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Background & Aims: Urinary tract infections (UTI) are a leading cause of bacterial infections in humans.The widespread use of antibiotics has resulted in the emergence of antibiotic-resistant microorganisms. Hence, the current study was conducted to investigate the antibiotic sensitivity pattern of uropathogens in UTI. Materials & Methods: A total of 642 urine samples were collected from suspected UTI patients and tested microbiologically. Antimicrobial susceptibility tests were performed for the isolated pathogens using the Kirby- Bauer disk diffusion method. Results: Out of 642 urine samples, 308 (48%) were found to exhibit significant bacteriuria. Females had a higher rate of UTI (68%) than males (32%), with a higher prevalence in the middle-aged group, while males reported a higher prevalence in the elderly group, which was statistically significant. The most common organism was Escherichia coli (57.2%), followed by Klebsiella pneumoniae (26.3%), Pseudomonas aeruginosa (8.4%), Proteus spp. (3.6%), Enterococcus spp. (2.6%), and Staphylococcus aureus (1.9%). UTI were more common in middle-aged female patients (31 to 45 years), while in males, high prevalence was seen in older patients (>45 years). Meropenem, Gentamicin, Nitrofurantoin, and Co-Trimoxazole were amongst the most sensitive drugs against E.coli and K.pneumoniae. Conclusion:Due to the irrational and injudicious use of antibiotics, commonly isolated uropathogens have a changing resistance pattern, resulting in reduced treatment effectiveness. This could be overcome by routine antimicrobial resistance surveillance, antimicrobial stewardship measures, and culture-guided therapy.

Abstract The sandalwood (Santalum album) tree holds significant commercial and cultural value globally, particularly in India. Among the 17 species within the Santalum genus, Santalum album stands out due to its high oil content and the presence of favorable compounds such as α-and β-santalols. The wood of this species is highly prized for carving and its aromatic heartwood oil is widely used in attar, perfumes, cosmetics, soaps, toiletries, medicinal products, flavored tobacco, and areca nut products. Despite its historical significance, sandalwood is vulnerable and endangered due to legal policies, illegal harvesting, grazing, lopping, natural fires, exotic plantations, sandalwood spike disease, and climate change. The combination of increased demand and declining supply has led to a surge in sandalwood prices, attracting attention from farmers, policymakers, and the industry sector, prompting interest in commercial cultivation. This has sparked global involvement from various countries, government agencies, industries, research disciplines, industry representatives, and sandalwood growers. This article offers a thorough review of sandalwood utilization, legal issues, and management challenges. It draws on literature and expert insights to assess current practices and research findings. The study identifies critical factors such as the number of hosts, host type, and planting geometry that influence successful establishment and production in farmers' fields. It also highlights obstacles and opportunities for the future sustainability of sandalwood, aiming to guide effective management and policy strategies for this valuable resource. The government of India has initiated policy-level changes regarding the ownership, cultivation, and harvesting of sandalwood trees, encouraging many farmers to cultivate on private land even beyond its natural boundaries. However, the complexity of sandalwood cultivation requires further scientific introspection to ensure successful establishment and sustainable management in the future.

Introduction: Acute encephalitis syndrome (AES) is one of the important causes of mortality among children in India. Active management of the cases, followed by addressing the cause of AES, is the key strategy for preventing mortality. Lack of laboratory facility and difficulty of sampling blood and cerebrospinal fluid (CSF) for assessing causes is one of the important barriers to early initiation of treatment. The main objective of the study is to validate the Scrub Typhus Encephalitis Assessment Tool (SEAT) for the management of AES. Methods: The study is a continuation of a study conducted in a tertiary care hospital in Eastern Uttar Pradesh. A machine learning (LightGBM) model was built to predict the probability of scrub typhus diagnosis among patients with acute encephalitis. Three models were built: one with sociodemographic characters, the second with Model 1 variables and blood parameters, and the third with Model 2 variables and CSF parameters. Results: The sensitivity of diagnosing the scrub typhus case was 71%, 77.5%, and 83% in Model 1, Model 2, and Model 3, respectively, and specificity was 61.5%, 75.5%, and 76.3%, respectively, in the models. In Model 1 fever duration, in Models 2 and 3, neutrophil/lymphocyte ratio was the most important predictor for differentiating the scrub and nonscrub cases. Conclusion: With the available sensitivity and specificity of the tool, the SEAT can be a valuable tool for the prediction of scrub typhus as a cause of AES cases in remote areas.

