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Your search keyword '"Vieira Branquinho, Mariana"' showing total 4 results
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4 results on '"Vieira Branquinho, Mariana"'

2. Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: A comparative analysis on the umbilical cord and dental pulp derived Stem/ Stromal Cells secretome.

Author

Caseiro, Ana Rita, Santos Pedrosa, Sílvia, Ivanova, Galya, Vieira Branquinho, Mariana, Almeida, André, Faria, Fátima, Amorim, Irina, Pereira, Tiago, and Maurício, Ana Colette

Subjects
STROMAL cells, DENTAL pulp, UMBILICAL cord, NUCLEAR magnetic resonance spectroscopy, CHEMOTAXIS, CORD blood, HEPATOCYTE growth factor, VASCULAR endothelial cells
Abstract

Mesenchymal Stem/ Stromal Cells assume a supporting role to the intrinsic mechanisms of tissue regeneration, a feature mostly assigned to the contents of their secretome. A comparative study on the metabolomic and bioactive molecules/factors content of the secretome of Mesenchymal Stem/ Stromal Cells derived from two expanding sources: the umbilical cord stroma and the dental pulp is presented and discussed. The metabolic profile (Nuclear Magnetic Resonance Spectroscopy) evidenced some differences in the metabolite dynamics through the conditioning period, particularly on the glucose metabolism. Despite, overall similar profiles are suggested. More prominent differences are highlighted for the bioactive factors (Multiplexing Laser Bear Analysis), in which Follistatin, Growth Regulates Protein, Hepatocyte Growth Factor, Interleukin-8 and Monocyte Chemotactic Protein-1 dominate in Umbilical Cord Mesenchymal Stem/ Stromal Cells secretion, while in Dental Pulp Stem/ Stromal Cells the Vascular Endothelial Growth Factor-A and Follistatin are more evident. The distinct secretory cocktail did not result in significantly different effects on endothelial cell populations dynamics including proliferation, migration, tube formation capacity and in vivo angiogenesis, or in chemotaxis for both Mesenchymal Stem/ Stromal Cells populations. [ABSTRACT FROM AUTHOR]

Published
2019
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3. Biomaterials and Cellular Systems at the Forefront of Peripheral Nerve Regeneration

Author

Maurício, Ana Colette, Alvites, Rui Damásio, Vieira Branquinho, Mariana, Caseiro, Rita, Santos Pedrosa, Sílvia, Luís, Ana Lúcia, Geuna, Stefano, and Varejão, Artur

Subjects
Medical
Abstract

Peripheral nerve injuries remain a common clinical complication, and currently available therapies present significant limitations, often resulting in poor and suboptimal outcomes. Despite significant developments in microsurgical approaches in the last decades, no effective treatment options have been disclosed. Current research focuses on the optimization of such microsurgical techniques and on their combination with other pro-regenerative factors, such as mesenchymal stem cells or biomaterials. Mesenchymal stem cells present a remarkable capacity for bioactive molecule production that modulates inflammatory and regenerative processes, stimulating peripheral nerve regeneration. In parallel, efforts have been directed towards the development of biomaterial nerve guidance channels and nerve conduits. These biomaterials have been optimized in terms of biodegradability, ability to release bioactive factors, incorporation of cellular agents, and internal matrix architecture (to enable cellular migration and mimic native tissue morphology and to generate and bear specific electrical activity). The current literature review presents relevant advances in the development of mesenchymal stem cell and biomaterial-based therapeutic approaches aiming at the peripheral nerve tissue regeneration in diverse lesion scenarios, also exploring the advances achieved by our research group in this field in recent years.

Published
2019

4. Peripheral nerve injury and axonotmesis: State of the art and recent advances.

Author

Alvites, Rui, Rita Caseiro, Ana, Santos Pedrosa, Sílvia, Vieira Branquinho, Mariana, Ronchi, Giulia, Geuna, Stefano, Varejão, Artur S.P., and Colette Maurício, Ana

Subjects
*EARLY medical intervention, *PERIPHERAL nervous system, *SCIATIC nerve injuries, *CRUSH syndrome, *NERVOUS system regeneration, *ACCIDENTS, *ART
Abstract

Peripheral nerve lesions are frequent occurrences in both human and animal patients, leading to important physiological and labor complications that affect the quality of life of those who suffer the injury. More severe injuries are often associated with poor nerve regeneration and inadequate functional recovery, even with early medical and surgical interventions. Peripheral nerve crush lesions are frequent and, therefore, an experimental lesion paradigm widely used in researches involving nerve injury models and therapies for its resolution. In recent years, many studies have focused on innovative approaches to peripheral nerve treatment after crush injuries with more or less success. This review addresses the theme of peripheral nerve injury, with a special focus on the axonotmesis lesion, its etiology, pathophysiological mechanisms, methods of functional evaluation and regenerative processes, therapeutic options and corresponding recent advances. [ABSTRACT FROM AUTHOR]

Published
2018
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