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Crocus sativus (saffron) is a globally autumn-flowering plant, and its stigmas are the most expensive spice and valuable herb medicine. Crocus specialized metabolites, crocins, are biosynthesized in distant species, Gardenia (eudicot) and Crocus (monocot), and the evolution of crocin biosynthesis remains poorly understood. With the chromosome-level Crocus genome assembly, we revealed that two rounds of lineage-specific whole genome triplication occurred, contributing important roles in the production of carotenoids and apocarotenoids. According to the kingdom-wide identification, phylogenetic analysis, and functional assays of carotenoid cleavage dioxygenases (CCDs), we deduced that the duplication, site positive selection, and neofunctionalization of Crocus-specific CCD2 from CCD1 members are responsible for the crocin biosynthesis. In addition, site mutation of CsCCD2 revealed the key amino acids, including I143, L146, R161, E181, T259, and S292 related to the catalytic activity of zeaxanthin cleavage. Our study provides important insights into the origin and evolution of plant specialized metabolites, which are derived by duplication events of biosynthetic genes.

In the theory of numerical semigroups, characterizing numerical semigroups in terms of pseudo-Frobenius numbers is one of the fundamental problems, which is very difficult to achieve in general. This article's main purpose is to answer this problem in the case of perfect numerical semigroups having a Maximal Embedding Dimension (MED).

Abstract Cepharanthine is a secondary metabolite isolated from Stephania. It has been reported that it has anti-conronaviruses activities including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Here, we assemble three Stephania genomes (S. japonica, S. yunnanensis, and S. cepharantha), propose the cepharanthine biosynthetic pathway, and assess the antiviral potential of compounds involved in the pathway. Among the three genomes, S. japonica has a near telomere-to-telomere assembly with one remaining gap, and S. cepharantha and S. yunnanensis have chromosome-level assemblies. Following by biosynthetic gene mining and metabolomics analysis, we identify seven cepharanthine analogs that have broad-spectrum anti-coronavirus activities, including SARS-CoV-2, Guangxi pangolin-CoV (GX_P2V), swine acute diarrhoea syndrome coronavirus (SADS-CoV), and porcine epidemic diarrhea virus (PEDV). We also show that two other genera, Nelumbo and Thalictrum, can produce cepharanthine analogs, and thus have the potential for antiviral compound discovery. Results generated from this study could accelerate broad-spectrum anti-coronavirus drug discovery.

Abstract Menispermaceae species, as early‐diverging eudicots, can synthesize valuable benzylisoquinoline alkaloids (BIAs) like bisbenzylisoquinoline alkaloids (bisBIAs) and sinomenines with a wide range of structural diversity. However, the evolutionary mechanisms responsible for their chemo‐diversity are not well understood. Here, a chromosome‐level genome assembly of Menispermum dauricum is presented and demonstrated the occurrence of two whole genome duplication (WGD) events that are shared by Ranunculales and specific to Menispermum, providing a model for understanding chromosomal evolution in early‐diverging eudicots. The biosynthetic pathway for diverse BIAs in M. dauricum is reconstructed by analyzing the transcriptome and metabolome. Additionally, five catalytic enzymes – one norcoclaurine synthase (NCS) and four cytochrome P450 monooxygenases (CYP450s) – from M. dauricum are responsible for the formation of the skeleton, hydroxylated modification, and C‐O/C‐C phenol coupling of BIAs. Notably, a novel leaf‐specific MdCYP80G10 enzyme that catalyzes C2′‐C4a phenol coupling of (S)‐reticuline into sinoacutine, the enantiomer of morphinan compounds, with predictable stereospecificity is discovered. Moreover, it is found that Menispermum‐specific CYP80 gene expansion, as well as tissue‐specific expression, has driven BIA diversity in Menispermaceae as compared to other Ranunculales species. This study sheds light on WGD occurrences in early‐diverging eudicots and the evolution of diverse BIA biosynthesis.

