Search

Abstract In the existing domain adaptation-based bearing fault diagnosis methods, the data difference between the source domain and the target domain is not obvious. Besides, parameters of target domain feature extractor gradually approach that of source domain feature extractor to cheat discriminator which results in similar feature distribution of source domain and target domain. These issues make it difficult for the domain adaptation-based bearing fault diagnosis methods to achieve satisfactory performance. An unsupervised domain adaptive bearing fault diagnosis method based on maximum domain discrepancy (UDA-BFD-MDD) is proposed in this paper. In UDA-BFD-MDD, maximum domain discrepancy is exploited to maximize the feature difference between the source domain and target domain, while the output feature of target domain feature extractor can cheat the discriminator. The performance of UDA-BFD-MDD is verified through comprehensive experiments using the bearing dataset of Case Western Reserve University. The experimental results demonstrate that UDA-BFD-MDD is more stable during training process and can achieve higher accuracy rate.

This study focuses on monitoring the deformation of the shallow unsymmetrical section of a super-large-span tunnel portal relying on the newly built Shimentangshan Tunnel, and through numerical simulations, the construction sequence and drift ratios were optimized to address challenges related to the stability of surrounding rock and structure. The findings indicate that employing the double-side drift method results in a maximum settlement value of 107.0 mm and a maximum convergence value of 108.8 mm, leading to larger deformations. Excavating the shallow buried side first followed by the deep buried side proves beneficial for deformation control of the support structure and effectively limits damage to the surrounding rock. A drift ratio of 0.3 ensures optimal support structure security and stability. Considering both structural deformation and surrounding rock damage, a ratio between 0.25 and 0.35 for the drifts is recommended. Taking into account construction efficiency and economic benefits, a construction plan for the shallow buried unsymmetrical section at the portal of super-large-span tunnels is proposed.

Abstract The performance of zeolite membrane supports directly determines the synthesis performance and industrial application of zeolite membranes. In this study, a novel and high permeation SiC supports for NaA zeolite membrane were prepared by carrying out in situ reaction bonding at high temperature in the air atmosphere to coat the SiC particles with aluminum sol. The effects of process parameters on the formation of porous structure of SiC supports were systematically investigated, and the underlying mechanism was explored. During the sintering process, high-temperature Al2O3 from aluminum sol reacted with SiO2 generated by oxidation of the surface of SiC particles to form mullite, which exhibited excellent chemical stability and improved the mechanical properties and chemical stability of the supports. The porous SiC supports for zeolite membrane displayed obvious three-dimensional connected pore structure, with smooth inner surface of the pores and obvious connection neck between the particles. The porous SiC supports for zeolite membrane showed good in situ mullite bonding between the particles, and high permeability. It has a porosity of 36.3%, an average pore size of 1.42 µm and a bending strength of 32.4 MPa. All these figures indicate good comprehensive properties that are consistent with the rigorous requirements of zeolite membrane synthesis and industrial application.

The IEEE 802.1 time sensitive networking working group has recently standardized the time aware shaper (TAS). The TAS provides deterministic latency guarantees but requires tight time synchronization in all network switches. This paper thoroughly evaluates the mean and maximum packet delays and packet losses of the TAS for a typical industrial control ring network for random (sporadic) and for periodic traffic. We propose and evaluate adaptive bandwidth sharing and adaptive slotted window mechanisms to make TAS adaptive to traffic fluctuations. This paper further evaluates the asynchronous traffic shaper (ATS), which has been proposed to provide low latency network service without the need for time synchronization in network nodes. Our evaluations indicate that TAS with proper configurations, e.g., accurate and precise gating schedules, generally achieves the specified latency bounds for both sporadic and periodic traffic. In contrast, ATS performs relatively well for sporadic traffic; but struggles for moderate to high loads of periodic traffic.

