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There is a heated debate over the necessity of nitrogen (N) reduction, in addition to phosphorus (P) reduction, for the control of eutrophication. Whole-lake fertilization experiments and lake restoration practices in high latitudes have demonstrated that P is the primary factor regulating total phytoplankton. Recognizing the limited large-scale evidence in warmer climatic zones, a fertilization experiment was conducted in four ponds located in the subtropical Yangtze River Basin, China. Total phytoplankton abundance in a pond receiving P (+P) was similar to that in a pond receiving both N and P (+N+P). Both had higher phytoplankton than a pond receiving no additional nutrient (Control). Total nitrogen concentration (TN) in the +P pond increased with the appearance of N-fixing cyanobacteria. Total phytoplankton abundance was similar in the ponds without P addition (+N, Control), and both ponds had lower phytoplankton levels than the +N+P pond. These results showed that P, not N, determines total phytoplankton abundance and that N deficiency is offset by N fixation in subtropical lakes. This experiment supports the idea that attention should be mainly focused on P reduction in mitigating eutrophication. Un debat existe concernant la necessite de reduire l'azote (N), en plus du phosphore (P), pour controler l'eutrophisation. Des experiences de fertilisation a l'echelle des lacs et les pratiques de restauration des lacs a hautes latitudes ont demontre que le P est le principal facteur de regulation du phytoplancton total. Reconnaissant le caractere limite des donnees a grande echelle pour les zones climatiques plus chaudes, nous avons mene une experience de fertilisation dans quatre etangs situes dans le bassin subtropical du fleuve Yangzi (Chine). L'abondance du phytoplancton total dans un etang recevant du P (+P) etait semblable a celle d'un etang recevant du N et du P (+N+P). Les deux avaient plus de phytoplancton qu'un etang ne recevant aucun ajout de nutriment (temoin). La concentration d'azote total (TN) dans l'etang +P augmentait avec l'apparition de cyanobacteries fixatrices de N. L'abondance du phytoplancton total etait semblable pour les etangs sans ajout de P (+N, temoin), et l'abondance de phytoplancton dans ces deux etangs etait plus faible que dans l'etang +N+P. Ces resultats montrent que le P, et non le N, determine l'abondance de phytoplancton total et que la fixation de N compense les deficiences en N dans les lacs subtropicaux. Cette experience appuie la notion voulant que les efforts d'attenuation de l'eutrophisation devraient principalement porter sur la reduction du P. [Traduit par la Redaction], Introduction Eutrophication of lakes is a global environmental problem, associated with algal blooms, fish kills, ecosystem degeneration, and toxic algae, and hence represents a hazard to drinking water supplies. Many [...]

Due to excess nutrient loading, loss of submersed macrophytes is a worldwide phenomenon in shallow lakes. Phosphorus is known to contribute significantly to macrophyte recession, but the role of nitrogen has received increasing attention. Our understanding of how high nitrogen concentrations affect the growth of submersed macrophytes, particularly under natural conditions, is still limited. In this study, we conducted experiments with canopy-forming Potamogeton crispus in 10 ponds subjected to substantial differences in nitrogen loading (five targeted total nitrogen concentrations: control, 2, 10, 20, and 100 mg L-1) and compared the results with those of our earlier published experiments with rosette-forming Vallisneria natans performed 1 year before. Canopy-forming P. crispus was more tolerant than rosette-forming V. natans to exposure to high NH4 concentrations. This is probably because canopy-forming species reach the water surface where there is sufficient light for production of carbohydrates, thereby allowing the plants to partly overcome high NH4 stress. Both the canopy-forming P. crispus and the rosette-forming V. natans showed clear declining trends with increasing chlorophyll a in the water. Accordingly, shading by phytoplankton might be of key importance for the decline in submersed macrophytes in this experiment. Both experiments revealed free amino acids (FAA) to be a useful indicator of physiological stress by high ammonium but is not a reliable indicator of macrophyte growth. [ABSTRACT FROM AUTHOR]

Published research suggests that the total phosphorus ( TP) thresholds for the regime shifts between a clear-water state dominated by submersed macrophytes and a turbid-water state dominated by phytoplankton in shallow lakes vary with forms of lake basins and climates. However, such hypotheses remain untested by direct field evidence. We therefore tested the hypotheses with empirical data from subtropical lakes on the Yangtze floodplain and also from other lakes in temperate to tropical zones., TP thresholds were found to vary little at moderate depths, but to decrease notably when depth exceeds a level of probably 3-4 m, and increase sharply when depth is below a level of around 1-2 m., TP thresholds were found to be nearly equal in shallow lakes (1-2 m

