19 results on '"Tuomisto, Sari"'
Search Results
2. Thrombus Aspirates From Patients With Acute Ischemic Stroke Are Infiltrated by Viridans Streptococci.
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Patrakka, Olli, Tuomisto, Sari, Pienimäki, Juha-Pekka, Ollikainen, Jyrki, Oksala, Niku, Lampinen, Vili, Ojanen, Markus J. T., Huhtala, Heini, Hytönen, Vesa P., Lehtimäki, Terho, Martiskainen, Mika, and Karhunen, Pekka J.
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- 2023
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3. Urine specimen collection following consensual intercourse – A forensic evidence collection method for Y-DNA and spermatozoa
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Joki-Erkkilä, Minna, Tuomisto, Sari, Seppänen, Mervi, Huhtala, Heini, Ahola, Arja, and Karhunen, Pekka J.
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- 2016
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4. Clinical forensic sample collection techniques following consensual intercourse in volunteers – Cervical canal brush compared to conventional swabs
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Joki-Erkkilä, Minna, Tuomisto, Sari, Seppänen, Mervi, Huhtala, Heini, Ahola, Arja, Rainio, Juha, and Karhunen, Pekka J.
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- 2014
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5. Polymorphisms of PAI-1 and platelet GP Ia may associate with impairment of renal function and thrombocytopenia in Puumala hantavirus infection
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Laine, Outi, Joutsi-Korhonen, Lotta, Mäkelä, Satu, Mikkelsson, Jussi, Pessi, Tanja, Tuomisto, Sari, Huhtala, Heini, Libraty, Daniel, Vaheri, Antti, Karhunen, Pekka, and Mustonen, Jukka
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- 2012
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6. Evaluation of Postmortem Bacterial Migration Using Culturing and Real-Time Quantitative PCR*
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Tuomisto, Sari, Karhunen, Pekka J., Vuento, Risto, Aittoniemi, Janne, and Pessi, Tanja
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- 2013
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7. Age-dependent association of gut bacteria with coronary atherosclerosis: Tampere Sudden Death Study
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Tuomisto, Sari, Huhtala, Heini, Martiskainen, Mika, Goebeler, Sirkka, Lehtimäki, Terho, Karhunen, Pekka J., Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology, Yhteiskuntatieteiden tiedekunta - Faculty of Social Sciences, and Tampere University
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Adult ,DNA, Bacterial ,Male ,Science ,Cardiology ,Coronary Artery Disease ,Pathology and Laboratory Medicine ,Severity of Illness Index ,Microbiology ,Vascular Medicine ,Death, Sudden ,Feces ,Enterobacteriaceae ,Medicine and Health Sciences ,Bacteroides ,Humans ,Coronary Heart Disease ,Microbial Pathogens ,Aged ,Aged, 80 and over ,Clostridium ,Clostridiales ,Bacteria ,Gut Bacteria ,Sisätaudit - Internal medicine ,Age Factors ,Organisms ,Streptococcus ,Biology and Life Sciences ,Middle Aged ,Atherosclerosis ,Plaque, Atherosclerotic ,Bacterial Typing Techniques ,Gastrointestinal Microbiome ,Bacterial Pathogens ,Gastrointestinal Tract ,Lactobacillus ,Medical Microbiology ,Bacterial Translocation ,Case-Control Studies ,Medicine ,Bifidobacterium ,Pathogens ,Anatomy ,Digestive System ,Research Article - Abstract
BackgroundThe gut microbiome is thought to remain stable into old age. Gut bacteria and their translocation may play a role in the development of coronary heart disease (CHD) by modulating cholesterol levels and immune responses, as well as by producing toxic metabolites and bacterial endotoxins. The association of changes in the gut microbiome with the severity of coronary atherosclerosis and the ability of gut bacteria themselves to translocate into coronary plaques has not been studied.Materials and methodsAs a part of the Tampere Sudden Death Study, we measured age-dependent changes in the relative ratios of major intestinal bacterial communities (Bacteroides species [spp.], the Clostridium leptum group, the Clostridium coccoides group, Bifidobacterium spp., Enterobactericeae, Lactobacillus spp.) and