8 results on '"Ueda, Haruna"'
Search Results
2. Problem for Diet Management Ability Formation of High School Students
- Author
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Ueda, Haruna, Tatano, Michiko, and Tochigi, Mayumi
- Subjects
朝食の摂取状況 ,食生活管理能力 ,高校生の食生活実態 ,栄養バランス - Abstract
本研究は、家庭科において自らの食生活を営む力である食生活管理能力を形成するために、より効果的な学習内容や学習方法を検討することを目的とし、A県の高等学校1校1年生の5学級計167人を対象に、食生活の実態と意識についてのアンケート調査を行った。その結果、朝食を毎日取っているのは約3/4の者であり、朝食メニューは、「主食のみ」という者が男女ともに最も多かった。そして、メニューの栄養バランスを生徒自身が自己評価した結果、「わからない」と回答した者が多くを占めた。また、生徒の自己評価と調査者による評価とが一致した者は非常に少なく、自分の食事の栄養バランスを正確に判断する能力が身についている者は少ないことが明らかとなった。, さらに、朝食メニューの組み合わせ方と昼食の栄養バランスの関連性を見ると、朝食メニューの組み合わせ方のバランスがよい者は、昼食の栄養バランスがよい傾向にあり、食事についての基本的な考え方が影響しているものと考えられる。, また、夜食を取る頻度が高いことや食生活に関する家事参加の低いことが明らかとなった。それゆえ、自分1人で料理を作ることができるという者は約2/3であり、カレーライスやチャーハンのように一品料理を挙げている者が多かった。このように、食生活の実態と意識を明らかにした結果、生徒自身が自らの食生活を振り返る機会が少ないこと、自らの食生活の実態を踏まえての学習があまり行われていないことなどから生じたものと考えられる。したがって、まず学習前に自分の食生活を振り返り、その結果を活かして学習できるような学習資料の作成が必要であると思われる。これらの学習資料の活用により、自らがより良い食生活を送るための食生活管理能力の形成へとつなげていくことが、さらに今後の課題となる。
- Published
- 2016
3. The Reality and Problems of the Breakfast of the Freshman : Who Uses a University Co-op Cafeteria
- Author
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Tatano, Michiko, Fujihara, Saki, and Ueda, Haruna
- Subjects
大学生協食堂 ,朝食の実態 ,食生活教育 ,大学1年生 - Abstract
大学入学直後の1年生を対象にして、大学の生協食堂でどのような朝食メニューを取っているのかを、撮影した写真を分析することによって明らかにした。併せて食生活に関する実態調査を行い、食生活管理能力を育成するための課題を明らかにし、高校までの家庭科において食生活管理能力を育成するための基礎資料とすることを目的とした。, 今回の調査において、朝食を取っていない者はわずか数パーセントであった。そして、その朝食メニューの組み合わせは「主食+主菜+副菜+汁物・デザート」や「主食+主菜+副菜+汁物」というような、栄養や食品のバランスがとれているものは、約半数のものであった。残りの半数の者のメニューは、組み合わせのいずれかが欠けていたり、中には、「主食・汁物」のみというものもあったりで、量と栄養ともに不足していた。これらのニューを選んだ理由をみると、「好み」や「気分」が優先され、「栄養バランス」や「カロリー」などを考慮し、メニューを選択する者は少ないという結果であった。自分の取った朝食メニューの栄養バランスの自己評価と調査者評価との関連は低く、また評価そのものが出来ない者が約10%あった。これらのことから食生活の自己管理能力が備わっているとは言い難い状況にあることが理解できた。, 食生活に関する知識・調理技術の情報源は、男女とも約80%の者が「学校の家庭科」、約66%が「家庭」としており、学習したことを生活に活かすということから両者の連携が一層求められる。食生活に関する知識や調理技術の習得についての関心・意欲は高かった。, 今後の課題として、家庭科の学習において、実際の生活に結び付けて考えることのできる指導の工夫や、高等学校以降の生活を考慮した学習展開を工夫し、実践力が身に付くような指導を工夫し、食生活管理能力の育成を行う必要があると言える。
- Published
- 2016
4. Amino- and carboxyl-terminal ends of the bovine parainfluenza virus type 3 matrix protein are important for virion and virus-like particle release.
