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The circadian clock regulates the behavior, physiology, and metabolism of mammals, and these characteristics, such as sleep-wake cycles, exercise capacity, and hormone levels, exhibit circadian rhythms. Light signaling is the main stimulator of the mammalian circadian system. The photoperiod regulates the reproductive cycle of seasonal breeding animals, and the circadian clock plays a pivotal role in this process. However, the role of the clock in coordinating animal behavior and physiology in response to photoperiodic changes needs further investigation. The present study investigated the changes and correlation of behavioral activities, physiological indicators, and gene expression in female striped hamsters (Cricetulus barabensis) within 24 h under a 12L:12D photoperiod. We found that the daily rhythms of sleep-wake and open field were significant in hamsters. The expression of clock genes, melatonin receptor genes, and genes involved in general metabolism oscillated significantly in central and peripheral tissues (brain, hypothalamus, liver, ovary, and thymus) and was significantly associated with behavior and physiology. Our results revealed that the neuroendocrine system regulated the rhythmicity of behavior and physiology, and central and peripheral clock genes (Bmal1, Clock, Per1, Per2, Cry1, and Cry2), melatonin receptor genes (MT1, MT2, and GPR50), and metabolizing genes (SIRT1, FGF21, and PPARα) played important roles. Our results suggest that central and peripheral circadian clocks, melatonin receptors, and genes involved in general metabolism may play key roles in maintaining circadian behavior and metabolic homeostasis in striped hamsters. Our results may have important implication for rodent pest control.

Intracellular zinc accumulation has been shown to be associated with neuronal death after cerebral ischemia. However, the mechanism of zinc accumulation leading to neuronal death in ischemia/reperfusion (I/R) is still unclear. Intracellular zinc signals are required for the production of proinflammatory cytokines. The present study investigated whether intracellular accumulated zinc aggravates I/R injury through inflammatory response, and inflammation-mediated neuronal apoptosis. Male Sprague–Dawley rats were treated with vehicle or zinc chelator TPEN 15 mg/kg before a 90-min middle cerebral artery occlusion (MCAO). The expressions of proinflammatory cytokines TNF-α, IL-6, NF-κB p65, and NF-κB inhibitory protein IκB-α, as well as anti-inflammatory cytokine IL-10 were assessed at 6 or 24 h after reperfusion. Our results demonstrated that the expression of TNF-α, IL-6, and NF-κB p65 increased after reperfusion, while the expression of IκB-α and IL-10 decreased, suggesting that cerebral ischemia triggers inflammatory response. Furthermore, TNF-α, NF-κB p65, and IL-10 were all colocalized with the neuron-specific nuclear protein (NeuN), suggesting that the ischemia-induced inflammatory response occurs in neurons. Moreover, TNF-α was also colocalized with the zinc-specific dyes Newport Green (NG), suggesting that intracellular accumulated zinc might be associated with neuronal inflammation following cerebral I/R. Chelating zinc with TPEN reversed the expression of TNF-α, NF-κB p65, IκB-α, IL-6, and IL-10 in ischemic rats. Besides, IL-6-positive cells were colocalized with TUNEL-positive cells in the ischemic penumbra of MCAO rats at 24 h after reperfusion, indicating that zinc accumulation following I/R might induce inflammation and inflammation-associated neuronal apoptosis. Taken together, this study demonstrates that excessive zinc activates inflammation and that the brain injury caused by zinc accumulation is at least partially due to specific neuronal apoptosis induced by inflammation, which may provide an important mechanism of cerebral I/R injury.

Conceptual design is a key link in the process of complex product design, and it is very important to select the appropriate design scheme; however, there are many types and inaccuracies of the evaluation data, and there is a problem of mutual influence between the evaluation criteria, which leads to unreliable decision making of the optimal solution. In order to solve this problem, a decision-making method based on intuitionistic fuzzy sets (IFS) and prospect theory is proposed. This method can be used for symmetric and asymmetric evaluation data. The evaluation data are classified according to different expression types and unified using intuitionistic fuzzy numbers. The intuitionistic fuzzy prospect value of decision information is calculated using prospect theory, and the prospect transformation of decision information is completed. At the same time, the Gray Relational Analysis (GRA) method and the Criteria Importance Though Intercriteria Correlation (CRITIC) method are used to calculate the subjective and objective weights of the technical and economic evaluation indexes of the product, and the combination weights are given; then, based on the evidence theory, the basic probability distribution of the evidence chain of all conceptual design schemes is synthesized, and the comprehensive prospect evaluation results of the schemes are obtained to complete the optimization of the conceptual design schemes. Finally, the effectiveness of the proposed method is verified by the conceptual design of the chip removal system of the deep hole machining machine tool. This work provides a promising method for decision makers to optimize the design scheme and provides insights into multi-objective decision-making problems.

