radiotherapy and thyroid exposure dose, thyroid volume, gender, and other factors was analyzed, and an NTCP model was constructed.ResultsA total of 69 patients with nasopharyngeal carcinoma were enrolled in this study. Twelve months after radiotherapy, a total of 24 patients (34.8%) developed hypothyroidism. Univariate analysis and multivariate analysis revealed that the average thyroid dose and thyroid volume are the most important factors affecting hypothyroidism after radiotherapy. The NTCP model constructed based on the average dose and thyroid volume has a good degree of fit.ConclusionThe volume and average dose of the thyroid gland are the key factors affecting the occurrence of hypothyroidism in patients with nasopharyngeal carcinoma after radiotherapy. The NTCP model constructed based on multivariate construction suggests that reducing the average dose of the thyroid to the greatest extent is an effective way to protect thyroid functions.
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- 2021
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12. Analysis of measurement electrode location in bladder urine monitoring using electrical impedance
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Yaning Li, Yinglin Peng, Xin Yang, Shipei Lu, Jinwu Gao, Chengguang Lin, and Rihui Li
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Computational simulation ,Electrical impedance ,Urine monitor ,Medical technology ,R855-855.5 - Abstract
Abstract Purpose The aim of this study was to document more appropriate electrode location of a four-electrode-based electrical impedance technology in the monitoring of bladder filling, and to characterize the relationship between bladder filling duration and the measured electrical impedances. Methods A simulation study, based on a 2-dimension computational model, was conducted to determine the preferable locations of excitation and measurement electrodes in a conventional four-electrode setup. A human observation study was subsequently performed on eight healthy volunteers during natural bladder urine accumulation to validate the result of the simulation study. The correlation between the bladder filling time and the measured electrical impedance values was evaluated. Results The preferable location of measurement electrodes was successively validated by the model simulation study and human observation study. Result obtained via the selected electrodes location revealed a significant negative correlation (R = 0.916 ± 0.059, P
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- 2019
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13. Comparison of Different Combinations of Irradiation Mode and Jaw Width in Helical Tomotherapy for Nasopharyngeal Carcinoma
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Jun Zhang, Yinglin Peng, Shouliang Ding, Jinhan Zhu, Yimei Liu, Meining Chen, Wenzhao Sun, Linghong Zhou, and Xiaowu Deng
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helical tomotherapy ,nasopharyngeal carcinoma ,dynamic jaw ,irradiation mode ,jaw width ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Purpose: To aid in the selection of a suitable combination of irradiation mode and jaw width in helical tomotherapy (HT) for the treatment of nasopharyngeal carcinoma (NPC).Materials and Methods: Twenty patients with NPC who underwent radiotherapy were retrospectively selected. Four plans using a jaw width of 2.5 or 5-cm in dynamic jaw (DJ) or fix jaw (FJ) modes for irradiation were designed (2.5DJ, 2.5FJ, 5.0DJ, and 5.0FJ). The dose parameters of planning target volume (PTV) and organs at risk (OARs) of the plans were compared and analyzed, as well as the beam on time (BOT) and monitor unit (MU). The plans in each group were ranked by scoring the doses received by the OARs and the superity was assessed in combination with the planned BOT and MU.Results: The prescribed dose coverage of PTV met the clinical requirements for all plans in the four groups. The groups using a 2.5-cm jaw width or a DJ mode provided better protection to most OARs, particularly for those at the longitudinal edges of the PTV (P < 0.05). The 2.5DJ group had the best ranking for OAR-dose, followed by the 2.5FJ and 5.0DJ groups with a same score. The BOT and MU of the groups using a 5.0-cm jaw width reduced nearly 45% comparing to those of the 2.5-cm jaw groups.Conclusion: 2.5DJ has the best dose distribution, while 5.0DJ has satisfactory dose distribution and less BOT and MU that related to the leakage dose. Both 2.5DJ or 5DJ were recommended for HT treatment plan for NPC based on the center workload.
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- 2020
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14. A Study on Changes of Carcass, Bones and Muscles of Ningxiang Pigs.