Abstract What nutrient supply options can ensure maximum productivity and optimize the nutrient uptake of rice–wheat system (R-W system)? an experiment started in the year 1998 (19-year-old) to examine the impact of optimal nutrient supply (NS) strategies to maximize crop productivity and nutritional security in R-W system. To determine the best nutrient management strategies (BNMS), seven different NS methods were tested. These included (organic and mineral fertilizers), as well as combinations such as integrated plant nutrition system (IPNS), IPNS + B (berseem), and IPNS + C (cowpea), with the aim of enhancing the productivity and nutrient absorption of R-W system. Results showed that rice grain yield notably wide-ranging from 1.61 to 5.81 t ha−1 under different NS options and highest rice grain yield (mean of 19 years) was observed at IPNS + C (5.81 t ha−1), which was at par with IPNS (5.79 t ha−1), STCR (soil test crop response) (5.76 t ha−1) and IPNS + B (5.67 t ha−1) followed by 100% recommended dose fertilizer NPK (4.41 t ha−1), which equality with OF (organic farming) (4.04 t ha−1) and lowest was recorded in control plot (1.61 t ha−1). Wheat grain yield varied significantly from 1.43 to 5.86 t ha−1 under different NS options. The highest yield (mean of 19 years) was observed in treatments IPNS + B (5.86 t ha−1), IPNS (5.77 t ha−1), IPNS + C (5.48 t ha−1), and STCR (5.45 t ha−1), followed by OF at 4.49 t ha−1, NPK at 3.76 t ha−1, with the lowest in the control plot at 1.43 t ha−1. Additionally, total phosphorus and sulfur accumulation in rice (grain: 8.41 to 39.09 kg ha−1; straw: 6.02 to 23.54 kg ha−1) varied significantly across nutrient supply treatments. Overall, adoption of IPNS integration with legumes (IPNS + B and IPNS + C), can significantly improve productivity and nutrient accumulation in R-W systems. Incorporating legumes into farming practices is advised for sustained productivity and nutritional benefits.

Abstract Background Lipids produced using oleaginous yeast cells are an emerging feedstock to manufacture commercially valuable oleochemicals ranging from pharmaceuticals to lipid-derived biofuels. Production of biofuels using oleaginous yeast is a multistep procedure that requires yeast cultivation and harvesting, lipid recovery, and conversion of the lipids to biofuels. The quantitative recovery of the total intracellular lipid from the yeast cells is a critical step during the development of a bioprocess. Their rigid cell walls often make them resistant to lysis. The existing methods include mechanical, chemical, biological and thermochemical lysis of yeast cell walls followed by solvent extraction. In this study, an aqueous thermal pretreatment was explored as a method for lysing the cell wall of the oleaginous yeast Rhodotorula toruloides for lipid recovery. Results Hydrothermal pretreatment for 60 min at 121 °C with a dry cell weight of 7% (w/v) in the yeast slurry led to a recovery of 84.6 ± 3.2% (w/w) of the total lipids when extracted with organic solvents. The conventional sonication and acid-assisted thermal cell lysis led to a lipid recovery yield of 99.8 ± 0.03% (w/w) and 109.5 ± 1.9% (w/w), respectively. The fatty acid profiles of the hydrothermally pretreated cells and freeze-dried control were similar, suggesting that the thermal lysis of the cells did not degrade the lipids. Conclusion This work demonstrates that hydrothermal pretreatment of yeast cell slurry at 121 °C for 60 min is a robust and sustainable method for cell conditioning to extract intracellular microbial lipids for biofuel production and provides a baseline for further scale-up and process integration. Graphical abstract

Led by a premise that effective cooperative tasks play a vital role in enhancing Cognitive Engagement (CE) and task achievement in ESL/EFL teaching, this study investigates the effects of Cooperative Learning (CL) on Omani Bachelor of Education (B. Ed.) students' CE and task achievement. This quasiexperimental study has three objectives: (i) To find out the level of awareness of CE skills required during EFL learning sessions; (ii) To examine whether there are any statistically significant differences between the mean scores of students' responses taught through CL strategies and taught in normal setting; and (iii) To investigate whether there are significant differences among EFL students' CE levels attributed to their gender. A total of 36 B. Ed. students were assigned to Experimental Group and Control Group with 18 students in each. Analytical results of the study found that (a) The overall CE levels of the students was low at the mean score of 2.20; (b) On statistical grounds, significant differences were found at the level of <0.01 between the mean scores of CE responses of students taught through CL and those who were taught in a normal setting; and (c) No statistically significant differences were found at the level of <0.05 between mean scores of students' CE responses attributed to their gender. We have discussed a number of pedagogical implications emerging from the findings of this study for raising students' awareness, enhancing teachers' roles and responsibilities, effective task designing and developing engaging instructional materials.

Abstract Oilcane—an oil‐accumulating crop engineered from sugarcane—and microbial oil have the potential to improve renewable oil production and help meet the expected demand for bioderived oleochemicals and fuels. To assess the potential synergies of processing both plant and microbial oils, the economic and environmental implications of integrating microbial oil production at oilcane and sugarcane biorefineries were characterized. Due to decreased crop yields that lead to higher simulated feedstock prices and lower biorefinery capacities, current oilcane prototypes result in higher costs and carbon intensities than microbial oil from sugarcane. To inform oilcane feedstock development, we calculated the required biomass yields (as a function of oil content) for oilcane to achieve financial parity with sugarcane. At 10 dw% oil, oilcane can sustain up to 30% less yield than sugarcane and still be more profitable in all simulated scenarios. Assuming continued improvements in microbial oil production from cane juice, achieving this target results in a minimum biodiesel selling price of 1.34 [0.90, 1.85] USD∙L−1 (presented as median [5th, 95th] percentiles), a carbon intensity of 0.51 [0.47, 0.55] kg CO2e L−1, and a total biodiesel yield of 2140 [1870, 2410] L ha−1 year−1. Compared to biofuel production from soybean, this outcome is equivalent to 3.0–3.9 as much biofuel per hectare of land and a 57%–63% reduction in carbon intensity. While only 20% of simulated scenarios fell within the market price range of biodiesel (0.45–1.11 USD∙L−1), if the oilcane biomass yield would improve to 25.6 DMT∙ha−1∙y−1 (an equivalent yield to sugarcane) 87% of evaluated scenarios would have a minimum biodiesel selling price within or below the market price range.