Streptococcus spp. in both feces and coronary plaques of the same male autopsy cases (n = 67, age range 44-95) using real-time quantitative PCR (qPCR). The area of coronary atherosclerotic lesions were measured by computer-assisted morphometry. Fecal bacterial DNA measurements from healthy volunteers served as a control for gut bacterial analyses of autopsy cases. The relative amount of bacterial DNA in a sample was determined with the comparative Cq method.ResultsThe relative ratios of fecal Lactobacillus spp., Bifidobacterium spp., the Clostridium coccoides group, and Bacteroides spp. did not differ between controls and autopsy cases and showed no age-dependence. In contrast, the ratios of the Clostridium leptum group, Enterobactericeae, and Streptococcus spp. increased with age. Elevated relative ratios of fecal Enterobactericeae associated with a larger coronary plaque fibrotic area (p = 0.001), and the Clostridium leptum group with a larger calcification area (p = 0.015). Intestinal bacterial DNA could be amplified in 67.6% of the coronary plaques, the most common being Streptococcus spp. (41.0%), followed by Enterobactericeae (12.1%), Clostridium leptum (2.4%), and Lactobacillus spp. (2.4%). The percentages of Streptococcus spp. DNA decreased, and those of Enterobactericeae increased in coronary plaques along with age.ConclusionsDNA of the Clostridium leptum group and pathogenic Enterobactericeae increase in the gut microbiome with age and can be detected in the same individual's coronary plaques along with pathogenic Streptococcus spp., associating with more severe coronary atherosclerosis.
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- 2019
8. Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke.
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Patrakka, Olli, Mehtonen, Helena, Tuomisto, Sari, Pienimäki, Juha-Pekka, Ollikainen, Jyrki, Huhtala, Heini, Pessi, Tanja, Oksala, Niku, Lehtimäki, Terho, Järnstedt, Jorma, Martiskainen, Mika, and Karhunen, Pekka J.
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DNA analysis ,THROMBOSIS risk factors ,TEETH ,ISCHEMIC stroke ,CAROTID artery stenosis ,ORAL health ,CAROTID endarterectomy ,PERIPHERAL vascular diseases ,GRAM-negative bacteria ,PERIODONTAL disease ,STREPTOCOCCUS ,STROKE patients ,DESCRIPTIVE statistics ,COMPUTED tomography ,POLYMERASE chain reaction ,ODDS ratio ,MOUTH ,BACTERIA ,DISEASE risk factors ,DISEASE complications - Abstract
Background and purpose. Risk of acute ischemic stroke has been associated with carotid artery atherosclerosis as well as with periodontal disease. We studied whether oral pathology or carotid atherosclerosis was associated with the presence and quantity of bacterial DNA in their aspirated thrombi. Methods. Thrombus aspirates and control arterial blood were taken from 71 patients (70.4% male; mean age, 67.4 years) with acute ischemic stroke. Tooth pathology was registered using CT scans. Carotid stenosis was estimated with CTA and ultrasonography. The presence of bacterial DNA from aspirated thrombi was determined using quantitative PCR. We also analyzed the presence of these bacterial DNAs in carotid endarterectomies from patients with peripheral arterial disease. Results. Bacterial DNA was found in 59 (83.1%) of the thrombus aspirates (median, 8.6-fold). Oral streptococcal DNA was found in 56 (78.9%) of the thrombus aspirates (median, 5.1-fold). DNA from A. actinomycetemcomitans and P. gingivalis was not found. Most patients suffered from poor oral health and had in median 19.0 teeth left. Paradoxically, patients with better oral health had more oral streptococcal DNA in their thrombus than the group with the worst pathology (p = 0.028). There was a trend (OR 7.122; p = 0.083) in the association of ≥50% carotid artery stenosis with more severe dental pathology. Oral streptococcal DNA was detected in 2/6 of carotid endarterectomies. Conclusions. Stroke patients had poor oral health which tended to associate with their carotid artery stenosis. Although oral streptococcal DNA was found in thrombus aspirates and carotid endarterectomy samples, the amount of oral streptococcal DNA in thrombus aspirates was the lowest among those with the most severe oral pathology. These results suggest that the association between poor oral health and acute ischemic stroke is linked to carotid artery atherosclerosis. [ABSTRACT FROM AUTHOR]