Using a combination of experimental and numerical methods, this study examines the stability of the slope of Waste Dump#1 in Ziluoyi Iron Mine. We conducted direct shear tests on soil samples taken from the waste dump, which provided important insights into slope stability. The tests identified key mechanical parameters, including an average cohesion of 4.80 kPa and an internal friction angle of 25.63°. By implementing GEO-SLOPE software, we could determine that the slope stability factor is 1.047, which is far from the required safety standards. To address this issue, we proposed an appropriate rectification strategy including the construction of safety platforms and reconfiguration of the slope structure. This approach effectively improved the slope stability factor to 1.219 and met the safety criteria. In addition, particle flow code (PFC) simulations were methodically performed to model the slope morphology and particle displacement before and after rectification. The obtained results revealed a remarkable reduction in sliding areas and particle displacement post-rectification, enhancing mine safety and efficiency. Our findings provide valuable insights into the application of combined experimental and numerical methods to assess and improve slope stability in open-pit mines, which will substantially contribute to the field of geotechnical engineering and mining safety.

The instability of geological slopes in mining environments poses a significant challenge to the safety and efficiency of operations. Waste Dump#2 at the Ziluoyi Iron Mine in China is a notable case study that highlights the challenges associated with sizable base slopes and large step heights. To address hidden hazards in the mine and the above issues, an inclusive investigation is carried out to examine the physical and mechanical properties of the soil–rock slope through indoor testing and analyze the deformation mechanisms of the slope using numerical simulations, taking various factors into account. The study reveals that the stability of Waste Dump#2 is deeply affected by weight, groundwater conditions, earthquake loading, and rainfall. To this end, the cohesion and internal friction parameters of the soil–rock slope are first determined through direct shear tests, which show a cohesion of 6.215 kPa at the top of the slope and an internal friction angle of 34.12°. By adopting GEO-SLOPE, 3D Mine, and AutoCAD software, stability calculations of the slope are performed, which give stability coefficients of 1.547 under normal conditions, 1.276 in rainfall, and 1.352 in seismic conditions. These results meet safety standards and ensure the safe and efficient operation of the mine.

Abstract Breast cancer is the leading cause of cancer-related deaths in women and remains a formidable therapeutic challenge. Mitochondria participate in a myriad of essential cellular processes, such as metabolism, and are becoming an ideal target for cancer therapy. Artemisinin and its derivatives have demonstrated multiple activities in the context of various cancers. Mitochondrial autophagy(mitophagy) is one of the important anti-tumor mechanisms of artemisinin drugs. However, the lack of specific tumor targeting ability limits the anti-tumor efficacy of artemisinin drugs. In this study, a GSH-sensitive artesunate smart conjugate (TPP-SS-ATS) was synthesized and liposomes (TPP-SS-ATS-LS) that target tumor cells and mitochondria were further prepared. The advantages of TPP-SS-ATS-LS targeting to the breast tumor were verified by in vivo and in vitro evaluations. In our study, the cytotoxicity was obviously enhanced in vitro and tumor growth inhibition rate was increased from 37.7% to 56.4% at equivalent artesunate dosage in breast cancer orthotopic implanted mice. Meanwhile, mitochondrial dysfunction, suppression of ATP production and respiratory capacity were detected in breast cancer cells. We further discovered that TPP-SS-ATS-LS inhibited tumor cells proliferation through mitophagy by regulating PHB2 and PINK1 expression. These results provide new research strategies for the development of new artemisinin-based anti-tumor drugs.

Abstract Cerebral malaria (CM) is a life-threatening neurological complication caused by Plasmodium falciparum. About 627,000 patients died of malaria in 2020. Currently, artemisinin and its derivatives are the front-line drugs used for the treatment of cerebral malaria. However, they cannot target the brain, which decreases their effectiveness. Therefore, increasing their ability to target the brain by the nano-delivery system with brain-targeted materials is of great significance for enhancing the effects of antimalarials and reducing CM mortality. This study used glucose transporter 1 (GLUT1) on the blood–brain barrier as a target for a synthesized cholesterol-undecanoic acid–glucose conjugate. The molecular dynamics simulation found that the structural fragment of glucose in the conjugate faced the outside the phospholipid bilayers, which was conducive to the recognition of brain-targeted liposomes by GLUT1. The fluorescence intensity of the brain-targeted liposomes (na-ATS/TMP@lipoBX) in the mouse brain was significantly higher than that of the non-targeted liposomes (na-ATS/TMP@lipo) in vivo (P

Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron- and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species (ROS) and inducing ferroptosis in activated hepatic stellate cells (HSCs). By using activity-based protein profiling (ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay (CETSA), we show that celastrol directly binds to peroxiredoxins (PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6, through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1 (HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2, PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis.

