11 results on '"Weers B"'
Search Results
2. JGI Plant Gene Atlas: an updateable transcriptome resource to improve functional gene descriptions across the plant kingdom.
- Author
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Sreedasyam A, Plott C, Hossain MS, Lovell JT, Grimwood J, Jenkins JW, Daum C, Barry K, Carlson J, Shu S, Phillips J, Amirebrahimi M, Zane M, Wang M, Goodstein D, Haas FB, Hiss M, Perroud PF, Jawdy SS, Yang Y, Hu R, Johnson J, Kropat J, Gallaher SD, Lipzen A, Shakirov EV, Weng X, Torres-Jerez I, Weers B, Conde D, Pappas MR, Liu L, Muchlinski A, Jiang H, Shyu C, Huang P, Sebastian J, Laiben C, Medlin A, Carey S, Carrell AA, Chen JG, Perales M, Swaminathan K, Allona I, Grattapaglia D, Cooper EA, Tholl D, Vogel JP, Weston DJ, Yang X, Brutnell TP, Kellogg EA, Baxter I, Udvardi M, Tang Y, Mockler TC, Juenger TE, Mullet J, Rensing SA, Tuskan GA, Merchant SS, Stacey G, and Schmutz J
- Subjects
- Gene Expression Regulation, Plant, Genome, Plant, Phylogeny, Software, Atlases as Topic, Genes, Plant, Transcriptome genetics
- Abstract
Gene functional descriptions offer a crucial line of evidence for candidate genes underlying trait variation. Conversely, plant responses to environmental cues represent important resources to decipher gene function and subsequently provide molecular targets for plant improvement through gene editing. However, biological roles of large proportions of genes across the plant phylogeny are poorly annotated. Here we describe the Joint Genome Institute (JGI) Plant Gene Atlas, an updateable data resource consisting of transcript abundance assays spanning 18 diverse species. To integrate across these diverse genotypes, we analyzed expression profiles, built gene clusters that exhibited tissue/condition specific expression, and tested for transcriptional response to environmental queues. We discovered extensive phylogenetically constrained and condition-specific expression profiles for genes without any previously documented functional annotation. Such conserved expression patterns and tightly co-expressed gene clusters let us assign expression derived additional biological information to 64 495 genes with otherwise unknown functions. The ever-expanding Gene Atlas resource is available at JGI Plant Gene Atlas (https://plantgeneatlas.jgi.doe.gov) and Phytozome (https://phytozome.jgi.doe.gov/), providing bulk access to data and user-specified queries of gene sets. Combined, these web interfaces let users access differentially expressed genes, track orthologs across the Gene Atlas plants, graphically represent co-expressed genes, and visualize gene ontology and pathway enrichments., (© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2023
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3. Bioenergy sorghum stem growth regulation: intercalary meristem localization, development, and gene regulatory network analysis.
- Author
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Yu KMJ, Oliver J, McKinley B, Weers B, Fabich HT, Evetts N, Conradi MS, Altobelli SA, Marshall-Colon A, and Mullet J
- Subjects
- Gene Regulatory Networks, Gene Expression Regulation, Plant genetics, Brassinosteroids, Indoleacetic Acids metabolism, Cytokinins, Edible Grain metabolism, Hormones, Sorghum metabolism
- Abstract
Bioenergy sorghum is a highly productive drought tolerant C
4 grass that accumulates 80% of its harvestable biomass in approximately 4 m length stems. Stem internode growth is regulated by development, shading, and hormones that modulate cell proliferation in intercalary meristems (IMs). In this study, sorghum stem IMs were localized above the pulvinus at the base of elongating internodes using magnetic resonance imaging, microscopy, and transcriptome analysis. A change in cell morphology/organization occurred at the junction between the pulvinus and internode where LATERAL ORGAN BOUNDARIES (SbLOB), a boundary layer gene, was expressed. Inactivation of an AGCVIII kinase in DDYM (dw2) resulted in decreased SbLOB expression, disrupted IM localization, and reduced internode cell proliferation. Transcriptome analysis identified approximately 1000 genes involved in cell proliferation, hormone signaling, and other functions selectively upregulated in the IM compared with a non-meristematic stem tissue. This cohort of genes is expressed in apical dome stem tissues before localization of the IM at the base of elongating internodes. Gene regulatory network analysis identified connections between genes involved in hormone signaling and cell proliferation. The results indicate that gibberellic acid induces accumulation of growth regulatory factors (GRFs) known to interact with ANGUSTIFOLIA (SbAN3), a master regulator of cell proliferation. GRF:AN3 was predicted to induce SbARF3/ETT expression and regulate SbAN3 expression in an auxin-dependent manner. GRFs and ARFs regulate genes involved in cytokinin and brassinosteroid signaling and cell proliferation. The results provide a molecular framework for understanding how hormone signaling regulates the expression of genes involved in cell proliferation in the stem IM., (© 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)- Published
- 2022
- Full Text
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4. zmm28 transgenic maize increases both N uptake- and N utilization-efficiencies.
