16 results on '"Suttner K"'
Search Results
2. Actuator development for the Instrument Pointing System (IPS)
- Author
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Suttner, K
- Subjects
Instrumentation And Photography - Abstract
The mechanisms of the instrument pointing system (IPS) are described. Particular emphasis is placed on the actuators which are necessary for operating the IPS. The actuators are described as follows: (1) two linear actuators that clamp the gimbals down during ascent and descent; (2) two linear actuators that attach the payload to the IPS during the mission, and release it into the payload clamps; (3) one rotational actuator that opens and closes the payload clamps; and (4) three identical drive units that represent the three orthogonal gimbal axes and are the prime movers for pointing. Design features, manufacturing problems, test performance, and results are presented.
- Published
- 1984
3. GEOS-20 m cable boom mechanism
- Author
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Schmidt, B. K and Suttner, K
- Subjects
Mechanical Engineering - Abstract
The GEOS cable boom mechanism allows the controlled deployment of a 20 m long cable in a centrifugal force field. In launch configuration the flat cable is reeled on a 240 mm diameter drum. The electrical connection between the rotating drum and the stationary housing is accomplished via a flexlead positioned inside the drum. Active motion control of this drum is achieved by a self locking worm gear, driven by a stepper motor. The deployment length of the cable is monitored by an optical length indicator, sensing black bars engraved on the cable surface.
- Published
- 1977
4. The GEOS-20 m Cable Boom Mechanism
- Author
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Schmidt, G. K and Suttner, K
- Subjects
Launch Vehicles And Space Vehicles - Abstract
The GEOS Cable Boom Mechanism which allows the controlled deployment of a 20 m long cable in a centrifugal force field is described. In launch configuration the flat cable is reeled on a 240 mm diameter drum. The electrical connection between the rotating drum and the stationary housing is accomplished via a flexlead positioned inside the drum. Active motion control of this drum is achieved by a self locking worm gear, driven by a stepper motor. The deployment length of the cable is monitored by an optical length indicator, sensing black bars engraved on the cable surface.
- Published
- 1977
5. Characterisation of Gene Variations in the Transcription Factors T-bet and HLX1 and their Functional Role in the Development of Asthma
- Author
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Suttner, K., Rosenstiel, P., Depner, M., Ruoss, I., Peters, W., Klopp, N., Illig, T., Vogelberg, C., Weiland, S.K., von Mutius, E., and Kabesch, M.
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- 2008
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6. Early IL-10 producing B-cells and coinciding Th/Tr17 shifts during three year grass-pollen AIT.
- Author
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Zissler UM, Jakwerth CA, Guerth FM, Pechtold L, Aguilar-Pimentel JA, Dietz K, Suttner K, Piontek G, Haller B, Hajdu Z, Schiemann M, Schmidt-Weber CB, and Chaker AM
- Subjects
- B-Lymphocytes immunology, Cells, Cultured, Humans, Poaceae immunology, B-Lymphocytes metabolism, Desensitization, Immunologic, Interleukin-10 metabolism, Pollen immunology, Rhinitis, Allergic, Seasonal therapy, T-Lymphocytes immunology
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- 2018
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7. Doublesex and mab-3 related transcription factor 1 (DMRT1) is a sex-specific genetic determinant of childhood-onset asthma and is expressed in testis and macrophages.
