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Purpose: Floral waste, as a frequently overlooked resource for sustainable agriculture, can be harnessed to produce nutrient-rich soil amendments resulting in an enhancement of soil fertility and augment crop yields. This in turn can foster a circular economy, minimizing waste disposal and promoting a green economy. This study was aimed to explore the potential of rose distillation waste and other organic amendments as sustainable alternatives to traditional synthetic chemicals, particularly in the context of rose production. Method: The data was collected experimentally in a controlled greenhouse condition. A complete randomized block design with three replicates was applied to conduct the experimental units. The study investigated the combined effects of three organic amendments: goat manure rose distillation waste, and compost, as well as a bio-stimulant containing both Mycorrhizae and Plant Growth Promoting Rhizobacteria. Several parameters, including growth traits and leaves nutrient content, were assessed. Results: Rose distillation waste proved to be a promising organic amendment with a significant positive effect on the growth and development of seedlings. Rose distillation waste substrate showed a remarkable increase in terms of leaf area and a maximum apical internode growth of 1.5 mm. The incorporation of bio-stimulant in the rose distillation waste substrate resulted in significant stem height growth of 11.3 cm and a high bud break rate of 73.3%. Conclusion: The findings of the study offer valuable insights into the sustainable management of rose production. The use of rose distillation waste as an organic amendment not only enhances plant growth but also positively impacts nutrient content in leaves. This highlights the potential of rose distillation waste as a sustainable and easily available resource for use in organic agriculture. Highlights Rose distillation waste is reutilized as a sustainable soil amendment, enhancing soil fertility and bolstering crop yields, contributing to a more eco-friendly and circular economy. Both rose distillation waste and organic amendments are proven to be effective alternatives to synthetic chemicals in the cultivation of roses. Experiments conducted in greenhouses demonstrate significant growth advantages when combining rose distillation waste with a biostimulant rich in Mycorrhizae and Plant Growth Promoting Rhizobacteria. This study underscores the significance of employing rose distillation waste in cost-effective, sustainable, and easily accessible organic agricultural practices for rose production

In this study, 17 Trichoderma strains were isolated from different soils (crop fields and Argan forests) in Morocco. Purified monospore cultures were identified using molecular methods and tested for their potential antagonism against three phytopathogenic fungi (Fusarium oxyxporum, verticillium dahlia and rhizoctonia solani). After DNA extraction, translation elongation factor (tef1) was amplified in extracts of 17 strains, sequenced and compared with their ex-types. As a result, three species were identified among the strains, which clustered in two different subclades of Trichoderma: the species T. afroharzianum, and T. guizhouense belong to the Harzianum clade, while T. longibrachiatum belongs to the Longibrachiatum clade. Investigation of potential antagonistic effects of these strains against the soil-borne phytopathogens F. oxysporum, R. solani and V. dahliae was conducted in a dual culture plate assay, using 17 promising Trichoderma strains that have been selected based on a polymerase chain reaction (PCR) screening approach. In vitro, Trichoderma isolates showed effective antagonistic performance by decreasing soil borne pathogens mycelium radial growth. Trichoderma afroharzianum showed the highest Percentage of Radial Inhibition Growth (PRIG %). The highest PRIG% = 98% was for 8A2.3 isolate against R. solani and the lowest PRIG%= 67% for T9i10 against F. oxysporum. On the other hand, T9i12, which is T. reesei species, led to a high radial inhibition of pathogens’ mycelium.

Dans cette étude, 17 souches de Trichoderma on été isolées à partir d’échantillons du sol de différentes origines (champs et forêt d’arganier). Les espèces ont été identifiées par la caractérisation moléculaire des cultures monospores des isolats de Trichoderma et leur potentiel d’antagonisme a été évalué contre trois champignons phyto-pathogènes telluriques (Fusarium oxyxporum, verticillium dahlia et rhizoctonia solani). Après l’extraction de l’ADN, le facteur d’élongation et de traduction tef1 a été utilisé comme marqueur moléculaire pour amplifier, séquencer et par la suite caractériser les 17 souches qui ont été comparées avec leur ex-types déjà référencés. Par conséquent, l’identification moléculaire par tef1 a permis la caractérisation de trois espèces de Trichoderma à savoir T. afroharzianum, et T. guizhouense appartenant à la branche Harzianum, et T. longibrachiatum appartenant à la branche Longibrachiatum. Les 17 isolats candidats ont été sélectionnés par une méthode de criblage basée sur la PCR (polymerase chain reaction). L’évaluation du potentiel antagoniste des souches de Trichoderma contre les champignons phytopathogènes telluriques (F. oxysporum, R. solani et V. dahliae) a été réalisée par la méthode de confrontation sur boîtes de pétrie. L’étude in vitro de l’activité antagoniste par le «Pourcentage of Radial Inhibition Growth» (PRIG %) a permis de suivre l’évolution de la croissance radiale du mycélium des différents champignons phytopathogènes. Le pourcentage le plus élevé (PRIG% = 98%) a été observé pour la confrontation entre l’isolat 8A2.3 et R. solani et le pourcentage le plus faible (PRIG% = 67%) a été observé pour la confrontation entre l’isolat T9i10 et F. oxysporum. D’un autre coté, T9i12 qui est un isolat de l’espèce T. reeseia a engendré une inhibition élevée de la croissance radiale du mycélium des différents pathogènes. Mots clés: Trichoderma spp, Fusarium oxysporum, Verticillium dahlia, Rhizoctonia solani, champignons antagonistes.