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Wei Zhang, Yinglin Peng, Qingming Cui, Yitong Chang, Yuan Deng, Chen Chen, and Yingying Liu
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MEAT quality , *LARGE intestine , *BIVARIATE analysis , *MUSCLE growth , *SMALL intestine , *ERECTOR spinae muscles - Abstract
Background: Ningxiang pigs are known for tasty meat and high intramuscular fat content. Currently, the slow growth rate and low lean meat rate of Ningxiang pigs have greatly restricted the development of its market. Therefore, the objective of this study is to explore the changes in carcass traits, meat quality, visceral organ weight indices, bones and muscles development with slaughter ages of Ningxiang pigs. Methods: The experiment used 0, 60, 120, 180, 240, 300 and 360 days old Ningxiang pigs as the research objects, and 6 castrated male pigs in each group were randomly selected. The carcass traits, meat quality, visceral organs weights and skeletal muscle weights were measured. The correlation between carcass traits and meat quality were analyzed by bivariate analysis, and the regression equations between slaughter ages and these indicators were established. Result: The results showed that pre-slaughter weight and weights of carcass, head, feet, skeleton and muscle, loin eye area, cooking yield, marbling and the large intestine weight index at 0 d, backfat thickness at 60 d, fat percentages at 60 and 120 d and the lean meat percentage at 240 d, redness at 120 d of Ningxiang pigs were lowest, the heart, lung, kidney and small intestine weight percentages were highest at 0 d (P<0.05). Carcass traits and meat quality had different significant correlation at different slaughter ages. So the indices of carcass, meat quality, bones and muscles of Ningxiang pigs changed with slaughter ages. In general, the overall performance of 300 d Ningxiang pigs is better. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Mulberry leaf powder regulates antioxidative capacity and lipid metabolism in finishing pigs
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Fengna Li, Yinghui Li, Yingying Liu, Jianhua He, Yulong Yin, Yi Xiao, Dingfu Xiao, Yinglin Peng, and Chen Chen
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Soybean meal ,Body weight ,SF1-1100 ,03 medical and health sciences ,chemistry.chemical_compound ,Animal science ,Food Animals ,Antioxidative capacity ,Original Research Article ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Bran ,0402 animal and dairy science ,food and beverages ,Fatty acid ,Lipid metabolism ,04 agricultural and veterinary sciences ,Glutathione ,Glutathione peroxidase activity ,040201 dairy & animal science ,Animal culture ,chemistry ,Animal Science and Zoology ,Mulberry leaf ,Mulberry ,Xiangcun black pig - Abstract
This study evaluated the potential of mulberry leaf powder as an unconventional feed material for finishing pigs by assessing the growth performance, antioxidative properties, fatty acid profile, and lipid metabolism in 180 Xiangcun black pigs. Pigs with an initial body weight (BW) of 71.64 ± 1.46 kg were randomly assigned to 5 treatment groups, including the control diet and 4 experimental diets. The corn, soybean meal, and wheat bran in the control diet were partly replaced by 3%, 6%, 9%, or 12% mulberry leaf powder in experimental diets. There were 6 replicates (pens) of 6 pigs per replicate in each treatment. Blood and muscle samples were collected after the 50-day feed experiment. Compared with the control group, the 3%, 6%, and 9% mulberry diets had no adverse effect (P > 0.05) on the growth performance of pigs. The serum glutathione peroxidase activity and glutathione concentration increased linearly (P
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- 2020
16. Computed Tomography-Based Evaluation of Volume and Position Changes of the Target Region and Organs at Risk During Radiotherapy for Esophageal Cancer: A Pilot Study
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Xiaowu Deng, Qiwen Li, Jun Zhang, Hui Liu, Ya-Ru Ma, Bo Qiu, Yinglin Peng, Yimei Liu, Guanzhu Shen, Li-Rong Fu, and Meining Chen