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Schieck M, Schouten JP, Michel S, Suttner K, Toncheva AA, Gaertner VD, Illig T, Lipinski S, Franke A, Klintschar M, Kalayci O, Sahiner UM, Birben E, Melén E, Pershagen G, Freidin MB, Ogorodova LM, Granell R, Henderson J, Brunekreef B, Smit HA, Vogelberg C, von Berg A, Bufe A, Heinzmann A, Laub O, Rietschel E, Simma B, Genuneit J, Jonigk D, Postma DS, Koppelman GH, Vonk JM, Timens W, Boezen HM, and Kabesch M
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- Age of Onset, Alleles, Asthma immunology, Binding Sites, Child, Chromosome Mapping, Female, Genetic Loci, Genome-Wide Association Study, Humans, Immunohistochemistry, Linkage Disequilibrium, Macrophages immunology, Male, Odds Ratio, Organ Specificity genetics, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Sex Factors, Transcription Factors metabolism, Asthma genetics, Gene Expression, Genetic Predisposition to Disease, Macrophages metabolism, Testis metabolism, Transcription Factors genetics
- Abstract
Background: Asthma is a disease affecting more boys than girls in childhood and more women than men in adulthood. The mechanisms behind these sex-specific differences are not yet understood., Objective: We analyzed whether and how genetic factors contribute to sex-specific predisposition to childhood-onset asthma., Methods: Interactions between sex and polymorphisms on childhood asthma risk were evaluated in the Multicentre Asthma Genetics in Childhood Study (MAGICS)/Phase II International Study of Asthma and Allergies in Childhood (ISAAC II) population on a genome-wide level, and findings were validated in independent populations. Genetic fine mapping of sex-specific asthma association signals was performed, and putatively causal polymorphisms were characterized in vitro by using electrophoretic mobility shift and luciferase activity assays. Gene and protein expression of the identified gene doublesex and mab-3 related transcription factor 1 (DMRT1) were measured in different human tissues by using quantitative real-time PCR and immunohistochemistry., Results: Polymorphisms in the testis-associated gene DMRT1 displayed interactions with sex on asthma status in a population of primarily clinically defined asthmatic children and nonasthmatic control subjects (lowest P = 5.21 × 10(-6)). Replication of this interaction was successful in 2 childhood populations clinically assessed for asthma but showed heterogeneous results in other population-based samples. Polymorphism rs3812523 located in the putative DMRT1 promoter was associated with allele-specific changes in transcription factor binding and promoter activity in vitro. DMRT1 expression was observed not only in the testis but also in lung macrophages., Conclusion: DMRT1 might influence sex-specific patterns of childhood asthma, and its expression in testis tissue and lung macrophages suggests a potential involvement in hormone or immune cell regulation., (Copyright © 2016 American Academy of Allergy, Asthma & Immunology. All rights reserved.)
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- 2016
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8. A Combined Omics Approach to Generate the Surface Atlas of Human Naive CD4+ T Cells during Early T-Cell Receptor Activation.
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Graessel A, Hauck SM, von Toerne C, Kloppmann E, Goldberg T, Koppensteiner H, Schindler M, Knapp B, Krause L, Dietz K, Schmidt-Weber CB, and Suttner K
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- Cell Membrane metabolism, Cluster Analysis, Computer Simulation, Flow Cytometry, Gene Expression Profiling, Gene Ontology, Glycoproteins metabolism, Humans, Proteome metabolism, Reproducibility of Results, Transcriptome genetics, CD4-Positive T-Lymphocytes metabolism, Lymphocyte Activation immunology, Proteomics methods, Receptors, Antigen, T-Cell metabolism
- Abstract
Naive CD4(+) T cells are the common precursors of multiple effector and memory T-cell subsets and possess a high plasticity in terms of differentiation potential. This stem-cell-like character is important for cell therapies aiming at regeneration of specific immunity. Cell surface proteins are crucial for recognition and response to signals mediated by other cells or environmental changes. Knowledge of cell surface proteins of human naive CD4(+) T cells and their changes during the early phase of T-cell activation is urgently needed for a guided differentiation of naive T cells and may support the selection of pluripotent cells for cell therapy. Periodate oxidation and aniline-catalyzed oxime ligation technology was applied with subsequent quantitative liquid chromatography-tandem MS to generate a data set describing the surface proteome of primary human naive CD4(+) T cells and to monitor dynamic changes during the early phase of activation. This led to the identification of 173 N-glycosylated surface proteins. To independently confirm the proteomic data set and to analyze the cell surface by an alternative technique a systematic phenotypic expression analysis of surface antigens via flow cytometry was performed. This screening expanded the previous data set, resulting in 229 surface proteins, which were expressed on naive unstimulated and activated CD4(+) T cells. Furthermore, we generated a surface expression atlas based on transcriptome data, experimental annotation, and predicted subcellular localization, and correlated the proteomics result with this transcriptional data set. This extensive surface atlas provides an overall naive CD4(+) T cell surface resource and will enable future studies aiming at a deeper understanding of mechanisms of T-cell biology allowing the identification of novel immune targets usable for the development of therapeutic treatments., (© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.)
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- 2015
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9. Genetic variation in TH17 pathway genes, childhood asthma, and total serum IgE levels.
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Schieck M, Michel S, Suttner K, Illig T, Zeilinger S, Franke A, Vogelberg C, von Berg A, Bufe A, Heinzmann A, Laub O, Rietschel E, Simma B, Frischer T, Genuneit J, Kerzel S, and Kabesch M
- Subjects
- Genetic Variation, Humans, Interleukin-17 genetics, Receptors, Interleukin genetics, Asthma genetics, Asthma immunology, Immunoglobulin E blood, Polymorphism, Single Nucleotide, Th17 Cells immunology
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- 2014
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10. Genetic variants in Protocadherin-1, bronchial hyper-responsiveness, and asthma subphenotypes in German children.
