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The job shop scheduling problem (JSSP) is a fundamental operational research topic with numerous applications in the real world. Since the JSSP is an NP-hard (nondeterministic polynomial time) problem, approximation approaches are frequently used to rectify it. This study proposes a novel biologically-inspired metaheuristic method named Coral Reef Optimization in conjunction with two local search techniques, Simulated Annealing (SA) and Variable Neighborhood Search (VNS), with significant performance and finding-solutions speed enhancement. The two-hybrid algorithms’ performance is evaluated by solving JSSP of various sizes. The findings demonstrate that local search strategies significantly enhance the search efficiency of the two hybrid algorithms compared to the original algorithm. Furthermore, the comparison results with two other metaheuristic algorithms that also use the local search feature and five state-of-the-art algorithms found in the literature reveal the superior search capability of the two proposed hybrid algorithms.

DDoS (Distributed Denial of Service) has emerged as a serious and challenging threat to computer networks and information systems’ security and integrity. Before any remedial measures can be implemented, DDoS assaults must first be detected. DDoS attacks can be identified and characterized with satisfactory achievement employing ML (Machine Learning) and DL (Deep Learning). However, new varieties of aggression arise as the technology for DDoS attacks keep evolving. This research explores the impact of a new incarnation of DDoS attack–adversarial DDoS attack. There are established works on ML-based DDoS detection and GAN (Generative Adversarial Network) based adversarial DDoS synthesis. We confirm these findings in our experiments. Experiments in this study involve the extension and application of the GAN, a machine learning framework with symmetric form having two contending neural networks. We synthesize adversarial DDoS attacks utilizing Wasserstein Generative Adversarial Networks featuring Gradient Penalty (GP-WGAN). Experiment results indicate that the synthesized traffic can traverse the detection systems such as k-Nearest Neighbor (KNN), Multi-Layer Perceptron (MLP) and Random Forest (RF) without being identified. This observation is a sobering and pessimistic wake-up call, implying that countermeasures to adversarial DDoS attacks are urgently needed. To this problem, we propose a novel DDoS detection framework featuring GAN with Dual Discriminators (GANDD). The additional discriminator is designed to identify adversary DDoS traffic. The proposed GANDD can be an effective solution to adversarial DDoS attacks, as evidenced by the experimental results. We use adversarial DDoS traffic synthesized by GP-WGAN to train GANDD and validate it alongside three other DL technologies: DNN (Deep Neural Network), LSTM (Long Short-Term Memory) and GAN. GANDD outperformed the other DL models, demonstrating its protection with a TPR of 84.3%. A more sophisticated test was also conducted to examine GANDD’s ability to handle unseen adversarial attacks. GANDD was evaluated with adversarial traffic not generated from its training data. GANDD still proved effective with a TPR around 71.3% compared to 7.4% of LSTM.

DDoS (Distributed Denial of Service) attacks have become a pressing threat to the security and integrity of computer networks and information systems, which are indispensable infrastructures of modern times. The detection of DDoS attacks is a challenging issue before any mitigation measures can be taken. ML/DL (Machine Learning/Deep Learning) has been applied to the detection of DDoS attacks with satisfactory achievement. However, full-scale success is still beyond reach due to an inherent problem with ML/DL-based systems—the so-called Open Set Recognition (OSR) problem. This is a problem where an ML/DL-based system fails to deal with new instances not drawn from the distribution model of the training data. This problem is particularly profound in detecting DDoS attacks since DDoS attacks’ technology keeps evolving and has changing traffic characteristics. This study investigates the impact of the OSR problem on the detection of DDoS attacks. In response to this problem, we propose a new DDoS detection framework featuring Bi-Directional Long Short-Term Memory (BI-LSTM), a Gaussian Mixture Model (GMM), and incremental learning. Unknown traffic captured by the GMM are subject to discrimination and labeling by traffic engineers, and then fed back to the framework as additional training samples. Using the data sets CIC-IDS2017 and CIC-DDoS2019 for training, testing, and evaluation, experiment results show that the proposed BI-LSTM-GMM can achieve recall, precision, and accuracy up to 94%. Experiments reveal that the proposed framework can be a promising solution to the detection of unknown DDoS attacks.