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- 2021
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9. Lactase non-persistent genotype influences milk consumption and gastrointestinal symptoms in Northern Russians
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Khabarova Yulia, Tornianen Suvi, Tuomisto Sari, Järvelä Irma, Karhunen Pekka, Isokoski Mauri, and Mattila Kari
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract Background Milk is an important source of nutrients. The consumption of milk, however, may cause abdominal complaints in lactose intolerant individuals. The frequency of -13910C/C genotype is known to be high among Northern Russians, exceeding the prevalence in northern Europe. In our study we tested two hypotheses: 1) subjects with lactase non-persistent genotype (-13910C/C) have more gastrointestinal (GI) symptoms associated with milk 2) subjects with lactase non-persistence avoid using milk. Methods In total, 518 students aged 17 to 26 years were randomly selected from different departments in the Northern State Medical University (NSMU) for genotyping the lactase activity-defining -13910C/T variant. All subjects filled in a questionnaire covering their personal data, self-reported GI symptoms and milk consumption habits. Results Northern Russians consume very small amounts of milk daily. Among carriers of the lactase non-persistent (LNP) genotype there were 10 percentage units of milk-consumers fewer than among lactase-persistent (LP) subjects (p = 0.03). Complaints of GI disorders caused by milk were different between the genotypes (p = 0.02). Among all types of food analyzed only milk was associated with increased GI symptoms among subjects with the LNP genotype (OR = 1.95, CI 1.03-3.69) Conclusions Subjects with -13910C/C have more GI symptoms from milk. Subjects with lactase non-persistent genotype avoid using milk. In the case of increasing milk consumption symptoms may increase the need for medical consultation. It is thus important either for people themselves or for health care staff to be aware of lactase persistence/non-persistence.
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- 2011
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10. Time-dependent post mortem changes in the composition of intestinal bacteria using real-time quantitative PCR
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Tuomisto, Sari, Karhunen, Pekka J, and Pessi, Tanja
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Microbiota (Symbiotic organisms) -- Research -- Physiological aspects -- Methods ,Host-bacteria relationships -- Methods -- Physiological aspects -- Research ,Forensic sciences -- Research -- Physiological aspects -- Methods ,Polymerase chain reaction -- Methods -- Physiological aspects -- Research ,Postmortem changes -- Research - Abstract
Post mortem or even normal changes during life occurring in major gut bacterial populations are not known. We investigated Bacteroides sp., Bifidobacterium sp., Clostridium leptum, Clostridium coccoides, Streptococcus sp., Lactobacillus sp. and Enterobacteriacaea ratios in 7 fecal samples from healthy volunteers and in 61 autopsies rectum and cecum samples and studied the effect of post mortem time using quantitative real-time PCR. Bacterial ratios in stool samples from volunteers and rectum samples from autopsy cases were similar and did not change significantly up to 5 days post mortem. In cecum, significant post mortem time-dependent differences were observed in ratios of Bacteroides sp. (p = 0.014) and Lactobacillus sp. (p = 0.024). Our results showed that ratios of Bacteroides sp., Bifidobacterium sp., Clostridium leptum, Clostridium coccoides, Streptococcus sp., Lactobacillus sp. and Enterobacteriacaea can be investigated in autopsy rectum samples up to 5 days after death. Keywords: Forensic science, Post mortem microbiology, Fecal sample, Real-time quantitative polymerase chain reaction, Bacterial relative amount, Time-dependent changes, Author(s): Sari Tuomisto[sup.1] , Pekka J Karhunen[sup.1,2] and Tanja Pessi[sup.1,2] Background Basic knowledge on the composition of intestinal bacterial populations and changes occurring after death is lacking. Even the normal [...]