- Author
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Toncheva AA, Suttner K, Michel S, Klopp N, Illig T, Balschun T, Vogelberg C, von Berg A, Bufe A, Heinzmann A, Laub O, Rietschel E, Simma B, Frischer T, Genuneit J, von Mutius E, and Kabesch M
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- Child, Chromosomes, Human, Pair 5 genetics, Cytokines genetics, Disease Progression, Female, Genetic Association Studies, Germany, High-Throughput Nucleotide Sequencing, Humans, Linkage Disequilibrium, Male, Phenotype, Polymorphism, Genetic, Protocadherins, Asthma physiopathology, Bronchial Hyperreactivity genetics, Cadherins genetics
- Abstract
Background: Recently, Protocadherin-1 (PCDH1) was reported as a novel susceptibility gene for bronchial hyper-responsiveness (BHR) and asthma. PCDH1 is located on chromosome 5q31-33, in the vicinity of several known candidate genes for asthma and allergy. To exclude that the associations observed for PCDH1 originate from the nearby cytokine cluster, an extensive linkage disequilibrium (LD) analysis was performed. Effects of polymorphisms in PCDH1 on asthma, BHR, and related phenotypes were studied comprehensively., Methods: Genotype information was acquired from Illumina HumanHap300Chip genotyping, MALDI-TOF MS genotyping, and imputation. LD was assessed by Haploview 4.2 software. Associations were investigated in a population of 1454 individuals (763 asthmatics) from two German study populations [MAGICS and International Study of Asthma and Allergies in Childhood phase II (ISAAC II)] using logistic regression to model additive effects., Results: No relevant LD between PCDH1 tagging polymorphisms and 98 single nucleotide polymorphisms within the cytokine cluster was detected. While BHR was not associated with PCDH1 polymorphisms, significant associations with subphenotypes of asthma were observed., Conclusion: Protocadherin-1 polymorphisms may specifically affect the development of non-atopic asthma in children. Functional studies are needed to further investigate the role of PCDH1 in BHR and asthma development., (© 2012 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.)
- Published
- 2012
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11. TBX21 and HLX1 polymorphisms influence cytokine secretion at birth.
- Author
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Casaca VI, Illi S, Suttner K, Schleich I, Ballenberger N, Klucker E, Turan E, von Mutius E, Kabesch M, and Schaub B
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- Child, Preschool, Female, Gene Expression Regulation, Gene Frequency genetics, Heterozygote, Homozygote, Humans, Hypersensitivity, Immediate genetics, Hypersensitivity, Immediate immunology, Immunity, Innate genetics, Immunity, Innate immunology, Infant, Newborn, Mothers, RNA, Messenger genetics, RNA, Messenger metabolism, Th2 Cells metabolism, Cytokines metabolism, Homeodomain Proteins genetics, Parturition genetics, Polymorphism, Single Nucleotide genetics, T-Box Domain Proteins genetics, Transcription Factors genetics
- Abstract
Background: TBX21 (T cell specific T-box transcription factor) and HLX1 (H.20-like homeobox 1) are crucial transcription factors of T(H)1-cells, inducing their differentiation and suppressing T(H)2 commitment, particularly important for early life immune development. This study investigated the influence of TBX21 and HLX1 single nucleotide polymorphisms (SNPs), which have previously been shown to be associated with asthma, on T(H)1/T(H)2 lineage cytokines at birth., Methods and Findings: Cord blood mononuclear cells (CBMCs) of 200 neonates were genotyped for two TBX21 and three HLX1 SNPs. CBMCs were stimulated with innate (Lipid A, LpA; Peptidoglycan, Ppg), adaptive stimuli (house dust mite Dermatophagoides pteronyssinus 1, Derp1) or mitogen (phytohemagglutinin, PHA). Cytokines, T-cells and mRNA expression of T(H)1/T(H)2-related genes were assessed. Atopic diseases during the first 3 years of life were assessed by questionnaire answered by the parents. Carriers of TBX21 promoter SNP rs17250932 and HLX1 promoter SNP rs2738751 showed reduced or trendwise reduced (p≤0.07) IL-5, IL-13 and TNF-α secretion after LpA-stimulation. Carriers of HLX1 SNP rs2738751 had lower IL-13 levels following Ppg-stimulation (p = 0.08). Carriers of HLX1 exon 1 SNP rs12141189 showed increased IL-5 (LpA, p = 0.007; Ppg, p = 0.10), trendwise increased IL-13 (LpA), higher GM-CSF (LpA/Ppg, p≤0.05) and trendwise decreased IFN-γ secretion (Derp1+LpA-stimulation, p = 0.1). Homozygous carriers of HLX1 promoter SNP rs3806325 showed increased IL-13 and IL-6 (unstimulated, p≤0.03). In carriers of TBX21 intron 3 SNP rs11079788 no differences in cytokine secretion were observed. mRNA expression of T(H)1/T(H)2-related genes partly correlated with cytokines at protein level. TBX21 SNP rs11079788 carriers developed less symptoms of atopic dermatitis at 3 years of age (p = 0.03)., Conclusions: Polymorphisms in TBX21 and HLX1 influenced primarily IL-5 and IL-13 secretion after LpA-stimulation in cord blood suggesting that genetic variations in the transcription factors essential for the T(H)1-pathway may contribute to modified T(H)2-immune responses already early in life. Further follow-up of the cohort is required to study the polymorphisms' relevance for immune-mediated diseases such as childhood asthma.