Abstract Wireless sensor nodes in wireless sensor networks are generally battery powered and it is usually hard to change their batteries. Therefore, energy saving has always been the basic critical problem of wireless sensor networks. In recent years, network coding has become a promising technology to improve network throughput, reduce transmission number, and save energy, which has great potential to solve the energy efficiency problem of wireless sensor networks. In addition, some network coding aware routings have been proposed. However, the network coding condition of existing network coding aware routings may experience the problem of false-coding effect in some scenarios, and usually neglect node energy, which greatly influences the energy efficiency performance. Therefore, existing network coding aware routings are not suitable for wireless sensor networks. This paper proposes network coding aware energy efficient routing (NAER) for wireless sensor networks. In NAER, universal network coding condition is presented and proved to avoid false-coding problem. Besides, combined with the coverage control and topology control mechanism at lower layer, cross layer coding opportunity discover mechanism is presented to increase coding opportunities. In addition, a network coding aware energy efficient routing metric (NERM) is presented, which takes coding opportunity, node energy, and link quality into account jointly. Simulation results demonstrate that NAER improves the accuracy of coding discovery mechanism, increases the number of coding opportunities, saves node’s energy consumption, and prolongs network lifetime.

Energy efficiency is a critical problem for wireless sensor networks. In past decades, network coding has been proved as a promising technology to reduce the number of transmissions, save energy, and improve network throughput, especially suitable for the wireless network that motivates the emergence of network coding aware routings. However, existing network coding aware routings have the failure decoding problem, because of defective network coding conditions. On the other hand, existing network coding aware routings usually consider the ideal traffic scheduling and neglect nodes' energy and load which are important to wireless sensor networks. Therefore, it is not appropriate to directly apply existing network coding aware routings in wireless sensor networks. Regarding the problems above problems, this paper proposes traffic-shaped network coding aware routing (TSCAR) for wireless sensor networks. In the TSCAR, a universal network coding condition is presented to solve the false decoding problem. And traffic shaping mechanism is proposed to shape the traffic of different flows to create more coding actions when coding opportunities exist. In addition, network calculus is used to analyze the delay of the TSCAR. Besides, a coding, load, and energy aware routing metrics are proposed to evaluate the discovered paths. Extensive simulation results demonstrate that the TSCAR increases the number of coding opportunities and the proportion of coded packets, improves network throughput, and extends the network lifetime of wireless sensor networks.

Network coding–based routing has been widely considered as a promising technology to improve the network throughput of wireless multihop network. However, existing network coding aware routings have the problem of failure decoding and usually neglect the transmission scheduling, which degrades the gain of network coding. In this article, a common network coding condition and traffic matching supported network coding aware routing is proposed for wireless multihop network. In this, common network coding condition is proposed to avoid the problem of failed decoding. In addition, traffic matching mechanism is presented to promote the occurrence of network coding. Besides, a coding and load aware routing metric, which jointly considers the network coding opportunity and node load, is designed to evaluate the discovered paths. Extensive simulations on NS2 demonstrate that CTNR increases the number of coding opportunities and network coding occurrence, and improves network throughput.

To strengthen the prevention of cracks in hydration heat engineering. On the basis of summarizing and analyzing the models for hydration heat of concrete, numerical methods are used to calculate the temperature change curve of the core concrete in cement hydration stage. The thermal performance parameters of steel tubes and concrete are the essential basic data, which serves as a basis for research. Sectional dimension largely influences the shrinkage distortion of the core concrete in the concrete-filled steel tubes, which increases as the dimensions of the cross-section expand.

Copyright of Journal of Central South University is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Triphenyltin (TPT) is an organotin pollutant widely found in the aquatic environment. It has endocrine-disrupting and osmotic pressure toxicity. In this study, the physiological and biochemical effects of TPT and various salinities were investigated in different tissues (gut, gill, and brain) of marine medaka. The exposure experiments were conducted for 42 days in different salinities (0, 15, and 30 ppt) without TPT exposure and in different salinity groups with TPT exposure concentrations of 100 ng/L, respectively. The results showed that the Na+-K+-ATPase (NKA) and Ca2+ATPase activity had significant tissue-specific differences, with the highest activity observed in the gills, indicating their major contribution to osmoregulation. Changes in salinity also resulted in significant alterations in the ion concentrations of the gut and gill tissues in the 0-C and 15-C groups. While the changes in Na+ and Cl− were relatively stable, the presence of TPT disrupted the regulation of Ca2+ and K+. In conclusion, substantial variations were observed in the osmoregulatory capacity of marine medaka tissues. Environmental concentrations of TPT had little effect on osmotic enzyme activity but interfered with the regulation of Ca2+ and K+ concentrations in the tissues. This study provides valuable insights into the osmotic toxicity of TPT in aquatic environments with different salinities. [ABSTRACT FROM AUTHOR]