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- 2013
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11. Signs of Periodontal Pathogens in Acute Arterial Lower Limb Thrombotic Events
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Vakhitov, Damir, Tuomisto, Sari, Korhonen, Janne, Salenius, Juha-Pekka, Suominen, Velipekka, Pekka, Karhunen, and Oksala, Niku
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- 2019
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12. Oral Bacterial Signatures in Cerebral Thrombi of Patients With Acute Ischemic Stroke Treated With Thrombectomy.
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Patrakka, Olli, Pienimäaki, Juha-Pekka, Tuomisto, Sari, Ollikainen, Jyrki, Lehtimäki, Terho, Karhunen, Pekka J., Martiskainen, Mika, and Pienimäki, Juha-Pekka
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- 2019
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13. Suoliston bakteerit: kuoleman jälkeiset muutokset, kulkeutuminen ja yhteys alkoholimaksakirroosiin
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Tuomisto, Sari, Lääketieteen yksikkö - School of Medicine, and University of Tampere
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Suolistobakteeri ,alkoholimaksakirroosi ,post-mortem bacterology ,alcoholic liver cirrhosis ,Intestinal bacteria ,Oikeuslääketiede/molekyylibiologia - Forensic Medicine/Molecular Biology ,post-mortem bacteriologia - Abstract
Suoliston bakteerit: kuoleman jälkeiset muutokset, kulkeutuminen ja yhteys alkoholimaksakirroosiin Suoliston mikrobioston, jonka on arvioitu koostuvan noin 10 14 bakteerista, tiedetään vaikuttavan isäntänsä hyvinvointiin ja se on myös liitetty erilaisiin sairauksiin kuten alkoholimaksakirroosiin. On oletettu, että alkoholi muuttaa suoliston bakteeriston koostumusta ja lisää bakteerien kulkeutumista suolistosta maksaan. Nämä bakteerit ja niiden jäännökset voisivat sitten aiheuttaa maksassa kroonisen tulehdustilan, joka voi lopulta johtaa kirroosiin. Kuoleman jälkeen bakteerit alkavat siirtyä elimiin osana luonnollista hajoamisprosessia. Bakteerien siirtymistä suolen ontelosta vereen ja sisäelimiin tapahtuu normaalisti myös elämän aikana, joskin immuunijärjestelmä hankkiutuu näistä bakteereista eroon. Kuoleman jälkeisiä suoliston bakteeriryhmien muutoksia ja näiden bakteerien kulkeutumista elimiin on tutkittu vain vähän. Tämän vuoksi ei tiedetä, voidaanko kuoleman jälkeen otetuilla näytteillä tutkia elämän aikaisia tapahtumia. Tämän väitöskirjatyön tarkoituksena on selvittää miten suoliston bakteeristo kuoleman jälkeen muuttuu ajan mukaan sekä miten nämä bakteerit kulkeutuvat kudoksiin. Sovelsimme näitä saatuja tuloksia suolistobakteerien roolin määrittämisessä alkoholimaksakirroosissa. Tuloksemme osoittavat ruumiinavausten peräsuolinäytteiden verrattavuuden elävien ihmisten ulostenäytteisiin. Tiettyjen bakteeriryhmien määrät eivät muuttuneet, joten kuoleman jälkeen otettuja ulostenäytteitä voidaan käyttää näiden bakteeriryhmien tutkimuksessa. Parhaat ja luotettavimmat kudosten näytteenottopaikat mikrobiologisiin tarkoituksiin olivat sydänpussineste ja maksa aina 5 päivään saakka kuoleman jälkeen. Maksaa ja sydänpussinestettä voidaan käyttää erilaisten elämänaikaisten tautimekanismien tutkimuksessa ja sydänpussinestettä jopa mikrobiologisena kontrollina kuoleman jälkeiselle kontaminaatiolle ja bakteerien kulkeutumiselle. Jos näistä näytteistä löytyy yksittäisiä bakteereita, niiden voidaan ajatella edustavan oikeaa elämänaikaista infektiota. Tutkimustuloksemme ehdottavat myös suoliston bakteereilla olevan rooli alkoholimaksakirroosin synnyssä. Löysimme lisääntyneen määrän Enterobactericeae-heimon bakteereja kirrootikkojen ulosteista, maksoista ja askitesnestenäytteistä. Kirrootikoilla oli myös lisääntynyt ilmentymisprosentti CD14 bakteerien tunnistus reseptorille; yhteys CD14 ja kokonaisbakteerimäärän välillä nähtiin kirrootikkojen maksanäytteissä. Tuloksemme viittaavatkin, että suoliston haitallisten Enterobactericeae-heimon bakteerien määrän kasvu voi johtaa niiden kulkeutumiseen maksaan ja lisääntyneeseen tunnistamiseen siellä Kupfferin solujen toimesta CD14 reseptorien kautta. Kupfferin solujen sytokiinien liikatuotanto voi johtaa maksan sidekudoistumiseen ja mahdollisesti jopa kirroosiin. The intestinal microbiota, that has been estimated to consist of around 10 14 bacteria, is known to affect the well-being of the host, and has been linked to various