Abnormal accumulation of insoluble α-synuclein (α-Syn) inclusions in neurons, neurites, and glial cells is the defining neuropathology of synucleinopathies, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy. Accumulation of α-Syn inclusions in the amygdala has been well-documented in post-mortem studies of PD and DLB brains, as well as preclinical animal models of these conditions. Though α-Syn pathology is closely associated with neurodegeneration, there is a poor correlation between neuronal loss in the amygdala and the clinical features of PD and DLB. Moreover, functional interaction between the cerebral cortex and the amygdala is critical to regulating emotion, motivation, and social behaviors. The cortico-amygdala functional interaction is likely to be disrupted by the development of α-Syn pathology in the brain. Thus, we hypothesize that neuronal α-Syn inclusions disrupt cortical modulation of the amygdala circuits and are sufficient to drive social behavioral deficits. In the present work, we designed a series of longitudinal studies to rigorously measure the time courses of neurodegeneration, functional impairment of cortico-amygdala connectivity, and development of amygdala-dependent social behavioral deficits to test this hypothesis. We injected α-Syn preformed fibrils (PFFs) into the dorsal striatum to induce α-Syn aggregation in the amygdala and the medial prefrontal cortex (mPFC) of C57BL6 mice of both sexes, followed by a detailed analysis of temporal development of α-Syn pathology, synaptic deficits, and neuronal loss in the amygdala, as well as behavioral deficits at 3–12 months post injections. Development of α-Syn inclusions caused losses of cortical axon terminals and cell death in the basolateral amygdala (BLA) at 6- and 12-months post injections, respectively. At a relatively early stage of 3 months post injections, the connection strength of the mPFC-BLA synapse was decreased in PFFs-injection mice compared to controls. Meanwhile, the PFFs-injected mice showed impaired social interaction behavior, which was rescued by chemogenetic stimulation of mPFC-BLA connections. Altogether, we presented a series of evidence to delineate circuit events in the amygdala associated with the accumulation of α-Syn inclusions in the mouse brain, highlighting that functional impairment of the amygdala is sufficient to cause social behavior deficits. The present work further suggests that early circuit modulation could be an effective approach to alleviate symptoms associated with α-Syn pathology, necessitating studies of functional consequences of α-Syn aggregation.

Peritoneal carcinomatosis (PC), the spread of cancer cells in the peritoneum, is a significant concern in advanced gastrointestinal and gynecological cancers. This case series includes findings on the appearance and pattern of PC on 18F-fluorodeoxyglucose positron emission tomography/CT (18F-FDG PET/CT). The primary sources of peritoneal dissemination are direct invasion from abdominal or pelvic tumors and metastatic spread from distant tumors. The accurate preoperative diagnosis and quantification of PC play a vital role in determining the appropriate treatment approach, with a particular emphasis on surgical planning. Several imaging modalities have been employed in preoperative evaluation, such as computed tomography (CT), magnetic resonance imaging (MRI), and 18F-FDG PET/CT. Among these modalities, 18F-FDG PET/CT has demonstrated improved anatomical localization and accurate information about the nature of pathological findings. The case series showcases four cases that illustrate the imaging characteristics of PC on FDG PET/CT. FDG PET/CT plays a vital role in diagnosing and assessing PC, aiding in its detection, staging, and treatment planning. It surpasses conventional imaging techniques in identifying and characterizing lesions and detecting the primary tumor site in cases where its location is unknown. Furthermore, FDG PET/CT additionally assists in evaluating treatment response and monitoring disease progression, providing insights into treatment effectiveness and guiding patient management decisions.

The lightness of an object is an intrinsic property that depends on its surface reflectance spectrum. The visual system estimates an object's lightness from the light reflected off its surface. However, the reflected light also depends on object extrinsic properties of the scene, such as the light source. For stable perception, the visual system needs to discount the variations due to the object extrinsic properties. We characterize this perceptual stability for variation in two spectral properties of the scene: the reflectance spectra of background objects and the intensity of light sources. We measure human observers’ thresholds of discriminating computer-generated images of 3D scenes based on the lightness of a spherical target object in the scene. We measured change in discrimination thresholds as we varied the reflectance spectra of the objects and the intensity of the light sources in the scene, both individually and simultaneously. For small amounts of extrinsic variations, the discrimination thresholds remained nearly constant indicating that the thresholds were dominated by observers’ intrinsic representation of lightness. As extrinsic variation increased, it started affecting observers’ lightness judgment and the thresholds increased. We estimated that the effects of extrinsic variations were comparable to observers’ intrinsic variation in the representation of object lightness. Moreover, for simultaneous variation of these spectral properties, the increase in threshold squared compared to the no-variation condition was a linear sum of the corresponding increase in threshold squared for the individual properties, indicating that the variations from these independent sources combine linearly.

