10 results on '"Tavaddod, Sharareh"'
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2. Morphometry and Modeling of Label-Free Human Melanocytes and Melanoma Cells
- Author
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Tavaddod, Sharareh, Shojaedin-Givi, Behnaz, Mahmoudi-Rad, Mahnaz, and Naderi-Manesh, Hossein
- Published
- 2021
- Full Text
- View/download PDF
3. Synergistic effect of co-immobilized FGF-2 and vitronectin-derived peptide on feeder-free expansion of induced pluripotent stem cells
- Author
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Sohi, Alireza Naderi, Naderi-Manesh, Hossein, Soleimani, Masoud, Yasaghi, Elaheh Roshani, Manjili, Hamidreza Kheiri, Tavaddod, Sharareh, and Nojehdehi, Shahrzad
- Published
- 2018
- Full Text
- View/download PDF
4. In Vivo study of naturally deformed Escherichia coli bacteria
- Author
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Tavaddod, Sharareh and Naderi-Manesh, Hossein
- Published
- 2016
- Full Text
- View/download PDF
5. Stability of β-lactam antibiotics in bacterial growth media.
- Author
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Brouwers, Rebecca, Vass, Hugh, Dawson, Angela, Squires, Tracey, Tavaddod, Sharareh, and Allen, Rosalind J.
- Subjects
BACTERIAL growth ,AZTREONAM ,BETA lactam antibiotics ,ANTIBIOTICS ,CEFOTAXIME ,ANTIBIOTICS assay - Abstract
Laboratory assays such as MIC tests assume that antibiotic molecules are stable in the chosen growth medium—but rapid degradation has been observed for antibiotics including β-lactams under some conditions in aqueous solution. Degradation rates in bacterial growth medium are less well known. Here, we develop a 'delay time bioassay' that provides a simple way to estimate antibiotic stability in bacterial growth media, using only a plate reader and without the need to measure the antibiotic concentration directly. We use the bioassay to measure degradation half-lives of the β-lactam antibiotics mecillinam, aztreonam and cefotaxime in widely-used bacterial growth media based on MOPS and Luria-Bertani (LB) broth. We find that mecillinam degradation can occur rapidly, with a half-life as short as 2 hours in MOPS medium at 37°C and pH 7.4, and 4-5 hours in LB, but that adjusting the pH and temperature can increase its stability to a half-life around 6 hours without excessively perturbing growth. Aztreonam and cefotaxime were found to have half-lives longer than 6 hours in MOPS medium at 37°C and pH 7.4, but still shorter than the timescale of a typical minimum inhibitory concentration (MIC) assay. Taken together, our results suggest that care is needed in interpreting MIC tests and other laboratory growth assays for β-lactam antibiotics, since there may be significant degradation of the antibiotic during the assay. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
6. D, L-Sulforaphane Loaded Fe3O4@ Gold Core Shell Nanoparticles: A Potential Sulforaphane Delivery System.
- Author
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Kheiri Manjili, Hamidreza, Ma’mani, Leila, Tavaddod, Sharareh, Mashhadikhan, Maedeh, Shafiee, Abbas, and Naderi-Manesh, Hossein
- Subjects
DRUG delivery systems ,SULFORAPHANE ,IRON oxide nanoparticles ,GOLD coatings ,FABRICATION (Manufacturing) ,ANTINEOPLASTIC agents ,DRUG stability - Abstract
A novel design of gold-coated iron oxide nanoparticles was fabricated as a potential delivery system to improve the efficiency and stability of d, l-sulforaphane as an anticancer drug. To this purpose, the surface of gold-coated iron oxide nanoparticles was modified for sulforaphane delivery via furnishing its surface with thiolated polyethylene glycol-folic acid and thiolated polyethylene glycol-FITC. The synthesized nanoparticles were characterized by different techniques such as FTIR, energy dispersive X-ray spectroscopy, UV-visible spectroscopy, scanning and transmission electron microscopy. The average diameters of the synthesized nanoparticles before and after sulforaphane loading were obtained ∼ 33 nm and ∼ 38 nm, respectively, when ∼ 2.8 mmol/g of sulforaphane was loaded. The result of cell viability assay which was confirmed by apoptosis assay on the human breast cancer cells (MCF-7 line) as a model of in vitro-cancerous cells, proved that the bare nanoparticles showed little inherent cytotoxicity, whereas the sulforaphane-loaded nanoparticles were cytotoxic. The expression rate of the anti-apoptotic genes (bcl-2 and bcl-x
L ), and the pro-apoptotic genes (bax and bak) were quantified, and it was found that the expression rate of bcl-2 and bcl-xL genes significantly were decreased when MCF-7 cells were incubated by sulforaphane-loaded nanoparticles. The sulforaphane-loaded into the designed gold-coated iron oxide nanoparticles, acceptably induced apoptosis in MCF-7 cells. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
7. A MATLAB program to calculate translational and rotational diffusion coefficients of a single particle
- Author
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Charsooghi, Mohammad A., Akhlaghi, Ehsan A., Tavaddod, Sharareh, and Khalesifard, H.R.
