1. Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge
- Author
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Tomoaki Takamoto, Toshio Kawase, Hatsuhiko Maeda, Yosuke Hiraoka, Hideyuki Negishi, Emiko Sato, Yasuhiko Tabata, Yoichiro Kameyama, Yukihiko Kinoshita, Motoki Fujita, and Satoshi Ozono
- Subjects
Male ,Stromal cell ,Bone Regeneration ,Swine ,Ultraviolet Rays ,Basic fibroblast growth factor ,Anti-Inflammatory Agents ,Cell Culture Techniques ,Bone Marrow Cells ,Cell Count ,Rat Bone Marrow ,Collagen Type I ,Dexamethasone ,Tendons ,chemistry.chemical_compound ,Coated Materials, Biocompatible ,medicine ,Cell Adhesion ,Animals ,Glycolic acid ,Cells, Cultured ,Cell Proliferation ,Tissue Engineering ,General Engineering ,Cell Differentiation ,Alkaline Phosphatase ,Molecular biology ,Rats, Inbred F344 ,Rats ,Drug Combinations ,chemistry ,Biochemistry ,Collagen sponge ,Microscopy, Electron, Scanning ,Fibroblast Growth Factor 2 ,Stromal Cells ,Polyglycolic Acid ,medicine.drug - Abstract
We studied the effects of dexamethasone (Dex) and basic fibroblast growth factor (bFGF) on proliferation and differentiation of rat bone marrow stromal cells (RBMSCs), using three scaffolds: collagen sponge, poly(glycolic acid) (PGA)-collagen sponge, and PGA-collagen (UV) sponge. RBMSCs were seeded into the sponges, and cultured in primary medium, primary medium with Dex, and primary medium with bFGF and Dex. Three weeks after cultivation, we examined alkaline phosphatase (ALP) activity and cell number in the sponges, and also performed macroscopic, light microscopic, and scanning electron microscopic (SEM) observations. Collagen sponge shrank considerably, but PGA-collagen and PGA-collagen (UV) sponges maintained most of their original shape. PGA-collagen (UV) sponge supplemented with bFGF and Dex together had the highest ALP activity and cell number, followed by PGA-collagen sponge. Although collagen sponge showed cell proliferation only on the surface, the other two sponges showed cell proliferation in the interior. SEM showed the best cell attachment to PGA-collagen (UV) sponge in the presence of bFGF and Dex, followed by PGA-collagen sponge. In conclusion, PGA-collagen (UV) and PGA-collagen sponges proved to be much more useful as scaffolding for bone regeneration when combined with bFGF and Dex.
- Published
- 2005