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- 2012
- Full Text
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12. Genetic variants harbored in the forkhead box protein 3 locus increase hay fever risk.
- Author
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Suttner K, Depner M, Wetzke M, Klopp N, von Mutius E, Illig T, Sparwasser T, and Kabesch M
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- Child, Gene Frequency, Genetic Variation, Genotype, Germany, Humans, Linkage Disequilibrium, Rhinitis, Allergic, Seasonal immunology, Rhinitis, Allergic, Seasonal physiopathology, Risk Factors, Forkhead Transcription Factors genetics, Rhinitis, Allergic, Seasonal genetics
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- 2010
- Full Text
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13. Genetic variants in the GATA3 gene are not associated with asthma and atopic diseases in German children.
- Author
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Suttner K, Depner M, Klopp N, Illig T, Vogelberg C, Adamski J, von Mutius E, and Kabesch M
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- Child, Gene Frequency genetics, Genetic Predisposition to Disease, Genotype, Germany epidemiology, Haplotypes genetics, Humans, Hypersensitivity epidemiology, Hypersensitivity immunology, Linkage Disequilibrium genetics, Polymorphism, Single Nucleotide genetics, Th2 Cells immunology, Th2 Cells metabolism, GATA3 Transcription Factor genetics, Hypersensitivity genetics
- Published
- 2009
- Full Text
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14. TBX21 gene variants increase childhood asthma risk in combination with HLX1 variants.
- Author
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Suttner K, Rosenstiel P, Depner M, Schedel M, Pinto LA, Ruether A, Adamski J, Klopp N, Illig T, Vogelberg C, Schreiber S, von Mutius E, and Kabesch M
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- Alleles, Asthma epidemiology, Asthma immunology, Case-Control Studies, Child, Cross-Sectional Studies, Genetic Predisposition to Disease, Genotype, Germany epidemiology, Humans, Linkage Disequilibrium genetics, Prevalence, Promoter Regions, Genetic, Asthma genetics, Gene Frequency genetics, Homeodomain Proteins genetics, Polymorphism, Single Nucleotide genetics, T-Box Domain Proteins genetics, Transcription Factors genetics
- Abstract
Background: The T cell-specific T-box transcription factor (TBX21) plays a crucial role in the regulation of the immune system because this factor induces the differentiation of T(H)1 and blocks T(H)2 commitment together with the homeobox transcription factor HLX1., Objective: The role of genetic variants in TBX21 alone and in combination with HLX1 polymorphisms was investigated in the development of T(H)2-associated atopy and asthma., Methods: The TBX21 gene was resequenced in 37 adult volunteers. Polymorphisms identified were genotyped in a cross-sectional (N = 3099) and nested asthma case-control population (N = 1872) using mainly matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Effects of promoter polymorphisms on TBX21 gene expression were studied by reporter gene assays. Furthermore, the impact of combinations of TBX21 and HLX1 polymorphisms on the development of asthma was assessed by using a risk score model. Statistical analyses were performed by using SAS/Genetics., Results: Forty-three polymorphisms were identified in the TBX21 gene. Considering a minor allele frequency of at least 10%, single nucleotide polymorphisms were assigned to 7 linkage disequilibrium blocks. Three tagging single nucleotide polymorphisms increased childhood asthma risk significantly (odds ratio [OR], 2.60, 95% CI, 1.34-5.03, P = .003; OR, 1.39, 95% CI, 1.02-1.90, P = .039; and OR, 1.97, 95% CI, 1.18-3.30, P = .009). TBX21 promoter polymorphisms contained in 2 blocks significantly influenced TBX21 promoter activity. In a risk score model, the combination of TBX21 and HLX1 polymorphisms increased the asthma risk by more than 3-fold., Conclusions: These data suggest that TBX21 polymorphisms contribute to the development of asthma, potentially by altering TBX21 promoter activity. A risk score model indicates that TBX21 and HLX1 polymorphisms may have synergistic effects on asthma risk.