Summary: Autoimmunity in plants has been found in numerous hybrids as a form of hybrid necrosis and mutant panels. Uncontrolled cell death is a main cellular outcome of autoimmunity, which negatively impacts growth. Its occurrence highlights the vulnerable nature of the plant immune system. Genetic investigation of autoimmunity in hybrid plants revealed that extreme variation in the immune receptor repertoire is a major contributor, reflecting an evolutionary conundrum that plants face in nature. In this review, we discuss natural variation in the plant immune system and its contribution to fitness. The value of autoimmunity genetics lies in its ability to identify combinations of a natural immune receptor and its partner that are predisposed to triggering autoimmunity. The network of immune components for autoimmunity becomes instrumental in revealing mechanistic details of how immune receptors recognize cellular invasion and activate signaling. The list of autoimmunity‐risk variants also allows us to infer evolutionary processes contributing to their maintenance in the natural population. Our approach to autoimmunity, which integrates mechanistic understanding and evolutionary genetics, has the potential to serve as a prognosis tool to optimize immunity in crops. [ABSTRACT FROM AUTHOR]

• Activation of immune responses requires receptor complex assembly. • Receptor complexes differentially reside in regulatory scaffolds. • Phytocytokines, such as RALF peptides, can positively/negatively regulate immunity. • Appropriate immune responses are tightly controlled at multiple levels. • Stacked immune receptors may be used to engineer broad-spectrum disease resistance. Plant plasma membrane pattern recognition receptors are key to microbe sensing and activation of immunity to microbial invasion. Plants employ several types of such receptors that differ mainly in the structure of their ectodomains and the presence or absence of a cytoplasmic protein kinase domain. Plant immune receptors do not function as single entities, but form larger complexes which undergo compositional changes in a ligand-dependent manner. Here, we highlight current knowledge of molecular mechanisms underlying receptor complex dynamics and regulation, and cover early signaling networks implicated in the activation of generic plant immune responses. We further discuss how an increasingly comprehensive set of immune receptors may be employed to engineer crop plants with enhanced, durable resistance to microbial infection. [ABSTRACT FROM AUTHOR]

Summary: Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine‐rich repeat receptor kinases (LRR‐RK) FLS2 and EFR, and the LRR receptor protein (LRR‐RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families.We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR‐RK and LRR‐RP signaling.Quantitative differences in timing and amplitude were observed for several early immune responses, with RP‐mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23‐regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2‐signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP‐type immune receptors in a manner dependent on BIK1 kinase activity.Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity. [ABSTRACT FROM AUTHOR]

Abstract: Inflammation is involved in many human pathologies, including osteoarthritis (OA). Hydrogen (H2) is known to have anti‐inflammatory effects; however, the bioavailability of directly administered H2 gas is typically poor. Herein, a local delivery system that can provide a high therapeutic concentration of gaseous H2 at inflamed tissues is proposed. The delivery system comprises poly(lactic‐co‐glycolic acid) microparticles that contain magnesium powder (Mg@PLGA MPs). Mg@PLGA MPs that are intra‐muscularly injected close to the OA knee in a mouse model can act as an in situ depot that can evolve gaseous H2 continuously, mediated by the cycle of passivation/activation of Mg in body fluids, at a concentration that exceeds its therapeutic threshold. The analytical data that are obtained in the biochemical and histological studies indicate that the proposed Mg@PLGA MPs can effectively mitigate tissue inflammation and prevent cartilage from destruction, arresting the progression of OA changes. [ABSTRACT FROM AUTHOR]

Hydroxyproline-rich glycopeptides (HypSys) are small signalling peptides containing 18-20 amino acids. The expression of IbpreproHypSys, encoding the precursor of IbHypSys, was induced in sweet potato ( Ipomoea batatas cv. Tainung 57) through wounding and IbHypSys treatments by using jasmonate and H2O2. Transgenic sweet potatoes overexpressing (OE) and silencing [RNA interference (RNAi)] IbpreproHypSys were created. The expression of the wound-inducible gene for ipomoelin ( IPO) in the local and systemic leaves of OE plants was stronger than the expression in wild-type (WT) and RNAi plants after wounding. Furthermore, grafting experiments indicated that IPO expression was considerably higher in WT stocks receiving wounding signals from OE than from RNAi scions. However, wounding WT scions highly induced IPO expression in OE stocks. These results indicated that IbpreproHypSys expression contributed towards sending and receiving the systemic signals that induced IPO expression. Analysing the genes involved in the phenylpropanoid pathway demonstrated that lignin biosynthesis was activated after synthetic IbHypSys treatment. IbpreproHypSys expression in sweet potato suppressed Spodoptera litura growth . In conclusion, wounding induced the expression of IbpreproHypSys, whose protein product was processed into IbHypSys. IbHypSys stimulated IbpreproHypSys and IPO expression and enhanced lignin biosynthesis, thus protecting plants from insects. [ABSTRACT FROM AUTHOR]

Leroux et al. haben Experimente aus unserer Studie wiederholt und berichten, dass sie das thermoresponsive liposomale System mit Ammoniumbicarbonat (ABC), für das wir Wirkstofffreisetzung unter mildem Erwärmen demonstrierten, nicht reproduzieren konnten. Wir widersprechen ihrer Beurteilung und spekulieren, dass die andersartige Methode, die Leroux et al. für die Herstellung des wässrigen ABC verwendeten, zu dem negativen Ergebnis führte. [ABSTRACT FROM AUTHOR]

