Search

Anaplasma marginale and piroplasm species are widespread among Brazilian cattle herds. Both of these tick-borne pathogens hamper livestock production and cause a significant economic impact. Although buffaloes have demonstrated a high level of adaptability, data on tick-borne pathogens are scarcely reported in Brazil. Thus, the aim of this study was to screen water buffaloes from the state of Maranhão for piroplasm and A. marginale occurrence using PCR assays. All samples were negative for A. marginale. One of the 287 (0.35%) water buffaloes tested was positive for Theileria sp. Sequencing of the 18S rDNA fragment (356 bp) showed that the Theileria sp. identified was closely related to the T. buffeli /orientalis group. Future studies on the clinical signs of infection and the main vector in this country are needed.

The aim of this research was to identify and to find out the prevalence of blood parasites in cattle in Kutalimbaru Subdistrict, Deli Serdang Regency. This research was conducted in Kutalimbaru Subdistrict, Deli Serdang Regency from December 2018 to February 2019. Normal/healthy cattle of different breeds were randomly selected from five locations, namely Sei Mencirim village, Sawit Rejo village, Silebo-lebo village, Sampe Cita village and Pasar X village. A total of 150 blood samples were collected for blood smears. Diagnostic techniques were tested using Giemsa's staining technique. The blood parasites were then identified and their prevalence was determined. The results showed that one species of blood parasite was successfully identified, all belonging to the blood parasite, namely, Theileria sp. with a prevalence of 33%. In addition, there were no species of blood parasite from the Anaplasma, Trypanosoma, and Babesia. This study suggests that theileriosis is spreading to even the other region in North Sumatera. To reduce the infection, follow up surveys of the blood parasites of the livestock, assess their distribution and infection rates of possible vectors are recommended and plans control measures against should be focused on reducing transmission to highly susceptible animal species.

The aim of this research was to determine the type and prevalence of blood protozoa disease on cattle and buffalo in Moyo Hilir sub‐district, Sumbawa District, Wes Nusa Tenggara. This research was taken on july until December 2018. This research used a nonexperimental method through an observation study. The samples of this research were made in thin blood smear using 20% Giemsa’s stain and all samples were from 200 blood of cattle and buffalo in Moyo Hilir sub‐district, Sumbawa District, West Nusa Tenggara. The results showed that the prevalence of blood protozoa disease was 11.5 Based on the type of blood protozoa, the highest prevalence was Anaplasma sp. (11%), followed by Theileria sp. (0.5%), while Babesia sp. And Trypanosoma sp. Were not found. Based on the livestock types, the prevalence in buffalo was higher (17%)than cttle (6%). Based on sex the prevalence in female was higher (12.6%) than male (10.11%). Based on age of livestock, the prevalance of livestock with age > 2 years old was higher (13.33%) than livestock with age ≤ 2 years old (10.4%) based on the location of the village, blood protozoa disease were only found in Olatrawa village (17.85%) and Serading village (17.82%), while in Kakiang and Moyo village were not found. The conclusion of this research indicate that prevalence of blood protozoa disease was 11.5% and only Anaplasma sp. And Theileria sp. Were found. Key words : anaplasmosis, babesiosis, theileriosis, trypanosomiasis, Sumbawa District.

application/pdf, 全国8地域のニホンジカに寄生するタイレリアの18S rRNAおよびp33/34遺伝子を解析した. 18S rRNA遺伝子の相同性は99.7-100%であったが,山口県産ニホンジカのタイレリアp33/34遺伝子の相同性は,他地域のものに比べて塩基配列で68.7〜70.1%,アミノ酸配列で64,1〜70.0%と低く,ニホンジカのタイレリアには遺伝的に異なる株が存在することが示唆された., The 18S rRNA gene and the piroplasm major immunodominant protein gene (p33/34) of Theileria from various subspecies of sika deer in 8 different locations of Japan were analyzed. The similarity between 633 bp partial sequences of the 18S rRNA gene among various subspecies of sika deer was found to be between 99.7% and 100%. While the percent identities of the 412 bp partial p33/34 gene sequence and deduced amino acid sequences between Theileria of sika deer from Yamaguchi Prefecture and those found in deer from other Prefectures, were comparatively low, 68.7% to 70.1% and 64.1% to 70.0% respectively. These findings suggest that there are at least two genetically distinct strains of Theileria of sika deer in Japan.