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Cancer Research ,medicine.medical_treatment ,Planning target volume ,Computed tomography ,Volume change ,03 medical and health sciences ,0302 clinical medicine ,medicine ,esophageal cancer ,geometric center deviation ,RC254-282 ,Original Research ,medicine.diagnostic_test ,business.industry ,Isocenter ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Esophageal cancer ,medicine.disease ,Gross tumor volume ,Radiation therapy ,volume changed ratio ,Oncology ,adaptive radiotherapy ,030220 oncology & carcinogenesis ,target retraction ,business ,Nuclear medicine ,030217 neurology & neurosurgery ,Volume (compression) - Abstract
ObjectiveTo analyze changes in volume and position of target regions and organs at risk (OARs) during radiotherapy for esophageal cancer patients.MethodsOverall, 16 esophageal cancer patients who underwent radiotherapy, including 10 cases of intensity-modulated radiation therapy (IMRT) and six of three-dimensional conformal radiotherapy (3D-CRT), were enrolled. The prescription doses for the planning target volumes (PTVs) were as follows: PTV1, 64 Gy/32 fractions; and PTV2, 46 Gy/23 fractions. Repeat computed tomography (CT) was performed for patients after the 5th, 10th, 15th, 20th, and 25th fractions. Delineation of the gross tumor volume (GTV) and OAR volume was determined using five repeat CTs performed by the same physician. The target and OAR volumes and centroid positions were recorded and used to analyze volume change ratio (VCR), center displacement (ΔD), and changes in the distance from the OAR centroid positions to the planned radiotherapy isocenter (distance to isocenter, DTI) during treatment.ResultsNo patient showed significant changes in target volume (TV) after the first week of radiotherapy (five fractions). However, TV gradually decreased over the following weeks, with the rate slowing after the fourth week (40 Gy). The comparison of TV from baseline to 40 Gy (20 fractions) showed that average GTVs decreased from 130.7 ± 63.1 cc to 92.1 ± 47.2 cc, with a VCR of −29.21 ± 13.96% (ppConclusionDuring radiotherapy for esophageal cancer, Targets and OARs change significantly in volume and position during the 2nd–4th weeks. Image-guidance and evaluation of dosimetric changes are recommended for these fractions of treatment to appropriate adjust treatment plans.
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- 2021
17. Long-term Survivals, Toxicities and the Role of Chemotherapy in Early-Stage Nasopharyngeal Carcinoma Patients Treated with Intensity-Modulated Radiation Therapy: A Retrospective Study with 15-Year Follow-up.
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Lin Wang, Jingjing Miao, Huageng Huang, Boyu Chen, Xiao Xiao, Manyi Zhu, Yingshan Liang, Weiwei Xiao, Shaomin Huang, Yinglin Peng, Xiaowu Deng, Xing Lv, Weixiong Xia, Yanqun Xiang, Xiang Guo, Fei Han, and Chong Zhao
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NASOPHARYNX cancer ,RADIOTHERAPY ,LACTATE dehydrogenase ,INTENSITY modulated radiotherapy ,CRANIAL nerves - Abstract
Purpose This study was aimed to investigate long-term survivals and toxicities of early-stage nasopharyngeal carcinoma (NPC) in endemic area, evaluating the role of chemotherapy in stage II patients. Materials and Methods Totally 187 patients with newly diagnosed NPC and restaged American Joint Committee on Cancer/International Union Against Cancer 8th T1-2N0-1M0 were retrospectively recruited. All received intensity-modulated radiotherapy (IMRT)±chemotherapy (CT) from 2001 to 2010. Results With 15.7-year median follow-up, 10-year locoregional recurrence-free survival, distant metastasis-free survival (DMFS), disease-specific survival (DSS), and overall survival (OS) were 93.3%, 93.5%, 92.9% and 88.2%, respectively. Multivariable analyses showed cervical lymph nodes positive and pre-treatment prognostic nutritional index = 52.0 could independently predict DMFS (p=0.036 and p=0.011), DSS (p=0.014 and p=0.026), and OS (p=0.002 and p < 0.001); Charlson comorbidity index < 3 points could predict DSS (p=0.011); age > 45 years (p=0.002) and pre-treatment lactate dehydrogenase = 240 U/L (p < 0.001) predicted OS. No grade 4 late toxicity happened; grade 3 late toxicities included subcutaneous fibrosis (4.3%), deafness or otitis (4.8%), skin dystrophy (2.1%), and xerostomia (1.1%). No differences on survivals were shown between IMRT+CT vs. IMRT alone in stage II patients, even in T2N1M0 (p > 0.05). Unsurprising, patients in IMRT+CT had more acute gastrointestinal reaction, myelosuppression, mucositis, late ear toxicity, and cranial nerve injury (all p < 0.05) than IMRT alone group. Conclusion Superior tumor control and satisfying long-term outcomes could be achieved with IMRT in early-stage NPC with mild late toxicities. As CT would bring more toxicities, it should be carefully performed to stage II patients. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