Plants have evolved effective strategies to defend themselves against pathogen invasion. Starting from the plasma membrane with the recognition of microbe-associated molecular patterns (MAMPs) via pattern recognition receptors, internal cellular signaling pathways are induced to ultimately fend off the attack. Phospholipase D (PLD) hydrolyzes membrane phospholipids to produce phosphatidic acid (PA), which has been proposed to play a second messenger role in immunity. The Arabidopsis ( Arabidopsis thaliana ) PLD family consists of 12 members, and for some of these, a specific function in resistance toward a subset of pathogens has been shown. We demonstrate here that Arabidopsis PLDγ1, but not its close homologs PLDγ2 and PLDγ3, is specifically involved in plant immunity. Genetic inactivation of PLDγ1 resulted in increased resistance toward the virulent bacterium Pseudomonas syringae pv. tomato DC3000 and the necrotrophic fungus Botrytis cinerea As pldγ1 mutant plants responded with elevated levels of reactive oxygen species to MAMP treatment, a negative regulatory function for this PLD isoform is proposed. Importantly, PA levels in pldγ1 mutants were not affected compared to stressed wild-type plants, suggesting that alterations in PA levels are not likely the cause for the enhanced immunity in the pldγ1 line. Instead, the plasma-membrane-attached PLDγ1 protein colocalized and associated with the BAK1-INTERACTING RECEPTOR-LIKE KINASES BIR2 and BIR3, which are known negative regulators of pattern-triggered immunity. Moreover, complex formation of PLDγ1 and BIR2 was further promoted upon MAMP treatment. Hence, we propose that PLDγ1 acts as a negative regulator of plant immune responses in complex with immunity-related proteins BIR2 and BIR3., (© 2020 American Society of Plant Biologists. All Rights Reserved.)

Inflammation is involved in many human pathologies, including osteoarthritis (OA). Hydrogen (H 2 ) is known to have anti-inflammatory effects; however, the bioavailability of directly administered H 2 gas is typically poor. Herein, a local delivery system that can provide a high therapeutic concentration of gaseous H 2 at inflamed tissues is proposed. The delivery system comprises poly(lactic-co-glycolic acid) microparticles that contain magnesium powder (Mg@PLGA MPs). Mg@PLGA MPs that are intra-muscularly injected close to the OA knee in a mouse model can act as an in situ depot that can evolve gaseous H 2 continuously, mediated by the cycle of passivation/activation of Mg in body fluids, at a concentration that exceeds its therapeutic threshold. The analytical data that are obtained in the biochemical and histological studies indicate that the proposed Mg@PLGA MPs can effectively mitigate tissue inflammation and prevent cartilage from destruction, arresting the progression of OA changes., (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Hydrogen gas can reduce cytotoxic reactive oxygen species (ROS) that are produced in inflamed tissues. Inspired by natural photosynthesis, this work proposes a multicomponent nanoreactor (NR) that comprises chlorophyll a, l-ascorbic acid, and gold nanoparticles that are encapsulated in a liposomal (Lip) system that can produce H 2 gas in situ upon photon absorption to mitigate inflammatory responses. Unlike a bulk system that contains free reacting molecules, this Lip NR system provides an optimal reaction environment, facilitating rapid activation of the photosynthesis of H 2 gas, locally providing a high therapeutic concentration thereof. The photodriven NR system reduces the degrees of overproduction of ROS and pro-inflammatory cytokines both in vitro in RAW264.7 cells and in vivo in mice with paw inflammation that is induced by lipopolysaccharide (LPS). Histological examinations of tissue sections confirm the ability of the NR system to reduce LPS-induced inflammation. Experimental results indicate that the Lip NR system that can photosynthesize H 2 gas has great potential for mitigating oxidative stress in tissue inflammation.

Inflammation is associated with many diseases, in which activated inflammatory cells produce various reactive oxygen species (ROS), including H2O2. This work proposes an ultrasensitive ROS-responsive hollow microsphere (HM) carrier that contains an anti-inflammatory drug, an acid precursor consisting of ethanol and FeCl2, and sodium bicarbonate (SBC) as a bubble-generating agent. In cases of inflamed osteoarthritis, the H2O2 at low concentration diffuses through the HMs to oxidize their encapsulated ethanol in the presence of Fe(2+) by the Fenton reaction, establishing an acidic milieu. In acid, SBC decomposes to form CO2 bubbles, disrupting the shell wall of the HMs and releasing the anti-inflammatory drug to the problematic site, eventually protecting against joint destruction. These results reveal that the proposed HMs may uniquely exploit biologically relevant concentrations of H2O2 and thus be used for the site-specific delivery of therapeutics in inflamed tissues.