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The concentration of viruses in sewage sludge is significantly higher (10–1000-fold) than that found in natural environments, posing a potential risk for human and animal health. However, the composition of these viruses and their role in the transfer of pathogenic factors, as well as their role in the carbon, nitrogen, and phosphorus cycles remain poorly understood. In this study, we employed a shotgun metagenomic approach to investigate the pathogenic bacteria and viral composition and function in two wastewater treatment plants located on a campus. Our analysis revealed the presence of 1334 amplicon sequence variants (ASVs) across six sludge samples, with 242 ASVs (41.22% of total reads) identified as pathogenic bacteria. Arcobacter was found to be the most dominant pathogen accounting for 6.79% of total reads. The virome analysis identified 613 viral genera with Aorunvirus being the most abundant genus at 41.85%. Approximately 0.66% of these viruses were associated with human and animal diseases. More than 60% of the virome consisted of lytic phages. Host prediction analysis revealed that the phages primarily infected Lactobacillus (37.11%), Streptococcus (21.11%), and Staphylococcus (7.11%). Furthermore, our investigation revealed an abundance of auxiliary metabolic genes (AMGs) involved in carbon, nitrogen, and phosphorus cycling within the virome. We also detected a total of 113 antibiotic resistance genes (ARGs), covering major classes of antibiotics across all samples analyzed. Additionally, our findings indicated the presence of virulence factors including the clpP gene accounting for approximately 4.78%, along with toxin genes such as the RecT gene representing approximately 73.48% of all detected virulence factors and toxin genes among all samples analyzed. This study expands our understanding regarding both pathogenic bacteria and viruses present within sewage sludge while providing valuable insights into their ecological functions. [ABSTRACT FROM AUTHOR]

In order to ensure the lateral stability of adaptive cruise control (ACC) vehicles during curve following, this paper proposes a hierarchical multi‐objective coordinated control ACC system structure for in‐wheel motor drive electric vehicles. Different control strategies are designed for the cruise mode and car‐following mode of the ACC system. A proportion integral differential (PID) controller is used to meet the need for speed control in cruise mode. For the car‐following mode, a multi‐objective ACC system distance control strategy is proposed considering the performance of car following, passenger comfort, driving safety and driving economy of ACC vehicles. The weight coefficients in the objective function are updated in real time by using the fuzzy model predictive control (FMPC) strategy to meet the needs of different road conditions and improve the driving economy. Then, the desired front wheel steering angle is obtained by preview control theory. According to the theory of sliding mode control (SMC), a lateral stability controller is designed to track the desired yaw rate and sideslip angle. Simulation results show that the electric vehicle driven by in‐wheel motors based on FMPC and SMC has better car‐following performance and lateral stability performance under variable curvature curved road conditions. [ABSTRACT FROM AUTHOR]

To ensure lateral stability during the cruising of a differential steering vehicle (DSV), this paper presents a curving adaptive cruise control (ACC) system coordinated with a differential steering control (DSC) system, which considers both longitudinal cruising capability and lateral stability on curved roads. Firstly, a DSV dynamics model is developed and a control strategy architecture for a curving ACC system is designed. Then, the car-following control strategy for the curving ACC system is designed based on the fuzzy model predictive control (FMPC) algorithm. The strategy aims to improve the economy and balances car following, safety, comfort and economy. Moreover, fuzzy logic rules are designed to update the weight coefficients of the performance indicators in real time. Finally, the lateral stability controller is designed based on the preview algorithm and the sliding mode control (SMC) algorithm. The simulation results show that the lateral stability of the DSV during the curving cruise is realized via the control of the differential drive torque of the two front wheels. The proposed FMPC controller and SMC controller based on the preview control algorithm satisfy the performance in terms of vehicle following and lateral stable driving in the process of cruising. [ABSTRACT FROM AUTHOR]

