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Today, transformerless inverters (TIs) are widely applicable in different solar photovoltaic (PV) grid-connected applications owing to their promising features, such as higher efficiency and power density. However, high-frequency common-mode voltage (CMV) in these topologies can result in high leakage current, electromagnetic interference, and lack of safety, reducing the whole system’s reliability. To resolve the problems associated with TIs, this paper proposes a novel hybrid switched capacitor (SC)-based common-ground (CG) transformerless inverter (TI) topology, which can be applied in grid-connected photovoltaic (PV) applications. The boost inductor is integrated to achieve continuous input current and dynamic voltage gain. In addition, the proposed circuit comprises nine switches and two SCs with a single input DC source. It can generate five-level AC voltage with voltage boosting within a single-stage DC–AC power conversion. The working principles of the proposed topology, circuit description, and control technique are presented. Furthermore, the proposed inverter is comprehensively compared with other five-level TIs to show its superiority. Finally, a laboratory prototype is developed and tested to validate the practical viability of the proposed configuration.

A single-phase common-ground five-level (5L) inverter with a dynamic voltage conversion gain and capability of operating in a wide input voltage range and a single-stage energy conversion configuration is presented in this article. The proposed topology requires nine active power switches and is comprised of an integrated switched-boost (SB) module connected in series to a switched-flying-capacitor (SFC) cell. Two self-balanced capacitors with a single boost inductor in the integrated SB module are employed to generate a 5L output voltage waveform with a dynamic voltage conversion gain. The current stress profile of all the active and passive elements is kept within a permissible input current range. By adopting an extra diode-capacitor-inductor network into the integrated SB module and with the utilization of the same SFC cell, the proposed topology is extended to achieve a quadratic voltage conversion gain while retaining the quality of ac voltage waveform. Theoretical analysis, closed-loop control/modulation principles, design guidance, comparative study, and relevant experimental results obtained from a 1.5-kW laboratory-built prototype are presented to ascertain the operation and feasibility of the proposed system.

In this article, we present a family of multilevel converters with the single-stage dynamic voltage-boosting feature, reduced number of circuit components, modular structure, bidirectional operation, continuous input current, and acceptable overall efficiency. The proposed structure is based on a three-level single-stage boost integrated inverter with an embedded quasi-H-bridge (QHB) cell. It is comprised of five unidirectional power switches and a floating capacitor. By differential connection of two or three QHB cells and with the aim of a single inductor/input dc source, several derived topologies for both the single and three-phase applications with different multilevel output voltage performances have been achieved. The aforementioned advantages make this converter a suitable candidate for renewable energy applications. Theoretical analysis, design consideration, comparative study, and several experimental results for a 3-kW laboratory-built system are presented to validate the effectiveness and feasibility of this proposal.

Multilevel inverters (MLIs) with switched-capacitor (SC) units have been a widely rehearsed research topic in power electronics since the last decade. Inductorless/transformerless operation with voltage-boosting feature and inherent capacitor self-voltage balancing performance with a reduced electromagnetic interference make the SC-MLI an attractive converter over the other available counterparts for various applications. There have been many developed SC-MLI structures recently put forward, where different basic switching techniques are used to generate multiple (discrete) output voltage levels. In general, the priority of the topological development is motivated by the number of output voltage levels, overall voltage gain, and full dc-link voltage utilization, while reducing the component counts and stress on devices for better efficiency and power density. To facilitate the direction of future research in SC-MLIs, this article presents a comprehensive review, critical analysis, and categorization of the existing topologies. Common fundamental units are generalized and summarized with their merits and demerits. Ultimately, major challenges and research directions are outlined leading to the future technology roadmap for more practical applications.

This paper proposes a universal converter that is capable of operating in three modes for generating positive dc voltage, negative dc voltage, and sinusoidal ac voltage. By controlling the duty-cycle of two half-bridges, an inductor is operated at a high frequency to control the voltage across two film capacitors that constitute a dual-buck-boost converter. Two additional half-bridges operating at a fixed state or line frequency are used to select the mode of operation. Compared to the latest universal converter in the recent literature, the proposed topology has the same switch count while reducing the number of conducting switches for inductor current and reducing the number of switches operating at high frequency. The operation of the proposed dual-buck-boost dc–dc/ac universal converter is analyzed. Experimental results are presented for validation. The power conversion efficiency of the 100 W experimental prototype modeled in PLECS is approximately 98%.