- Author
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Fernandez JA, Habben JE, Schussler JR, Masek T, Weers B, Bing J, and Ciampitti IA
- Subjects
- Plant Leaves genetics, Plant Leaves metabolism, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, Nitrogen metabolism, Zea mays genetics, Zea mays metabolism
- Abstract
Biotechnology has emerged as a valuable tool in the development of maize (Zea mays L.) hybrids with enhanced nitrogen (N) use efficiency. Recent work has described the positive effects of an increased and extended expression of the zmm28 transcription factor (Event DP202216) on maize yield productivity. In this study, we expand on the previous findings studying maize N uptake and utilization in DP202216 transgenic hybrids compared to wild-type (WT) controls. Isotope
15 N labeling demonstrates that DP202216 hybrids have an improved N uptake during late-vegetative stages (inducing N storage in lower leaves of the canopy) and, thus, N uptake efficiency (N uptake to applied N ratio) relative to WT. Through both greater N harvest index and reproductive N remobilization, transgenic plants were able to achieve better N utilization efficiency (yield to N uptake ratio). Our findings suggest the DP202216 trait could open new avenues for improving N uptake and utilization efficiencies in maize., (© 2022. The Author(s).)- Published
- 2022
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5. A vegetative storage protein improves drought tolerance in maize.
- Author
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Abbaraju HKR, Gupta R, Appenzeller LM, Fallis LP, Hazebroek J, Zhu G, Bourett TM, Howard RJ, Weers B, Lafitte RH, Hakimi SM, Schussler JR, Loussaert DF, Habben JE, and Dhugga KS
- Subjects
- Chloroplasts, Edible Grain genetics, Nitrogen metabolism, Plant Proteins genetics, Plant Proteins metabolism, Plants, Genetically Modified genetics, Droughts, Zea mays genetics
- Abstract
Vegetative storage proteins (VSPs) are known to serve as nitrogen reserves in many dicot plants but remain undiscovered in grasses, most widely grown group of crops globally. We identified and characterized a VSP in maize and demonstrated that its overexpression improved drought tolerance. Nitrogen supplementation selectively induced a mesophyll lipoxygenase (ZmLOX6), which was targeted to chloroplasts by a novel N-terminal transit peptide of 62 amino acids. When ectopically expressed under the control of various tissue-specific promoters, it accumulated to a fivefold higher level upon expression in the mesophyll cells than the wild-type plants. Constitutive expression or targeted expression specifically to the bundle sheath cells increased its accumulation by less than twofold. The overexpressed ZmLOX6 was remobilized from the leaves like other major proteins during grain development. Evaluated in the field over locations and years, transgenic hybrids overexpressing ZmLOX6 in the mesophyll cells significantly outyielded nontransgenic sibs under managed drought stress imposed at flowering. Additional storage of nitrogen as a VSP in maize leaves ameliorated the effect of drought on grain yield., (© 2021 Pioneer Hi-Bred International, Inc (Corteva Agriscience). Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
- Published
- 2022
- Full Text
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6. Low-field magnetic resonance imaging of roots in intact clayey and silty soils.