The purpose of this paper is to study the control of differential steering for four-in-wheel-motor electric vehicles. The so-called differential steering means that the front wheel steering is realized through the differential driving torque between the left and right front wheels. With the consideration of tire friction circle, a hierarchical control method is proposed to realize the differential steering and the constant longitudinal speed simultaneously. Firstly, the dynamic models of the front wheel differential steering vehicle, the front wheel differential steering system and the reference vehicle are established. Secondly, the hierarchical controller is designed. The upper controller is to obtain the resultant forces and resultant torque required by the front wheel differential steering vehicle tracking the reference model through the sliding mode controller. In the middle controller, the minimum tire load ratio is selected as the objective function. Combined with the constraints, the resultant forces and resultant torque are decomposed into the longitudinal and lateral forces of four wheels by the quadratic programming method. The lower controller provides the required longitudinal forces and tire sideslip angles for the front wheel differential steering vehicle model through the tire inverse model and the longitudinal force superposition scheme. Simulation results show that the hierarchical controller can guarantee the vehicle to track the reference model well on both of the high and low adhesion coefficient road with all of the tire load ratios smaller than 1. It can be drawn that the control strategy proposed in this paper is effective. [ABSTRACT FROM AUTHOR]

Background: There is increasing evidence on the prognostic significance of D-dimer and fibrinolysis in stroke. However, the systematic analysis of their relationship with adverse outcomes after stroke is lacking. Herein, we comprehensively assessed the correlation of D-dimer and fibrinolysis with stroke outcomes through meta-analysis. Methods: Studies for systematic literature review were retrieved from PubMed, EMBASE, and Cochrane Library databases. The association of D-dimer and fibrinolysis with outcomes of stroke patients was expressed as an odds ratio (OR) with 95% confidence intervals (95% CI). Results: Totally, 52 studies comprising 21,473 stroke patients were included. The results showed that the high D-dimer level was significantly associated with peripheral venous thrombosis after stroke (OR 1.03, 95% CI 1.01–1.05), poor outcome (MRS >2) after stroke (OR 1.731, 95% CI 1.464–2.048), death after stroke (OR 2.367, 95% CI 1.737–3.224), stroke recurrence (OR 1.229, 95% CI 1.113–1.358), and early neurologic deterioration (NIHSS >4) (OR 1.791, 95% CI 1.117–2.870). Moreover, high fibrinogen level was significantly associated with poor outcome (MRS >2) after stroke (OR 1.650, 95% CI 1.314–2.071), death after stroke (OR 1.310, 95% CI 1.128–1.520), stroke recurrence (OR 1.228, 95% CI 1.166–1.422), early neurologic deterioration (NIHSS >4) (OR 2.381, 95% CI 1.156–4.904), and coronary events after stroke (OR 1.427, 95% CI 1.232–1.653). Conclusion: Fibrinogen and D-dimer may be associated with adverse outcomes in patients with stroke, suggesting that they may serve as possible biomarkers for post-stroke adverse outcomes. [ABSTRACT FROM AUTHOR]

The differential steering can be used not only as the backup system of steer-by-wire, but also as the only steering system. Because the differential steering is realized through the differential moment between the coaxial left and right driving wheels, the sharp reduction of the load on the inner driving wheel will directly lead to the failure of the differential steering when the four-wheel independent drive electric vehicle approaches the rollover. Therefore, this paper not only realizes the trajectory tracking of autonomous ground vehicle through the differential steering, but also puts forward the body attitude control to improve the handling stability. Firstly, the dynamic and kinematic models of differential steering autonomous ground vehicle (DSAGV) and its roll model are established, and the linear three-degree of freedom vehicle model is selected as the reference model to generate the ideal body roll angle. Secondly, a model predictive controller (MPC) is designed to control the DSAGV to track the given reference trajectory, and obtain the required differential moment and the resulting front-wheel steering angle. Then, a sliding mode controller (SMC) is adopted to control the DSAGV to track the ideal body roll angle, and obtain the required roll moment. The simulation results show that the proposed MPC and SMC can not only make the DSAGV realize the trajectory tracking, but also achieve the body attitude control. [ABSTRACT FROM AUTHOR]