Purpose – The study aims to examine how the information disclosed by the managers in the management discussion and analysis (MD&A) reports varies at the different levels of corporate performance. Design/methodology/approach – To understand this quantile effect, first OLS technique was adopted and then, the quantile regression method was applied to explore the impact of MD&A disclosures on the firm performance across the lower and upper quantiles. The sample size for the study is 490 firms’ year observations for the period 2016–2022. Findings – The results of the study demonstrate the negative but significant relationship between MD&A disclosures and corporate performance, supporting the two management strategies of “competitive disadvantage” in case of good performance and “management impression strategy” in case of poor performance. Furthermore, with other corporate governance variables, both the size of the board and the number of independent directors on the board are positively significant only in the case of the upper quantile indicating the heterogeneity in the relationship between the performance and the MD&A disclosures. Therefore, the overall findings of the study support that these results contradict the agency theory and the stakeholders’ theory as managers are not acting well as agents on behalf of the investors and work well only when they are controlled by the large board having more independent directors. Originality/value – To the best of the authors’ knowledge, no study so far has incorporated quantile regression to assess the effect of MD&A disclosures on company performance at various levels of the firm performance, which gives more robust insights about the viewpoint of the managers on the different level of the firm performance. In other words, this study highlights the important information as to how the information provided in the MD&A reports varies as per the good or poor performance of the companies.

Abstract In the present study, three varieties of kidney beans (Red Rajma (RR), Rajma Chitra (RC), and Rajma Sharmili (RS)) were physically characterized and the effect of pre-gelatinization by microwave and hot water on functional and pasting properties of flour were studied. A significant difference was observed in the physical properties of all the kidney beans, indicating that they can be easily characterized and identified based on physical properties. Pre-gelatinization treatment led to variation in color, functional and pasting properties of flours. Color parameters L*, b*, chroma, and hue values reduced with the treatment, whereas a* and total color difference ( $$\Delta$$ Δ E) values increased. Water absorption capacity (WAC) and oil absorption capacity (OAC) varied in the range of 1.15–1.91% and 1.25–1.75% for microwave and hot water treatment, respectively. The highest value of WAC and OAC was observed for microwave-treated RC and hot water-treated RR, respectively. WAC increases with both treatments, whereas OAC increases only with water bath cooking. Functional group analysis (FTIR) showed no major peaks shifting in water bath treatment, whereas a slight deflation of certain peaks was observed in the case of microwave treatment. Pasting curves showed a significant reduction in peak and final viscosity after pre-gelatinization treatment; however, none of the samples showed any breakdown during cooking. Graphical Abstract

As Mohan (1986) rightly argues, While the need for coordinating the learning of language and subject matter is generally recognized, just how this should be accomplished remains a problem and is one of particular concern for university ESL/EFL programs. In view of this vital pedagogical concern, skills-integrated content courses have been designed and experimented by many universities and individual academicians. In content-based curriculum the basic organizational unit is a theme or topic, rather than the more customary grammatical patterns or language functions. The main goal of this, as Bycina (1982) explains, is to provide meaningful contexts for language learning instead of focusing on language as an object of study. At the foundation of this approach is the Krashen's (1984) notion that acquisition is best promoted when language is presented in comprehensible and interesting communicative contexts (p. 25). In a more crystalized view of English for STEM education, this paper revisits the concept of thinking and pedagogy of English for Specific Purposes (ESP) and emphasizes on the use of independent mind to promote focused ESP for the students of the Scientific Disciplines of Science, Technology, Engineering, and Mathematics (STEM). In the backdrop of the context of STEM education, we have developed a tripartite discussion in the paper focused on the primacy of independent mind and thinking skills; the need to facilitate language development in a contextualized, integrated, interactive framework; and the ways and means to exploit the richness of authentic scientific materials and discussion-led innovative tasks and activities to promote active ESP in STEM education.

As far as the main purpose of teaching and learning of the Grammar of a language is concerned, it should tell the teachers and learners the principles and parameters of sentence construction in the given language, i.e. English Language in the context of the discussion in this paper. Incidentally, the grammatical device of tense becomes more important and relevant at the level of discourse and communication. However, a predominantly common approach to teaching and learning of the system of tense in English language has been to understand it in synonymous terms with the notion of three timelines of present, past and future, which poses situations of systemic difficulties and makes it confusing and misleading to comprehend and communicate sentences and utterances in terms of communicative clarity within the parametric confines of the linguistic system of the English Language. Focusing on this issue, this paper demonstrates the ways to unfold the dichotomies involved in the traditional ways of teaching and learning of the grammar of tense, times and aspects of verbal action in English Language and suggests an instructional framework to resolve the related pedagogical issues of concern.