diseases such as alcoholic liver cirrhosis. Alcohol has been hypothesised to alter intestinal bacterial populations and enhance bacterial migration from the intestine into the liver. These bacteria and their residuals could then promote chronic inflammation that could eventually lead to fibrosis and cirrhosis. After death, bacteria start to migrate into the organs as a part of normal putrefaction. Bacterial migration, i.e., translocation from the intestinal lumen into the blood and visceral organs, occurs also normally during life. The time sequence of post-mortem changes in intestinal bacterial populations and their migration into organs has not been extensively studied. Therefore, it is not known whether results obtained from post-mortem samples reflect conditions during life. The aim of this doctoral thesis was to examine time-dependent changes in the intestinal microbiota after death and the translocation of these bacteria into tissues. Finally, we used these results to evaluate the role of intestinal bacteria in alcoholic liver cirrhosis. Post-mortem changes in major intestinal bacterial groups (Bacteroides spp., Bifidobacterium spp., Clostridium coccoides, Clostridium leptum group, Enterobactericeae, Lactobacillus spp.) and Streptococcus spp. were evaluated in 61 rectal and ceacal samples obtained at autopsy and in 7 healthy volunteers using quantitative real-time PCR (RT-qPCR, Study I). Bacterial migration after death into the liver, mesenteric lymph nodes, pericardium, portal vein and peripheral blood was examined in a time-dependent manner with RT-qPCR and conventional bacterial culturing in 33 post-mortem samples. RT-qPCR was used to study the following bacterial groups: Bacteroides spp., Bifidobacterium spp., Clostridium leptum group, Clostridium coccoides group, Enterobacter spp., Staphylococcus spp. and Streptococcus spp. (Study II). The composition of selected intestinal bacteria (Bacteroides spp., Bifidobacterium spp., Clostridium leptum group, Enterobactericeae and Lactobacillus spp.) was investigated in 42 rectal samples obtained at autopsy (13 cirrhotics, 15 alcoholics, and 14 controls) and in faecal samples from 7 healthy volunteers (Study III). Intestinal bacterial translocation into the liver (n=42) and its association with cirrhosis and ascites (n=12) was also studied with RT-qPCR and CD14 immunohistochemistry. Our results show that the relative amounts of Bacteroides spp., Bifidobacterium spp., Clostridium spp., Streptococcus spp., Enterobactericeae and Lactobacillus spp. remained stable in the rectum for up to 5 days after death (Study I). The proportions of the major intestinal bacteria populations in rectal autopsy samples were comparable to faecal samples from living persons. In contrast, post-mortem caecal samples proved to be microbiologically unreliable, as significant time-dependent post-mortem differences were observed in bacterial populations of Bacteroides spp. (p=0.014) and Lactobacillus spp. (p=0.024, Study I). Bacterial culturing and RT-qPCR analyses revealed the presence of bacteria in peripheral blood soon after death as a result of post-mortem bacterial migration. Outside the intestinal tract, the most reliable post-mortem microbiological sampling sites at up to 5 days after death were the pericardial fluid and the liver (Study II). Based on the results of Studies I and II, cases over 5 days post-mortem were excluded from Study III, and RT-qPCR measurements of Bacteroides spp., Bifidobacterium spp., Clostridium leptum group, Enterobactericeae and Lactobacillus spp. were selected for the samples. Alcoholic liver cirrhotics harboured 27 times more DNA of Enterobactericeae in their faeces than living controls (p=0.011). Genomes of this bacterial species were also found in the liver and ascites samples from cirrhotics (Study III). The total amount of bacterial DNA was associated with the level of CD14 expression (p=0.045). Furthermore, the CD14 expression percentage was statistically significantly higher in cirrhotic livers than in autopsy controls (p=0.004, Study III). In conclusion, post-mortem samples from the rectum and liver can be used in basic research if taken within 5 days after death. Alcoholic cirrhotics harboured more gram-negative Enterobactericeae in their faeces and had higher amounts of Enterobactericeae in the liver. This suggests the possible involvement of intestinal bacteria in the development of alcoholic liver cirrhosis.