18. Analysis of measurement electrode location in bladder urine monitoring using electrical impedance
- Author
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Shipei Lu, Yinglin Peng, Li Yaning, Xin Yang, Chengguang Lin, Rihui Li, and Jinwu Gao
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Adult ,Male ,lcsh:Medical technology ,Materials science ,Urine volume ,0206 medical engineering ,Urinary Bladder ,Biomedical Engineering ,02 engineering and technology ,Urine ,Biomaterials ,Young Adult ,Healthy volunteers ,Electric Impedance ,Humans ,Radiology, Nuclear Medicine and imaging ,Urine monitor ,Computer Simulation ,Electrical impedance ,Electrodes ,Monitoring, Physiologic ,Radiological and Ultrasound Technology ,Research ,General Medicine ,020601 biomedical engineering ,Bladder filling ,Healthy Volunteers ,lcsh:R855-855.5 ,Electrode location ,Electrode ,Electrical impedance technique ,Computational simulation ,Female ,Tomography, X-Ray Computed ,Biomedical engineering - Abstract
Purpose The aim of this study was to document more appropriate electrode location of a four-electrode-based electrical impedance technology in the monitoring of bladder filling, and to characterize the relationship between bladder filling duration and the measured electrical impedances. Methods A simulation study, based on a 2-dimension computational model, was conducted to determine the preferable locations of excitation and measurement electrodes in a conventional four-electrode setup. A human observation study was subsequently performed on eight healthy volunteers during natural bladder urine accumulation to validate the result of the simulation study. The correlation between the bladder filling time and the measured electrical impedance values was evaluated. Results The preferable location of measurement electrodes was successively validated by the model simulation study and human observation study. Result obtained via the selected electrodes location revealed a significant negative correlation (R = 0.916 ± 0.059, P
- Published
- 2019
19. miR-135a-5p inhibits 3T3-L1 adipogenesis through activation of canonical Wnt/β-catenin signaling.
- Author
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Chen Chen, Yongdong Peng, Yinglin Peng, Jian Peng, and Siwen Jiang
- Subjects
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MICRORNA , *GENE expression , *ADIPOGENESIS , *NON-coding RNA , *CELL differentiation , *ADENOMATOUS polyposis coli , *ADIPOSE tissues - Abstract
MicroRNAs are endogenous, conserved, and non-coding small RNAs that function as post-transcriptional regulators of fat development and adipogenesis. Adipogenic marker genes, such as CCAAT/enhancer binding protein α (Cebpa), peroxisome proliferatoractivated receptor γ (Pparg), adipocyte fatty acid binding protein (Ap2), and fatty acid synthase (Fas), are regarded as the essential transcriptional regulators of preadipocyte differentiation and lipid storage in mature adipocytes. Canonical Wnt/β-catenin signaling is recognized as a negative molecular switch during adipogenesis. In the present work we found that miR-135a-5p is markedly downregulated during the process of 3T3-L1 preadipocyte differentiation. Overexpression of miR-135a-5p impairs the expressions of adipogenic marker genes as well as lipid droplet accumulation and triglyceride content, indicating the importance of miR-135a-5p for adipogenic differentiation and adipogenesis. Further studies show that miR-135a-5p directly targets adenomatous polyposis coli (Apc), contributes to the translocation of β-catenin from cytoplasm to nucleus, and then activates the expressions of cyclin D1 (Ccnd1) and Cmyc, indicating the induction of canonical Wnt/β-catenin signaling. In addition, inhibition of APC with siRNA exhibits the same effects as overexpression of miR-135a-5p. Our findings demonstrate that miR-135a-5p suppresses 3T3-L1 preadipocyte differentiation and adipogenesis through the activation of canonical Wnt/β-catenin signaling by directly targeting Apc. Taken together, these results offer profound insights into the adipogenesis mechanism and the development of adipose tissue. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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