Aiming to address the problem that faults in rolling bearings make effective fault diagnosis difficult under small-sample and varying working conditions, this paper proposes a new fault diagnosis method for rolling bearings that monitors their vibration signals and is based on an improved deep residual Siamese neural network, called a WDRCNN. Firstly, the Siamese neural network is applied to extract features with shared weights to achieve an expansion in the number of fault samples. Then, multiple residual blocks are used to extract deeper feature information and effectively alleviate the problem of overfitting. In addition, the attention mechanism is employed to assign weights to the feature information to reduce the interference of redundant features. Finally, the Euclidean distance between the sample pairs is calculated to determine the similarity of the sample pairs and to classify bearing faults for end-to-end bearing fault diagnosis. The experimental results demonstrate that the WDRCNN achieves an average accuracy of 96.31% under different operating conditions. Even when only 90 training samples are available, the WDRCNN achieves an accuracy of over 93%. [ABSTRACT FROM AUTHOR]

Copyright of Irrigation & Drainage is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

As part of our continuous efforts to discover novel c-Met inhibitors as antitumor agents, four series of thiazole/thiadiazole carboxamide-derived analogues were designed, synthesised, and evaluated for the in vitro activity against c-Met and four human cancer cell lines. After five cycles of optimisation on structure–activity relationship, compound 51am was found to be the most promising inhibitor in both biochemical and cellular assays. Moreover, 51am exhibited potency against several c-Met mutants. Mechanistically, 51am not only induced cell cycle arrest and apoptosis in MKN-45 cells but also inhibited c-Met phosphorylation in the cell and cell-free systems. It also exhibited a good pharmacokinetic profile in BALB/c mice. Furthermore, the binding mode of 51am with both c-Met and VEGFR-2 provided novel insights for the discovery of selective c-Met inhibitors. Taken together, these results indicate that 51am could be an antitumor candidate meriting further development. [ABSTRACT FROM AUTHOR]

Klebsiella pneumoniae found in the normal flora of the human oral and intestinal tract mainly causes hospital-acquired infections but can also cause community-acquired infections. To date, most clinical trials of vaccines against K. pneumoniae have ended in failure. Furthermore, no single conserved protein has been identified as an antigen candidate to accelerate vaccine development. In this study, we identified five outer membrane proteins of K. pneumoniae , namely, Kpn_Omp001, Kpn_Omp002, Kpn_Omp003, Kpn_Omp004, and Kpn_Omp005, by using reliable second-generation proteomics and bioinformatics. Mice vaccinated with these five KOMPs elicited significantly higher antigen-specific IgG, IgG1, and IgG2a. However, only Kpn_Omp001, Kpn_Omp002, and Kpn_Omp005 were able to induce a protective immune response with two K. pneumoniae infection models. These protective effects were accompanied by the involvement of different immune responses induced by KOMPs, which included KOMPs-specific IFN-γ-, IL4-, and IL17A-mediated immune responses. These findings indicate that Kpn_Omp001, Kpn_Omp002, and Kpn_Omp005 are three potential Th1, Th2, and Th17 candidate antigens, which could be developed into multivalent and serotype-independent vaccines against K. pneumoniae infection. [ABSTRACT FROM AUTHOR]