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- 2014
14. Bacterial signatures in thrombus aspirates of patients with lower limb arterial and venous thrombosis.
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Vakhitov, Damir, Tuomisto, Sari, Martiskainen, Mika, Korhonen, Janne, Pessi, Tanja, Salenius, Juha-Pekka, Suominen, Velipekka, Lehtimäki, Terho, Karhunen, Pekka J., and Oksala, Niku
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Objective Increasing data supports the role of bacterial inflammation in adverse events of cardiovascular and cerebrovascular diseases. In our previous research, DNA of bacterial species found in coronary artery thrombus aspirates and ruptured cerebral aneurysms were mostly of endodontic and periodontal origin, where Streptococcus mitis group DNA was the most common. We hypothesized that the genomes of S mitis group could be identified in thrombus aspirates of patients with lower limb arterial and deep venous thrombosis. Methods Thrombus aspirates and control blood samples taken from 42 patients with acute or acute-on-chronic lower limb ischemia (Rutherford I-IIb) owing to arterial or graft thrombosis (n = 31) or lower limb deep venous thrombosis (n = 11) were examined using a quantitative real-time polymerase chain reaction to detect all possible bacterial DNA and DNA of S mitis group in particular. The samples were considered positive, if the amount of bacterial DNA in the thrombus aspirates was 2-fold or greater in comparison with control blood samples. Results In the positive samples the mean difference for the total bacterial DNA was 12.1-fold (median, 7.1), whereas the differences for S mitis group DNA were a mean of 29.1 and a median of 5.2-fold. Of the arterial thrombus aspirates, 57.9% were positive for bacterial DNA, whereas bacterial genomes were found in 75% of bypass graft thrombosis with 77.8% of the prosthetic grafts being positive. Of the deep vein thrombus aspirates, 45.5% contained bacterial genomes. Most (80%) of bacterial DNA-positive cases contained DNA from the S mitis group. Previous arterial interventions were significantly associated with the occurrence of S mitis group DNA ( P = .049, Fisher's exact test). Conclusions This is the first study to report the presence of bacterial DNA, predominantly of S mitis group origin, in the thrombus aspirates of surgical patients with lower limb arterial and deep venous thrombosis, suggesting their possible role in the pathogenesis of thrombotic events. Additional studies will, however, be needed to reach a final conclusion. [ABSTRACT FROM AUTHOR]
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- 2018
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15. Component screening for novel xeno-free medium for human embryonic stem cells
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Tuomisto, Sari
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hESC ,culture of hESC ,ihminen ,alkio ,xeno-free ,kantasolut - Published
- 2007
16. Endothelial Nitric Oxide Synthase G894T Polymorphism Associates with Disease Severity in Puumala Hantavirus Infection.