Theileriaspp. are tick-transmitted, intracellular apicomplexan protozoan parasites infecting a wide range of animals. As there is very limited information on the prevalence ofTheileriaspp. in the Caribbean we used the recently described genus-specific pan-TheileriaFRET-qPCR to identify infected animals in the region and a standard 18S rRNA gene PCR and sequencing to determine the species involved. We foundTheileriaspp. in 9% of the convenience samples of animals (n=752) studied from five Caribbean islands. Donkeys (20.0%: 5/25) were most commonly infected, followed by sheep (17.4%, 25/144), cattle (6.8%; 22/325), goats (5.0%; 12/238), and horses (5.0%; 1/20). Six species ofTheileriawere identified:T.equi(donkeys, cattle, goats, and sheep),Theileriasp. OT3 (sheep and goats),Theileriasp. NG-2013a (cattle),Theileriasp. YW-2014 (donkeys),Theileriasp. B15a (goats), andBabesia vulpesor a closely related organism (sheep and goats). OnlyT. equihas been previously reported in the Caribbean. Our findings expand the known host ranges ofTheileriaspp. and the known distribution of the organisms around the world.

Theileriosis is a tick-borne disease caused by a piroplasma of the genus Theileria that can causeanaemia and thrombocytopenia. Its clinical importance for dogs’ remains poorly understood,as only some develop clinical signs. In this study, physical and laboratory findings, treatment and outcomes of six client-owned diseased dogs presented at the Onderstepoort Veterinary Academic Hospital are described retrospectively. In the dogs, Theileria species (n = 4)and Theileria equi (n = 2) were detected by a polymerase chain reaction (PCR)-reverse blothybridisation assay in blood samples, whilst PCR for Babesia, Anaplasma and Ehrlichia were negative. The most common physical findings were pale mucous membranes (five out of six dogs), bleeding tendencies (five out of six dogs) and lethargy (three out of six dogs). All dogs were thrombocytopenic [median 59.5 x 109/L (range 13–199)] and five out of six dogs were anaemic [median haematocrit 18% (range 5–32)]. Bone marrow core biopsies performed in two dogs showed myelofibrosis. Theileriosis was treated with imidocarb dipropionate and the suspected secondary immune-mediated haematological disorders with prednisolone and azathioprine. Five dogs achieved clinical cure and post-treatment PCR performed in three out of five dogs confirmed absence of circulating parasitaemia. An immune-mediated response to Theileria species is thought to result in anaemia and/or thrombocytopenia in diseased dogs with theileriosis. A bleeding tendency, most likely secondary to thrombocytopenia and/or thrombocytopathy, was the most significant clinical finding in these cases. The link between thrombocytopenia, anaemia and myelofibrosis in theileriosis requires further investigation and theileriosis should be considered a differential diagnosis for dogs presenting with anaemia and/or thrombocytopenia in endemic tick-borne disease areas.

An epizootiological field study on tick-borne protozoan infections in foxes (Vulpes vulpes) was carried out in different parts of Croatia. Spleen samples of 191 carcasses of red foxes killed in sanitary hunting, were examined for the presence of hematozoa by polymerase chain reaction (PCR) and subsequent sequencing. The investigation revealed four species of hematozoa in 57 foxes (30%), namely Theileria annae, Theileria sp. 3182/05 and Hepatozoon canis. Theileria annae was found in 10 foxes (5%), Theileria sp. 3182/05 in a single animal (1%), H. canis in 44 (23%) and Hepatozoon sp. was detected in two foxes (1%). T. annae and H. canis were distributed through all the studied regions, while Theileria sp. 3182/05 and Hepatozoon sp. were restricted to the Zagreb and Zagorje, and Istria regions, respectively. Detection of T. annae in all regions of Croatia indicates the presence of the natural cycle of the parasite and raises the possibility of other vectors other than the proposed Ixodes hexagonus.

application/pdf