Search

A 20-day old calf was presented to the Adnan Menderes University, Veterinary Hospital, Department of Internal Medicine and less than one month old calf in the field was reported by the Veterinary Surgeon at different times in 2017 from different farms to Umurlu district. On arrival the present one case presented fever, tachycardia, tachypnea, anormal bleeding symptoms [hemolacria (bloody tears), epistaxis, ear bleeding, prolonged bleeding time following injection and recurrent bleeding all over the body after minor trauma or spontaneously] and the others had an spontaneously bleeding on injection side. As being informed by the owner of both calves there was no change after the treatment performed by initial Veterinary Surgeon for two weeks. In an attempt to determine alterations that might be related to probable bovine neonatal pancytopenia (BNP), blood gas analysis, selected coagulation parameters were analyzed. Only in the first case, performed both rapid ELİSA test kit (IDEXX SNAP ® BVDV AntigenTest, United States) and PCR (Veterinary Research Control Instıtute, Bornova, İzmir) tested for bovine virus diarrhea virus antigen that were all negative. According to blood gase analysis of first case, Hct (+(130.2 mmol/L), K++(4.03 mmol/L), iCa (1.21 mmol/L), HCO3 (32.8 mmol/L), BE (+6.5 mmol) and coagulation parameter in both cases PT (>101 sec) and fibrinogen (> 113 mg/dL) as well as APTT (64.1 and 67.3 sec) were analyzed respectively. As a result of histopathological analysis and postmortem examination of first case, macroscopically petechial hemorrhage on the whole body, linear bleeding in jejenum and microscopically excudate with fibrin, neutrophil and blood in intestinal villus, tonsillar crypt, and pulmonary alveolus were obtained while other case could not be performed. As a result of clinical examination and laboratory results were suspected BNP and this is first reported case of hemolacria in a calf and associated coagulation variations in calves in Eagean Region of Turkey.

In the present multidisciplinary study, the researchers on a field trial performed a Social Network approach and a group of 9 calves (3 males/6 females) aged 10 to 16 weeks. A 24 hours digital camera was used for location registration (X-Y position) and interactions on each calf, both individually and collectively. Based on the simple methodology nearest neighbor matrix positive and negative associations were analyzed. The field study lasted 4 weeks and the maximum interaction day was chosen, when individual calf activity was high. To those of 9 calves solely no 2 was infected with giardiasis on the first week, whereas calves with no: 2, 7, and 8 were also infected on the second week. No: 4 and 6 were also infected with a total of 5 calves on the third week. This was followed by the final 6 calves in total infected. During the observation period, both by investigators and camera records, infected calf 2 was in close contact with no: 8 (at morning observation) and a slight degree of contact with no: 3 and 4 which were detected with giardiasis on the third and fourt weeks, respectively. Due to close contact with no: 8, infection was determined on the second week, earlier than others (except no: 7). Calves with no: 1 and 5 were never infected nor determined by PCR, fecal smear, or rapid diagnostic test kits throughout the study. In conclusion, the present authors suggested that social networking might be an important predisposing factor for giardiasis infection among calves.