- Author
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Bagnall GC, Koonjoo N, Altobelli SA, Conradi MS, Fukushima E, Kuethe DO, Mullet JE, Neely H, Rooney WL, Stupic KF, Weers B, Zhu B, Rosen MS, and Morgan CLS
- Abstract
The development of a robust method to non-invasively visualize root morphology in natural soils has been hampered by the opaque, physical, and structural properties of soils. In this work we describe a novel technology, low field magnetic resonance imaging (LF-MRI), for imaging energy sorghum ( Sorghum bicolor (L.) Moench) root morphology and architecture in intact soils. The use of magnetic fields much weaker than those used with traditional MRI experiments reduces the distortion due to magnetic material naturally present in agricultural soils. A laboratory based LF-MRI operating at 47 mT magnetic field strength was evaluated using two sets of soil cores: 1) soil/root cores of Weswood silt loam (Udifluventic Haplustept) and a Belk clay (Entic Hapluderts) from a conventionally tilled field, and 2) soil/root cores from rhizotrons filled with either a Houston Black (Udic Haplusterts) clay or a sandy loam purchased from a turf company. The maximum soil water nuclear magnetic resonance (NMR) relaxation time T
2 (4 ms) and the typical root water relaxation time T2 (100 ms) are far enough apart to provide a unique contrast mechanism such that the soil water signal has decayed to the point of no longer being detectable during the data collection time period. 2-D MRI projection images were produced of roots with a diameter range of 1.5-2.0 mm using an image acquisition time of 15 min with a pixel resolution of 1.74 mm in four soil types. Additionally, we demonstrate the use of a data-driven machine learning reconstruction approach, Automated Transform by Manifold Approximation (AUTOMAP) to reconstruct raw data and improve the quality of the final images. The application of AUTOMAP showed a SNR (Signal to Noise Ratio) improvement of two fold on average. The use of low field MRI presented here demonstrates the possibility of applying low field MRI through intact soils to root phenotyping and agronomy to aid in understanding of root morphology and the spatial arrangement of roots in situ .- Published
- 2020
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7. Identification and characterization of a novel stay-green QTL that increases yield in maize.
- Author
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Zhang J, Fengler KA, Van Hemert JL, Gupta R, Mongar N, Sun J, Allen WB, Wang Y, Weers B, Mo H, Lafitte R, Hou Z, Bryant A, Ibraheem F, Arp J, Swaminathan K, Moose SP, Li B, and Shen B
- Subjects
- Biomass, Edible Grain genetics, Edible Grain growth & development, Nitrogen, Plant Leaves, Plant Proteins genetics, Plants, Genetically Modified, Zea mays growth & development, Photosynthesis, Quantitative Trait Loci, Transcription Factors genetics, Zea mays genetics
- Abstract
Functional stay-green is a valuable trait that extends the photosynthetic period, increases source capacity and biomass and ultimately translates to higher grain yield. Selection for higher yields has increased stay-green in modern maize hybrids. Here, we report a novel QTL controlling functional stay-green that was discovered in a mapping population derived from the Illinois High Protein 1 (IHP1) and Illinois Low Protein 1 (ILP1) lines, which show very different rates of leaf senescence. This QTL was mapped to a single gene containing a NAC-domain transcription factor that we named nac7. Transgenic maize lines where nac7 was down-regulated by RNAi showed delayed senescence and increased both biomass and nitrogen accumulation in vegetative tissues, demonstrating NAC7 functions as a negative regulator of the stay-green trait. More importantly, crosses between nac7 RNAi parents and two different elite inbred testers produced hybrids with prolonged stay-green and increased grain yield by an average 0.29 megagram/hectare (4.6 bushel/acre), in 2 years of multi-environment field trials. Subsequent RNAseq experiments, one employing nac7 RNAi leaves and the other using leaf protoplasts overexpressing Nac7, revealed an important role for NAC7 in regulating genes in photosynthesis, chlorophyll degradation and protein turnover pathways that each contribute to the functional stay-green phenotype. We further determined the putative target of NAC7 and provided a logical extension for the role of NAC7 in regulating resource allocation from vegetative source to reproductive sink tissues. Collectively, our findings make a compelling case for NAC7 as a target for improving functional stay-green and yields in maize and other crops., (© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
- Published
- 2019
- Full Text
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8. Overexpression of zmm28 increases maize grain yield in the field.