Like many non-native learners, English is a Foreign Language to Omani learners; therefore, Spoken English turns out to be difficult and often problematic. Non-native learners of English face several phonological problems mainly because most languages follow specific, predictable rules of pronunciation, but English does not. There are native and non-native varieties of English, which differ not only in terms of vocabulary and grammar but also in pronunciation. In the backdrop of phonological differences between varieties of English and ensuing pronunciation problems, this action research was conducted to investigate the phonological problems that Omani learners at school level encounter. The study was based on focused observation using the diary as a tool for data collection for six months each in one (1) class of grade seven (7) and one (1) class of grade nine (9) in two Omani public schools. The total sample size was 100. The collected phonological data were coded and organized in terms of marked thematic categories. The recurrent problems which surfaced in the Spoken English of Omani learners involved in this study were pure vowel substitution for diphthongs [/schwa near-close back rounded vowel/] and /ei/, replacement of /p/ by /b/ sound, insertion of the vowel sound /I/ while pluralizing the words, syllabification of initial and final consonant clusters, deletion of /s/ sound occurring as the final element from consonant clusters, the alternation between /d3/ and /g, /3/ and /d3/, /t[Esh - voiceless palato-alveolar fricative]/ and /[Esh - voiceless palato-alveolar fricative]/ sounds, replacement of /t/ by /t[character omitted]/ sound, lengthening of certain vowel sounds, pronouncing 'r' in all phonetic environments, irregularities in the use of weak forms, and not following the rules of aspiration. Based on the emerging phonological problems, certain remedial activities were planned and used with the students, which helped improve their phonological problems considerably. These remedial activities are proposed and recommended as measures of pedagogical intervention.

ABSTRACT Sepsis and chronic infections with Pseudomonas aeruginosa, a leading “ESKAPE” bacterial pathogen, are associated with increased morbidity and mortality and skeletal muscle atrophy. The actions of this pathogen on skeletal muscle remain poorly understood. In skeletal muscle, mitochondria serve as a crucial energy source, which may be perturbed by infection. Here, using the well-established backburn and infection model of murine P. aeruginosa infection, we deciphered the systemic impact of the quorum-sensing transcription factor MvfR (multiple virulence factor regulator) by interrogating, 5 days post-infection, its effect on mitochondrial-related functions in the gastrocnemius skeletal muscle and the outcome of the pharmacological inhibition of MvfR function and that of the mitochondrial-targeted peptide, Szeto-Schiller 31 (SS-31). Our findings show that the MvfR perturbs adenosine triphosphate generation, oxidative phosphorylation, and antioxidant response, elevates the production of reactive oxygen species, and promotes oxidative damage of mitochondrial DNA in the gastrocnemius muscle of infected mice. These impairments in mitochondrial-related functions were corroborated by the alteration of key mitochondrial proteins involved in electron transport, mitochondrial biogenesis, dynamics and quality control, and mitochondrial uncoupling. Pharmacological inhibition of MvfR using the potent anti-MvfR lead, D88, we developed, or the mitochondrial-targeted peptide SS-31 rescued the MvfR-mediated alterations observed in mice infected with the wild-type strain PA14. Our study provides insights into the actions of MvfR in orchestrating mitochondrial dysfunction in the skeletal murine muscle, and it presents novel therapeutic approaches for optimizing clinical outcomes in affected patients.IMPORTANCESkeletal muscle, pivotal for many functions in the human body, including breathing and protecting internal organs, contains abundant mitochondria essential for maintaining cellular homeostasis during infection. The effect of Pseudomonas aeruginosa (PA) infections on skeletal muscle remains poorly understood. Our study delves into the role of a central quorum-sensing transcription factor, multiple virulence factor regulator (MvfR), that controls the expression of multiple acute and chronic virulence functions that contribute to the pathogenicity of PA. The significance of our study lies in the role of MvfR in the metabolic perturbances linked to mitochondrial functions in skeletal muscle and the effectiveness of the novel MvfR inhibitor and the mitochondrial-targeted peptide SS-31 in alleviating the mitochondrial disturbances caused by PA in skeletal muscle. Inhibiting MvfR or interfering with its effects can be a potential therapeutic strategy to curb PA virulence.

Abstract Concerns about climate change and the reliance on fossil fuel reserves have motivated researchers to identify new renewable sources of energy. Biomass holds the potential to replace fossil‐derived products with biofuels and bio‐based chemicals. Plant‐derived lipids are promising sources of biofuels; however, the production of plant oil often leads to the release of massive amounts of carbon dioxide due to deforestation and land‐use change. The production of biofuels via plant oils (such as soybean) also competes with food production and in turn, impacts biodiversity. To mitigate these issues, the production of lipids from oleaginous yeasts could be an excellent alternative by incorporating these microbes into biorefineries utilizing agricultural or forest residues. Eventually, these microbial lipids could be potential sources for producing lipid‐derived biofuels. However, the current conventional methods for the production and recovery of lipids from oleaginous microbes suffer from economic and ecological challenges that affect its industrial‐scale expansion. This review highlights the major economic and environmental bottlenecks for the production of lipid‐derived biofuels from oleaginous yeasts. It also provides perspectives on the strategies that could be adapted on economic and ecological fronts to assist the expansion of the production of microbial lipid‐derived biofuels at an industrial scale.