In the present case report, the authors inspected the feasibility of transferring unenriched (however moistured and pre-biotic treated) skin microbiota communities between two heterologous hosts, namely heterologue skin microbiota transplantation (hSmT), [from apparently healthy donor to other relevant and diseased dogs, with scabies, separately]. Two cross-bred, client-owned dogs (belonging to the same owners) with a diagnosis of scabies and intense pruritus were enrolled. Nivea Clear Up Strips were attached in a total of 3 various apparently healthy integumentary tissue of another donor dog and allowed to dry for 10-12 minutes. This was followed by peeling off, all 3 strips were then transferred to diseased skin tissue (laterolateral area in 2 dogs with scabies, separately) which were also irrigated with isotonic and allowed to attach for at least 12-15 minutes. Finally, all 3 strips were removed. Both in case I and II, Vas pruritus scores were decreased significantly beginning on day 0 (initial hSmT day) to days 21. Vas pruritus score ranged between 8-10 and 7-9, in case I and II, respectively, prior to hSmT [days -14 to day 0]. Prior to hSmT all skin scrape positivity were deemed available on days -14 to 0, whereas after day 2 of hSmT, all 2 dogs gave negative skin scraping results till the end of the study. It should not be unwise to draw preliminary conclusion that transfer of unenriched skin microbiota from a healthy donor to the diseased dogs with scabies, should have helped hastening clinical and parasitological recovery by manipulation of cutaneous microenvironment, Bu vaka raporunda yazarlar, iki heterolog konakçı arasında (görünüşe göre sağlıklı donörden diğer uyuzlu hasta köpeklere ayrı ayrı) zenginleştirilmemiş (ancak nemlendirilen ve probiyotik uygulanan) deri mikrobiyota topluluklarının transferinin (hDmT) uygulanabilirliğini inceledi. Uyuz tanısı konulan ve yoğun kaşıntısı bulunan sahipli (aynı hasta sahibine ait olan), melez ırkı 2 köpek çalışmaya dahil edildi. Nivea t bölgesi burun bantları, başka donör bir köpeğin görünüşte sağlıklı toplamda 3 farklı integümenter dokusuna yapıştırıldı ve 10-12 dakika kurumaya bırakıldı. Akabinde bantlar alınarak her 3 bant izotonik ile irrige edilen hasta deri dokusuna (her iki uyuzlu köpekte ayrı ayrı laterolateral bölgelere) yapıştırıldı en az 12-15 dakika yapışmasına izin verildi. Sonunda 3 bantta uzaklaştırıldı. Vas prurtius skorları, hem vaka I hem de II'de, 0. günden (hDmT başlangıç günü) 21. güne kadar önemli ölçüde azaldı. hDmT’den önce [-14. –(0.) günler] Vas kaşıntı skoru vaka I’ de 8-10, vaka II’ de 7-9 arasında değişmekteydi. hDmT’den önce, deri kazıntıları -14 ila 0. günlerde pozitif iken, hDmT’nin 2. gününden sonra, her iki köpekte çalışmanın sonuna kadar negatif elde edildi. Zenginleştirilmemiş deri mikrobiyotasının sağlıklı bir donörden hasta uyuzlu köpeklere aktarılmasının, deri mikroekolojisinin manipülasyonu ile klinik ve parazitolojik iyileşmeyi hızlandırmış olması gerektiği gibi bir ön yargıya varmak yanlış olmayacaktır.

Present research was conducted at the University of Aydin Adnan Menderes, Faculty of Veterinary. In a total of 10 cats with pruritic dermatoses were allocated, classified [Feline atopic skin syndrome (FaSs)] and adapted to the retrospective case controlled study on the basis of Polycheck in vitro Allergy Test, clinical signs, dermatoscopic and cytological examination. No prior drug prescription, nor immunosuppressive treatment were evident, which was an inclusion criteria. Complete blood count, serum biochemistry, and endocrine panels were all deemed available on days 0 and 21 for all cats involved. The Feline Dermatitis Extent and Severity Index (FeDESI) and a 10 cm visual analog scale (VAS) were deemed available as relevant prognostic parameters. A three strain invoving soil-based probiotic used via rectal route significantly decreased FEDESI and VAS pruritus scores after 10 days of rectal use. Pre-treament day 0 FEDESI scores (median ± SD) (106.3 ± 41.38), were significantly higher than scores on day 10 (15.3 ± 10.53) switching the severity of the disease to mild status in all cases. Furthermore, day 0 VAS pruritus scores were 7.4 ± 1.78 (mean ± SD) (prior to treatment), whereas owner VAS pruritus score was decreased to 1.3 ± 1.06 (mean ± SD) significantly (p=0.005). It should not be unwise to draw preliminary conclusion that 3 chained soil-based Bacillus probiotic was capable of short term cure for FaSs. Bacillus probiotics with safety usage should be added to therapeutical armamentarium of FaSs.