Urban lakes are important natural assets but are exposed to multiple stressors from human activities. Submersed macrophytes, a key plant group that helps to maintain clear-water conditions in lakes, tend to be scarce in urban lakes, particularly when they are eutrophic or hypertrophic, and their loss is linked, in part, to impaired underwater light climate. We tested if enhancing the underwater light conditions using light-emitting diodes (LEDs) could restore submersed macrophytes in urban lakes. Twelve mesocosms (1000 L each) were each planted with tape grass (Vallisneria natans) and monitored over three months (22 August–7 November), using a control and three artificial light intensity treatments (10, 50, and 100 µmol m-2 s-1). Compared with the control, the high light treatment (100 µmol m-2 s-1) had higher leaf number, maximum leaf length, and average leaf length (3.9, 5.8, and 2.8 times, respectively). Shoot number, leaf number, leaf dry mass, root dry mass, and photosynthetic photon flux density in the high-light treatment were significantly greater than the control, but root length and phytoplankton chlorophyll a were not related to plant growth variables and were low in all treatments. Periphyton chlorophyll a increased significantly with the plant growth variables (i.e., shoot number, leaf number, and maximum leaf length) and was high in the light treatments but did not hamper the growth of the macrophytes. These results indicate that LED light supplementation enables the growth of V. natans under eutrophic conditions, at least in the absence of fish as in our experiment, and that the method may have potential as a restoration method in urban lakes. Lake-scale studies are needed, however, to fully evaluate LED light supplementation under natural conditions where other stressors (e.g., fish grazing) may need to be controlled for successful restoration of urban lakes. [Display omitted] • Light addition could enable macrophyte growth in eutrophic waters. • Light addition did not promote growth and shading effects of phytoplankton. • Light addition enabled periphyton growth, but with little shading effects on plants. [ABSTRACT FROM AUTHOR]

Along with a steady increasing use of artificial nitrogen fertilizer, concerns have been raised about the effects that high nitrogen loading may have on ecosystems. Due to the toxicity of unionized ammonia (NH 3), tolerance criteria have been proposed for ambient water management in many countries; however, these are mainly based on acute or chronic tests carried out under lab conditions run with purified water. Aiming at understanding the responses of organisms to natural exposure to high ammonia concentrations, a Viviparidae gastropod, Bellamya aeruginosa , was tested at three experimental scales: standard 96-h lab test, one-month cage test in 6 experimental ponds with continuous nitrogen inputs, and intensive investigation of the B. aeruginosa from these ponds in spring and winter. The results were: 1) 96-h LC 50 in the standard lab test was 0.56 mg NH 3 -N/L and 343.3 mg TAN/L (total ammonia expressed as N, standardized at pH 7 and 20 ℃). 2) In the one-month cage test, the survival rate was 97% when NH 3 -N was 0.61 mg/L (i.e., a higher concentration than the lab 96-h LC 50) and the body size of the gastropods actually increased with increasing NH 3 -N concentrations. 3) In the winter-spring investigation, little effect of ammonia on the standing crops of gastropods was found, and the body size of the gastropods tended to increase with increasing ammonia concentrations (NH 3 -N concentration range of 0.05 ~ 2.06 mg/L). Thus, B. aeruginosa showed higher tolerance to ammonia exposure (NH 3 -N concentration < 0.81 mg/L) in the field than under laboratory conditions. Our study points to the necessity of considering the relevant scale when determining criteria for ammonia toxicity in water management. • B. aeruginosa were more tolerant to ammonia in field than in laboratory. • B. aeruginosa had higher survival rates and grew larger in field than in laboratory. • Food availability and shelter from sediments released B. aeruginosa from ammonia exposure. [ABSTRACT FROM AUTHOR]

Abstract: Located in the Yunnan–Guizhou Plateau in southwest China, Fuxian Lake covers an area of 211km2, with maximum depth of 155m. It is known to have a unique fauna, including 14 described endemic species. In order to describe the zoobenthic community of the lake more completely, the present study was conducted from August 2002 to August 2003. Altogether 62 benthic taxa, including 22 oligochaetes, 21 molluscs and 18 insects were identified, of which the dominant taxa belonged to Potamothrix, Procladius and Paraprososthenia. The standing stocks of benthos were much higher in the littoral (824ind/m2 in density, 3.72g/m2 in biomass) than in the profundal region (23ind/m2 in density, 0.10g/m2 in biomass). Species richness was greatest in summer and standing stocks were larger in spring and summer than in other seasons. Analyses of functional feeding groups indicated that collector-gatherers and scrapers were predominant in entire lake. Stepwise multiple regression analysis demonstrated that the water depth is the most important factor affecting the distribution of macrozoobenthos. [Copyright &y& Elsevier]

