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964 results on '"Warnecke, G"'

10. Outcomes of Ischemic Papillary Muscle Rupture—A Single Center Experience.

Author

Dib, B., Gussinklo, W. te, Arif, R., Warnecke, G., Karck, M., and Sommer, W.

Subjects
MUSCLE injuries, PAPILLARY muscles, MITRAL valve surgery, MYOCARDIAL infarction, SURGICAL emergencies
Abstract

This article discusses the outcomes of emergency surgery for acute ischemic papillary muscle rupture, a rare complication following a heart attack. The study analyzed a cohort of 51 patients who underwent surgery for this condition between 2001 and 2023. The majority of patients presented with acute or subacute myocardial infarction prior to surgery, and a significant number required mechanical ventilation or CPR. The in-hospital mortality rate was high at 27.5%, but long-term survival following hospital discharge was excellent, with a 1-year survival rate of 71.6%. [Extracted from the article]

Published
2024
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13. Reconstruction of the miR-506-Quaking axis in Idiopathic Pulmonary Fibrosis using integrative multi-source bioinformatics

Author

Stojanovic, S.D., Fuchs, M., Liang, C., Schmidt, K., Xiao, K., Just, A., Pfanne, A., Pich, A., Warnecke, G., Braubach, P., Petzold, C., Jonigk, D., Distler, J.H.W., Fiedler, J., Thum, T., Kunz, M., and Publica

Subjects
Science, Primary Cell Culture, Datasets as Topic, Diseases, Pathogenesis, Article, Protein Interaction Mapping, Humans, Gene Regulatory Networks, ddc:610, Protein Interaction Maps, Lung, Cells, Cultured, Gene Expression Profiling, Computational Biology, RNA-Binding Proteins, Fibroblasts, respiratory system, Idiopathic Pulmonary Fibrosis, Computational biology and bioinformatics, respiratory tract diseases, MicroRNAs, Gene Expression Regulation, Case-Control Studies, Medicine, Single-Cell Analysis
Abstract

The family of RNA-binding proteins (RBP) functions as a crucial regulator of multiple biological processes and diseases. However, RBP function in the clinical setting of idiopathic pulmonary fibrosis (IPF) is still unknown. We developed a practical in silico screening approach for the characterization of RBPs using multi-sources data information and comparative molecular network bioinformatics followed by wet-lab validation studies. Data mining of bulk RNA-Sequencing data of tissues of patients with IPF identified Quaking (QKI) as a significant downregulated RBP. Cell-type specific expression was confirmed by single-cell RNA-Sequencing analysis of IPF patient data. We systematically analyzed the molecular interaction network around QKI and its functional interplay with microRNAs (miRs) in human lung fibroblasts and discovered a novel regulatory miR-506-QKI axis contributing to the pathogenesis of IPF. The in silico results were validated by in-house experiments applying model systems of miR and lung biology. This study supports an understanding of the intrinsic molecular mechanisms of IPF regulated by the miR-506-QKI axis. Initially applied to human lung disease, the herein presented integrative in silico data mining approach can be adapted to other disease entities, underlining its practical relevance in RBP research.

Published
2021

46. Clinical Molecular Imaging of Pulmonary CXCR4 Expression to Predict Outcome of Pirfenidone Treatment in Idiopathic Pulmonary Fibrosis

Author

Derlin, T., Jaeger, B., Jonigk, D., Apel, R.M., Freise, J., Shin, H.-O., Weiberg, D., Warnecke, G., Ross, T.L., Wester, H.-J., Seeliger, B., Welte, T., Bengel, F.M., Prasse, A., and Publica

Abstract

Background. Idiopathic pulmonary fibrosis (IPF) is a progressive disease for which two antifibrotic drugs recently were approved. However, an unmet need exists to predict responses to antifibrotic treatment, such as pirfenidone. Recent data suggest that upregulated expression of CXCR4 is indicative of outcomes in IPF. Research Question. Can quantitative, molecular imaging of pulmonary CXCR4 expression as a biomarker for disease activity predict response to the targeted treatment pirfenidone and prognosis in patients with IPF? Study Design and Methods. CXCR4 expression was analyzed by immunohistochemistry examination of lung tissues and reverse-transcriptase polymerase chain reaction analysis of BAL. PET-CT scanning with the specific CXCR4 ligand 68Ga-pentixafor was performed in 28 IPF patients and compared with baseline clinical characteristics. In 16 patients, a follow-up scan was obtained 6 to 12 weeks after initiation of treatment with pirfenidone. Patients were followed up in our outpatient clinic for > 12 month. Results. Immunohistochemistry analysis showed high CXCR4 staining of epithelial cells and macrophages in areas with vast fibrotic remodeling. Targeted PET scanning revealed CXCR4 upregulation in fibrotic areas of the lungs, particularly in zones with subpleural honeycombing. Baseline CXCR4 signal demonstrated a significant correlation with Gender Age Physiology stage (r = 0.44; P = .02) and with high-resolution CT scan score (r = 0.38; P = .04). Early changes in CXCR4 signal after initiation of pirfenidone treatment correlated with the long-term course of FVC after 12 months (r = −0.75; P = .0008). Moreover, patients with a high pulmonary CXCR4 signal on follow-up PET scan after 6 weeks into treatment demonstrated a statistically significant worse outcome at 12 months (P = .002). In multiple regression analysis, pulmonary CXCR4 signal on follow-up PET scan emerged as the only independent predictor of long-term outcome (P = .0226). Interpretation. CXCR4-targeted PET imaging identified disease activity and predicted outcome of IPF patients treated with pirfenidone. It may serve as a future biomarker for personalized guidance of antifibrotic treatment.

Published
2021

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