WOS: 000460503100008, We aimed to examine the effect of hot-iron disbudding on serum thiol-disulphide homeostasis levels as a marker of oxidant stress in relationship with trauma in calves under sedation, local anaesthesia, and the non-steroidal anti-inflammatory drug ketoprofen. A total of 30 Holstein calves were enrolled in the study and allocated into three groups: disbudded following sedation with xylazine (n=10) (group I); disbudded following sedation (xylazine) and local anaesthesia with lidocaine (n=10) (group II); and disbudded after sedation (xylazine), local anaesthesia (lidocaine), and ketoprofen (n=10) (group Ill). Blood samples were withdrawn before (0. min) and 30, 60, 90, and 120 min after dehorning. Serum native thiols, total thiols, and disulphide levels were detected with a novel assay. Native thiol and total thiol levels were reduced in all groups without any significance during the study period. At the 90th min of the study, native thiol levels in group II were significantly lower than in groups I and Ill. There were no significant alterations in total thiol levels in both groups. Disulphide levels showed no significant changes in group, time, and group by time interactions, but at the 60m min, groups I and Ill had the lowest levels. Disulphite/native thiol, disulphite/total thiol, and native thiol/total thiol levels had significant group alterations in the 60th min. The reduction of native thiol and total thiol levels in all groups without significance might be related to the antioxidant activity of plasma; however, it is thought that the pain management procedures should be related to the sensitive oxidative balance by thiols.

In the present case series 2 different cats with diagnosis of hypo- versus hyper-thyroidism with distinct clinical signs were reported. The first case was a 3-month-old male domestic short-hair kitten was presented with a disproportionate dwarfism. Radiographic examination of the long bones revealed epiphyseal dysgenesis and delayed epiphyseal ossification. Congenital primary hypothyroidism was established by low serum total thyroxine. Initial treatment composed of levothyroxine. The second case was referred to the clinic with unresolved dermatological problems. On referral alopecia, extensive crusting and matting of the haircoat were evident. Hyperthyroidism and related military dermatitis was detected based on elevated serum total thyroxine and free T4 levels along with skin punch biopsy results. Therapeutical armamentarium composed of methimazole and hyperimmune milk factor. Early recognition, diagnosis and treatment intervention all resulted in clinical remission and relief of clinical signs accompanied by normal thyroidal function and laboratory results in both cats., Bu olgu sunumunda iki farklı kedide karşılaşılan hipotiroidizme karşı hipertiroidizm ilişkili farklı klinik bulgular sunuldu. İlk olgumuz 3 aylık yaşta, erkek, short-hair ırkı yavru kedi olup aşırı cücelik şikayeti ile kliniğimize getirilmiştir. Radyografik bulgularında uzun kemiklerde epifiziyal disgenezis ve epifiziyal kemikleşmede geçikme tespit edildi. Kongenital hipotiroidzm tanısı düşük total tiroksin seviyesiyle desteklendi ve levotiroksin ile sağaltıma başlanıldı. İkinci olgumuz ise sağaltıma ragmen düzelmeyen dermatolojik bulgular ile kliniğimize başvurdu. Klinik muayenede alopesi, yaygın kabuklanma ve kıl örtüsünde matlaşma görüldü. Deriden alınan punç biyopsi sonuçları, artan serum total tiroksin ve serbest T4 ve ilişkili miliyar dermatitis ile hipertiroidizm tanısı konuldu. Bu olgumuzda metimazol ve hiperimmun süt faktörü başlanıldı. Sonuç olarak her iki olgumuzda da erken tanı ve sağaltım ilişkili klinik bulguların giderilmesine ve laboratuvar sonuçlarına göre normal tiroid fonksiyonlarına kavuşmalarına yol açtı.