- Author
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Wu J, Lawit SJ, Weers B, Sun J, Mongar N, Van Hemert J, Melo R, Meng X, Rupe M, Clapp J, Haug Collet K, Trecker L, Roesler K, Peddicord L, Thomas J, Hunt J, Zhou W, Hou Z, Wimmer M, Jantes J, Mo H, Liu L, Wang Y, Walker C, Danilevskaya O, Lafitte RH, Schussler JR, Shen B, and Habben JE
- Subjects
- Amino Acid Sequence, Crops, Agricultural enzymology, Glutamate-Ammonia Ligase metabolism, Nitrate Reductase metabolism, Nitrogen metabolism, Photosynthesis genetics, Plant Leaves physiology, Plant Proteins chemistry, Plant Proteins genetics, Plant Proteins metabolism, Plants, Genetically Modified, Protein Binding, Transcriptome, Zea mays enzymology, Crops, Agricultural genetics, Edible Grain, Genes, Plant, Zea mays genetics
- Abstract
Increasing maize grain yield has been a major focus of both plant breeding and genetic engineering to meet the global demand for food, feed, and industrial uses. We report that increasing and extending expression of a maize MADS-box transcription factor gene, zmm28 , under the control of a moderate-constitutive maize promoter, results in maize plants with increased plant growth, photosynthesis capacity, and nitrogen utilization. Molecular and biochemical characterization of zmm28 transgenic plants demonstrated that their enhanced agronomic traits are associated with elevated plant carbon assimilation, nitrogen utilization, and plant growth. Overall, these positive attributes are associated with a significant increase in grain yield relative to wild-type controls that is consistent across years, environments, and elite germplasm backgrounds., Competing Interests: Competing interest statement: This work was funded by Corteva Agriscience, a for-profit agricultural technology company, as part of its research and development program., (Copyright © 2019 the Author(s). Published by PNAS.)
- Published
- 2019
- Full Text
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9. Extensive variation in the density and distribution of DNA polymorphism in sorghum genomes.
- Author
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Evans J, McCormick RF, Morishige D, Olson SN, Weers B, Hilley J, Klein P, Rooney W, and Mullet J
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- Chromosome Mapping, Chromosomes, Plant, DNA Methylation, Euchromatin genetics, Evolution, Molecular, Genotype, Haplotypes, Phylogeny, Polymorphism, Single Nucleotide, Recombination, Genetic, Selection, Genetic, Sequence Analysis, DNA, Genome, Plant, Polymorphism, Genetic, Sorghum genetics
- Abstract
Sorghum genotypes currently used for grain production in the United States were developed from African landraces that were imported starting in the mid-to-late 19(th) century. Farmers and plant breeders selected genotypes for grain production with reduced plant height, early flowering, increased grain yield, adaptation to drought, and improved resistance to lodging, diseases and pests. DNA polymorphisms that distinguish three historically important grain sorghum genotypes, BTx623, BTx642 and Tx7000, were characterized by genome sequencing, genotyping by sequencing, genetic mapping, and pedigree-based haplotype analysis. The distribution and density of DNA polymorphisms in the sequenced genomes varied widely, in part because the lines were derived through breeding and selection from diverse Kafir, Durra, and Caudatum race accessions. Genomic DNA spanning dw1 (SBI-09) and dw3 (SBI-07) had identical haplotypes due to selection for reduced height. Lower SNP density in genes located in pericentromeric regions compared with genes located in euchromatic regions is consistent with background selection in these regions of low recombination. SNP density was higher in euchromatic DNA and varied >100-fold in contiguous intervals that spanned up to 300 Kbp. The localized variation in DNA polymorphism density occurred throughout euchromatic regions where recombination is elevated, however, polymorphism density was not correlated with gene density or DNA methylation. Overall, sorghum chromosomes contain distal euchromatic regions characterized by extensive, localized variation in DNA polymorphism density, and large pericentromeric regions of low gene density, diversity, and recombination.
- Published
- 2013
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10. Regulation of pyrimidine metabolism in plants.