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Background: To further optimize the mechanochemical pretreatment process, a combined wet alkaline mechanical pretreatment of corn stover was proposed with a short time and less chemical consumption at room temperature. Results: The combined alkaline mechanical pretreatment signifcantly enhanced enzymatic hydrolysis resulting a highest glucose yield (YG) of 91.9% with 3% NaOH and ball milling (BM) for 10 min. At this optimal condition, 44.4% lignin was removed and major portion of cellulose was retained (86.6%). The prehydrolysate contained by-products such as monosaccharides, oligosaccharides, acetic acid, and lignin but no furfural and 5-HMF. The alkaline concentration showed a signifcant impact on glucose yield, while the BM time was less important. Quantitative correlation analysis showed that YG (%)=0.68×BM time (min)+19.27×NaOH concentration (%)+13.71 (R²=0.85), YG=6.35×glucan content−231.84 (R²=0.84), and YG= −14.22×lignin content+282.70 (R²=0.87). Conclusion: The combined wet alkaline mechanical pretreatment at room temperature had a boosting efect on the yield of enzymatic hydrolysis with short treatment time and less chemical consumption. The impact of the physical and chemical properties of corn stover pretreated with diferent BM times and/or diferent NaOH concentrations on the subsequent enzymatic hydrolysis was investigated, which would be benefcial to illustrate the efective mechanism of the mechanochemical pretreatment method. [ABSTRACT FROM AUTHOR]

Background: Cutaneous intravascular NK/T cell lymphoma (CIVNKTC) is a rare form of extranodal non-Hodgkin's lymphoma with its unique histological and immunophenotypic characteristics. Methods: We report a case presenting with an over 2-month history of nodules on the extremities and trunk with intermittent fever. Skin biopsy was taken from the patient and a histopathological examination was made from the material. Results: The histopathological examination showed some expanding vessels filled with atypical lymphoid cells in the dermis and subcutaneous tissue. The tumor cells had large nuclei and one or two small nucleoli, with the expression of CD3, cytotoxic protein, and Epstein-Barr virus (EBV)-encoded messenger RNAs (EBER), and without the expression of tumor cytokeratin (CK), CD20, CD79A, CD4, and CD8. After being diagnosed as CIVNKTC and treated with a CHOP regimen 6 times, the patient died of this disease 1 year later. Conclusions: The clinical course is dangerous and the prognosis is extremely poor. [ABSTRACT FROM AUTHOR]

Anaerobic digestion (AD) prepared with the biogas industry can be utilised to adsorb organic matter in wastewater for realising the resource utilisation of AD. The low-temperature dry cattle manure-derived anaerobic digestion (CMAD) is a potential method for preparing adsorbents. Slow pyrolytic carbon (CMSP) and fast pyrolytic carbon (CMFP) are prepared to compare the adsorption capacity of CMAD. This study conducts the characteristic tests, adsorption experiments and isotherm and kinetics analyses of these samples. Results show that the surface functional groups of CMAD are abundant, and the surface area is small. The adsorptions of Methylene blue (MB) on CMSP, CMFP and CMAD reach a slow adsorption process at 240, 360 and 120 min, respectively. The maximum removal amounts by CMSP, CMFP and CMAD are 96.88%, 53.03% and 95.72% for MB. The adsorption of MB and Congo red (CR) by CMAD appears as a heterogeneous monolayer on the basis of adsorption isotherm and kinetics. The adsorptions of MB and CR by CMAD are chemical and physical adsorptions, respectively. CMAD can be utilised as an adsorbent due to its rich functional groups. In the meantime, its adsorption performance can be largely enhanced by improving its specific surface area. [ABSTRACT FROM AUTHOR]

Predictive analysis using publicly available yeast functional genomics and proteomics data suggests that many more proteins may be involved in biogenesis of ribonucleoproteins than are currently known. Using a microarray that monitors abundance and processing of noncoding RNAs, we analyzed 468 yeast strains carrying mutations in protein-coding genes, most of which have not previously been associated with RNA or RNP synthesis. Many strains mutated in uncharacterized genes displayed aberrant noncoding RNA profiles. Ten factors involved in noncoding RNA biogenesis were verified by further experimentation, including a protein required for 20S pre-rRNA processing (Tsr2p), a protein associated with the nuclear exosome (Lrp1p), and a factor required for box C/D snoRNA accumulation (Bcd1p). These data present a global view of yeast noncoding RNA processing and confirm that many currently uncharacterized yeast proteins are involved in biogenesis of noncoding RNA. [Copyright &y& Elsevier]