12 results on '"Singh, Rahul Mohan"'
Search Results
2. The m6A reader ECT1 drives mRNA sequestration to dampen salicylic acid–dependent stress responses in Arabidopsis.
- Author
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Lee, Keun Pyo, Liu, Kaiwei, Kim, Eun Yu, Medina-Puche, Laura, Dong, Haihong, Di, Minghui, Singh, Rahul Mohan, Li, Mengping, Qi, Shan, Meng, Zhuoling, Cho, Jungnam, Zhang, Heng, Lozano-Duran, Rosa, and Kim, Chanhong
- Published
- 2024
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- View/download PDF
3. Cooperative role of AtRsmD and AtRimM proteins in modification and maturation of 16S rRNA in plastids.
- Author
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Liu, Kaiwei, Lee, Keun Pyo, Duan, Jianli, Kim, Eun Yu, Singh, Rahul Mohan, Di, Minghui, Meng, Zhuoling, and Kim, Chanhong
- Subjects
RIBOSOMAL RNA ,PLASTIDS ,GENETIC translation ,NONSENSE mutation ,RNA modification & restriction ,PROTEINS ,RIBOSOMES - Abstract
SUMMARY: Chloroplast pre‐ribosomal RNA (rRNA) undergoes maturation, which is critical for ribosome assembly. While the central and auxiliary factors in rRNA maturation have been elucidated in bacteria, their mode of action remains largely unexplored in chloroplasts. We now reveal chloroplast‐specific factors involved in 16S rRNA maturation, Arabidopsis thaliana orthologs of bacterial RsmD methyltransferase (AtRsmD) and ribosome maturation factor RimM (AtRimM). A forward genetic screen aimed to find suppressors of the Arabidopsis yellow variegated 2 (var2) mutant defective in photosystem II quality control found a causal nonsense mutation in AtRsmD. The substantially impaired 16S rRNA maturation and translation due to the mutation rescued the leaf variegation phenotype by lowering the levels of chloroplast‐encoded proteins, including photosystem II core proteins, in var2. The subsequent co‐immunoprecipitation coupled with mass spectrometry analyses and bimolecular fluorescence complementation assay found that AtRsmD interacts with AtRimM. Consistent with their interaction, loss of AtRimM also considerably impairs 16S rRNA maturation with decelerated m2G915 modification in 16S rRNA catalyzed by AtRsmD. The atrimM mutation also rescued var2 mutant phenotypes, corroborating the functional interplay between AtRsmD and AtRimM towards modification and maturation of 16S rRNA and chloroplast proteostasis. The maturation and post‐transcriptional modifications of rRNA are critical to assembling ribosomes responsible for protein translation. Here, we revealed that the cooperative regulation of 16S rRNA m2G915 modifications by AtRsmD methyltransferase and ribosome assembly factor AtRimM contributes to 16S rRNA maturation, ribosome assembly, and proteostasis in chloroplasts. Significance Statement: The maturation and post‐transcriptional modifications of ribosomal RNA (rRNA) are critical to assembling ribosomes responsible for protein translation. Here, we revealed that the cooperative regulation of 16S rRNA m2G915 modifications by AtRsmD methyltransferase and ribosome assembly factor AtRimM contributes to 16S rRNA maturation, ribosome assembly, and proteostasis in chloroplasts. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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4. Determination of Major Viral and Sub Viral Pathogens Incidence in Apple Orchards in Himachal Pradesh
- Author
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Kumar, Surender, Singh, Rahul Mohan, Ram, Raja, Badyal, J., Hallan, Vipin, Zaidi, A. A., and Varma, Anupam
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- 2012
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5. Complete nucleotide sequence of cherry virus A (CVA) infecting sweet cherry in India
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Noorani, M. S., Awasthi, P., Singh, Rahul Mohan, Ram, Raja, Sharma, M. P., Singh, S. R., Ahmed, N., Hallan, V., and Zaidi, A. A.
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- 2010
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6. EXECUTER2 modulates the EXECUTER1 signalosome through its singlet oxygen-dependent oxidation.