A combined system consisting of a high-temperature proton exchange membrane fuel cell (HT-PEMFC) and an organic Rankine cycle (ORC) is provided for automotive applications in this paper. The combined system uses HT-PEMFC stack cathode exhaust gas to preheat the inlet gas and the ORC to recover the waste heat from the stack. The model of the combined system was developed and the feasibility of the model was verified. In addition, the evaluation index of the proposed system was derived through an energy and exergy analysis. The numerical simulation results show that the HT-PEMFC stack, cathode heat exchanger, and evaporator contributed the most to the total exergy loss of the system. These components should be optimized as a focus of future research to improve system performance. The lower current density increased the ecological function and the system efficiency, but reduced the system's net out-power. A higher inlet temperature and higher hydrogen pressures of the stack and the lower oxygen pressure helped improve the system performance. Compared to the HT-PEFC system without an ORC subsystem, the output power of the combined system was increased by 12.95%. [ABSTRACT FROM AUTHOR]

Prostate cancer (PCa) is a common malignant cancer of the urinary system. Drug therapy, chemotherapy, and radical prostatectomy are the primary treatment methods, but drug resistance and postoperative recurrence often occur. Therefore, seeking novel anti-tumor compounds with high efficiency and low toxicity from natural products can produce a new tumor treatment method. Matijin-Su [N-(N-benzoyl-L-phenylalanyl)-O-acetyl-L-phenylalanol, MTS] is a phenylalanine dipeptide monomer compound that is isolated from the Chinese ethnic medicine Matijin (Dichondra repens Forst.). Its derivatives exhibit various pharmacological activities, especially anti-tumor. Among them, the novel MTS derivative HXL131 has a significant inhibitory effect against prostate tumor growth and metastasis. This study is designed to investigate the effects of HXL131 on the growth and metastasis of human PCa cell lines PC3 and its molecular mechanism through in vitro experiments combined with proteomics, molecular docking, and gene silencing. The in vitro results showed that HXL131 concentration dependently inhibited PC3 cell proliferation, induced apoptosis, arrested cell cycle at the G2/M phase, and inhibited cell migration capacity. A proteomic analysis and a Western blot showed that HXL131 up-regulated the expression of proliferation, apoptosis, cell cycle, and migration-related proteins CYR61, TIMP1, SOD2, IL6, SERPINE2, DUSP1, TNFSF9, OSMR, TNFRSF10D, and TNFRSF12A. Molecular docking, a cellular thermal shift assay (CETSA), and gene silencing showed that HXL131 had a strong binding affinity with DUSP1 and TNFSF9, which are important target genes for inhibiting the growth and metastasis of PC3 cells. This study demonstrates that HXL131 exhibited excellent anti-prostate cancer activity and inhibited the growth and metastasis of prostate cancer cells by regulating the expression of DUSP1 and TNFSF9. [ABSTRACT FROM AUTHOR]

Despite its wide application across arid land types, furrow irrigation is often associated with numerous environmental problems related to deep percolation, runoff, and soil erosion. In this study, a straightforward approach was proposed to achieve higher uniformity and reduce erosion. Here, the impacts that a moveable "plug" has on the behavior of irrigation water in the furrow were simulated using FLOW-3D and HYDRUS-2D, where three plug heights and two flow rates were set. The effect of inflow rate and plug height on the water advance, water level, cumulative infiltration in the furrow, and uniformity coefficient was determined. Results indicate that the plug was able to slow water velocity by approximately 60% in the furrow and increase the furrow advance time by 3–4 times; the water level was increased by nearly 10 cm compared with no plug. Moreover, an irrigation uniformity range of 90.18–99.22% was associated with this plugging. The addition of a plug in the furrow irrigation practices for smallholder farmers in developing countries demonstrates great potential in reducing the probability of erosion under large slopes and can effectively improve irrigation uniformity. [ABSTRACT FROM AUTHOR]