Canine Visseral Leishmanisis (CVL), Eski Dünya ülkelerinde Leishmania infantum'dan (L. infantum) kaynaklanan ölümcül, kroniksistemik bir hastalıktır. Enfeksiyonda köpekler ev sahibi ve rezervuar rolünüoynar. Bu bağlamda, bulaşmış köpekler hem insanlara hem de diğer köpeklereyönelik bir tehdittir. L. infantumile enfekte olan köpeklerde güncel literatürde diğer etkilenen organlara vesistemlere ek olarak kardiyak tutulum da bildirilmektedir. Bu çalışmada,bilgisayarlı elektrokardiyografi ile atriyal iletim süresinin ölçülmesiyoluylaPd'nin kullanımı ve bununla birlikte kardiyak troponin I (CTnI) seviyesindebelirlenen değişikliklerin, CVL'li köpeklerin evrelerine göre muhtemel kalphasarının klinik olarak değerlendirilmesi amaçlanmıştır.Hipertrikozis, perioküleralopesi, kilo kaybı, onikogripozis, lenfadenopati, hepatosplenomegali ve derilezyonları (şiddetli kabuklanma, alopesi ile uyumlu eksfoliatif dermatitis) ve/ veya anoreksiya gibi bir veya daha fazla klinik bulgunun bulunduğu her ikicinsiyetten ve çeşitli yaştan toplam 24 köpek, karşılaştırma için sağlıklıkontrol grubunda ve kardiyak hasarın varlığı, niteliği ve seviyesini belirlemekiçin CVL'li köpeklerde, bilgisayarlı 12 uçlu EKG cihazı [dinlenme ve 50 mm /sn'de 1 mV / cm amplitüd] ( Pd ölçümü)] ve serum CTnI konsantrasyonları, türeözgü ticari test kiti kullanılarak ölçüldü.Farklı klinik işaretiolan / olmayan CVL (IFAT pozitif) ile enfekte 18 köpek, üç farklı gruba (n = 6)alındı; yukarıda bahsedilen klinik belirtiden, tek klinik belirtiyle(oligosemptomatik) I grubuna dahil olanlar; iki veya çok sayıda klinikbulguları olan (polisemptomatik) II. grupta ve asemptomatik köpeklere III. grupdahil edildi. Herhangi bir hastalığı olmayan CVL negatif sağlıklı köpekler IV.gruba dahil edildi.CVL ile enfekte 18köpekten 10'unda tüm polimerik semptomları olan köpeklerle yüksek düzeyde cTnIkonsantrasyonu tespit edildi. Kontrol grubunun tüm vakalarında cTnI seviyelerireferans aralık [ 0.05). Pd değerlerinin ortalama ± standartsapması sırasıyla kontrol grubu, asemptomatik grup, oligosemptomatik grup vepolisemptomatik grupta 22.76 ± 3.12, 22.03 ± 0.80, 22.73 ± 0.80 ve 25.67 ± 1.41idi. Gruplar arasında karşılaştırıldığında, polisemptomatik grup kontrolgrubundan (p = 0.026), asemptomatik (p = 0.012) ve oligosemptomatik (p = 0.027)gruptan anlamlı farklılık gösterdi.Bu çalışmada CVL pozitifve kontrol olgular arasında istatistiksel olarak anlamlı fark bulunmasa da,bireysel artışın hastalığa bağlı olarak miyokardit ile ilişkiliolabileceğinigöstermnektedir. Ayrıca, özellikle polisemptomatik köpeklerdesaptanan ortalama Pd değerlerinin kontrol grubuna göre daha yüksek olduğu iddiaedilebilirken bu durum sağlıklı köpeklerde bildirilen ortalama Pd değerlerinedayalı referans aralıklarında kabul edilebilmekıtedir. Ancak, enfekteköpeklerin her bir grupta 6 adet olduğu düşünülürse, daha fazla sayıdakiolgunun araştırılmasının garanti altına alınabileceği iddia edilebilir., CanineVisceral Leishmanisis (CVL) is mostly fatal chronic systemic disease caused by Leishmaniainfantum (L. infantum) in theOld World countries. In infection, dogs plays both host and reservoir role. Bythis context, infected dogs are a threat to both people and other dogs. In dogsinfected with L. infantum, cardiacinvolvement in addition to the other affected organs and systems were alsoreported in the updated literatures. In the present study, the aim was toclinically evaluate the probable cardiac damage in dogs with CVL according toits stage via measuring atrial conduction time by use of Pd determined withincomputerized electrocardiography and cardiac troponin I (CTnI) level. Atotal of 24 dogs, of both sexes and various ages, referred with one or more ofthe clinical findings such as hypertrichosis, periocular alopecia, weight loss,onychogryphosis, skin lesions (severe scaling, exfoliative dermatitiscompatible with alopecia) and/or anorexia, lymphadenopathy, hepatosplenomegaly.In the healthy control group for comparison and in dogs with CVL to determinepresence, nature and level of cardiac damage, the evaluations were performedwith computerized 12-lead ECG device [(1 mV/cm amplitude in resting and 50mm/sec) (Pd measurement)] and serum CTnI concentrations were measured by usingspecies specific commercial test kit.Eighteendogs infected with CVL (IFAT positive) with/without different clinical signwere enrolled into three different groups (n=6); of the above mentionedclinical sign, to those of which with single clinical sign (oligosymptomatic)were included in the group I; those with multiple or several clinical signs(polysymptomatic) were included in the group II; and asymptomatic dogs wereincluded group III. CVL-negative healthy dogs without any disease were left ingroup IV.Highlevels of cTnI concentration were detected in 10 of 18 dogs infected CVL withall polysymptomatic dogs. In all cases of the control group, cTnI levels werein the reference range [0.05) was found between CVL positive andcontrol dogs. Mean ± standard deviationof Pd values were 22.76±3.12, 22.03±0.80, 22.73±0.80 and 25.67±1.41 in thecontrol group, asymptomatic group, oligosymptomatic group, and polysymptomaticgroup, respectively. In comparison between groups, polysymptomatic group wassignificantly different than control (p = 0.026), asymptomatic (p = 0.012) andoligosymptomatic (p = 0.027) groups. Althougha statistically significant difference was not found between CVL positive andcontrol dogs in the present study, it was suggested that the individualincrease may be associated with myocarditis due to disease. Besides, it may beclaimed that the mean Pd values determined in especially polysymptomatic dogswas higher compared to the control group, whereas this may be accepted in thereference ranges based on mean Pd values reported in healthy dogs. Howeverconsidering infected dogs population as 6 in each group, it may be safelyclaimed that further investigations regarding greater number of cases the maybe warranted.

