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4 results on '"Wolfgang Boenigk"'

1. The sperm-specific K+ channel Slo3 is inhibited by albumin and steroids contained in reproductive fluids

Author

Johannes Lorenz, Clara Eisenhardt, Teresa Mittermair, Alexandra E. Kulle, Paul Martin Holterhus, Manfred Fobker, Wolfgang Boenigk, Verena Nordhoff, Hermann M. Behre, Timo Strünker, and Christoph Brenker

Subjects
human sperm, ion channel, sperm signalling, reproductive tract, follicular fluid, Biology (General), QH301-705.5
Abstract

To locate and fertilize the egg, sperm probe the varying microenvironment prevailing at different stages during their journey across the female genital tract. To this end, they are equipped with a unique repertoire of mostly sperm-specific proteins. In particular, the flagellar Ca2+ channel CatSper has come into focus as a polymodal sensor used by human sperm to register ligands released into the female genital tract. Here, we provide the first comprehensive study on the pharmacology of the sperm-specific human Slo3 channel, shedding light on its modulation by reproductive fluids and their constituents. We show that seminal fluid and contained prostaglandins and Zn2+ do not affect the channel, whereas human Slo3 is inhibited in a non-genomic fashion by diverse steroids as well as by albumin, which are released into the oviduct along with the egg. This indicates that not only CatSper but also Slo3 harbours promiscuous ligand-binding sites that can accommodate structurally diverse molecules, suggesting that Slo3 is involved in chemosensory signalling in human sperm.

Published
2024
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2. Antiviral signaling by a cyclic nucleotide activated CRISPR protease

Author

Christophe Rouillon, Niels Schneberger, Haotian Chi, Katja Blumenstock, Stefano Da Vela, Katrin Ackermann, Jonas Moecking, Martin F. Peter, Wolfgang Boenigk, Reinhard Seifert, Bela E. Bode, Jonathan L. Schmid-Burgk, Dmitri Svergun, Matthias Geyer, Malcolm F. White, Gregor Hagelueken, European Research Council, University of St Andrews. School of Biology, University of St Andrews. School of Chemistry, University of St Andrews. Institute of Behavioural and Neural Sciences, University of St Andrews. EaSTCHEM, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. Centre of Magnetic Resonance, and University of St Andrews. St Andrews Bioinformatics Unit

Subjects
MCC, QR355, QH301, Multidisciplinary, QH301 Biology, DAS, QR355 Virology
Abstract

Funding information: M.G. and J.L.S.B. are funded by the Deutsche Forschungsgemeinschaft under Germany’s Excellence Strategy–EXC2151–390873048. M.F.W. acknowledges a European Research Council Advanced Grant (grant number 101018608) and the China Scholarship Council (REF: 202008420207 to H.C.). G.H. is grateful for funding by the Deutsche Forschungsgemeinschaft (grant number HA6805/6-1). CRISPR defense systems such as the well-known DNA-targeting Cas9 and the RNA-targeting type III systems are widespread in prokaryotes1,2. The latter can orchestrate a complex antiviral response that is initiated by the synthesis of cyclic oligoadenylates (cOAs) upon foreign RNA recognition3-5. Among a large set of proteins that were linked to type III systems and predicted to bind cOAs6,7, a CRISPR associated Lon protease (CalpL) stood out to us. The protein contains a sensor domain of the SAVED (SMODS-associated and fused to various effector domains) family7, fused to a Lon protease effector domain. However, the mode of action of this effector was unknown. Here, we report the structure and function of CalpL and show that the soluble protein forms a stable tripartite complex with two further proteins, CalpT and CalpS, that are encoded in the same operon. Upon activation by cA4, CalpL oligomerizes and specifically cleaves the MazF-homolog CalpT, releasing the extracytoplasmic function (ECF) sigma factor CalpS from the complex. This provides a direct connection between CRISPR-based foreign nucleic acid detection and transcriptional regulation. Furthermore, the presence of a cA4-binding SAVED domain in a CRISPR effector reveals an unexpected link to the cyclic oligonucleotide-based antiphage signaling system (CBASS). Postprint

Published
2023

3. Klimaschwankungen im Frühweichsel der Lößabfolgen des Mittelrheingebiets

Author

Wolfgang Boenigk and Manfred Frechen

Subjects
551.7, lumineszens dating, lcsh:Geology, middle rhine area, Glazialgeologie, tönchesberg, lcsh:QE1-996.5, palaeosol, VAR 000, germany, loess, greenland ice cores
Abstract

Am Tönchesberg und in Koblenz-Metternich sind über dem Bt-Horizont des Eems (OIS 5e) bis an die Basis des Oberweichsel-Löß (OIS 2) zehn eigenständige Bodenbildungen nachgewiesen, die durch Sedimentationsphasen voneinander getrennt sind. Bis auf die beiden jüngsten, die als braune Tundrenböden anzusprechen sind, handelt es sich um gut ausgeprägte Ah-Horizonte, z. T. um Bv-, Bt-Ah- bzw. Bt-Horizonte., research

Published
2018

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