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Koskela, Sirpa, Laine, Outi, Mäkelä, Satu, Pessi, Tanja, Tuomisto, Sari, Huhtala, Heini, Karhunen, Pekka J., Pörsti, Ilkka, and Mustonen, Jukka
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NITRIC-oxide synthases ,ENDOTHELIAL cells ,HANTAVIRUS diseases ,ENDOTHELIUM diseases ,CREATININE ,BLOOD pressure ,GENETIC polymorphisms - Abstract
Introduction: Hantavirus infections are characterized by both activation and dysfunction of the endothelial cells. The underlying mechanisms of the disease pathogenesis are not fully understood. Here we tested the hypothesis whether the polymorphisms of endothelial nitric oxide synthase, eNOS G894T, and inducible nitric oxide synthase, iNOS G2087A, are associated with the severity of acute Puumala hantavirus (PUUV) infection. Patients and Methods: Hospitalized patients (n = 172) with serologically verified PUUV infection were examined. Clinical and laboratory variables reflecting disease severity were determined. The polymorphisms of eNOS G894T (Glu298Asp, rs1799983) and iNOS G2087A (Ser608Leu, rs2297518) were genotyped. Results: The rare eNOS G894T genotype was associated with the severity of acute kidney injury (AKI). The non-carriers of G-allele (TT-homozygotes) had higher maximum level of serum creatinine than the carriers of G-allele (GT-heterozygotes and GG-homozygotes; median 326, range 102–1041 vs. median 175, range 51–1499 μmol/l; p = 0.018, respectively). The length of hospital stay was longer in the non-carriers of G-allele than in G-allele carriers (median 8, range 3–14 vs. median 6, range 2–15 days; p = 0.032). The rare A-allele carriers (i.e. AA-homozygotes and GA-heterozygotes) of iNOS G2087A had lower minimum systolic and diastolic blood pressure than the non-carriers of A-allele (median 110, range 74–170 vs.116, range 86–162 mmHg, p = 0.019, and median 68, range 40–90 vs. 72, range 48–100 mmHg; p = 0.003, respectively). Conclusions: Patients with the TT-homozygous genotype of eNOS G894T had more severe PUUV-induced AKI than the other genotypes. The eNOS G894T polymorphism may play role in the endothelial dysfunction observed during acute PUUV infection. [ABSTRACT FROM AUTHOR]
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- 2015
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17. Changes in gut bacterial populations and their translocation into liver and ascites in alcoholic liver cirrhotics.
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Tuomisto, Sari, Pessi, Tanja, Collin, Pekka, Vuento, Risto, Aittoniemi, Janne, and Karhunen, Pekka J.
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BIOLOGICAL transport , *CIRRHOSIS of the liver , *ALCOHOLIC liver diseases , *STAINS & staining (Microscopy) , *ENTEROBACTERIACEAE , *CLOSTRIDIUM , *LACTOBACILLUS , *BACTERIA - Abstract
Background The liver is the first line of defence against continuously occurring influx of microbialderived products and bacteria from the gut. Intestinal bacteria have been implicated in the pathogenesis of alcoholic liver cirrhosis. Escape of intestinal bacteria into the ascites is involved in the pathogenesis of spontaneous bacterial peritonitis, which is a common complication of liver cirrhosis. The association between faecal bacterial populations and alcoholic liver cirrhosis has not been resolved Methods Relative ratios of major commensal bacterial communities (Bacteroides spp., Bifidobacterium spp., Clostridium leptum group, Enterobactericaea and Lactobacillus spp.) were determined in faecal samples from post mortem examinations performed on 42 males, including cirrhotic alcoholics (n = 13), non-cirrhotic alcoholics (n = 15), non-alcoholic controls (n = 14) and in 7 healthy male volunteers using real-time quantitative PCR (RTqPCR). Translocation of bacteria into liver in the autopsy cases and into the ascites of 12 volunteers with liver cirrhosis was also studied with RT-qPCR. CD14 immunostaining was performed for the autopsy liver samples. Results Relative ratios of faecal bacteria in autopsy controls were comparable to those of healthy volunteers. Cirrhotics had in median 27 times more bacterial DNA of Enterobactericaea in faeces compared to the healthy volunteers (p = 0.011). Enterobactericaea were also the most common bacteria translocated into cirrhotic liver, although there were no statistically significant differences between the study groups. Of the ascites samples from the volunteers with liver cirrhosis, 50% contained bacterial DNA from Enterobactericaea, Clostridium leptum group or Lactobacillus spp.. The total bacterial DNA in autopsy liver was associated with the percentage of CD14 expression (p = 0.045). CD14 expression percentage in cirrhotics was significantly higher than in the autopsy controls (p = 0.004). Conclusions Our results suggest that translocation of intestinal bacteria into liver may be involved as a one factor in the pathogenesis of alcoholic liver cirrhosis. [ABSTRACT FROM AUTHOR]
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- 2014
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18. High prevalence of lactase non-persistence among indigenous nomadic Nenets, north-west Russia.