Voltage source multilevel inverters with reduced leakage current, single-stage voltage step-up feature, compact design, and an efficient performance are a promising technology for high-frequency ac (HFac) microgrids feeding through renewable energy sources. This article proposes a novel single-source common-grounded (CG) step-up nine-level (9L) inverter, which can be applied in HFac microgrid applications. The proposed CG-based boost inverter is comprised of only nine switches (9S) and three self-balanced capacitors. Using the switched-capacitor (SC) technique, a double voltage boosting feature within a single power processing stage is achieved, while the leakage current concern is eliminated due to a CG-based configuration between the input dc source and the null of the grid. With the help of an LC input filter, the input current profile is free from large discontinuous inrush spikes. The working principles of the proposed 9L9S-CGSC inverter are discussed in this article. The modulation and closed-loop control strategy, as well as a comparative study, are presented. Finally, the open and closed-loop grid-tied performances of the proposed topology are evaluated by both simulation and experimental results obtained from a 1.2-kW laboratory-built prototype.

This paper presents a common-ground five-level transformerless inverter for solar photovoltaic (PV) applications. In the proposed inverter, the grid neutral line is directly connected to the negative pole of the dc bus eliminating the common-mode leakage current which is a critical problem in grid-connected PV systems. Moreover, the proposed inverter has only four power switches, making the first five-level common-ground topology with less than six switches found in the recent literature. In addition to low switch count, only two switches are operating in high-frequency. The operation modes, the design guidelines as well as a modulation strategy are presented. Simulation and experimental results are obtained to demonstrate the performance of the proposed inverter.

The circuit performance of conventional active neutral point-clamped (ANPC) inverter is widely accepted in many renewable energy-based applications like photovoltaic (PV) or electric vehicle grid-connected systems. This is mainly because of its excellent characteristics in terms of voltage/current stress profile of the switches, bidirectional power flow capability, and efficient operation. Nonetheless, due to its half-dc link voltage utilization in the ac output voltage, another power processing stage with additional active and passive elements is required to make its output voltage compatible with the grid when low and wide varying input dc source is available. In this paper, a novel ANPC-based five-level (ANPC5L) inverter with a single-stage boost-integrated circuit design is presented. The proposed topology is able to make the peak output voltage of the conventional ANPC5L inverter followed by a front-end bidirectional boost converter double using the same number of power switches but with less total standing voltage across semiconductors. The working principles of the proposed topology is discussed. Experimental results obtained from 1.3 kW laboratory-built prototype under the grid-connected condition are also given to support the discussion.

Transfomerless grid-connected inverters with common-grounded circuit architectures and single-stage dy-namic voltage boosting gain are promising candidates for PV energy conversion applications. In this work, a new five-level (5L) variant of this category of inverters is introduced. Key features of the presented inverter are the reduced current stress profile, modularity, uniform peak voltage stress across the switches, higher power handling capability, and bi-directional power flow operation. The proposed topology is comprised of two input inductors, two capacitors, and ten power switches. Through a modular design with a phase-shifted SPWM technique, the injected grid current can be shared among the modules, while the size of the grid-interface filters can be reduced. The working principle of the converter is discussed, and some simulation and experimental results are presented to validate its feasibility.