The present study evaluated the effect of various pre-treatments (Potassium metabisulphite (KMS), KMS+Ultrasonication (KMSUS), KMS+Microwave (KMSMW), KMS+Hot water (KMSHWB) followed by infrared drying on the quality of Elephant Foot Yam (EFY). KMSMW-treated samples showed the fastest drying rate and highest moisture diffusivity, whereas the KMS-treated sample had the highest weight gain (171.57 %) in rehydration (80 °C). KMSMW and KMSHWB-treated samples exhibited softer textures in rehydration compared to control. Pre-treated samples had higher water absorption capacity and lower oil absorption capacity compared to the control. Pre-treatments also influenced the amylose content and the highest amylose content was observed for KMSUS (21.25 %). Pre-treatments positively influenced the retention of bioactive compounds and reduced the calcium oxalate (117.37 mg/g to 64.09 mg/g). Among all the pre-treatments, KMSHWB showed higher retention of bioactive compounds viz., TPC (297.67 mg GAE/100 g), TFC (922.22 mg QE/100 g), DPPH scavenging activity (57.91 %), ABTS (1.42 mmol TE/100 g) and lowest calcium oxalate (64.09 mg/100 g).

The present study focuses on the utilization of non-conventional starch (NS) derived from guinea grass seed to produce porous starch (PS) and octenyl succinic anhydride-modified PS (OSAPS). The study also introduces a novel approach to produce PS, aiming to overcome the high cost associated with glucoamylase. The formation of porous granules was successfully achieved through the combination of alpha-amylase and ultrasonication, as confirmed by electron micrographs and analysis of pore size and specific surface area. The results demonstrate that OSAPS exhibits superior emulsion stability, well-defined pore structures, and desirable amphiphilic characteristics. All three starches were loaded with lycopene, flaxseed oil, and a combination of both to prepare microcapsules. The presence of flaxseed oil within the starch matrix was confirmed through micrographs and functional group analysis. Notably, OSAPS exhibited the highest encapsulation efficiency at 74.80 %, along with a lycopene retention rate of up to 27.32 mg/100 g. Moreover, OSAPS showcased favorable antioxidant activity (89.29 %), phenolic content (0.45 mg GAE/g), and flavonoid content (97.61 mg QE/100 g). These findings underscore the remarkable potential of OSAPS as a delivery system for bioactive compounds, particularly for the co-encapsulation of oils and pigments.

The aim of this study is to examine the impact of corporate characteristics on voluntary disclosures of management discussion and analysis (MD&A) reports in India. Using a formal tone, the data was extracted from the annual reports of the top 100 listed firms available on the CMIE Prowess database for seven years (2016–2022). After excluding 23 companies from the financial and insurance sector, a panel regression method with the assistance of Gretl software was employed to investigate the relationship between the Management Discussion and Analysis Disclosure Index (MDADI) for voluntary aspects and various corporate attributes, with a total of 490 firm years of balanced observations. In India, firms follow the mandatory compliance of the MD&A reports, but voluntary disclosures are somehow those which are not much emphasized but are a good indication of firm performance and their accountability towards their stakeholders (Mayew et al., 2015). Our empirical findings reveal that profitability as a proxy to firm performance has a significant positive relationship with MD&A voluntary disclosures. Further, an insignificant association between VDS (Voluntary Disclosure Score) and the board size, presence of independent directors and firm size was found. This indicates that firm performance plays a significant role in adding more voluntary disclosures in MD&A reports. The possible reason for this could be the use of “Management Impression Strategy” in the MD&A reports, which means managers disclose more only when the firm has earned more and use impressive language to attract stakeholders. The outcomes of this research offer valuable insights for regulators, policymakers, and listed companies in India, aiding in the enhancement of MD&A reporting quality. Additionally, this study provides a roadmap for future research on MD&A reporting quality and corporate attributes in other emerging countries that have similar regulatory frameworks. This paper makes a timely and pertinent contribution to the scholarly discourse by shedding light on the relationship between MD&A disclosures and firm attributes. Its findings provide valuable insights for both academia and industry.

Aim: The aim and objective of this in vitro study was to evaluate the antibacterial efficacy of mineral trioxide aggregate, bioactive glass sealer, and epoxy-resin–based sealer. Materials and Methods: In the present study, 22 Mueller Hinton agar (MH agar) plates were employed and equally divided into two groups. Three holes were made by removal of agar at equidistant points and filled with root canal sealers. The strains of the bacteria used in this study were S. aureus (ATCC 6538) and C. albicans (ATCC 10231) and were divided into two groups and root canal sealers were divided into three subgroups: mineral trioxide aggregate (MTA) fillapex Sealer, Nishika Bioactive Glass sealer, and Syntex Epoxy Resin base sealer. For Staphylococcus aureus, peptone water was placed in a 2 mL test tube and bacteria were extracted from blood agar plates using a nichrome wire loop and poured into the peptone water–containing test tube and incubated for 2 hours and for C. albicans, fungi were grown at 37°C for 24 hours in MH Broth and seeded into MH agar to produce turbidity of 0.5 on the McFarland scale, which corresponds to a concentration of 108 CFU/mL. This MH broth was used as a second layer. The seeded agar was then added over the plates immediately after the insertion of sealer cement. After incubation, the diameters of zones of inhibition around the plates were measured. Results: The results of this study showed that the highest inhibition was recorded in Syntex sealer against Staphylococcus aureus followed by MTA fillapex sealer and Nishika sealer, whereas MTA fillapex showed the highest inhibition against C. albicans followed by Syntex sealer and Nishika sealer. Conclusion: Syntex sealer exhibits better antibacterial efficacy against Staphylococcus aureus and MTA fillapex exhibit better antibacterial efficacy against C. albicans.

