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Cuci Tangan Pakai Sabun (CTPS) merupakan perilaku sederhana namun dapat memberikan manfaat bagi pencegahan penyakit menular. Pendekatan RANAS (Risk, Atitude, Norms, ability dan self arrangement) merupakan model baru untuk promosi perubahan perilaku masyarkaat. Untuk memperkenalkan metode RANAS, dilakukan pelatihan secara daring (dalam jaringan). Tujuannya meningkatkan pemahaman perserta latih tentang strategi perubahan perilaku dengan pendekatan RANAS. Sasaran pelatihan adalah sanitarian dan promotor kesehatan kabupaten maupun puskesmas di Kabuaten Belu dan TTU. Hasil evaluasi pelatihan menunjukkan pemahaman meningkat setelah dilakukan kegiatan pelatihan secara daring. Disarankan untuk melakukan evaluasi pasca pelatihan untuk mendapatkan informasi implementasi starategi RANAS dalam promosi perubahan perilaku., Cuci Tangan Pakai Sabun (CTPS) merupakan perilaku sederhana namun dapat memberikan manfaat bagi pencegahan penyakit menular. Pendekatan RANAS (Risk, Atitude, Norms, ability dan self arrangement) merupakan model baru untuk promosi perubahan perilaku masyarkaat. Untuk memperkenalkan metode RANAS, dilakuakn pelatihan secara daring (dalam jaringan). Tujuannya meningkatkan pemahaman perserta latih tentang strategi perubahan perilaku dengan pendekatan RANAS. Sasaran pelatihan adalah sanitarian dan promotor kesehatan kabupaten maupun puskesmas di Kabuaten Belu dan TTU. Hasil evaluasi pelatihan menunjukkan pemahaman meningkat setelah dilakukan kegiatan pelatihan secara daring. Disarankan untuk melakukan evaluasi pasca pelatihan untuk mendapatkan informasi implementasi starategi RANAS dalam promosi perubahan perilaku. &nbsp

Background: Organophosphate insecticides had been used in Kupang City of East Nusa Tenggara Province far before Kupang City was established in 1996. Determination of susceptibility status toward organophosphate insecticides i.e. temephos and malathion in Kupang City have never been conducted. This research purpose is to know Ae. aegypti larva susceptibility status from several villages in Kupang City of East Nusa Tenggara Province toward organophosphate insecticides, particularly temephos and malathion with the biological and biochemical test. Research Method: This is pure experimental research with The Posttest-Only Control Group Design for biological test and non-experimental tests with descriptive and analytical designs for the biochemical test. Material and method of a biological test are referred to test regulation by WHO (1996), whereas biochemical test is referred to Lee’s method (1991). Result: a Biological test using diagnostic dosage reveals that Ae. aegypti larva from several villages being tested has resistant (RR) status toward temephos with an average larva mortality rate of 99%. The result of qualitative and quantitative biochemical test shows that 3 villages have tolerant (SR) status with an average score of 2,0-2,5; 5 villages susceptible (SS) status with an average score of 0,900. The statistic analysis with Oneway ANOVA significant 5% rejects the null hypothesis (H0) which means that there has been a status decrease of Ae.aegypti mosquito larva susceptibility to organophosphate insecticides.Conclusion: Temephos insecticide is not relevant anymore to be used, and it is still relevant to use malathion in the Ae. aegypti mosquito control program in several villages in Kupang City of East Nusa Tenggara Province. Research result proves that susceptibility status decrease of tested mosquito larva in a biochemical test is known to be not caused by increasing activity nonspecific of esterase and acetylcholinesterase insensitivity, but may be caused by other the factors that have not been known.