Transformerless (TL) grid-connected photovoltaic (PV) inverters with a common-ground (CG) circuit architecture exhibit some excellent features in removing the leakage current concern and improving the overall efficiency. However, the ability to cope with a wide range of input voltage changes while maintaining the output voltage in a single power conversion stage is a key technological challenge. Considering this, the article at hand proposes a novel dual-mode switched-capacitor five-level (DMSC5L)-TL inverter with a CG feature connected to the grid. The proposed topology is comprised of a single dc source and power diode, three capacitors, four unidirectional, and three bidirectional power switches. Based on the series-parallel switching conversion of the involved switches, the proposed DMSC5L-TL inverter can generate five distinctive output voltage levels during both the boost and buck operation modes with a self-voltage balancing operation for the involved capacitors. A simple dead-beat continuous current controller (DB3C) modulation technique is also used to handle both the active and reactive power exchange while ensuring a fixed switching frequency operation. The proposed circuit description with its DB3C details, the design guidelines with a comparative study, and some experimental results are also given to show the feasibility of the proposed solution for the practical applications.

Single-source inverters with integrated switched-boost (SB) module and single-stage energy power conversion architecture have become a popular solution in many power electronics applications. This paper presents a novel topology of such types of inverters, which offers a modular design with a dynamic voltage boosting feature over a wide range of input voltages. The basic configuration of the proposed topology is able to generate three output voltage levels. It is comprised of an integrated SB module and a Quasi-H-Bridge (QHB) cell. The QHB cell consists of a self-balanced floating capacitor and four power switches, and the SB cell consists of a boost inductor and a single power switch. A five-level boosted output voltage can be achieved through the differential connection of two identical QHB cells utilizing a single SB module. The circuit description of the proposed topology, a comparative study, and the simulation and measurement results are given to verify the feasibility of this proposal.

This article presents a novel cascaded multilevel inverter as an alternative to the cascaded H-bridge (CHB). By adopting two H-bridges integrated with two inductors and two power switches, the proposed topology is able to generate a five-level voltage with merely a single dc source. Voltage-boosting capability is guaranteed despite its single-stage operation. A corresponding modulation technique is also established to charge the boost inductors with a constant duty-cycle while simultaneously generating a five-level ac voltage. Therefore, the proposed topology outperforms the CHB in reducing the isolated dc source count, enhancing the voltage gain, and lowering the dc source voltage magnitude. In addition, the modularity is preserved, where the proposed topology can be extended by cascading multiple modules. The operation and feasibility of the proposed cascaded multilevel inverter is analyzed and validated through simulation and experimental results.

This paper explores the feasibility of multilevel dual-active bridge-inverter (DABMI) applications for grid-connected applications of a modern Model of Predictive Direct Power Control (MPDPC) based on the conservative power theory (CPT). In the case of unbalanced grid voltages, the objective of the study is to promote continued active and reactive energy in MPDPC without reducing efficiency such as transient response and current harmonics. The nature of the instantaneous p-q theory permits only one out of three control targets to be fulfilled. The proposed control approached directly regulates the instantaneous active and reactive power to achieve three particular control objectives namely sinusoidal and symmetrical grid current, cancelling twice of fundamental grid frequency reactive power ripples, and removing twice grid frequency active power ripple. The techniques of complicated Grid part sequence extraction are unnecessary and improved at no extra expense, as is the case with current MPDPC fault-tolerant approaches. The instantaneous power at the next sampling instant is predicted with the newly developed discrete-time model. Each possible switching state will then be evaluated in the cost function defined until the optimal state which lead to the minimum power errors is determined. In MATLAB/Simulink simulation, the proposed CPT-based MPDPC measures reliability and performance at balanced and unbalanced grid voltages then compared with the conventional and existing MPDPC The proposed method manages to achieve all of three control targets which generates sinusoidal grid currents and attenuates active and reactive power ripple of twice the grid frequency exactly at the same time without losing its critical efficiency including transient reaction and current harmonics.