Abstract: The farming of Chinese mitten crab, a quality aquatic product in China and neighbouring Asian countries, has been developing rapidly in China since last decade. It reached a total yield of 3.4×105 tonnes in 2002. Due to the successive over-stocking year after year, many lakes in the mid-lower Yangtze Basin, the main farming area, are under deterioration, leading to a reduction of crab yield and quality, and, subsequently, a loss of farming profits. Aiming at a normal development of crab culture and the sustainable use of lakes, an annual investigation dealing with lake environmental factors in relation to stocked crab populations was carried out at 20 farms in 4 lakes. The results show that the submersed macrophyte biomass (B Mac) is the key factor affecting annual crab yield (CY). Using the ratio of Secchi depth to mean depth (Z SD / Z M), an easily measured parameter closely correlated to B Mac, as driving variable, 10 regression models of maximal crab yields were generated (r 2 ranging 0.49–0.81). Based on the theory of MSY (Maximum Sustainable Yield), in combination with body-weight (BW) and recapture rate (RR) of adult crabs, a general optimal stocking model was eventually formulated. All models are simple and easy to operate. Comments on their applications and prospects are given in brief. [Copyright &y& Elsevier]

Abstract: Submersed macrophytes in Yangtze lakes have experienced large-scale declines due to the increasing human activities during past decades. To seek the key factor that affects their growth, monthly investigations of submersed macrophytes were conducted in 20 regions of four Yangtze lakes during December, 2001–March, 2003. Analyses based on annual values show that the ratio of Secchi depth to mean depth is the key factor (50% of macrophyte biomass variability among these lakes is statistically explained). Further analyses also demonstrate that the months from March to June are not only the actively growing season for most macrophytes, but the key time the factor acts. Five key-time models yielding higher predictive power (r 2 reaches 0.75, 0.76, 0.77, 0.69 and 0.81) are generated. A comparison between key-time models and traditional synchronic ones indicates that key-time models have higher predictive power. Analyses of transparency thresholds during macrophyte growing season and the limitations of the models are presented. The models and other results may benefit the work concerning submersed macrophyte recovery in Yangtze lakes. [Copyright &y& Elsevier]

Effects of Artificial LED Light on the Growth of Three Submerged Macrophyte Species during the Low-Growth Winter Season: Implications for Macrophyte Restoration in Small Eutrophic Lakes. University of Chinese Academy of Sciences, Beijing 100049, China. 1 Xu C., Wang H.-J., Yu Q., Wang H.-Z., Liang X.-M., Liu M., Jeppesen E. Effects of Artificial LED Light on the Growth of Three Submerged Macrophyte Species during the Low-Growth Winter Season: Implications for Macrophyte Restoration in Small Eutrophic Lakes. [Extracted from the article]

The management of water levels in wetlands is of great importance for the wetland ecosystem, including the conservation and revitalization of plants. However, the water level requirements (WLRs) of wetland plants have not been well investigated. In this study, Typha angustifolia was selected as an experimental plant species. Combining field investigation and simulation experiments, the relationship between the development status of this species and water level fluctuations (WLFs) in different life-history stages were analyzed. The results show that populations in the Yangtze floodplain, China, had two phenotypic forms 'tall' and 'short', and that these were distributed in lakes with intermittent or quasi-natural fluctuations and reservoir-like fluctuations, respectively. Lakes with high amplitude (>3.2 m) water fluctuations did not contain T. angustifolia. We investigated the distribution and growth of T. angustifolia in lakes of varying hydrology across the Yangtze floodplain, seeking to define its tolerance of water-level fluctuations and submergence at different stages in its life cycle. The upper tolerance limit of static submerged water depth was bounded by 1.5 times the height of plants in the seedling stage, and the upper tolerance limit of the submergence rate in the seedling stage was the average growth rate of seedling, 1.5 cm/d. The plant height had a positive linear correlation with amplitude and water depth from June to July. The autumn biomass was significantly negatively correlated with amplitude and water depth from January to May. This paper is perhaps the first case study on water level fluctuation requirements (WLFRs) of emergent macrophytes. It systematically assessed the WLFRs of T. angustifolia in each life-history stage, and established a comprehensive WLFR conceptual model. The results of this study could provide a quantitative operational basis for the protection and restoration of this species in Yangtze floodplain lakes. [ABSTRACT FROM AUTHOR]