Abstract Background Sepsis involves life-threatening organ dysfunction and is caused by a dysregulated host response to infection. No specific therapies against sepsis have been reported. Celastrol (Cel) is a natural anti-inflammatory compound that shows potential against systemic inflammatory diseases. This study aimed to investigate the pharmacological activity and molecular mechanism of Cel in models of endotoxemia and sepsis. Methods We evaluated the anti-inflammatory efficacy of Cel against endotoxemia and sepsis in mice and macrophage cultures treated with lipopolysaccharide (LPS). We screened for potential protein targets of Cel using activity-based protein profiling (ABPP). Potential targets were validated using biophysical methods such as cellular thermal shift assays (CETSA) and surface plasmon resonance (SPR). Residues involved in Cel binding to target proteins were identified through point mutagenesis, and the functional effects of such binding were explored through gene knockdown. Results Cel protected mice from lethal endotoxemia and improved their survival with sepsis, and it significantly decreased the levels of pro-inflammatory cytokines in mice and macrophages treated with LPS (P

Damage deterioration of rocks in cold regions under seasonal changes and daily cycles of freezing and thawing generate a series of engineering geological problems. These problems will seriously affect the safe and efficient production of open-pit mines. In this paper, a freeze–thaw cycle test and uniaxial compression test considering the natural conditions of the slope were carried out. Mechanical properties and damage mechanisms of open-pit mine mudstone under freeze–thaw conditions were investigated based on nuclear magnetic resonance (NMR) technology. The test results show that the microscopic internal pore structure of mudstone was changed under the superimposed effect of freeze–thaw damage and hydration damage. The internal pore size of mudstone increased with the number of freeze–thaw cycles, while the average pore size of the natural mudstone test increased more. Macroscopically, the compressive strength and modulus of elasticity of mudstone varied linearly with the number of freezing cycles, and the compressive strength and modulus of elasticity showed a decreasing trend. The strain-softening characteristics of mudstone samples were significant for more freeze–thaw cycles. The study explains the microscopic causes of mudstone deterioration in open-pit mines in cold regions and offers guidance for solving engineering disasters caused by mudstone deterioration.

In the process of mining and transportation, the temporary non-hardened mine-road structure is mainly a mixture of soil and stone, which very easily produces dust hazards via crushing and wind transportation. Geopolymers can be used in the road’s soil and stone mixture so that the road reaches certain strength requirements in line with the short-term use of the mine. However, in alpine open-pit coal mines, which are subject to the influence of weather changes, freezing and thawing phenomena will affect the long-term use of the road and its normal and safe operation. An open-pit coal mine in Xinjiang, China, was chosen as the research object of alpine open-pit coal mines. Using the method of indoor testing, different freeze–thaw freezing temperatures, different numbers of cycles, changes in the mechanical properties of the mine-road materials, and microscopic changes were studied. From the experimental results, it was determined that with a reduction in the freeze–thaw freezing temperature, the specimen strength declines after stabilizing, and with an increase in the number of freeze–thaw cycles, the specimen strength exhibits a linear decline. The specimen’s internal structure gradually changed from dense to loose; the fracture mode changed from toughness fractures to crystal fractures after the removal of the maximum load reduction. The uniaxial compressive strength was reduced to 61%; the tensile strength was reduced to 49%. The fracture zone of the specimen was analyzed using energy spectra, and the main elements changed from O (57.19%), Si (17.07%), and Al (12.19%) without freezing and thawing to O (49.76%), Si (15.70%) and Ca (11.09%) after freezing and thawing.