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- 2009
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15. HLX1 gene variants influence the development of childhood asthma.
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Suttner K, Ruoss I, Rosenstiel P, Depner M, Pinto LA, Schedel M, Adamski J, Illig T, Schreiber S, von Mutius E, and Kabesch M
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- Asthma immunology, Asthma metabolism, Child, Cross-Sectional Studies, Female, GATA3 Transcription Factor genetics, GATA3 Transcription Factor immunology, GATA3 Transcription Factor metabolism, Homeodomain Proteins immunology, Homeodomain Proteins metabolism, Humans, Male, Promoter Regions, Genetic immunology, Risk Factors, Th1 Cells immunology, Th1 Cells metabolism, Th2 Cells immunology, Th2 Cells metabolism, Transcription Factors immunology, Transcription Factors metabolism, Asthma genetics, Homeodomain Proteins genetics, Polymorphism, Single Nucleotide, Promoter Regions, Genetic genetics, Transcription Factors genetics
- Abstract
Background: Major transcription factors controlling T(H)1 and T(H)2 development, such as T-box transcription factor and GATA3, might be centrally involved in asthma and atopic diseases. Only recently, the homeobox transcription factor H.20-like homeobox 1 (HLX1), interacting closely with T-box transcription factor, has been identified as an important regulator of T(H)1 differentiation and suppressor of T(H)2 commitment., Objective: We investigated whether genetic variations in the HLX1 gene exist and whether these could affect the development of childhood asthma., Methods: The HLX1 gene was resequenced in 80 chromosomes. Associations between identified polymorphisms, asthma, and atopic diseases were investigated in German children (total n = 3099) from the cross-sectional International Study of Asthma and Allergy in Childhood phase II. Functional properties of polymorphisms were studied by using luciferase reporter gene assays and electrophoretic mobility shift assays in T cells. All statistical analyses were performed with SAS/Genetics software (SAS Institute, Inc, Cary, NC)., Results: Nineteen polymorphisms were identified in the HLX1 gene, and 2 tagging single nucleotide polymorphisms representing 7 polymorphisms were associated with childhood asthma in our study population. Two promoter polymorphisms, C-1407T and C-742G, contained in 1 tagging block were associated with asthma (odds ratio, 1.44; 95% CI, 1.11-1.86; P = .0061), significantly decrease promoter transactivation, and disrupt specificity protein-transcription factor binding in in vitro experiments., Conclusions: Our data suggest that polymorphisms in the HLX1 gene increase the risk for childhood asthma. On the cellular level, altered binding of specificity protein-transcription factors to the HLX1 promoter and subsequent changes in HLX1 gene expression might contribute to these effects.
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- 2009
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16. TLR-dependent Bim phosphorylation in macrophages is mediated by ERK and is connected to proteasomal degradation of the protein.
- Author
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Häcker G, Suttner K, Harada H, and Kirschnek S
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- Animals, Bcl-2-Like Protein 11, Cell Line, HeLa Cells, Humans, Mice, Phosphorylation, Apoptosis Regulatory Proteins metabolism, Extracellular Signal-Regulated MAP Kinases metabolism, Macrophages metabolism, Membrane Proteins metabolism, Proteasome Endopeptidase Complex metabolism, Proto-Oncogene Proteins metabolism, Toll-Like Receptors metabolism
- Abstract
The pro-apoptotic Bcl-2 homology domain 3-only protein Bim has been shown to play an important role in immune cell homeostasis and various forms of apoptosis in the immune system. Bim is expressed in most immune cells, and regulation of Bim activity can occur on both transcriptional and post-translational levels. Toll-like receptor (TLR) stimulation has previously been shown to increase Bim expression and to cause Bim phosphorylation in the absence of apoptosis in mouse macrophages. Here we identify extracellular signal-regulated kinase as the major kinase responsible for TLR-dependent Bim phosphorylation. Three TLR-dependent serine phosphorylation sites, S55, S65 and S100, on mouse Bim were identified, two of them unique to the splice form Bim(EL) and one also present on Bim(L). A Bim mutant defective in these three phosphorylation sites showed slightly enhanced pro-apoptotic activity, which might indicate a protective effect of Bim phosphorylation in this system. Phosphorylation did not alter the association of Bim protein with the microtubule cytoskeleton. However, TLR-mediated phosphorylation led to accelerated degradation of Bim via the proteasome. Thus, TLR stimulation of macrophages can regulate Bim levels in opposing ways, namely by transcriptional induction and by phosphorylation-dependent degradation of the protein.
- Published
- 2006
- Full Text
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