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Cystic-like structures similar to xenomas containing ovoid or piriform organisms were observed in brain and kidney of a one-month-old rabbit. Different dyes such as H&E, PAS, toluidine blue and trichrome blue were tested. Trichrome blue yielded the best results in terms of specificity, resolution and confirmation of microsporidia spores. This dye is easily adaptable to paraffin-embedded samples by not requiring additional equipment; it is rapid, cheap and could be used as a confirmatory technique for other microsporidiosis. [ABSTRACT FROM AUTHOR]

The multilobular tumor of bone (MTB) is considered as a slow-growth malignant tumor, which generally occurs on the cranium and facial bones of dogs, although it has been reported in other domestic animals. This study presents two cases of MTB in dogs. The first case was manifested in an 8-year-old male Bull Terrier, while the second one affected a 5.5-year-old female German Shepherd. Both animals were submitted for post-mortem examination. MTB diagnosis was confirmed by histopathological examination. The aim of this report was to describe the main clinical-pathologic features of MTB, such as its slow, diffuse and deforming growth pattern, as well as the histomorphological image peculiarities that are unmistakable from other tumors such as chondrosarcoma and osteosarcoma, that should be considered as differential diagnoses. Up to date, this is the first report of canine MTB in Mexico. [ABSTRACT FROM AUTHOR]

A case of myositis ossificans circumscripta in an eight-year-old, male, Weimaraner dog, is described. The animal was originally submitted with presumptive diagnosis of hernia. The lesion was a firm, subcutaneous nodule, 5 cm long by 3 cm width, localized in the right perineum muscles. After the radiologic study the lesion was interpreted as a neoplasm from coccygeal vertebrae. The surgical procedure to eliminate the neoplastic tissue confirmed its hardness. Excision was not complete and the sample was submitted to pathology. The mass was hard, well circumscribed, vascularized and surrounded by muscle tissue. The histopathological study revealed a heterotopic ossification with zones of atrophy, degeneration and muscle necrosis, alternating with zones of trabecular ossification toward the periphery, limited by abundant fibrous connective tissue. These findings are characteristic of myositis ossificans circumscripta. This case represents the first report in a dog in Mexico, of a condition resembling the myositis ossificans circumscripta in humans. [ABSTRACT FROM AUTHOR]