Abstract In the Internet age, emotions exist in cyberspace and geospatial space, and social media is the mapping from geospatial space to cyberspace. However, most previous studies pay less attention to the multidimensional and spatiotemporal characteristics of emotion. We obtained 211,526 Sina Weibo data with geographic locations and trained an emotion classification model by combining the Bidirectional Encoder Representation from Transformers (BERT) model and a convolutional neural network to calculate the emotional tendency of each Weibo. Then, the topic of the hot spots in Nanchang City was detected through a word shift graph, and the temporal and spatial change characteristics of the Weibo emotions were analyzed at the grid-scale. The results of our research show that Weibo’s overall emotion tendencies are mainly positive. The spatial distribution of the urban emotions is extremely uneven, and the hot spots of a single emotion are mainly distributed around the city. In general, the intensity of the temporal and spatial changes in emotions in the cities is relatively high. Specifically, from day to night, the city exhibits a pattern of high in the east and low in the west. From working days to weekends, the model exhibits a low center and a four-week high. These results reveal the temporal and spatial distribution characteristics of the Weibo emotions in the city and provide auxiliary support for analyzing the happiness of residents in the city and guiding urban management and planning.

Abstract Background Trimetazidine (TMZ) pretreatment protects cardiomyocytes during cardiac surgery. TMZ may protect elderly patients with ischaemic heart disease (IHD) undergoing non-cardiac surgery. Methods This was a randomized, double-blind, placebo-controlled trial (registration #ChiCTR1900025018) of patients with IHD scheduled to undergo non-cardiac surgery at Shenzhen People’s Hospital (Shenzhen, Guangdong Province, China) between June 2014 and September 2015, randomized to 60 mg TMZ or placebo 12 h before surgery. The primary endpoint was the occurrence of in-hospital cardiovascular events. The secondary endpoints were myocardial ischaemia on five-lead electrocardiogram (cECG), cardiac troponin I (cTnI) elevation, cardiac death, acute coronary events, heart failure, and arrhythmia requiring treatments. Results Compared with the placebo group, the TMZ group showed a lower occurrence of in-hospital cardiovascular events (primary endpoint, 20.0% vs. 37.5%, P = 0.02), myocardial ischaemia (15.0% vs. 32.5%, P

Background and ObjectiveThere is keen interest in better understanding the impacts of alpha-linolenic acid (ALA), a plant-derived n-3 fatty acid, in ameliorating the development of cancer; however, results of several prospective cohorts present an inconsistent association between ALA intake and the incident colorectal cancer (CRC). We aimed to investigate the summary association of dietary intake and biomarkers of ALA with CRC risk based on the prospective cohorts.MethodsPertinent prospective cohorts were identified in Cochrane Library, PubMed, and EMBASE from inception to February 2022. Study-specific risk ratios (RRs) with 95% confidence intervals (CIs) for comparing the top with the bottom quartiles of ALA levels were combined using a random-effects model. Nonlinear dose-response relationships of ALA levels in diet and blood with CRC risk were assessed using the restricted cubic spline models, respectively.ResultsOver the duration of follow-up with a median of 9.3 years ranging from 1 to 28 years, 12,239 CRC cases occurred among 861,725 participants from 15 cohorts (11 studies on diet and 5 studies on biomarkers including 4 on blood and 1 on adipose tissue). The summary RR was 1.03 (95% CI: 0.97, 1.10; I2: 0.00%) for dietary intake and 0.83 (95% CI: 0.69, 0.99; I2: 0.00%) for biomarker. Each 0.1% increase in the levels of ALA in blood was associated with a 10% reduction in risk of CRC (summary RR: 0.90, 95% CI: 0.80, 0.99; I2: 38.60%), whereas no significant dose-response association was found between dietary intake of ALA and the incident CRC (p for non-linearity = 0.18; p for linearity = 0.24).ConclusionsBlood levels of ALA were inversely and linearly associated with the risk of CRC, which suggested that increased intake of ALA to improve circulating levels was beneficial for CRC prevention.