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Khabarova, Yulia, Grigoryeva, Valentina, Tuomisto, Sari, Karhunen, PekkaJ, Mattila, Kari, and Isokoski, Mauri
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LACTASE persistence ,LACTOSE intolerance ,INDIGENOUS peoples ,NENTSY - Abstract
Objectives. The frequency of adult-type hypolactasia (lactase non-persistence) varies widely among different ethnic groups. The cultural historical hypothesis assumes a link between the occurrence of hypolactasia and the distribution of dairy farming. The nomadic Nenets have been reindeer herders for generations and have therefore not consumed any dairy products. The hypotheses here was that the prevalence of lactase nonpersistence (-13910 C/C genotype) among Nenets people having four Nenets grandparents is high, while the prevalence among Nenets originating from ethnically mixed families is lower. Study design. The material was collected in four typical Nenets settlements in the Nenets Autonomous Okrug in Russia. One-third of the adult Nenets population were invited to answer a questionnaire and to donate buccal samples for genotyping by a doctor from the team of medical professionals who make rounds in this area. The total number of available participants was 177. Methods. Genotyping was performed with the AbiPrism system. We used the method of concordance of grandparents' national origin to ascribe ethnicity. Results. The prevalence of adult-type hypolactasia (-13910 C/C) among Nenets who had four Nenets grandparents was found to be 90%. The figures among others reporting three, two and one grandparent of Nenets origin were 72, 60 and 28%, respectively. Conclusion. The findings are in accord with the cultural historical hypothesis. [ABSTRACT FROM AUTHOR]
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- 2012
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19. Oral bacterial DNA findings in pericardial fluid.
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Louhelainen AM, Aho J, Tuomisto S, Aittoniemi J, Vuento R, Karhunen PJ, and Pessi T
- Abstract
Background: We recently reported that large amounts of oral bacterial DNA can be found in thrombus aspirates of myocardial infarction patients. Some case reports describe bacterial findings in pericardial fluid, mostly done with conventional culturing and a few with PCR; in purulent pericarditis, nevertheless, bacterial PCR has not been used as a diagnostic method before., Objective: To find out whether bacterial DNA can be measured in the pericardial fluid and if it correlates with pathologic-anatomic findings linked to cardiovascular diseases., Methods: Twenty-two pericardial aspirates were collected aseptically prior to forensic autopsy at Tampere University Hospital during 2009-2010. Of the autopsies, 10 (45.5%) were free of coronary artery disease (CAD), 7 (31.8%) had mild and 5 (22.7%) had severe CAD. Bacterial DNA amounts were determined using real-time quantitative PCR with specific primers and probes for all bacterial strains associated with endodontic disease (Streptococcus mitis group, Streptococcus anginosus group, Staphylococcus aureus/Staphylococcus epidermidis, Prevotella intermedia, Parvimonas micra) and periodontal disease (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatus, and Dialister pneumosintes)., Results: Of 22 cases, 14 (63.6%) were positive for endodontic and 8 (36.4%) for periodontal-disease-associated bacteria. Only one case was positive for bacterial culturing. There was a statistically significant association between the relative amount of bacterial DNA in the pericardial fluid and the severity of CAD (p=0.035)., Conclusions: Oral bacterial DNA was detectable in pericardial fluid and an association between the severity of CAD and the total amount of bacterial DNA in pericardial fluid was found, suggesting that this kind of measurement might be useful for clinical purposes.
- Published
- 2014
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