WOS: 000444092000014, Objective. The present study was conducted to measure D-dimer concentrations and assess their value in disease activity in dogs with giardiasis. Furthermore another purpose was to analyze correlation between cyst excretion and D-dimer levels to those of dogs naturally infected with Giardia sp. Materials and methods. D-dimer analysis were performed in three groups of dogs; (i) 15 dogs with giardiasis to those of treated with secnidazol, (ii) 10 dogs with giardiasis, left untreated as control group, then were compared to those of (iii) 17dogs without giardiasis, used to detect reference ranges for D-dimer values as control group. was a correlation between D-dimer levels and logarythmic cyst counts. Results. The D-dimer range in healthy dogs was < 0.1 mg/L. In dogs with giardiasis, the D-dimer concentrations were greater than those of healthy dogs (p

Given developing data and knowledge everyday, science related novelty within the Veterinary InternalMedicine shine like a star. A star is born; immunostimulation via probiotherapy and/or fecal microbiotatransplantation. As an important component of the latter branch, Veterinary Dermatology has changedwithin the last 10 years and shifted to another West side of the story. Understanding gut-brain-skin axis,our mind has been shifted to determine gastroenterology and dermatology is somewhat like famoustwosomes such as ‘Blues brothers’ or ‘Metin Akpınar-Zeki Alasya’. The gut and skin, as an importantcomponents of gastroenterology and dermatology, highl vascularized and entirely innervated organs withneuroendocrine and immune participation, are aberrantly in association with purpose and function. Bothorgans are crucial for maintaining physiological homeostasis. Available data presented closely acquainted,bifacial relationship between the gut and skin. Apart from several research possessing a link forgastrointestinal health to skin homeostasis/allostasis. Gastrointestinal and dermatological disorders areinterlocked, oftenly allergy, histamin intolerance and gastrointestinal signs are accompanied by cutaneousmanifestations, in relation with the gut microbiome. In this presentation the gut microbiome’s contributionto gastrointestinal and skin disorders along with pathogenesis (i.e. gut-brain- skin axis) intestinal barrierfunctioning, leaky gut, disbiosis and unbalanced microbiota), analytic methodology (16 s rRna analysis,significance of phylum, class, order, family, genus detection to those of gut microbiota), , treatmentoptions will be discussed. Interestingly the participation of fecal microbiota transplantation and alteredcalendar probiotic treatment will be discussed in detail. The author (from his large archive) will bepresenting case series of atopic dermatitis along with pruritic dermatoses, which were succesfully treated.

