1. A Technique for Performing Electrical Impedance Myography in the Mouse Hind Limb: Data in Normal and ALS SOD1 G93A Animals
- Author
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Minhee Sung, Wayne L. Staats, Seward B. Rutkove, Jia Li, Andrew J. Spieker, Massachusetts Institute of Technology. Department of Mechanical Engineering, and Staats, Wayne Lawrence
- Subjects
Male ,Neurology ,Anatomy and Physiology ,lcsh:Medicine ,Electromyography ,Hindlimb ,Muscular Dystrophies ,Motor Neuron Diseases ,Mice ,0302 clinical medicine ,Engineering ,Electric Impedance ,Medicine ,Amyotrophic lateral sclerosis ,lcsh:Science ,0303 health sciences ,Multidisciplinary ,medicine.diagnostic_test ,Electrical impedance myography ,Neurodegenerative Diseases ,Anatomy ,Repeatability ,Neuromuscular Diseases ,Electrophysiology ,Female ,Research Article ,Test Evaluation ,medicine.medical_specialty ,Neuromuscular disease ,03 medical and health sciences ,Sod1 g93a ,Diagnostic Medicine ,Animals ,Biology ,030304 developmental biology ,Motor Systems ,business.industry ,Superoxide Dismutase ,Mechanical Engineering ,lcsh:R ,Amyotrophic Lateral Sclerosis ,Myography ,Reproducibility of Results ,medicine.disease ,Mutation ,Anisotropy ,lcsh:Q ,business ,030217 neurology & neurosurgery ,Biomedical engineering ,Neuroscience - Abstract
Objective: To test a method for performing electrical impedance myography (EIM) in the mouse hind limb for the assessment of disease status in neuromuscular disease models. Methods: An impedance measuring device consisting of a frame with electrodes embedded within an acrylic head was developed. The head was rotatable such that data longitudinal and transverse to the major muscle fiber direction could be obtained. EIM measurements were made with this device on 16 healthy mice and 14 amyotrophic lateral sclerosis (ALS) animals. Repeatability was assessed in both groups. Results: The technique was easy to perform and provided good repeatability in both healthy and ALS animals, with intra-session repeatability (mean ± SEM) of 5% ±1% and 12% ±2%, respectively. Significant differences between healthy and ALS animals were also identified (e.g., longitudinal mean 50 kHz phase was 18±0.6° for the healthy animals and 14±1.0° for the ALS animals, p = 0.0025). Conclusions: With this simple device, the EIM data obtained is highly repeatable and can differentiate healthy from ALS animals. Significance: EIM can now be applied to mouse models of neuromuscular disease to assess disease status and the effects of therapy.
- Published
- 2012