Background: The data on the relationship between statin use and clinical outcomes after intravenous thrombolysis (IVT) for acute ischemic stroke (AIS) are in controversy. Objective: This systematic review and meta-analysis aimed to evaluate the safety and efficacy of statins administered prior to onset and during hospitalization in patients with AIS treated with IVT. Methods: We searched PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials from inception until June 8, 2021. Comparative studies investigating statin effect on intracranial hemorrhage (ICH), functional outcomes, and mortality in adults with AIS treated with IVT were screened. Random-effect meta-analyses of odds ratios (ORs) with corresponding 95% confidence intervals (CIs) were performed. The protocol was registered in PROSPERO (CRD42021254919). Results: Twenty-two observational studies were included, which involved 17,554 patients. The pooled estimates showed that pre-stroke statin use was associated with a higher likelihood of symptomatic ICH (OR 1.31; 95% CI 1.07–1.59; p = 0.008) and any ICH (OR 1.21; 95% CI 1.03–1.43; p = 0.02). However, the pre-stroke statin use was not significantly associated with the 3-month mortality, 3-month favorable functional outcome (FFO, modified Rankin Scale [mRS] score 0–1), and 3-month functional independence (FI; mRS score 0–2). However, in-hospital statin use was associated with a reduced risk of symptomatic ICH (OR 0.46; 95% CI 0.21–1.00; p = 0.045), any ICH (OR 0.51; 95% CI 0.27–0.98; p = 0.04), and 3-month mortality (OR 0.42; 95% CI 0.29–0.62; p < 0.001) and an increased probability of 3-month FFO (OR 1.33; 95% CI 1.02–1.744; p = 0.04) and 3-month FI (OR 1.41; 95% C, 1.11–1.80; p = 0.005). Conclusions: The present systematic review and meta-analysis suggests that in-hospital statin use after IVT may be safe and may have a favorable impact on clinical outcomes, a finding not observed in studies restricted to patients with pre-stroke statin use. [ABSTRACT FROM AUTHOR]

In this article, we present a novel nonlinear vector network analyzer (NVNA) setup for generating and measuring digitally modulated signals at millimeter-wave test bands. The first difference between this setup and commercial NVNAs is the use of a user-defined multisine signal as the phase reference, in order to enable more precise synchronization and denser spectral grid. To achieve this, a single 12.5-GHz microwave source is used for generating both the digitally modulated test signal and the phase reference, which not only offers the clock to the arbitrary waveform generator for user-defined intermediate frequency (IF) waveform generation but also provides the local oscillator drive after frequency multiplication. The second novelty of this work is the use of pulsed-radio frequency (RF) signals for NVNA phase calibration. By replacing the digitally modulated IF waveform with pulsed-RF signals, tone-by-tone phase calibration on dense spectral grids can be easily achieved under the same synchronization scheme. The validity of the proposed NVNA setup (as well as its simplified modification) and the phase calibration method is verified at the fifth-generation (5G)-oriented millimeter-wave bands of 28, 39, 43, and 70 GHz in this work, where the EVM measurements of 64-QAM test signals with a wide range of symbol rates from 0.1 to 1.5625 GSymbol/s are demonstrated reachable in practice. [ABSTRACT FROM AUTHOR]

Introduction: Intracerebral hemorrhage (ICH) is the most fatal type of stroke worldwide. Herein, we aim to develop a predictive model based on computed tomography (CT) markers in an ICH cohort and validate it in another cohort. Methods: This retrospective observational cohort study was conducted in 3 medical centers in China. The values of CT markers, including hypodensities, hematoma density, blend sign, black hole sign, island sign, midline shift, baseline hematoma volume, and satellite sign, in predicting poor outcome were analyzed by logistic regression analysis. A nomogram was developed based on the results of multivariate logistic regression analysis in development cohort. Area under curve (AUC) and calibration plot were used to assess the accuracy of nomogram in this development cohort and validate in another cohort. Results: A total of 1,498 patients were included in this study. Multivariate logistic regression analysis indicated that hypodensities, black hole sign, island sign, midline shift, and baseline hematoma volume were independently associated with poor outcome in development cohort. The AUC was 0.75 (95% confidence interval [CI]: 0.73–0.76) in the internal validation with development cohort and 0.74 (95% CI: 0.72–0.75) in the external validation with validation cohort. The calibration plot in development and validation cohort indicated that the nomogram was well calibrated. Conclusions: CT markers of hypodensities, black hole sign, and island sign might predict poor outcome of ICH patients within 90 days. [ABSTRACT FROM AUTHOR]