- Author
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Dogra, Vivek, Singh, Rahul Mohan, Li, Mengping, Li, Mingyue, Singh, Somesh, and Kim, Chanhong
- Abstract
Oxidative post-translational modifications of specific chloroplast proteins contribute to the initiation of retrograde signaling. The Arabidopsis thaliana EXECUTER1 (EX1) protein, a chloroplast-localized singlet oxygen (1O 2) sensor, undergoes tryptophan (Trp) 643 oxidation by 1O 2 , a chloroplast-derived and light-dependent reactive oxygen species. The indole side chain of Trp is vulnerable to 1O 2 , leading to the generation of oxidized Trp variants and priming EX1 for degradation by a membrane-bound FtsH protease. The perception of 1O 2 via Trp643 oxidation and subsequent EX1 proteolysis facilitate chloroplast-to-nucleus retrograde signaling. In this study, we discovered that the EX1-like protein EX2 also undergoes 1O 2 -dependent Trp530 oxidation and FtsH-dependent turnover, which attenuates 1O 2 signaling by decelerating EX1-Trp643 oxidation and subsequent EX1 degradation. Consistent with this finding, the loss of EX2 function reinforces EX1-dependent retrograde signaling by accelerating EX1-Trp643 oxidation and subsequent EX1 proteolysis, whereas overexpression of EX2 produces molecular phenotypes opposite to those observed in the loss–of- function mutants of EX2. Intriguingly, phylogenetic analysis suggests that EX2 may have emerged evolutionarily to attenuate the sensitivity of EX1 toward 1O 2. Collectively, these results suggest that EX2 functions as a negative regulator of the EX1 signalosome through its own 1O 2 -dependent oxidation, providing a new mechanistic insight into the regulation of EX1-mediated 1O 2 signaling. EXECUTER1 (EX1) and EX2 proteins have long been considered major players in mediating singlet oxygen (1O 2)-triggered chloroplast-to-nucleus retrograde signaling, but the specific mechanisms by which they regulate 1O 2 signaling have been unclear. This study demonstrates that the 1O 2 -driven oxidative modification of EX2 modulates EX1-mediated 1O 2 signaling by hindering the oxidation of EX1 that is essential for the initiation of 1O 2 signaling. [ABSTRACT FROM AUTHOR]
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- 2022
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7. FATTY ACID DESATURASE5 Is Required to Induce Autoimmune Responses in Gigantic Chloroplast Mutants of Arabidopsis.
- Author
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Li, Bingqi, Fang, Jun, Singh, Rahul Mohan, Zi, Hailing, Lv, Shanshan, Liu, Renyi, Dogra, Vivek, and Kim, Chanhong
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- 2020
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8. Molecular diagnosis of apple virus and viroid pathogens from India.
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Rana, Tanuja, Negi, Anuradha, Dhir, Sunny, Thockchom, Tombisana, Chandel, Vanita, Walia, Yashika, Singh, Rahul Mohan, Ram, Raja, Hallan, Vipin, and Zaidi, A. A.
- Subjects
APPLE varieties ,MOSAIC viruses ,MOLECULAR diagnosis ,VIROID diseases of plants ,DISEASE susceptibility ,ENZYME-linked immunosorbent assay ,PLANT nurseries - Abstract
Apple is known to be susceptible to various virus and viroid pathogens. Symptomatic apple cultivars and rootstocks were collected and analyzed by ELISA and then through RT-PCR. The study reports the presence of Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV), the major apple viruses and Prunus necrotic ringspot virus (PNRSV), a minor apple virus, at the molecular level in India. Apple scar skin viroid (ASSVd) infection was also confirmed at the molecular level. Sporadic incidences of Tomato ringspot virus and Arabis mosaic virus infections were also detected by ELISA in nursery plants. [ABSTRACT FROM AUTHOR]
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- 2011
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9. Homo- and Hetero-Dimers of CAD Enzymes Regulate Lignification and Abiotic Stress Response in Moso Bamboo.
- Author
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Vasupalli, Naresh, Hou, Dan, Singh, Rahul Mohan, Wei, Hantian, Zou, Long-Hai, Yrjälä, Kim, Wu, Aimin, and Lin, Xinchun
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ABIOTIC stress ,MULTIENZYME complexes ,LIGNIFICATION ,ENZYMES ,ALCOHOL dehydrogenase ,SALICYLIC acid - Abstract
Lignin biosynthesis enzymes form complexes for metabolic channelling during lignification and these enzymes also play an essential role in biotic and abiotic stress response. Cinnamyl alcohol dehydrogenase (CAD) is a vital enzyme that catalyses the reduction of aldehydes to alcohols, which is the final step in the lignin biosynthesis pathway. In the present study, we identified 49 CAD enzymes in five Bambusoideae species and analysed their phylogenetic relationships and conserved domains. Expression analysis of Moso bamboo PheCAD genes in several developmental tissues and stages revealed that among the PheCAD genes, PheCAD2 has the highest expression level and is expressed in many tissues and PheCAD1, PheCAD6, PheCAD8 and PheCAD12 were also expressed in most of the tissues studied. Co-expression analysis identified that the PheCAD2 positively correlates with most lignin biosynthesis enzymes, indicating that PheCAD2 might be the key enzyme involved in lignin biosynthesis. Further, more than 35% of the co-expressed genes with PheCADs were involved in biotic or abiotic stress responses. Abiotic stress transcriptomic data (SA, ABA, drought, and salt) analysis identified that PheCAD2, PheCAD3 and PheCAD5 genes were highly upregulated, confirming their involvement in abiotic stress response. Through yeast two-hybrid analysis, we found that PheCAD1, PheCAD2 and PheCAD8 form homo-dimers. Interestingly, BiFC and pull-down experiments identified that these enzymes form both homo- and hetero- dimers. These data suggest that PheCAD genes are involved in abiotic stress response and PheCAD2 might be a key lignin biosynthesis pathway enzyme. Moreover, this is the first report to show that three PheCAD enzymes form complexes and that the formation of PheCAD homo- and hetero- dimers might be tissue specific. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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10. FERONIA regulates salt tolerance in Arabidopsis by controlling photorespiratory flux.