To answer the increasing need for detecting and validating protein biomarkers in clinical specimens, proteomic techniques are required that support the fast, reproducible and quantitative analysis of large clinical sample cohorts. Targeted mass spectrometry techniques, specifically SRM, PRM and the massively parallel SWATH/DIA technique have emerged as a powerful method for biomarker research. For optimal performance, they require prior knowledge about the fragment ion spectra of targeted peptides. In this report, we describe a mass spectrometric (MS) pipeline and spectral resource to support data-independent acquisition (DIA) and parallel reaction monitoring (PRM) based biomarker studies. To build the spectral resource we integrated common open-source MS computational tools to assemble an open source computational workflow based on Docker. It was then applied to generate a comprehensive DIA pan-human library (DPHL) from 1,096 data dependent acquisition (DDA) MS raw files, and it comprises 242,476 unique peptide sequences from 14,782 protein groups and 10,943 SwissProt-annotated proteins expressed in 16 types of cancer samples. In particular, tissue specimens from patients with prostate cancer, cervical cancer, colorectal cancer, hepatocellular carcinoma, gastric cancer, lung adenocarcinoma, squamous cell lung carcinoma, diseased thyroid, glioblastoma multiforme, sarcoma and diffuse large B-cell lymphoma (DLBCL), as well as plasma samples from a range of hematologic malignancies were collected from multiple clinics in China, the Netherlands and Singapore and included in the resource. This extensive spectral resource was then applied to a prostate cancer cohort of 17 patients, consisting of 8 patients with prostate cancer (PCa) and 9 with benign prostate hyperplasia (BPH), respectively. Data analysis of DIA data from these samples identified differential expressions of FASN, TPP1 and SPON2 in prostate tumors. Thereafter, PRM validation was applied to a larger PCa cohort of 57 patients and the differential expressions of FASN, TPP1 and SPON2 in prostate tumors were validated. As a second application, the DPHL spectral resource was applied to a patient cohort consisting of samples from 19 DLBCL patients and 18 healthy individuals. Differential expressions of CRP, CD44 and SAA1 between DLBCL cases and healthy controls were detected by DIA-MS and confirmed by PRM. These data demonstrate that the DPHL supported that DIA-PRM MS pipeline enables robust protein biomarker discoveries.

The conventional active neutral-point-clamped (ANPC) inverter inherits excellent high-frequency common mode voltage (CMV) mitigation ability but with a restricted voltage gain. This letter proposes a novel five-level ANPC inverter that is able to accomplish voltage-boosting within a single-stage dc–ac power conversion. A common ground in the proposed topology not only mitigates the high-frequency CMV but also enhances the dc-link voltage utilization. The proposed topology exhibits lower voltage stresses and higher compactness when compared with a conventional two-stage five-level ANPC inverter. The proposed inverter saves three power switches and one capacitor while enhancing the overall efficiency. Simulations and experimental tests have validated the overall operation, efficacy, and practicality of the proposed ANPC inverter.

Genetically modified FVIII‐expressing autologous bone marrow‐derived mesenchymal stromal cells (BMSCs) could cure haemophilia A. However, culture‐expanded BMSCs engraft poorly in extramedullary sites. Here, we compared the intramedullary cavity, skeletal muscle, subcutaneous tissue and systemic circulation as tissue microenvironments that could support durable engraftment of FVIII‐secreting BMSC in vivo. A zinc finger nuclease integrated human FVIII transgene into PPP1R12C (intron 1) of culture‐expanded primary canine BMSCs. FVIII‐secretory capacity of implanted BMSCs in each dog was expressed as an individualized therapy index (number of viable BMSCs implanted × FVIII activity secreted/million BMSCs/24 hours). Plasma samples before and after implantation were assayed for transgenic FVIII protein using an anti‐human FVIII antibody having negligible cross‐reactivity with canine FVIII. Plasma transgenic FVIII persisted for at least 48 weeks after implantation in the intramedullary cavity. Transgenic FVIII protein levels were low after intramuscular implantation and undetectable after both intravenous infusion and subcutaneous implantation. All plasma samples were negative for anti‐human FVIII antibodies. Plasma concentrations and durability of transgenic FVIII secretion showed no correlation with the therapy index. Thus, the implantation site microenvironment is crucial. The intramedullary microenvironment, but not extramedullary tissues, supported durable engraftment of genetically modified autologous FVIII‐secreting BMSCs.