Eutrophication of lakes is becoming a global environmental problem, leading to, among other things, rapid reproduction of phytoplankton, increased turbidity, loss of submerged macrophytes, and the recovery of these plants following nutrient loading reduction is often delayed. Artificial light supplement could potentially be a useful method to help speeding up recovery. In this study, three common species of submerged macrophytes, Vallisneria natans, Myriophyllum spicatum and Ceratophyllum demersum, were exposed to three LED light treatments (blue, red and white) and shaded (control) for 100 days (from 10 November 2016 to 18 January 2017) in 12 tanks holding 800 L of water. All the three LED light treatments promoted growth of the three macrophyte species in terms of shoot number, length and dry mass. The three light treatments differed in their effects on the growth of the plants; generally, the red light had the strongest promoting effects, followed by blue and white. The differences in light effects may be caused by the different photosynthetic photon flux density (PPFD) of the lights, as indicated by an observed relationship of PPFD with the growth variables. The three species also responded differently to the light treatments, V. natans and C. demersum showing higher growth than M. spicatum. Our findings demonstrate that artificial light supplement in the low-growth winter season can promote growth and recovery of submerged macrophytes and hence potentially enhance their competitiveness against phytoplankton in the following spring. More studies, however, are needed to elucidate if LED light treatment is a potential restoration method in small lakes, when the growth of submerged macrophytes are delayed following a sufficiently large external nutrient loading reduction for a shift to a clear macrophyte state to have a potential to occur. Our results may also be of relevance when elucidating the role of artificial light from cities on the ecosystem functioning of lakes in urban areas. [ABSTRACT FROM AUTHOR]

Dolman (2014) argued that our recent finding (Wang et al., 2014) of no marked effect of submerged macrophytes on the chlorophyll a (Chl a) to total phosphorus ( TP) relationship in Yangtze subtropical lakes may not necessarily be valid due to the statistical procedure used to analyse the relationship between these two variables., We agree with Dolman (2014) that a likelihood ratio test could provide an alternative statistical approach to analyse the effect of macrophytes on Chl a versus TP. However, we do not consider it would be appropriate to use the test on only a limited sub-data set corresponding to where TP values overlap for the vegetated and non-vegetated states, as suggested by Dolman (2014)., Besides testing slopes of the log10(Chl a)-log10( TP) relationship, we compared log10(Chl a)/log10( TP) values between vegetated and non-vegetated lakes at a range of overlapping TP concentrations (Wang et al., 2014)., The conclusion drawn by Dolman (2014) from a highly cited work on subtropical lakes in Florida was shown to be invalid. Re-analysis of these lakes demonstrated similar results to those found in Yangtze subtropical lakes. We therefore consider that our original conclusions are valid and that submersed macrophytes in subtropical lakes do not necessarily exert the same strong effects on algal turbidity as in temperate lakes. [ABSTRACT FROM AUTHOR]

Abstract In aquatic ecosystems, ammonium is one of the dominant substances in the effluent discharge from wastewater treatment plants and its impact has been widely explored as it is thought, in its toxic form (NH 3), to cause stress on organisms. Little is, however, known about its potential effect on the release of phosphorus (P) from the sediment. In a two-month mesocosm (150 L) experiment, we tested if high loading of ammonium promotes sediment P release and investigated the dominant underlying mechanisms. A gradient of five target ammonium loading levels was used by adding NH 4 Cl fertilizer: no addition/control (N0), 3 (N1), 5 (N2), 10 (N3), and 21 (N4) mg NH 4 Cl L−1 (NH 4 Cl expressed as nitrogen). We found that: 1) significant sediment P release for N3 and N4 but minor release or retention for N0, N1, and N2 were detected both by the total phosphorus concentration (TP) in the overlying water and in situ measurements of diffusive gradients in thin-films (DGT) at the sediment-water interface; 2) overall, TP correlated significantly and positively with total nitrogen (TN) concentrations in the water. Further correlation and path analyses suggested that stimulated alkaline phosphatase activity (APA) was likely the dominant mechanisms behind the ammonium-induced sediment P release and decreased dissolved oxygen (DO) levels (an approximate reduction from 9.2 to 6.6 mg O2 L−1) was likely a contributing factor, particularly in the beginning of the experiment. Graphical abstract Image Highlights • High ammonium loading promoted sediment phosphorus release. • High ammonium increased alkaline phosphatase activity and decreased oxygen. • Phosphorus release was caused mainly by increased alkaline phosphatase activity. [ABSTRACT FROM AUTHOR]

