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We investigated the fourth subgroup of Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-β-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions.

This paper aims to propose ideas to improve the land-use and management of Japanese suburban areas based on an investigation of development trends in Kiyotake town, a local town in the suburban area of a prefectural capital. Building permits during 1995 –1999 were analyzed by use of a geographic information system (GIS). The major results were as follows: 1) There were numerous undesirable developments in the urbanization control area and outside of the city planning area. 2) This was because of loose land-use management by the town authority, and loopholes in the conventional City Planning Law. 3) The City Planning Law 2000 has several features such as deregulation, decentralization and public involvement. However, the features have potentially deleterious effects. To achieve desirable land-use, stricter management should be adopted based on the strict stipulations and regulations of the Law. This management requires deliberate public involvement that attaches importance to the public interest., Proceedings of the Eastern Asia Society for Transportation Studies (EASTS '03), October 29-November 1, 2003, Fukuoka International Congress Center, Japan