Once an human immunodeficiency virus (HIV)-infected individual enters the onset period, a variety of opportunistic infections may occur, affecting various systems and organs throughout the body, due to the considerable reduction in the body’s immune function. The objectives of this study were to explore the relationship between immune status and microbial communities in the lungs of individuals with HIV infection. A total of 88 patients with lung disease [80 (91%) HIV-positive and 8 (9%) HIV-negative] were enrolled in our study between January and July 2018, and 88 bronchoalveolar lavage fluid (BALF) samples were obtained during bronchoscopy. In this cross-sectional study, we investigated differences in the pulmonary microbiome of patients with HIV who had different immune statuses. The diversity of bacteria in the lungs of HIV-positive individuals was lower than that in HIV-negative individuals (p < 0.05). There was a significant difference in the composition and distribution of bacteria and fungi between the HIV-positive and HIV-negative groups (p < 0.01). The number of fungal species in the BALF of HIV-positive patients was higher than in HIV-negative patients. The diversity of bacteria and fungi in the BALF of HIV-positive patients increased with decreasing CD4 T-cell counts. Linear regression analysis showed that Pneumocystis (R2 = 6.4e−03, p < 0.05), Cryptosphaeria (R2 = 7.2e−01, p < 0.05), Candida (R2 = 3.9e−02, p < 0.05), and Trichosporon (R2 = 7.7e−01, p < 0.05) were negatively correlated with CD4 counts (F-test, p < 0.05). The samples collected from HIV-positive patients exhibited a different pattern relative to those from the HIV-negative group. Differences in host immune status cause differences in the diversity and structure of lower respiratory tract microorganisms.

In this paper, we study porous media equation $u_{t}=\Delta u^{m}-u^{p}$ with nonlinear boundary condition $\frac{\partial u}{\partial\nu}=ku^{q}$. We determine some sufficient conditions for the occurrence of finite time blow-up or global existence. Moreover, lower and upper bounds for blow-up time are also derived by using various inequality techniques.

An “on-off-on” type photoelectrochemical (PEC) aptamer sensor for prostate-specific antigen (PSA) detection is constructed based on nanocomposite materials obtained from CdS functionalized titanium dioxide (CdS/TiO2 NPs). Firstly, due to high photocurrent signal of the CdS/TiO2 NPs, it can achieve the initial signal “on” state to improve the stability of the sensing platform. Then, AuNPs, as the energy acceptor of CdS, are used to label PSA aptamers to obtain signal “off” state through energy resonance transfer (RET). Finally, in the process of detecting PSA, part of the AuNPs-modified aptamer detaches from the electrode surface, causing the sensor signal to “on” state again. This stepped energy level structure formed by the coupling of CdS and TiO2 NPs can broaden the light absorption range, enhance electron-hole separation, and lay a good foundation for the signal “on-off-on” strategy. The method on RET to realize signal quenching and opening significantly improves the sensitivity of the PEC sensor. Based on this innovative “on-off-on” design, this PEC aptamer sensor has high sensitivity to PSA, and its linear detection range (LDR) is 0.0005–50 ng mL−1. The limit of detection (LOD) is 0.00017 ng mL−1. This constructed PEC sensor also has good detection performances in actual human serum sample detection. And this study based on RET can also provide a new strategy for the detection of other biomarkers.

DNA damage and antioxidant activities of sour meat peptides were evaluated using chemiluminescence and spectroscopic methods. All sour meat peptides exhibited DNA damage protection and free radical scavenging activities against DPPH radicals, hydroxyl radicals and superoxide anions in a dose-dependent manner, and showed the strongest inhibition of hydroxyl radicals and the weakest inhibition of superoxide anion radicals. The IC50 values of sour meat peptides from inoculating fermentation were 2.11, 4.23, 0.097 and 9.85 mg/mL for the inhibition of DNA damage and scavenging capacities of DPPH radicals, hydroxyl radicals and superoxide anion radicals, respectively, and were significantly lower than those of traditional fermentation (P