- Author
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Jiang W, Wang Z, Li Y, Liu X, Ren Y, Li C, Luo S, Singh RM, Li Y, Kim C, and Zhao C
- Abstract
Photorespiration is an energetically costly metabolic pathway in plants that responds to environmental stresses. The molecular basis of the regulation of the photorespiratory cycle under stress conditions remains unclear. Here, we discovered that FERONIA (FER) regulates photorespiratory flow under salt stress in Arabidopsis (Arabidopsis thaliana). FER mutation results in hypersensitivity to salt stress, but disruption of ferredoxin-dependent glutamate synthase 1 (GLU1), an enzyme that participates in the photorespiratory pathway by producing glutamate, greatly suppresses fer-4 hypersensitivity to salt stress primarily due to reduced glycine yield. In contrast, disrupting mitochondrial serine hydroxymethyltransferase1 (SHM1), which is supposed to increase glycine levels by hampering the conversion of glycine to serine in the photorespiratory cycle, aggravates fer-4 hypersensitivity to salt stress. Biochemical data show that FER interacts with and phosphorylates SHM1, and this phosphorylation modulates SHM1 stability. Additionally, the production of proline and its intermediate △1-pyrroline-5-carboxylate (P5C), which are both synthesized from glutamate, also contributes to fer-4 hypersensitivity to salt stress. In conclusion, this study elucidates the functional mechanism of FER in regulating salt tolerance by modulating photorespiratory flux, which greatly broadens our understanding of how plants adapt to high salinity., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
- Published
- 2024
- Full Text
- View/download PDF
11. The m6A reader ECT1 drives mRNA sequestration to dampen salicylic acid-dependent stress responses in Arabidopsis.
- Author
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Lee KP, Liu K, Kim EY, Medina-Puche L, Dong H, Di M, Singh RM, Li M, Qi S, Meng Z, Cho J, Zhang H, Lozano-Duran R, and Kim C
- Subjects
- Salicylic Acid metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Gene Expression Regulation, Plant, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Adenine analogs & derivatives
- Abstract
N 6-methyladenosine (m6A) is a common epitranscriptional mRNA modification in eukaryotes. Thirteen putative m6A readers, mostly annotated as EVOLUTIONARILY CONSERVED C-TERMINAL REGION (ECT) proteins, have been identified in Arabidopsis (Arabidopsis thaliana), but few have been characterized. Here, we show that the Arabidopsis m6A reader ECT1 modulates salicylic acid (SA)-mediated plant stress responses. ECT1 undergoes liquid-liquid phase separation in vitro, and its N-terminal prion-like domain is critical for forming in vivo cytosolic biomolecular condensates in response to SA or bacterial pathogens. Fluorescence-activated particle sorting coupled with quantitative PCR analyses unveiled that ECT1 sequesters SA-induced m6A modification-prone mRNAs through its conserved aromatic cage to facilitate their decay in cytosolic condensates, thereby dampening SA-mediated stress responses. Consistent with this finding, ECT1 overexpression promotes bacterial multiplication in plants. Collectively, our findings unequivocally link ECT1-associated cytosolic condensates to SA-dependent plant stress responses, advancing the current understanding of m6A readers and the SA signaling network., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2024
- Full Text
- View/download PDF
12. Movement protein of Apple chlorotic leaf spot virus is genetically unstable and negatively regulated by Ribonuclease E in E. coli.
- Author
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Singh RM, Singh D, and Hallan V
- Subjects
- Escherichia coli metabolism, Escherichia coli virology, Flexiviridae genetics, Plant Viral Movement Proteins genetics, Proteolysis, RNA Stability, Endoribonucleases metabolism, Escherichia coli Proteins metabolism, Flexiviridae metabolism, Plant Viral Movement Proteins metabolism
- Abstract
Movement protein (MP) of Apple chlorotic leaf spot virus (ACLSV) belongs to "30 K" superfamily of proteins and members of this family are known to show a wide array of functions. In the present study this gene was found to be genetically unstable in E. coli when transformed DH5α cells were grown at 28 °C and 37 °C. However, genetic instability was not encountered at 20 °C. Heterologous over expression failed despite the use of different transcriptional promoters and translational fusion constructs. Total cell lysate when subjected to western blotting using anti-ACLSV MP antibodies, showed degradation/cleavage of the expressed full-length protein. This degradation pointed at severe proteolysis or instability of the corresponding mRNA. Predicted secondary structure analysis of the transcript revealed a potential cleavage site for an endoribonuclease (RNase E) of E. coli. The negating effect of RNase E on transcript stability and expression was confirmed by northern blotting and quantitative RT-PCR of the RNA extracted from RNase E temperature sensitive mutant (strain N3431). The five fold accumulation of transcripts at non-permissive temperature (43 °C) suggests the direct role of RNase E in regulating the expression of ACLSV MP in E. coli.
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- 2017
- Full Text
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