- Subjects
- *
GRAPHICAL user interfaces , *DIFFUSION , *PARTICLES , *COMPUTER software , *DIMENSIONAL analysis , *FLUID dynamics , *AUTOCORRELATION (Statistics) - Abstract
Abstract: We developed a graphical user interface, MATLAB based program to calculate the translational diffusion coefficients in three dimensions for a single diffusing particle, suspended inside a fluid. When the particles are not spherical, in addition to their translational motion also a rotational freedom is considered for them and in addition to the previous translational diffusion coefficients a planar rotational diffusion coefficient can be calculated in this program. Time averaging and ensemble averaging over the particle displacements are taken to calculate the mean square displacement variations in time and so the diffusion coefficients. To monitor the random motion of non-spherical particles a reference frame is used that the particle just have translational motion in it. We call it the body frame that is just like the particle rotates about the z-axis of the lab frame. Some statistical analysis, such as velocity autocorrelation function and histogram of displacements for the particle either in the lab or body frames, are available in the program. Program also calculates theoretical values of the diffusion coefficients for particles of some basic geometrical shapes; sphere, spheroid and cylinder, when other diffusion parameters like temperature and fluid viscosity coefficient can be adjusted. Program summary: Program title: KOJA Catalogue identifier: AEHK_v1_0 Program summary URL: http://cpc.cs.qub.ac.uk/summaries/AEHK_v1_0.html Program obtainable from: CPC Program Library, Queen''s University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 48 021 No. of bytes in distributed program, including test data, etc.: 1 310 320 Distribution format: tar.gz Programming language: MatLab (MathWorks Inc.) version 7.6 or higher. Statistics Toolbox and Curve Fitting Toolbox required. Computer: Tested on windows and linux, but generally it would work on any computer running MatLab (MathWorks Inc.). There is a bug in windows 7, if the user is not the administrator sometimes the program was not able to overwrite some internal files. Operating system: Any supporting MatLab (MathWorks Inc.) v7.6 or higher. RAM: About eight times that of loaded data Classification: 12 Nature of problem: In many areas of physics, knowing diffusion coefficients is vital and gives useful information about the physical properties of diffusive particles and the environment. In many cases a diffusive particle is not a sphere and has rotation during its movements. In these cases information about a particle''s trajectory both in lab and body frame would be useful. Also some statistical analysis is needed to obtain more information about a particle''s motion. Solution method: This program tries to gather all required tools to analyse raw data from the Brownian motion of a diffusing particle. Ability to switch between different methods of calculation of mean square displacement to find diffusion coefficients depends on the correlations between data points. There are three methods in the program: time average, ensemble average and their combinations. A linear fit is done to measure Diffusion Coefficient (D), the weight and fraction of data points is controllable. Given physical properties of the system, the program can calculates D theoretically for some basic geometrical shapes; sphere, spheroid and cylinder. In the case of non-spherical particles if data of rotation is available, the code can calculate trajectory and diffusion also in body frame. There are more statistical tools available in the program, such as histogram and autocorrelation function to obtain more information e.g. relaxation time to ideal diffusion motion. Code uses log–log diagram of mean square displacement (MSD) to calculate the amount of deviation from normal diffusion to sub- or super-diffusion. Running time: It is dependent on the input data, but for typical data in the order of mega bytes, it would take tens of minutes. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