In RFID (Radio Frequency IDentification) system, when multiple tags are in the operating range of one reader and send their information to the reader simultaneously, the signals of these tags are superimposed in the air, which results in a collision and leads to the degrading of tags identifying efficiency. To improve the multiple tags' identifying efficiency due to collision, a physical layer network coding based binary search tree algorithm (PNBA) is proposed in this paper. PNBA pushes the conflicting signal information of multiple tags into a stack, which is discarded by the traditional anti-collision algorithm. In addition, physical layer network coding is exploited by PNBA to obtain unread tag information through the decoding operation of physical layer network coding using the conflicting information in the stack. Therefore, PNBA reduces the number of interactions between reader and tags, and improves the tags identification efficiency. Theoretical analysis and simulation results using MATLAB demonstrate that PNBA reduces the number of readings, and improve RFID identification efficiency. Especially, when the number of tags to be identified is 100, the average needed reading number of PNBA is 83% lower than the basic binary search tree algorithm, 43% lower than reverse binary search tree algorithm, and its reading efficiency reaches 0.93. [ABSTRACT FROM AUTHOR]

Gypenosides have anticancer activity against many cancers. Gypenoside LI is a gypenoside monomer from Gynostemma pentaphyllum, its pharmacological functions in melanoma have not been reported. In this study, we found that gypenoside LI had a potent cytotoxic effect on melanoma cells. Gypenoside LI can induce intrinsic apoptosis along with S phase arrest. Furthermore, gypenoside LI inhibited the colony formation ability of melanoma through inhibition of the Wnt/β‐catenin signaling pathway. Interestingly, we also found that gypenoside LI can induce the upregulation of the tumor suppressor miR‐128‐3p during melanoma apoptosis. In contrast, gypenoside LI induced apoptosis, cell cycle arrest, and inhibition of the Wnt/β‐catenin signaling pathway, which were abolished by overexpression of the miR‐128‐3p inhibitor in A375 cells. Taken together, these results showed that gypenoside LI could inhibit human melanoma cells through inducing apoptosis, arresting cell cycle at the S phase and suppressing the Wnt/β‐catenin signaling pathway in a miR‐128‐3p dependent manner. [ABSTRACT FROM AUTHOR]

Three novel dammarane-type saponins, 2α,3β,12β,20(S),24(S)-pentahydroxydammar-25-ene-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-20-O-β-D-glucopyranoside (1, namely gypenoside J1), 2α,3β,12β,20(S),25-pentahydroxydammar-23-ene-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-20-O-β-D-glucopyranoside (2, namely gypenoside J2) and 2α,3β,12β,20(S)-tetrahydroxydammar-25-en-24-one-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-20-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside (3, namely gypenoside J3) along with one known gypenoside (gypenoside LVII) were isolated from the aerial parts of G. pentaphyllum using various chromatographic methods. Their structures were elucidated on the basis of IR, 1D- (1H and 13C), 2D-NMR spectroscopy (HSQC, HMBC and COSY), and mass spectrometry (ESI-MS/MS). Their activity was tested using CCK-8 assay. These four compounds showed little anti-cancer activity with IC50 values more than 100 μM against four types of human cancer lines. The effects of them against H2O2-induced oxidative stress in human neuroblastoma SH-SY5Y cells were evaluated and they all showed potential neuroprotective effects with 3.64–18.16% higher cell viability than the H2O2-induced model group. [ABSTRACT FROM AUTHOR]