Background: Alzheimer’s disease (AD) is the most common form of dementia worldwide. Previous studies have reported that sevoflurane, a frequently used anesthetic, can induce cognitive impairment in preclinical and clinical settings. However, the mechanism underlying the development of this neurotoxicity is currently unclear.Methods: Seven-month-old APP/PS1 mice were placed in an anesthesia induction box containing 3% sevoflurane in 100% O2 for 6 h, while BV2 cells were cultured with 4% sevoflurane for 6 h. Pyroptosis and tau protein expression in excised hippocampus tissues and cells were measured using Western blotting and immunofluorescence assay. Caspase-1 and NLRP3 were knocked out in BV2 microglia using CRISPR/Cas9 technology to determine whether they mediate the effects induced by sevoflurane.Results: Sevoflurane directly activated caspase-1 to induce pyroptosis in the mouse model of AD via NLRP3 and AIM2 activation. In addition, sevoflurane mediated cleavage of gasdermin D (GSDMD) but not gasdermin E (GSDME), promoted the biosynthesis of downstream interleukin-1β and interleukin-18, and increased β-amyloid (Aβ) deposition and tau phosphorylation. The nontoxic caspase-1 small-molecule inhibitor VX-765 significantly inhibited this activation process in microglia, while NLRP3 deletion suppressed sevoflurane-induced caspase-1 cleavage and subsequently pyroptosis, as well as tau pathology. Furthermore, silencing caspase-1 alleviated the sevoflurane-induced release of IL-1β and IL-18 and inhibited tau-related enzymes in microglia.Conclusion: This study is the first to report that clinical doses of sevoflurane aggravate the progression of AD via the NLRP3/caspase-1/GSDMD axis. Collectively, our findings elucidate the crucial mechanisms of NLRP3/caspase-1 in pyroptosis and tau pathogenesis induced by sevoflurane and suggest that VX-765 could represent a novel therapeutic intervention for treating AD.

Air pollution is closely associated with human health and the economy. Therefore, it is important to understand variations in the spatiotemporal and sectoral emission distributions of major air pollutants and their drivers. The policies (APAPPC) promulgated by China in 2013 have also achieved remarkable results. Rate of change, trend analysis, and a geographically and temporally weighted regression model were used to study the effects of socioeconomic factors on NOx, SO2, and dust emissions in China during 2011–2017. During the study period, annual average emissions of NOx, SO2, and dust decreased by 11.45, 13.42, and 4.82 Mt (−47.64, −60.53, and −39.05%), respectively. Pollutant emissions were concentrated in North China, with Shandong and Hebei provinces exhibiting the highest NOx and SO2 and dust emissions, respectively. Pollutant emissions from the power and industrial sectors were mainly distributed in East (27.08 and 28.00%, respectively) and North China (23.57 and 20.04%, respectively), whereas emissions from the residential sector were mainly concentrated in North (22.48%) and Southwest China (20.07%). Pollutant emissions were positively correlated with electricity generation, urban population density, urban green spaces, private car ownership, the secondary industry as a share of regional GDP, and steel production and negatively correlated with disposable income and gross construction output. Per capita disposable income was the dominant driving factor.

A diet high in sodium chloride (NaCl) can affect renal function damage and increase urinary calcium excretion, leading to bone loss. in renal tubules, Na-Cl co-transporter (NCC) and chloride channel 5 (CLC-5) are involved in regulating urinary calcium excretion. In addition, some cytokines, such as Bone morphogenetic protein 7 (BMP-7) and 1α-hydroxylase, are synthesized by renal tubules, which target on bone and play important roles on bone metabolism. However, the specific mechanisms between NaCl and these ion channels or cytokines still need investigations from many aspects. This study, in culture normal rat renal tubular epithelial NRK-52E cells, showed that high concentrations of NaCl significantly inhibited the cell viability and increased the cell apoptosis. High concentration of NaCl reduce bone mineral density (BMD), as demonstrated by the significantly increased mRNA and protein levels of NCC and osteopontin (OPN), but decreased the levels of CLC-5, BMP-7, and 1α-hydroxylase. In addition, we found that ovariectomized (OVX) rats on a high-salt diet for 12 weeks had altered levels of these indices in the renal cortices. Moreover, the BMD in fourth and fifth lumbar vertebra (LV4 and 5) and femurs were significantly decreased and bone microstructure was destroyed of these rats. We also demonstrated that high concentration of NaCl enhanced the inhibition of these cytokines which is beneficial to increase BMD, induced by modulating ion channels NCC and CLC-5. In conclusion, our results indicate that high concentration of NaCl reduce BMD by regulating ion channels NCC and CLC-5.