8. Tuning fluorophore excitation in a total-internal-reflection-fluorescence microscopy.
- Author
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Sheykhi E, Sajad B, Tavaddod S, Naderi-Manesh H, and Roostaiei N
- Abstract
In a total-internal-reflection-fluorescence-microscopy method, there is anisotropy in the polarized evanescent wave. Since the evanescent wave is used as an excitation field, the mentioned anisotropy is a disadvantage in using the total-internal-reflection-fluorescence-microscopy technique. Therefore, by theoretical and analytical approaches, and based on the Fresnel coefficients, the effect of three dielectrics media on the anisotropy of the evanescent wave is investigated. Following that, a proper combination of the cover glass, oil immersion, and prism for both living and non-living samples is suggested that not only enhances the intensity of the evanescent wave, but also and importantly, decreases the essential anisotropy of the evanescent wave.
- Published
- 2019
- Full Text
- View/download PDF
9. Evidence of Multi-Domain Morphological Structures in Living Escherichia coli.
- Author
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Tavaddod S and Naderi-Manesh H
- Subjects
- Cell Shape, Cell Wall ultrastructure, Image Processing, Computer-Assisted, Microscopy, Escherichia coli cytology
- Abstract
A combination of light-microscopy and image processing was used to elaborate on the fluctuation in the width of the cylindrical part of Escherichia coli at sub-pixel-resolution, and under in vivo conditions. The mean-squared-width-difference along the axial direction of the cylindrical part of a number of bacteria was measured. The results reveal that the cylindrical part of Escherichia coli is composed of multi-domain morphological structures. The length of the domains starts at 150 nm in newborn cells, and linearly increases in length up to 300 nm in aged cells. The fluctuation in the local-cell-widths in each domain is less than the fluctuation of local-cell-widths between different domains. Local cell width correlations along the cell body occur on a length scale of less than 50 nm. This finding could be associated with the flexibility of the cell envelope in the radial versus longitudinal directions.
- Published
- 2017
- Full Text
- View/download PDF
10. D, L-Sulforaphane Loaded Fe3O4@ Gold Core Shell Nanoparticles: A Potential Sulforaphane Delivery System.
- Author
-
Kheiri Manjili H, Ma'mani L, Tavaddod S, Mashhadikhan M, Shafiee A, and Naderi-Manesh H
- Subjects
- Apoptosis, Drug Delivery Systems, Humans, MCF-7 Cells, Metal Nanoparticles chemistry, Microscopy, Electron, Spectrometry, X-Ray Emission, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Sulfoxides, Ferric Compounds administration & dosage, Gold chemistry, Isothiocyanates administration & dosage, Metal Nanoparticles administration & dosage
- Abstract
A novel design of gold-coated iron oxide nanoparticles was fabricated as a potential delivery system to improve the efficiency and stability of d, l-sulforaphane as an anticancer drug. To this purpose, the surface of gold-coated iron oxide nanoparticles was modified for sulforaphane delivery via furnishing its surface with thiolated polyethylene glycol-folic acid and thiolated polyethylene glycol-FITC. The synthesized nanoparticles were characterized by different techniques such as FTIR, energy dispersive X-ray spectroscopy, UV-visible spectroscopy, scanning and transmission electron microscopy. The average diameters of the synthesized nanoparticles before and after sulforaphane loading were obtained ∼ 33 nm and ∼ 38 nm, respectively, when ∼ 2.8 mmol/g of sulforaphane was loaded. The result of cell viability assay which was confirmed by apoptosis assay on the human breast cancer cells (MCF-7 line) as a model of in vitro-cancerous cells, proved that the bare nanoparticles showed little inherent cytotoxicity, whereas the sulforaphane-loaded nanoparticles were cytotoxic. The expression rate of the anti-apoptotic genes (bcl-2 and bcl-xL), and the pro-apoptotic genes (bax and bak) were quantified, and it was found that the expression rate of bcl-2 and bcl-xL genes significantly were decreased when MCF-7 cells were incubated by sulforaphane-loaded nanoparticles. The sulforaphane-loaded into the designed gold-coated iron oxide nanoparticles, acceptably induced apoptosis in MCF-7 cells.
- Published
- 2016
- Full Text
- View/download PDF
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