Ethnopharmacological relevance Gypenosides are major constituents in Gynostemma pentaphyllum (Thunb.) Makino. Previous studies have shown that gypenosides isolated from G. pentaphyllum possess inhibitory effect on the growth of cancer cells, especially A549 cells, with structure-activity relationship (SAR). However, the underlying mechanism of gypenoside-induced A549 cell death remains to be clarified. Aim of the study To further investigate SAR and the underlying mechanism of gypenosides in A549 cells. Materials and methods Gypenosides were isolated from G. pentaphyllum using chromatography methods and identified using MS and NMR data. The cytotoxicity was determined with CCK-8 assay. The effects of gypenosides on apoptosis, cell cycle and migration were investigated through cell morphology observation, flow cytometry analysis and key proteins detection. Results Three gypenosides, 2 α ,3 β ,12 β ,20( S )-tetrahydroxydammar-24-ene-3-O- β -D-glucopyranoside-20-O- β -D-glucopyranoside, gypenoside L and gypenoside LI were isolated from G. pentaphyllum . Gypenoside stereoisomers, gypenoside L (S configuration at C20) and gypenoside LI (R configuration at C20) showed stronger activity against A549 cells. Furthermore, both induced A549 cell apoptosis through intrinsic and extrinsic pathways evidenced by reducing mitochondrial membrane potential (MMP), generating reactive oxygen species (ROS), releasing more cytochrome c and down-regulating procaspase 8. However, gypenoside L blocked A549 cells in G0/G1, while gypenoside LI induced G2/M arrest, which was further verified by different expression of CDK1, CDK2 and CDK4. In addition, both inhibited A549 cell migration, which was evidenced by down-regulation of MMP-2/9 as well as scratch wound assay and transwell assay. Conclusion C 20 of gypenoside played an important role in A549 cell cytotoxicity and gypenoside stereoisomers could be used as potential multi-target chemopreventive agents for cancer. [ABSTRACT FROM AUTHOR]

Gynostemma pentaphyllum, a traditional herbal tea, and its flavonoids possess antioxidant activities. This study was carried out to isolate and identify these flavonoids from G. pentaphyllum and determinate the contents of the main flavonoids and their antioxidant activities. Nine flavonoids were determinated rapidly from G. pentaphyllum using medium-pressure liquid chromatography combined with other simple chromatographic methods. And nine flavonoids were identified as rutin (1), 4′-O-methyl-kaempferol-3-O-rutinoside (2), ombuoside (3), kaempferol-3-β-D-O-rutinoside (4), isorhamnetin-3-O-β-D-rutinoside (5), quercetin-3-O-β-D-glucoside (6), isorhamnetin (7), kaempferol (8), and quercetin (9) by liquid chromatography mass spectrometry-ion trap time of flight and 13C nuclear magnetic resonance spectroscopy, respectively. All of them displayed various potent antioxidant effects against the DPPH radical and A549 cell injury by using H2O2-generate peroxyl radicals in vitro. A sensitive and specific ultra-performance liquid chromatography coupled with multiple-reaction monitoring mode was utilized to rapidly analyze the main six flavonoids of the ethanol extract of G. pentaphyllum within less than 8 min using a Waters Acquity UPLC BEH C18 column. The contents of them were from 57.11 to 12907.74 µg/g in G. pentaphyllum. [ABSTRACT FROM AUTHOR]

Control of surrounding rocks is one of the major problems in deep underground engineering under complicated conditions, such as high stress and tectonic fracture zones. This study proposed a three-dimensional confined concrete support system (CCSS) and investigated its main component: the confined concrete (CC) arch. The authors derived an internal force calculation formula for a circular arch with any number of sections and studied the effects of load, lateral pressure coefficient, node rigidity ratio, and other factors on the internal force distribution. In the analysis of CC part strength, damage first occurred to the CC159_10-C40 arch on both sides of the right and the left casings at an ultimate bearing capacity of 2,210 kN. To verify the correctness of the theoretical calculation and investigate the mechanical properties and damage mechanism of the arch, a full-scale indoor test on the CC159_10-C40 arch was conducted, and a 1:1 large-scale test system was developed. The authors found that the arch ultimate bearing capacity under the same load was 2,003.2 kN, a difference of only 9.36% compared with the theoretical calculation. The arch damage mechanism and key damaged parts were found to be consistent with the predictions of the theoretical calculations. The results of this research provide evidence for the design and analysis of CC arches. [ABSTRACT FROM AUTHOR]