Loss of submersed macrophytes is a world-wide phenomenon occurring when shallow lakes become eutrophic due to excess nutrient loading. In addition to the well-known effect of phosphorus, nitrogen as a trigger of macrophyte decline has received increasing attention. The precise impact of high nitrogen concentrations is debated, and the role of different candidate factors may well change over the season. In this study, we conducted experiments with Vallisneria natans during the growing season (June–September) in 10 ponds subjected to substantial differences in nitrogen loading (five targeted total nitrogen concentrations: control, 2, 10, 20, and 100 mg L − 1 ) and compared the results with those obtained in our earlier published study from the low-growth season (December–April). Like in the low-growth season, growth of V. natans in summer declined with increasing ammonium (NH 4 ) concentrations and particularly with increasing phytoplankton chlorophyll a (Chl a Phyt ). Accordingly, we propose that shading by phytoplankton might be of key importance for macrophyte decline, affecting also periphyton growth as periphyton chlorophyll a (Chl a Peri ) decreased with increasing Chl a Phyt . Free amino acid contents (FAA) of plants tended to increase with increasing NH 4 concentrations, while the relationships between FAA with growth indices were all weak, suggesting that FAA might be a useful indicator of the physiological stress of the plants but not of macrophyte growth. Taken together, the results from the two seasons indicate that although a combination of high nitrogen concentrations (ammonium) and shading by phytoplankton may cause severe stress on macrophytes, active growth in the growing season enabled them to partly overcome the stress. [ABSTRACT FROM AUTHOR]

A number of studies have revealed ammonia to be toxic to aquatic organisms; however, little is known about its effects under natural conditions. To elucidate the role of ammonia, we conducted 96-h acute toxicity tests as well as a whole-ecosystem chronic toxicity test for one year in ten 600-m 2 ponds. Three common cyprinids, silver carp Hypophthalmichthys molitrix Val. ( H.m. ), bighead carp Aristichthys nobilis Richardson ( A.n. ), and gibel carp Carassius auratus gibelio Bloch ( C.g. ), were used as test organisms. The 96-h LC 50 values of un-ionized ammonia (NH 3 ) for H.m. , A.n. , and C.g. were 0.35, 0.33, and 0.73 mg L − 1 , respectively. In the ponds, annual mean NH 3 ranged between 0.01 and 0.54 mg L − 1 , with 4 ponds having a NH 3 higher than the LC 50 of A.n. (lowest LC 50 in this study). No fish were found dead in the high-nitrogen ponds, but marked histological changes were found in livers and gills. Despite these changes, the specific growth rate of H.m. and A.n. increased significantly with NH 3 . Our pond results suggest that fish might be more tolerant to high ammonia concentrations in natural aquatic ecosystems than under laboratory conditions. Our finding from field experiments thus suggests that the existing regulatory limits for reactive nitrogen (NH 3 ) established from lab toxicity tests might be somewhat too high at the ecosystem conditions. Field-scale chronic toxicity tests covering full life histories of fish and other aquatic organisms are therefore encouraged in order to optimize determination of the effects of ammonia in natural environments. [ABSTRACT FROM AUTHOR]

The Chinese mitten crab ( Eriocheir sinensis (H. Milne Edwards, 1854)), is a highly invasive species and poses a great threat to endemic species and infrastructure in Europe and North America. Although it is partly herbivorous and prefers to live in lakes with abundant submersed macrophytes, little is known about its effect on macrophytes. We used its native range, the mid-lower Yangtze Basin where the species has been cultured intensively for decades, as our study site to test the hypotheses that (1) high crab densities weaken the positive feedback between macrophytes and water transparency, and that (2) the effects of crabs become apparent only on decadal timescales and (3) are density dependent. We used correlative analyses based on 12 years of monitoring and multi-lake comparisons among 20 sub-areas in 4 lakes. High crab densities were found to cause negative effects on submersed macrophytes and transparency, and to weaken the positive relation between macrophytes and transparency. High densities of macrophytes showed resilience to disturbance from crabs. This resilience, however, reduced with continuous presence of high crab densities. Crab densities were strongly positively related with total phosphorus and negatively with transparency and total nitrogen. Phosphorus concentrations and transparency were not related with phytoplankton chlorophyll a , suggesting that crab’s bioturbation strongly influences water quality. The apparent resilience of the dense macrophyte stands should however, not delay attempts to eradicate the crab where it is invasive as this becomes more difficult once they have become established. When macrophyte abundance is already low at the time of invasion, immediate loss of macrophytes may occur. [ABSTRACT FROM AUTHOR]