- Author
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Ueda, Haruna, Yamakawa, Nagisa, and Takeuchi, Kaoru
- Subjects
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VIRUS-like particles , *PARAINFLUENZA viruses , *MEMBRANE transport proteins , *VIRION , *BOS - Abstract
Paramyxovirus matrix (M) proteins are key drivers of virus particle assembly and budding at the plasma membrane. To identify regions important for the M protein function, we generated a series of deletion mutants of the bovine parainfluenza virus type 3 (BPIV3) M protein. We found that M proteins lacking 10 amino acids in the amino-terminal end (ΔN10) or 4 amino acids in the carboxyl-terminal end (ΔC4) did not support M-deficient BPIV3 virion release and M protein-induced virus-like particle (VLP) release. Both ΔN10 and ΔC4 retained M protein-M protein and M protein-nucleocapsid (N) protein interactions. However, neither was transported to the plasma membrane. Our results indicate that both amino- and carboxyl-terminal ends of the BPIV3 M protein are essential for M protein transport to the plasma membrane, where it facilitates virion and VLP release. [Display omitted] • M protein terminal ends are important for virion and VLP release. • M proteins without terminal ends were not transported. • The M protein YLDV sequence is critical for virion and VLP release. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
5. Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector.
- Author
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Sugasawa, Takehito, Nakano, Takuro, Fujita, Shin-ichiro, Matsumoto, Yuki, Ishihara, Genki, Aoki, Kai, Yanazawa, Koki, Ono, Seiko, Tamai, Shinsuke, Manevich, Lev, Ueda, Haruna, Ishibashi, Noriyo, Tamai, Kenshirou, Kanki, Yasuharu, Yoshida, Yasuko, Watanabe, Koichi, Takemasa, Tohru, Kawakami, Yasushi, and Takekoshi, Kazuhiro
- Subjects
LABORATORY mice ,GENETIC transformation ,HUMAN genes ,BLOOD cell count ,ANIMAL disease models - Abstract
Despite the World Anti-Doping Agency (WADA) ban on gene doping in the context of advancements in gene therapy, the risk of EPO gene-based doping among athletes is still present. To address this and similar risks, gene-doping tests are being developed in doping control laboratories worldwide. In this regard, the present study was performed with two objectives: to develop a robust gene-doping mouse model with the human EPO gene (hEPO) transferred using recombinant adenovirus (rAdV) as a vector and to develop a detection method to identify gene doping by using this model. The rAdV including the hEPO gene was injected intravenously to transfer the gene to the liver. After injection, the mice showed significantly increased whole-blood red blood cell counts and increased expression of hematopoietic marker genes in the spleen, indicating successful development of the gene-doping model. Next, direct and potentially indirect proof of gene doping were evaluated in whole-blood DNA and RNA by using a quantitative PCR assay and RNA sequencing. Proof of doping could be detected in DNA and RNA samples from one drop of whole blood for approximately a month; furthermore, the overall RNA expression profiles showed significant changes, allowing advanced detection of hEPO gene doping. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
6. Influence of Intermittent Cold Stimulations on CREB and Its Targeting Genes in Muscle: Investigations into Molecular Mechanisms of Local Cryotherapy.
- Author
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Sugasawa, Takehito, Tome, Yoshiya, Takeuchi, Yoshinori, Yoshida, Yasuko, Yahagi, Naoya, Sharma, Rahul, Aita, Yuichi, Ueda, Haruna, Maruyama, Reina, Takeuchi, Kaoru, Morita, Shohei, Kawamai, Yasushi, and Takekoshi, Kazuhiro
- Subjects
TIBIALIS anterior ,PHYSIOLOGICAL effects of cold temperatures ,COLD therapy ,SKELETAL muscle injuries ,GENE targeting ,CYCLIC adenylic acid ,MITOCHONDRIAL DNA - Abstract
Local cryotherapy is widely used as a treatment for sports-related skeletal muscle injuries. The molecular mechanisms are unknown. To clarify these mechanisms, we applied one to three 15-min cold stimulations at 4 °C to various cell lines (in vitro), the tibialis anterior (TA) muscle (ex vivo), and mouse limbs (in vivo). In the in vitro assay, cyclic AMP (cAMP) response element binding protein 1 (CREB1) was markedly phosphorylated (p-CREB1), and the CREB-binding protein (CBP) was recruited to p-CREB-1 in response to two or three cold stimulations. In a reporter assay with the cAMP-responsive element, the signals significantly increased after two to three cold stimulations at 4 °C. In the ex vivo study, CREB-targeting genes were significantly upregulated following two or three cold stimulations. The in vivo experiment disclosed that cold stimulation of a mouse limb for 9 days significantly increased mitochondrial DNA copy number and upregulated genes involved in mitochondrial biogenesis. The results suggest that local cryotherapy increases CREB transcription and upregulates CREB-targeting genes, in a manner dependent on cold stimulation frequency and duration. This information will inform further investigations into local cryotherapy as a treatment for sports-related skeletal muscle trauma. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