Introduction: Seizure is the most common paediatric neurological condition with an increased predilection for early childhood. The recent definition of epilepsy includes first-onset seizures with an increased probability of future episodes. Thus, stringent evaluation of the initial episode of seizure is essential. Hence, this study estimated the aetiology, clinical profile, treatment and outcome of the first seizure episode in children at a tertiary hospital. Materials and Methods: This prospective observational study was carried out at a tertiary hospital in Barabanki, India. Children in the age group of 1 month to 12 years who presented with the first seizure episode and those admitted for other illnesses and developed the first seizure episode during hospital stay were included. Data collected were sociodemographics, clinical presentations, laboratory tests, brain imaging studies, electroencephalography (EEG), diagnosis, treatment and short-term outcomes. Results: A total of 113 patients were enrolled presenting with first-onset seizures. There were 78 (69%) males, 86 (76.1%) were below 5 years of age and 60 (53.1%) presented with fever. Most seizures had a generalised onset (58.4%). Febrile seizures (29.2%) were the most common type, followed by neurocysticercosis (20.3%). Central nervous system (CNS)-infective aetiology (58.7%) was the most common etiological classification. Abnormal findings were observed in cerebrospinal fluid examination of 24 out of 56, in brain imaging of 53 out of 80 and in EEG of 70 out of 113 cases examined. Anti-seizure medication (ASM) was prescribed to 68 patients, of which 10 had a recurrence in the 6-month follow-up period. Conclusion: The first episode of seizure in children usually had a generalised onset, with younger children of the male sex at an increased risk. CNS infections and febrile seizures were common causes. Complete history and neurological evaluation can guide the relevant investigations. ASM tailored to the aetiology controls the seizures in most cases.

Abstract Autosomal dominant pathogenic mutations in Leucine-rich repeat kinase 2 (LRRK2) cause Parkinson’s disease (PD). The most common mutation, G2019S-LRRK2, increases the kinase activity of LRRK2 causing hyper-phosphorylation of its substrates. One of these substrates, Rab10, is phosphorylated at a conserved Thr73 residue (pRab10), and is one of the most abundant LRRK2 Rab GTPases expressed in various tissues. The involvement of Rab10 in neurodegenerative disease, including both PD and Alzheimer’s disease makes pinpointing the cellular and subcellular localization of Rab10 and pRab10 in the brain an important step in understanding its functional role, and how post-translational modifications could impact function. To establish the specificity of antibodies to the phosphorylated form of Rab10 (pRab10), Rab10 specific antisense oligonucleotides were intraventricularly injected into the brains of mice. Further, Rab10 knock out induced neurons, differentiated from human induced pluripotent stem cells were used to test the pRab10 antibody specificity. To amplify the weak immunofluorescence signal of pRab10, tyramide signal amplification was utilized. Rab10 and pRab10 were expressed in the cortex, striatum and the substantia nigra pars compacta. Immunofluorescence for pRab10 was increased in G2019S-LRRK2 knockin mice. Neurons, astrocytes, microglia and oligodendrocytes all showed Rab10 and pRab10 expression. While Rab10 colocalized with endoplasmic reticulum, lysosome and trans-Golgi network markers, pRab10 did not localize to these organelles. However, pRab10, did overlap with markers of the presynaptic terminal in both mouse and human cortex, including α-synuclein. Results from this study suggest Rab10 and pRab10 are expressed in all brain areas and cell types tested in this study, but pRab10 is enriched at the presynaptic terminal. As Rab10 is a LRRK2 kinase substrate, increased kinase activity of G2019S-LRRK2 in PD may affect Rab10 mediated membrane trafficking at the presynaptic terminal in neurons in disease.

Abstract Background Oleaginous yeasts are a promising candidate for the sustainable conversion of lignocellulosic feedstocks into fuels and chemicals, but their growth on these substrates can be inhibited as a result of upstream pretreatment and enzymatic hydrolysis conditions. Previous studies indicate a high citrate buffer concentration during hydrolysis inhibits downstream cell growth and ethanol fermentation in Saccharomyces cerevisiae. In this study, an engineered Rhodosporidium toruloides strain with enhanced lipid accumulation was grown on sorghum hydrolysate with high and low citrate buffer concentrations. Results Both hydrolysis conditions resulted in similar sugar recovery rates and concentrations. No significant differences in cell growth, sugar utilization rates, or lipid production rates were observed between the two citrate buffer conditions during batch fermentation of R. toruloides. Under fed-batch growth on low-citrate hydrolysate a lipid titer of 16.7 g/L was obtained. Conclusions Citrate buffer was not found to inhibit growth or lipid production in this engineered R. toruloides strain, nor did reducing the citrate buffer concentration negatively affect sugar yields in the hydrolysate. As this process is scaled-up, $131 per ton of hydrothermally pretreated biomass can be saved by use of the lower citrate buffer concentration during enzymatic hydrolysis. Graphical Abstract

Since May 2022, mpox has been identified in 108 countries without endemic disease; most cases have been in gay, bisexual, or other men who have sex with men. To determine number of missed cases, we conducted 2 studies during June–September 2022: a prospective serologic survey detecting orthopoxvirus antibodies among men who have sex with men in San Francisco, California, and a retrospective monkeypox virus PCR testing of swab specimens submitted for other infectious disease testing among all patients across the United States. The serosurvey of 225 participants (median age 34 years) detected 18 (8.0%) who were orthopoxvirus IgG positive and 3 (1.3%) who were also orthopoxvirus IgM positive. The retrospective PCR study of 1,196 patients (median age 30 years; 54.8% male) detected 67 (5.6%) specimens positive for monkeypox virus. There are likely few undiagnosed cases of mpox in regions where sexual healthcare is accessible and patient and clinician awareness about mpox is increased.