Chromosomal rearrangements are common in cancer. More than 50% occur in common fragile sites and disrupt tumor suppressors. However, such rearrangements are not known in gastric cancer. Here we report recurrent 18q2 breakpoints in 6 of 17 gastric cancer cell lines. The rearranged chromosome 18, t(9;18), in MKN7 cells was flow sorted and identified by reverse chromosome painting. High-resolution tiling array hybridization mapped breakpoints to DOK6 (docking protein 6) intron 4 in FRA18C (18q22.2) and an intergenic region in 9q22.2. The same rearrangement was detected by FISH in 22% of 99 primary gastric cancers. Intron 4 truncation was associated with reduced DOK6 transcription. Analysis of The Cancer Genome Atlas stomach adenocarcinoma cohort showed significant correlation of DOK6 expression with histological and molecular phenotypes. Multiple oncogenic signaling pathways (gastrin-CREB, NGF-neurotrophin, PDGF, EGFR, ERK, ERBB4, FGFR1, RAS, VEGFR2 and RAF/MAP kinase) known to be active in aggressive gastric cancers were strikingly diminished in gastric cancers with low DOK6 expression. Median survival of patients with low DOK6-expressing tumors was 2100 days compared with 533 days in patients with high DOK6-expressing tumors (log-rank P = 0.0027). The level of DOK6 expression in tumors predicted patient survival independent of TNM stage. These findings point to new functions of human DOK6 as an adaptor that interacts with diverse molecular components of signaling pathways. Our data suggest that DOK6 expression is an integrated biomarker of multiple oncogenic signals in gastric cancer and identify FRA18C as a new cancer-associated fragile site. Gastric tumors often harbor a chromosome abnormality that disrupts a key signaling gene, resulting in less aggressive cancers. Oi Lian Kon from the National Cancer Centre Singapore and colleagues searched for chromosomal rearrangements in 17 gastric cancer cell lines. The researchers discovered a recurrent breakpoint in six of the cell lines that mapped to a gene called DOK6, which encodes a protein that provides a docking platform for multiple signaling molecules. They also found the abnormality in 22 of 99 tissue samples taken from gastric cancer patients. This defect led to lower expression of DOK6 and, in turn, less active oncogenic signaling pathways. Patients with low DOK6-expressing gastric cancers lived longer on average than those with high DOK6-expressing tumors. The findings point to DOK6 levels as a potential drug target and diagnostic biomarker.

This paper presents a comparative study of the proportional-integral-based (PI-based) synchronous current control strategy with derivative-feedback-based active damping and the finite-control-set model-predictive-control-based (FCS-MPC-based) synchronous current control strategy with cost-function-based active damping. For a fair comparison, the sensor requirement and the average switching frequency of FCS-MPC are made equivalent to that of the pulse-width-modulation-based counterpart through internal model estimation and control sampling frequency adjustment. The comparative study considers gain/weighting-factor tuning, delay compensation, switching harmonics, and active damping performance at the critical frequency operating point. The overall performance of both schemes is validated through the same experimental setup and test scenarios. The results conclude that the emerging FCS-MPC has the potential to produce similar results as the classical PI-based counterpart while carrying some practical features. These include being intuitive in active damping design and tuning, guaranteeing fast dynamics, and being sufficiently robust to grid impedance shifting. These findings essentially justify the potential of model predictive control being a viable alternative for this area of application.

The single-phase split-source inverter is an emerging and attractive topology for boost dc-ac power conversion system. Such an inverter features high compactness, however, at the expense of high-frequency commutations across diodes. The corresponding hybrid PWM also confines the voltage harmonics to concentrate around the switching frequency and its multiples. This paper proposes a simplified split-source inverter which is realized by inserting only one power switch into an H-bridge. While generating the ac output, the newly developed PWM strategy ensures the inductor charged with constant duty-cycle. When compared to the existing split-source inverters, it offers the added benefits of reduced switch count, enhanced voltage boosting gain, reduced output filter requirement, and enhanced power efficiency. Comprehensive steady-state analysis is discussed while simulation and experimental results are subsequently presented to prove the validity of the proposed topology and the PWM strategy.