7. Detection of Transgenes in Gene Delivery Model Mice by Adenoviral Vector Using ddPCR.
- Author
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Sugasawa, Takehito, Aoki, Kai, Watanabe, Koichi, Yanazawa, Koki, Natsume, Tohru, Takemasa, Tohru, Yamaguchi, Kaori, Takeuchi, Yoshinori, Aita, Yuichi, Yahagi, Naoya, Yoshida, Yasuko, Tokinoya, Katsuyuki, Sekine, Nanami, Takeuchi, Kaoru, Ueda, Haruna, Kawakami, Yasushi, Shimizu, Satoshi, and Takekoshi, Kazuhiro
- Subjects
TRANSGENES ,GENETIC engineering ,GENES ,BLOOD cells ,GENE therapy ,INTRAVENOUS injections - Abstract
With the rapid progress of genetic engineering and gene therapy, the World Anti-Doping Agency has been alerted to gene doping and prohibited its use in sports. However, there is no standard method available yet for the detection of transgenes delivered by recombinant adenoviral (rAdV) vectors. Here, we aim to develop a detection method for transgenes delivered by rAdV vectors in a mouse model that mimics gene doping. These rAdV vectors containing the mCherry gene was delivered in mice through intravenous injection or local muscular injection. After five days, stool and whole blood samples were collected, and total DNA was extracted. As additional experiments, whole blood was also collected from the mouse tail tip until 15 days from injection of the rAdv vector. Transgene fragments from different DNA samples were analyzed using semi-quantitative PCR (sqPCR), quantitative PCR (qPCR), and droplet digital PCR (ddPCR). In the results, transgene fragments could be directly detected from blood cell fraction DNA, plasma cell-free DNA, and stool DNA by qPCR and ddPCR, depending on specimen type and injection methods. We observed that a combination of blood cell fraction DNA and ddPCR was more sensitive than other combinations used in this model. These results could accelerate the development of detection methods for gene doping. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
8. The detection of trans gene fragments of hEPO in gene doping model mice by Taqman qPCR assay.
- Author
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Aoki K, Sugasawa T, Yanazawa K, Watanabe K, Takemasa T, Takeuchi Y, Aita Y, Yahagi N, Yoshida Y, Kuji T, Sekine N, Takeuchi K, Ueda H, Kawakami Y, and Takekoshi K
- Abstract
Background: With the rapid progress of genetic engineering and gene therapy methods, the World Anti-Doping Agency has raised concerns regarding gene doping, which is prohibited in sports. However, there is no standard method available for detecting transgenes delivered by injection of naked plasmids. Here, we developed a detection method for detecting transgenes delivered by injection of naked plasmids in a mouse model that mimics gene doping., Methods: Whole blood from the tail tip and one piece of stool were used as pre-samples of injection. Next, a plasmid vector containing the human erythropoietin (hEPO) gene was injected into mice through intravenous (IV), intraperitoneal (IP), or local muscular (IM) injection. At 1, 2, 3, 6, 12, 24, and 48 h after injection, approximately 50 µL whole blood was collected from the tail tip. One piece of stool was collected at 6, 12, 24, and 48 h. From each sample, total DNA was extracted and transgene fragments were analyzed by Taqman quantitative PCR (qPCR) and SYBR green qPCR., Results: In whole blood DNA samples evaluated by Taqman qPCR, the transgene fragments were detected at all time points in the IP sample and at 1, 2, 3, 6, and 12 h in the IV and IM samples. In the stool-DNA samples, the transgene fragments were detected at 6, 12, 24, and 48 h in the IV and IM samples by Taqman qPCR. In the analysis by SYBR green qPCR, the transgene fragments were detected at some time point in both specimens; however, many non-specific amplicons were detected., Conclusions: These results indicate that transgene fragments evaluated after each injection method of naked plasmids were detected in whole-blood and stool DNA samples. These findings may facilitate the development of methods for detecting gene doping., Competing Interests: The authors declare there are no competing interests., (©2020 Aoki et al.)
- Published
- 2020
- Full Text
- View/download PDF
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