Objectives: Following the COVID-19 pandemic, global epidemiological trends demonstrate a return to pre-pandemic levels of respiratory syncytial virus (RSV) and influenza (Flu) A/B viruses. For the appropriate clinical management of viral infections, reliable and timely diagnosis is crucial. The clinical presentation of these respiratory viral infections shows significant overlaps; thus, the syndromic diagnosis of these infections becomes challenging. The goal of this study was to compare the performance of three multiplex real-time PCR-based platforms for the detection of SARS-CoV-2, Flu A, Flu B, and RSV. Materials and Methods: A retrospective study was performed on 200 de-identified nasopharyngeal and oropharyngeal specimens. All samples were tested simultaneously on three PCR-based platforms for the detection of SARS-CoV-2, Flu A, Flu B, and RSV: HealthTrackRx’s real-time PCR Open Array® respiratory panel, TrueMark™ SARS-CoV-2, Flu A, Flu B, RSV Select Panel, and BioFire® RP2.1 Panel. The positive and negative predictive value of each test was evaluated at a 95% confidence interval. Results: Among the 200 tested samples, the TrueMark™ and OpenArray® laboratory-developed tests (LDTs) showed a 100% concordance for the detection of SARS-CoV-2, Flu A, Flu B, and RSV. Overall agreement of 100% was observed for nasopharyngeal samples between the laboratory-developed tests and FDA-approved BioFire® RP2.1 Panel. Diagnostic results for these four respiratory viruses, in clinical samples, between the LDTs and the FDA-approved comparator demonstrated full concordance. Conclusions: Respiratory viral infections represent one of the major global healthcare burdens. Consequently, the accurate detection and surveillance of these viruses are critical, particularly when these viruses are known to co-circulate. The excellent performance and full concordance of the LDTs, with the BioFire® Respiratory RP2.1 panel, in detecting SARS-CoV-2, Flu A, Flu B, and RSV shows that these tests can be confidently implemented for the clinical testing of respiratory viral infections.

Abstract Purpose: To present a rare case of metastatic conjunctival adenosquamous carcinoma (ASC) in the context of limited literature on the prognosis of ASC and suggested follow-up and surveillance. Case Report: We report a case of conjunctival ASC that metastasized to cervical lymph nodes five years after histological confirmation of complete local excision. Conclusion: Long-term clinical follow-up and surveillance imaging are warranted to allow early detection of disease recurrence and/or metastasis.

This paper attempts a pragmatic analysis of the interplay between social contexts of power and sociolinguistic device of aggravation strategies concerning dialogic discourses in Vikram Seth's novel "A Suitable Boy (ASB) (1993)." The paper attempts to validate that aggravation strategies have been an integral part of human discourse. It demonstrates how people use aggravation strategies to exercise power over others in different communicative contexts. It also exemplifies how power is vested in specific identities, and their role relationships in different power structures existing in the society based on their caste, age, sex, social standing, political or official identity, and how the power is exerted in the context of their social identities. The paper defines various aspects of aggravation, explains the dominant participatory identities, namely master identities, situated identities, and discourse identities and analyses how these social identities exercise power through aggravation strategies in the dialogic discourses in "ASB."

Strict outcome-bound approaches and text-books-based instructional practices, prevalent in the pedagogy of most of the colleges and universities in English as an International Language (EIL) contexts, involve language activities, tasks, and tests that predominantly require one right answer or response. Pedagogical practices and related quality assurance mechanisms regulated by such approaches limit students' ability to be original and skeptical in reflecting upon various issues of importance and concern based on their own thinking and experiences. Such a focus, in Sivasubramaniam's (2015) and Nunn and Sivasubramaniam's (2011) view, has entirely centered on bureaucratic efficiency aimed at having a uniform curriculum for the majority of the students and a scheme of research and evaluation based on recalls, think-alouds, cloze tests and multiplechoice questions in standardized texts. In line with the socially-aligned view of competence much needed spontaneity, flexibility, and diversity accrues only through a process-centered pedagogy of voice, agency and response. In the backdrop of this as a premise, this paper aims to demonstrate how Critical Thinking (CT) can be promoted in EIL classrooms as a discursive practice that could shape students' voice, agency and inter-subjectivity in a cohesive framework. The paper shares both theoretical and practical ideas about CT and its importance in facilitating a meaningful education and aims to demonstrate some innovative tasks and activities that could be exploited to shape student's voice and agency and develop their higher order CT skills. The paper culminates in evolving a practically viable prototype pedagogical framework for promoting CT as a social practice in EIL classrooms that is capable of making Wilga River's (1983) notions of 'skill-getting' and 'skill-using' a reality. Such a model will be useful for EIL practitioners in designing similar lessons with innovative tasks and activities and make the EIL class atmosphere stimulating and pedagogically more fruitful.