The aim of the present study was to determine the function of human amniotic epithelial cell transplantation (hAECT) in promoting the healing of rats with stage III pressure ulcer (PU) and to initially investigate its possible mechanisms. A total of 96 Sprague Dawley rats were allocated at random into the model, hAECT, conventional treatment or control groups (n=24 per group). In each group, 6 rats were observed to determine the wound-healing rate. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF)-α in the wound tissue and serum were detected using reverse transcription-quantitative polymerase chain reaction analysis and enzyme-linked immunosorbent assay. The transplantation of hAECs significantly increased the healing rate of the stage III PUs and was accompanied by the significant upregulation of VEGF mRNA and protein expression levels and the significant downregulation of TNF-α mRNA and protein expression. Immunofluorescence staining showed that, on day 7 of transplantation, hAECs remained alive inside the skin tissues. Therefore, hAECT through subcutaneous injection appears to significantly improve the wound-healing rate of stage III PUs in rats, and this effect may be associated with the upregulation of the proangiogenic factor VEGF and the downregulation of the inflammatory cytokine TNF-α. [ABSTRACT FROM AUTHOR]

Aim: To explore the effect of androgen receptor (AR) on the expression of the cell cycle-related genes, such as CDKN1A and BTG1, in prostate cancer cell line LNCaP. Methods: After AR antagonist flutamide treatment and confirmation of its effect by phase contrast microscope and flow cytometry, the differential expression of the cell cycle-related genes was analyzed by a cDNA microarray. The flutamide treated cells were set as the experimental group and the LNCaP cells as the control. We labeled cDNA probes of the experimental group and control group with Cy5 and Cy3 dyes, respectively, through reverse transcription. Then we hybridized the cDNA probes with cDNA microarrays, which contained 8 126 unique human cDNA sequences and the chip was scanned to get the fluorescent values of Cy5 and Cy3 on each spot. After primary analysis, reverse transcription polymerase chain reaction (RT-PCR) tests were carried out to confirm the results of the chips. Results: After AR antagonist flutamide treatment, three hundred and twenty-six genes (3.93 %) expressed differentially, 97 down-regulated and 219 up-regulated. Among them, eight up-regulated genes might be cell cycle-related, namely CDC10, NRAS, BTG1, Wee1, CLK3, DKFZP564A122, CDKN1A and BTG2. The CDKN1A and BTG1 gene mRNA expression was confirmed to be higher in the experimental group by RT-PCR, while p53 mRNA expression had no significant changes. Conclusion: Flutamide treatment might up-regulate CDKN1A and BTG1 expression in prostate cancer cells. The protein expressions of CDKN1A and BTG1 play an important role in inhibiting the proliferation of cancer cells. CDKN1A has a great impact on the cell cycle of prostate cancer cells and may play a role in the cancer cells in a p53-independent pathway. The prostate cancer cells might affect the cell cycle-related genes by activating AR and thus break the cell cycle control. [ABSTRACT FROM AUTHOR]

Abstract H9N2 subtype influenza virus has become worldwide and prevalent in China. Previous studies illustrated that at least three sublineages had been established in terrestrial poultry of Eurasian avian. In this presentation, eight full-length genes of an H9N2 strain, A/Chicken/Shanghai/F/98 (Ck/SH/F/98) were obtained. Sequence analysis and phylogenetic studies were conducted by comparing eight genes with those of all the available H9N2 strains from the GenBank. The results showed that four genes (HA, NA, M and NS genes) of Ck/SH/F/98 were incorporated into the sublineage represented by the early mainland China strain, Ck/BJ/1/94. However, the other four of RNP genes of Ck/SH/F/98 did not show close relationship with those of the three known sublineages viruses. Therefore, Ck/SH/F/98 was a natural reassortant between different sublineages. In addition, comparison showed that Ck/SH/F/98 could be a putative precursor of a later isolate from southern China, Dk/ST/1605/01, with at least six genes of both closely related, indicating genes of Ck/SH/F/98 and early isolates had ever been circulating. Further comparison in terms of molecular markers of species specificity of HA1 revealed that DK/ST/1605/01 also resembled Ck/SH/F/98 more than a common earlier duck strain. The results supported the idea of two-way transmission between terrestrial and aquatic birds that emphasized the importance to raise concerns on the natural evolution of all the eight genes of H9N2 avian influenza viruses. [ABSTRACT FROM AUTHOR]