This paper presents a model predictive control (MPC) for off-board plug-in electric vehicle (PEV) chargers with photovoltaic (PV) integration using two-level four-leg inverter topology. The PEV charger is controlled by a unified controller that incorporates direct power and current MPC to dynamically control decoupled active-reactive power flow in a smart grid environment as well as to control PEV battery charging and discharging reliably. PV power generation with maximum power tracking is seamlessly integrated with the power flow control to provide additional power generation. Fast dynamic response and good steady-state performance under all power flow modes and various environmental conditions are evaluated and analyzed. From the results obtained, the charger demonstrates less than 1.5% total harmonic distortion as well as low active and reactive power ripple of less than 7% and 8% respectively on the grid for all power flow modes. The PEV battery also experiences a low charging and discharging current ripple of less than 2.5%. Therefore, the results indicate the successful implementation of the proposed charger and its control for PV integrated off-board PEV chargers.

A two-stage switched-capacitor based multilevel inverter possesses a drawback such that switches in the second stage (i.e., H-bridge) endure higher voltage stress. To resolve this problem, this letter proposes a single-stage switched-capacitor module (S3CM) topology for cascaded multilevel inverter, which ensures the peak inverse voltage across all the switches within the dc source voltage. A total of nine voltage levels can be generated with only one dc source and two incorporated capacitors. Hence, the number of isolated dc sources is significantly reduced compared to a cascaded H-bridge. In addition, voltage boosting gain of two is achieved. A comparative analysis of the recent topology reveals that the proposed S3CM topology achieves switch count reduction. The operation of the proposed topology is validated through circuit analysis followed by simulation and experimental results of a single-module (9-level) prototype.

This paper presents a boost-multilevel inverter design with integrated battery energy storage system for standalone application. The inverter consists of modular switched-battery cells and a full-bridge. It is multifunctional and has two modes of operation: 1) the charging mode, which charges the battery bank and 2) the inverter mode, which supplies ac power to the load. This inverter topology requires significantly less power switches compared to conventional topology such as cascaded H-bridge multilevel inverter, leading to reduced size/cost and improved reliability. To selectively eliminate low-order harmonics and control the desired fundamental component, nonlinear system equations are represented in fitness function through the manipulation of modulation index and the genetic algorithm (GA) is employed to find the optimum switching angles. A seven-level inverter prototype is implemented and experimental results are provided to verify the feasibility of the proposed inverter design.

Gastric cancer remains highly fatal due to a dearth of diagnostic biomarkers for early stage disease and molecular targets for therapy. Plasma membrane proteins, including cluster of differentiation (CD) proteins and receptor tyrosine kinases (RTKs), are a rich reservoir of biomarkers. Recognizing that interrogating plasma membrane proteins individually overlooks extensive interactions among them, we have systematically investigated the membrane proteomes and transcriptomes of six gastric cancer cell lines. Our data revealed aberrantly high expression of proteins whose functions accurately reflect the clinical phenotype of gastric cancer, and prioritized critical RTKs and CD proteins in gastric cancer. Expression of selected surface proteins was confirmed by flow cytometry and immunostaining of clinical gastric cancer tissues. Close to 90% of the gastric cancer tissues in a cohort showed up-regulation of at least one of four proteins, that is, MET, EPHA2, FGFR2, and CD104/ITGB4. All intestinal type gastric cancer tumors in this cohort overexpressed at least one of a panel of three proteins, MET, FGFR2, and EPHA2. This study reports the first quantitative global landscape of the surface proteome of gastric cancer cells and provides a shortlist of gastric cancer biomarkers.

Biosafety and efficacy considerations that impede clinical application of gene therapy could be addressed by nonviral ex vivo cell therapy, utilizing transgenic cells that have been comprehensively pre-evaluated for genotoxic potential and transgene expression. We evaluated the genotoxic potential of phiC31 bacteriophage integrase-mediated transgene integration in cord-lining epithelial cells (CLECs) readily cultured from the outer membrane of human umbilical cords, by sequencing and mapping integration sites, spectral karyotyping, high-resolution genome copy number, transcriptome, and transgene copy number analyses and in vivo tumorigenicity. Of 44 independent integration events