We aimed to examine the effct of hot-iron disbudding on serum thiol-disulphide homeostasis levels as a marker of oxidant stress in relationship with trauma in calves under sedation, local anaesthesia, and the non-steroidal anti-inflmmatory drug ketoprofen. A total of 30 Holstein calves were enrolled in the study and allocated into three groups: disbudded following sedation with xylazine (n=10) (group I); disbudded following sedation (xylazine) and local anaesthesia with lidocaine (n=10) (group II); and disbudded after sedation (xylazine), local anaesthesia (lidocaine), and ketoprofen (n=10) (group III). Blood samples were withdrawn before (0. min) and 30, 60, 90, and 120 min after dehorning. Serum native thiols, total thiols, and disulphide levels were detected with a novel assay. Native thiol and total thiol levels were reduced in all groups without any significance during the study period. At the 90th min of the study, native thiol levels in group II were significantly lower than in groups I and III. There were no significant alterations in total thiol levels in both groups. Disulphide levels showed no significant changes in group, time, and group by time interactions, but at the 60th min, groups I and III had the lowest levels. Disulphite/ native thiol, disulphite/total thiol, and native thiol/total thiol levels had significant group alterations in the 60th min. The reduction of native thiol and total thiol levels in all groups without significance might be related to the antioxidant activity of plasma; however, it is thought that the pain management procedures should be related to the sensitive oxidative balance by thiols., Bu çalışmada sedasyon, lokal anestezi ve non-steroidal bir ilaç olan ketoprofen uygulanmış sıcak koterizasyon işlemi ile boynuzsuzlaştırılan buzağılarda oksidatif stresin değerlendirilmesinde tiyol-disülfüt homeostazına olan etkilerinin belirlenmesi amaçlandı. Çalışmaya toplam 30 adet Holstein buzağı dahil edilerek üç gruba ayrıldı: sedasyon (xylazine) işlemini takiben boynuzsuzlaştırılan (n=10) (grup I); sedasyon (xylazine) ve lidokain ile lokal anestezi işlemi sonrasında boynuzsuzlaştırılan (n=10) (grup II); ve sedasyon (xylazine), local anestezi (lidocaine) ve ketoprofen uygulaması sonrasında boynuzsuzlaştırılan (n=10) (group III). Kan örnekleri, boynuzsuzlaştırmadan önce (0. dak) ve 30, 60, 90 ve 120 dakika sonra olacak şekilde toplandı. Serum natif tiyol, total tiyol ve disülfür seviyeleri yeni bir test ile belirlendi. Çalışma süresince natif tiyol ve total tiyol seviyelerinde istatistiksel anlamlı olmayan azalmalar belirlendi. Araştırmanın 90. dakikasında grup II’de bulunan hayvanların natif tiyol seviyelerinin grup I ve III’e göre istatistiksel anlamlı düşüktü. Total tiyol seviyelerinde ise gruplar arasında istatistiksel anlamlı farklılıkların olmadığı belirlendi. Disülfit seviyelerinde grup, zaman ve grup zaman arasında farklılıklar belirlenmezken, araştırmanın 60. dakikasında grup I ve III’te en düşük seviyelere geldiği belirlendi. Disülfit/natif tiyol, disülfit/total tiyol ve natif tiyol/total tiyol seviyelerininde 60. dakikada gruplar arasında istatistiksel anlamlı değişimler gösterdiği belirlendi. Natif tiyol ve total tiyol seviyelerindeki bu azalmaların plazmadaki antioksidan kapasite ile ilişkili olabileceği, ancak uygulanan ağrı yönetimi prosedürlerinin tiyol ile oluşturulan hassas oksidatif dengeyi etkilediği düşünüldü.

Giardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals. Thepathogen is one of the most important causes of diarrhea in humans and farm animals. The aim of this study wasto determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis.The animal material of the study was used Sakız sheep which had naturally infected with Giardia duodenalisbetween 15-35 days of age from the Aydın region (n = 30), healthy lambs in the same age range (n = 15) and bothsexes. Sterile stool samples with the method of rectal touching of fecal samples from infected and healthyanimals, blood samples were obtained in a total of 5 ml of v.jugularis according to the technique in order todetermine serum 25-hydroxyvitamin D3 levels. Giardia screening was performed by direct microscopicexamination of stool samples. 25-OH-D levels were measured with Savant brand fluorescent immunoassay devicefrom the obtained serum samples. Mann-Whitney U test was used in statistical analysis. Statistically significantchanges were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p, Giardia duodenalis kuşlar, reptiller ve bir çok memeli hayvanı enfekte edebilen binükleuslu flagellalı bir protozoondur. Söz konusu patojen insanlarda ve çiftlik hayvanlarında başta olmak üzere şekillenen ishalin en önemli nedenleri arasında yer almaktadır. Çalışmada Giardia duodenalis ile doğal enfekte kuzularda serum 25- hidroksi vitamin D3 seviyelerindeki değişimlerin belirlenmesi amaçlanmıştır. Araştırmanın hayvan materyalini Aydın bölgesinde bulunan yaşları 15-35 günlük arasında her iki cinsiyetten sakız ırkı Giardia duodenalis ile doğal enfekte (n=30), benzer yaş aralığındaki aynı ırktan (n=15) ve her iki cinsiyetten sağlıklı kuzular oluşturdu. Enfekte ve sağlıklı hayvanlardan dışkı örnekleri rektal tuşe yöntemi ile steril dışkı örnekleri, serum 25-hidroksi vitamin D3 seviyelerinin belirlenmesi amacı ile de tekniğine uygun olarak v.jugularisden toplamda 5 ml olacak şekilde kan örnekleri alındı. Dışkı örneklerinden direkt mikroskobik bakı yöntemi ile giardia taraması yapıldı. Elde edilen serum örneklerinden Savant marka flöresan immunoassay cihazı ile 25-OH-D seviyeleri ölçüldü. İstatistiksel analizde Mann-Whitney U testinden yararlanıldı. Sağlıklı ve doğal enfekte hayvanlara ait 25-hidroksi vitamin D3 seviyeleri arasında istatistiksel (p

Objective. The present study was conducted to measure D-dimer concentrations and assess their value in disease activity in dogs with giardiasis. Furthermore another purpose was to analyze correlation between cyst excretion and D-dimer levels to those of dogs naturally infected with Giardia sp. Materials and methods. D-dimer analysis were performed in three groups of dogs; (i) 15 dogs with giardiasis to those of treated with secnidazol, (ii) 10 dogs with giardiasis, left untreated as control group, then were compared to those of (iii) 17dogs without giardiasis, used to detect reference ranges for D-dimer values as control group. was a correlation between D-dimer levels and logarythmic cyst counts. Results. The D-dimer range in healthy dogs was

In the present study the objective was to determine the effect of one transport journey on some stress indicators in horses. 11 adult stallion (all stallion) age 3-12 years including 7 Thoroughbred, 2 Arabian, 1 Dutch Warmblood, and 1 Friesian were enrolled. White blood cell counts and other relevant parameters were determined by an automated cell counter before and after transportation. Serum activities of relevant biomarkers were determined by use of an autoanalyzer. Serum cortisole level was detected by a microplate immunoassay technique. Sodium (Na), Potassium (K) and Clor (Cl) and Calsium (Ca) levels were also measured by use of autoanalyser. Mean Platelet (PLT), and Mean corpuscular hemoglobin concentration (MCHC) White Blood Cell (WBC) counts did not exhibit a variation for healthy horses during the study whereas RBC, Hb and Hct levels exhibited significant increases after transportation (P, Bu çalışmada, transportun atlarda bazı stres göstergelerine etkisini belirlemek amaçlanmıştır. 7 Safkan, 2 Arap, 1 Hollanda Yarım Kan ve 1 Fresyan olmak üzere 3-12 yaş arası 11 olgun at (aygır) kayıt edildi. Transport öncesi ve sonrasında, otomatik kan sayımı ile beyaz kan hücresi sayımı ve diğer ilgili parametreler belirlendi. İlgili biyolojik belirteçlerin serum aktiviteleri bir otoanalizör kullanılarak belirlendi. Na, K, Cl ve Ca düzeyleri de otoanalizör kullanılarak ölçüldü. Ortalama Platelet (PLT) ve Oratalama Hemoglobin Konsantrasyonu (MCHC) Total Lökosit (WBC) sayıları çalışma süresince sağlıklı atlar için bir farklılık göstermezken, Alyuvarlar (RBC), Hemoglobin (Hb) ve Hematokrit (Hct) düzeyleri ulaşımdan sonra anlamlı artışlar sergiledi (P