This study presents different land saving and loss reducing schemes. Comprehensively compare the economic benefits of the schemes. It is shown that the optimal scheme can recover tons of coal resources on the south slope, creating significant economic benefits. Numerical simulation was utilized to analyze the changes of slope stability, deformation, and shear strain increment in the process of land saving and loss reducing. Results found that the slope stability decreases rapidly, and the toe of the slope has to be internally discharged in time to compress the slope. Timely follow-up of in-slope rows can improve slope stability. The position of the slope shear exit changes with the increase of the distance of the inner row, and its stability increases gradually. When the distance of the lower inner row exceeds 120 m, the increase of the internal row distance has little influence on the slope stability. When the inner distance exceeds 60 m in the process of internal dumping of upper platform, the position of potential sliding plane gradually changes from the lower dump to the upper and lower dump with the increase of inner dumping distance. It shows that the stability of the dump will be damaged if the upper dump continues to be discharged. Therefore, the distance between the upper and lower dump sites is more important than 60 m. The stability of the south slope is good in the process of mining and internal drainage, and the overall stability of the south slope is controllable, based on slope radar monitoring.

We propose a scheme to generate remote asymmetric Einstein-Podolsky-Rosen (EPR) steering of two magnon modes in a hybrid ferromagnet-superconductor system. In our scheme, the indirect coherent coupling between two distant magnons and a Δ-type superconducting atom is established via exchange of virtual photons in two dispersive cavities. As a consequence, the artificial atom acting as a reservoir can produce asymmetrical magnon-magnon steering via a single dissipation channel. Interestingly, we find that the steering directivity is determined by cooling either Bogoliubov mode selectively rather than increasing the extra noise as in conventional schemes. This provides a novel idea to prepare remote EPR steering in solid devices, which may find potential applications in quantum information processing.

This paper deals with a parabolic-elliptic chemotaxis-growth system with nonlinear sensitivity \begin{equation*}\label{1a} \begin{cases} u_t=\Delta u-\chi\nabla\cdot(\psi(u)\nabla v)+f(u), &(x,t)\in \Omega\times (0,\infty), \\ 0=\Delta v-v+g(u), &(x,t)\in \Omega\times (0,\infty), \end{cases} \end{equation*} under homogeneous Neumann boundary conditions in a smooth bounded domain $\Omega\subset \mathbb{R}^{n}$ $(n\geq1)$, where $\chi>0$, the chemotactic sensitivity $\psi(u)\leq(u+1)^{q}$ with $q>0$, $g(u)\leq(u+1)^{l}$ with $l\in \mathbb{R}$ and $f(u)$ is a logistic source. The main goal of this paper is to extend a previous result on global boundedness by Zheng et al. [J. Math. Anal. Appl. 424(2015), 509–522] under the condition that $1\leq q+l

Salt stress is one of the abiotic factors that causes adverse effects in plants and there is an urgent need to detect salt stress in plants as early as possible. Multicolor fluorescence imaging, as a powerful tool in plant phenotyping, can provide information about primary and secondary metabolism in plants to detect the responses of the plants exposed to stress in the early stage. The purpose of this study was to evaluate the potential of multicolor fluorescence imaging’s application in the early detection of salt stress in plants. In this study, the measurements were conducted on Arabidopsis and the multicolor fluorescence images were acquired at 440, 520, 690, and 740 nm with a self-developed imaging system consisting of a UV light-emitting diode (LED) panel for an excitation at 365 nm, a charge coupled device (CCD) camera, interference filters, and a computer. We developed a classification method using the imaging analysis of multicolor fluorescence based on principal component analysis (PCA) and a support vector machine (SVM). The results showed that the four principal fluorescence feature combinations were the ideal indicators as the inputs of the SVM model, and the classification accuracies of the control and salt-stress treatment at 5 days and 9 days were 92.65% and 98.53%, respectively. The results indicated that multicolor fluorescence imaging combined with PCA and SVM could act as a tool for early detection in salt-stressed plants.