- Author
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Kafer C, Zhou L, Santoso D, Guirgis A, Weers B, Park S, and Thornburg R
- Subjects
- Models, Chemical, Plants enzymology, Pyrimidine Nucleotides biosynthesis, Pyrimidine Nucleotides chemistry, Pyrimidines biosynthesis, Pyrimidines chemistry, Plants metabolism, Pyrimidine Nucleotides metabolism, Pyrimidines metabolism
- Abstract
Pyrimidine nucleotides represent one of the most fundamental of cellular components. They are the building blocks for the direct synthesis of DNA and RNA that function in information storage and retrieval within the cell, but they also participate in the metabolism of a large number of other cellular components from sugar interconversion to cellular polysaccharides to glycoproteins and phospholipids. Thus, the metabolism of pyrimidine nucleotides and their intracellular pool sizes influence vast areas of normal cellular metabolism. The first pyrimidine, UMP, is synthesized by a de novo pathway that appears to be mechanistically invariant in all organisms. UMP is then further modified to form other pyrimidines. Breakdown of deoxyribo- and ribonucleic acids, the main sink for pyrimidine nucleotides, allows pyrimidines to be reutilized for resynthesis of these important cellular components. Pyrimidines are salvaged by converting the modified components into the free base, uracil for reutilization. Finally, pyrimidines are degraded into simple cellular metabolites permitting reutilization of nitrogen and carbon from pyrimidine ring systems into cellular metabolic pools. The regulation of pyrimidine metabolism is tightly controlled in plants. Additionally, plants produce toxic secondary metabolites derived from pyrimidines for use as defense compounds.
- Published
- 2004
- Full Text
- View/download PDF
11. DNA persistence length revisited.
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Lu Y, Weers B, and Stellwagen NC
- Subjects
- Biopolymers chemistry, Biopolymers isolation & purification, Birefringence, DNA isolation & purification, DNA Restriction Enzymes, Magnesium, Plasmids chemistry, Plasmids isolation & purification, Sodium, Temperature, DNA chemistry
- Abstract
DNA restriction fragments ranging from 79 to 789 base pairs in length have been characterized by transient electric birefringence (TEB) measurements at various temperatures between 4 and 43 degrees C. The DNA fragments do not contain runs of four or more adenine residues in a row and migrate with normal electrophoretic mobilities in polyacrylamide gels, indicating that they are not intrinsically curved or bent. The low ionic strength buffers used for the measurements contained 1 mM Tris Cl, pH 8.0, EDTA, and variable concentrations of Na(+) or Mg(2+) ions. The rotational relaxation times were obtained by fitting the TEB field-free decay signals with a nonlinear least-squared fitting program; the decay of the birefringence was monoexponential for fragments < or = 241 base pair (bp) in length and multiexponential for larger fragments. The terminal relaxation times, characteristic of the end-over-end rotation of the DNA molecules, were then used to determine the persistence length (p) and hydrodynamic radius (r) of DNA as a function of temperature and ionic strength, using several different hydrodynamic models. The specific values obtained for p and r are model dependent. The wormlike chain model of P. J. Hagerman and B. H. Zimm (Biopolymers 1981, Vol. 20, pp. 1481-1502) combined with the revised Broersma equation (J. Newman et al., Journal of Mol Biol 1997, Vol. 116, pp. 593-606) appears to be the most suitable for describing the flexibility of DNA in low ionic strength solutions. The values of p and r obtained from the global least squares fitting of this equation are independent of DNA length, and the deviations of the individual values from the average are reasonably small. The consensus r value calculated for DNA in various low ionic strength solutions containing 1 mM Tris buffer is 14.7 +/- 0.4 A at 20 degrees C. The consensus p values decrease from 814 approximately 564 A in solutions containing 1 mM Tris buffer plus 0.2-1 mM NaCl and decrease still further to 440 A in solutions containing 0.2 mM Mg(2+) ions. The persistence length exhibits a shallow maximum at 20 degrees C and decreases slowly upon either increasing or decreasing the temperature, regardless of the model used to fit the data. By contrast, the consensus values of the hydrodynamic radius are independent of temperature. The calculated persistence lengths and hydrodynamic radii are compared with other data in the literature., (Copyright 2002 Wiley Periodicals, Inc.)
- Published
- 2001
- Full Text
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