In this paper, laser annealing is used to produce metal (Ag) nanoparticles as etching catalyst on a silicon surface, which enables controllable fabrication of large-scale nanohole array surface texturing without using a mask. Semispherical Ag nanoparticles with variable size and distribution are achievable by manipulating the laser annealing parameters and metal film thickness, and the underlying physics is clarified. The nanoholes array in silicon can then be realized by selective etching of silicon under Ag pattern. The optical characteristics suggest that the surface reflection can be significantly suppressed owing to the nanohole texturing, which is promising for thin film photovoltaic applications. [ABSTRACT FROM AUTHOR]

Background/Aims: Raddeanin A (RA), an active pharmacological ingredient from Anemone raddeana Regel, plays an important role in tumor suppression. In this study, we assessed the potentially therapeutic effect of RA on glioblastoma and its underlying mechanisms. Methods: Cell viability was examined using the MTT assay. Invasive and migratory capacities were examined using Transwell and wound healing assays. Apoptosis was determined by Hoechst staining, flow cytometry, DCFH-fluorescent probe and immunohistochemical staining. Autophagy was detected by transmission electron microscopy and western blotting. A U251 glioma xenograft model was established to evaluate the effect of RA in vivo. Results: The data demonstrated that RA inhibited viability, and abrogated the invasive/migratory abilities of glioblastoma cells. In addition, RA induced apoptosis by reactive oxygen species (ROS)/ Jun N-terminal kinase (JNK) signaling in glioblastoma. Conversely, the antioxidant N-Acetyl-L-cysteine (NAC) and pan-caspase inhibitor z-VAD-fmk attenuated RA-induced apoptosis by scavenging ROS and inactivating caspase-3. Furthermore, the inhibition of autophagy by 3-MA exacerbated apoptosis through ROS generation and JNK phosphorylation. In vivo, RA exhibited a curative effect on U251-derived xenografts in nude mice. Conclusions: The results of this study suggest that RA suppressed the growth of glioblastoma, thus serving as a promising and potential strategy for glioblastoma chemotherapy. [ABSTRACT FROM AUTHOR]

Solution-based nickel induced crystallization of amorphous silicon (a-Si) films was performed. The nickel solution was prepared by dissolving (CH3CO2)2Ni in deionized water and applied uniformly on a-Si films by low-cost ultrasonic spray pyrolysis method. Crystallization could be realized for a-Si films coated with a 0.2 M nickel solution and annealed at 500 °C. The effect of substrate temperature during nickel solution deposition was analyzed. Micro-Raman and x-ray diffraction measurement show that a-Si is fully crystallized at 550 °C for 7 h with a nickel concentration of 0.8 M. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [ABSTRACT FROM AUTHOR]

A vaccine derived from the outer membrane proteins of the Gram-negative bacterium Pseudomonas aeruginosa has been shown to have immune modulatory properties. An inactivated mutant strain of P. aeruginosa with mannose sensitive hemagglutinin fimbria (PA-MSHA) has been used for adjuvant therapy for malignant cancer. In this study, the growth of human hepatocellular carcinoma Hep G2 and BEL-7402 cells is inhibited by PA-MSHA, but not by mannose-cleaved PA-MSHA. PA-MSHA-treated cells arrested in the S phase of the cell cycle and underwent apoptosis. We hypothesize that apoptosis induced by treatment of Hep G2 and BEL-7402 cells with PA-MSHA is mediated by the mannose residues of PA-MSHA and is propagated through the extrinsic apoptosis pathway directly through caspase-8. These data provide mechanistic details for the potential application of PA-MSHA-based treatment of hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]

In this letter, we report light absorption enhancement in crystalline Si thin film-based solar cells through introducing low aspect-ratio hemispherical nanopit surface textures. Optical characteristics of the system are systematically investigated in terms of the structural parameters including nanopit diameter (D) and array periodicity (P) via simulation. An ultimate efficiency of ∼35.7% is predicted when both D and P are ∼700 nm, which is more than double of that (∼15.9%) of the flat Si film with the same thickness of 2 μm. An understanding on the light absorption enhancement is provided based on the optical processes in the subwavelength structures. [ABSTRACT FROM AUTHOR]