Panax ginseng C.A. Meyer (Araliaceae), a popular tonic and dietetic herbal medicine, has been traditionally prescribed in China and other countries to treat affective disorders. The medicinal parts of ginseng, the roots and flower buds, have become increasingly popular as dietary supplements due to the current holistic healthcare trend. We have investigated for the first time the antidepressive actions of the different medicinal parts, namely, the main roots, fibrous roots, and flower buds (in water extract and powder), of garden-cultivated ginseng through behavioral and drug-induced tests in mice. The water extracts, but not the powders of ginseng fibrous roots, flower buds, and main roots (1.5 g of crude drug per kilogram, p.o.), significantly reduced the immobility time in the forced swim test (FST) and tail suspension test (TST); moreover, the water extracts enhanced the 5-hydroxytryptophan (5-HTP)-induced head-twitch response and antagonized the action of reserpine in the mouse. We then explored the antidepressive mechanism of action of the ginsenoside Rb1 (Rb1) related to the brain-derived neurotrophic factor (BDNF) and its downstream proteins in mice exposed to chronic unpredictable mild stress (CUMS). Treatment with Rb1 (20 mg/kg, p.o.) for 21 days significantly attenuated the CUMS-induced decrease in the activities of BDNF, tropomyosin-related kinase B (TrkB), protein kinase B (AKT), extracellular regulatory protein kinase (ERK), and cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) in the mouse hippocampal CA3 region and prefrontal cortex (PFC). Interestingly, treatment with the novel TrkB antagonist ANA-12 (0.5 mg/kg, i.p.) did not alter the level of BDNF but significantly blocked the antidepressive effects of Rb1 on proteins downstream of BDNF in CUMS-treated mice. These results suggest that BDNF–TrkB–CREB signaling may be involved in the antidepressive mechanism of the action of Rb1.

Spectral imaging is a promising technique for detecting the quality of rice seeds. However, the high cost of the system has limited it to more practical applications. The study was aimed to develop a low-cost narrow band multispectral imaging system for detecting rice false smut (RFS) in rice seeds. Two different cultivars of rice seeds were artificially inoculated with RFS. Results have demonstrated that spectral features at 460, 520, 660, 740, 850, and 940 nm were well linked to the RFS. It achieved an overall accuracy of 98.7% with a false negative rate of 3.2% for Zheliang, and 91.4% with 6.7% for Xiushui, respectively, using the least squares-support vector machine. Moreover, the robustness of the model was validated through transferring the model of Zheliang to Xiushui with the overall accuracy of 90.3% and false negative rate of 7.8%. These results demonstrate the feasibility of the developed system for RFS identification with a low detecting cost.

This paper proposes an adaptive absolute ego-motion estimation method using wearable visual-inertial sensors for indoor positioning. We introduce a wearable visual-inertial device to estimate not only the camera ego-motion, but also the 3D motion of the moving object in dynamic environments. Firstly, a novel method dynamic scene segmentation is proposed using two visual geometry constraints with the help of inertial sensors. Moreover, this paper introduces a concept of “virtual camera” to consider the motion area related to each moving object as if a static object were viewed by a “virtual camera”. We therefore derive the 3D moving object’s motion from the motions for the real and virtual camera because the virtual camera’s motion is actually the combined motion of both the real camera and the moving object. In addition, a multi-rate linear Kalman-filter (MR-LKF) as our previous work was selected to solve both the problem of scale ambiguity in monocular camera tracking and the different sampling frequencies of visual and inertial sensors. The performance of the proposed method is evaluated by simulation studies and practical experiments performed in both static and dynamic environments. The results show the method’s robustness and effectiveness compared with the results from a Pioneer robot as the ground truth.