48 results on '"Sinclair, Adriane"'
Search Results
2. Reduced Protein Import via TIM23 SORT Drives Disease Pathology in TIMM50-Associated Mitochondrial Disease.
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Crameri, Jordan J., Palmer, Catherine S., Stait, Tegan, Jackson, Thomas D., Lynch, Matthew, Sinclair, Adriane, Frajman, Leah E., Compton, Alison G., Coman, David, Thorburn, David R., Frazier, Ann E., and Stojanovski, Diana
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MITOCHONDRIAL pathology ,PATHOLOGY ,BIOCHEMICAL substrates ,MITOCHONDRIAL membranes ,OXIDATIVE phosphorylation - Abstract
TIMM50 is a core subunit of the TIM23 complex, the mitochondrial inner membrane translocase responsible for the import of pre-sequence-containing precursors into the mitochondrial matrix and inner membrane. Here we describe a mitochondrial disease patient who is homozygous for a novel variant in TIMM50 and establish the first proteomic map of mitochondrial disease associated with TIMM50 dysfunction. We demonstrate that TIMM50 pathogenic variants reduce the levels and activity of endogenous TIM23 complex, which significantly impacts the mitochondrial proteome, resulting in a combined oxidative phosphorylation (OXPHOS) defect and changes to mitochondrial ultrastructure. Using proteomic data sets from TIMM50 patient fibroblasts and a TIMM50 HEK293 cell model of disease, we reveal that laterally released substrates imported via the TIM23
SORT complex pathway are most sensitive to loss of TIMM50. Proteins involved in OXPHOS and mitochondrial ultrastructure are enriched in the TIM23SORT substrate pool, providing a biochemical mechanism for the specific defects in TIMM50-associated mitochondrial disease patients. These results highlight the power of using proteomics to elucidate molecular mechanisms of disease and uncovering novel features of fundamental biology, with the implication that human TIMM50 may have a more pronounced role in lateral insertion than previously understood. [ABSTRACT FROM AUTHOR]- Published
- 2024
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3. Late-Onset Group B Streptococcal Meningitis Has Cerebrovascular Complications
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Tibussek, Daniel, Sinclair, Adriane, Yau, Ivanna, Teatero, Sarah, Fittipaldi, Nahuel, Richardson, Susan E., Mayatepek, Ertan, Jahn, Peter, and Askalan, Rand
- Published
- 2015
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4. Canalization of the Urethral Plate Precedes Fusion of the Urethral Folds during Male Penile Urethral Development: The Double Zipper Hypothesis
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Li, Yi, Sinclair, Adriane, Cao, Mei, Shen, Joel, Choudhry, Shweta, Botta, Sisir, Cunha, Gerald, and Baskin, Laurence
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- 2015
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5. Canalization of the Vestibular Plate in the Absence of Urethral Fusion Characterizes Development of the Human Clitoris: The Single Zipper Hypothesis
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Overland, Maya, Li, Yi, Cao, Mei, Shen, Joel, Yue, Xuan, Botta, Sisir, Sinclair, Adriane, Cunha, Gerald, and Baskin, Laurence
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- 2016
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6. Expression Analysis of DGKK during External Genitalia Formation
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Shen, Joel, Liu, Baomei, Sinclair, Adriane, Cunha, Gerald, Baskin, Laurence S., and Choudhry, Shweta
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- 2015
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7. Current understanding of hypospadias: relevance of animal models
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Cunha, Gerald R., Sinclair, Adriane, Risbridger, Gail, Hutson, John, and Baskin, Laurence S.
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- 2015
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8. Variation in Penile and Clitoral Morphology in Four Species of Moles
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Sinclair, Adriane Watkins
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Morphology ,Physiology ,Endocrinology ,clitoris ,mole ,ovarian interstitial gland ,penile clitoris ,penis ,Talpa - Abstract
Most eutherian mammals possess sexually dimorphic external genitalia. Males have a penis that is traversed to near the tip by a urethra, a scrotum that encloses the testes, and a long anogenital distance. In females anogenital distance is short, and the typical clitoris is usually markedly smaller than the penis, and is frequently "internally" situated with the urethra exiting independent of the clitoris. In addition, the clitoris is associated with an externally visible vaginal opening (at least during the breeding season). This sexual dimorphism is usually associated with the presence (males) or absence (females) of androgens during development of the external genitalia. Females with naturally "masculinized" external genitalia challenge the typical mammalian androgen-dependent masculinization theory and are the focus of this dissertation. Research as to how they "do," or "do-not" fit the current widely accepted theory of sexual differentiation may reveal novel mechanisms of sexual differentiation. Since adult external genitalia are the endpoints of sexual differentiation, developmental processes can be inferred from examination of adult morphology, providing that anatomy of external genitalia has been (is) accurately described and interpreted. The first revelation incurred in my study was that all previous reports on mole external genitalia were in error in regard to the following terms: penis, clitoris, penile clitoris, phallus, prepuce and urethra. Accordingly, by way of correcting errors of previous literature, my first task was a detailed anatomic and morphometric analysis of external genitalia in four species of moles. For an even broader perspective I also reviewed morphology of external genitalia of mouse, human and spotted hyena. Accurate morphological descriptions of external genitalia are the essential pre-requisite to a full understanding of the comparative anatomy of mole external genitalia and the potential role of hormones in development of the external genitalia in these species.This dissertation is focused on four species of moles. All are members of the family Talpidae, in the order Insectivora. Three of these species defy the conventionally obvious visual distinctions between males and females in so far as the perineal appendage described previously as "penis" and "penile clitoris" is of similar size in males and females. Unfortunately, the visible perineal appendage in these male and female moles is prepuce. A major question is why the prepuce is similar in size in males and females. One theory is that the ovarian interstitial gland is capable of producing androgen. Of the three mole species with "masculinized" female external genitalia, broad-footed moles (Scapanus latimanus) do not possess an ovarian interstitial gland, star-nosed moles (Condylura cristata) have an ovarian interstitial gland, and in hairy-tailed moles (Parascalops breweri) ovarian structure has never been investigated. For these three mole species, it is difficult for the casual observer to distinguish between males and females during the non-breeding season, when the vaginal opening of female moles is "closed." The fourth species examined in the present investigation is the Japanese shrew mole (Urotrichus talpoides), which does not possess an ovarian interstitial gland. In Japanese shrew moles, the distinction between males and females is obvious, since the male prepuce is much larger than the female prepuce, and the latter does not have a urethra exiting its tip. Thus, sexual dimorphism of the external genitalia in Japanese shrew moles appears to follow the typical mammalian pattern.Intriguing work has been done on mole species concerning the ovarian interstitial gland, its resemblance to testicular tissue, and its ability to produce androgens in relation to the corresponding presence of a "penile clitoris" in some mole species. However, other mole species that do not possess this ovarian interstitial gland also display a "penile clitoris" that is similar in size and shape to male external genitalia. Of course, this erroneous discussion of "penile clitoris" from the literature actually deals with the female prepuce. Discussion of the role of the ovarian interstitial gland in masculinization of mole external genitalia, first and foremost requires a detailed accurate anatomic analysis of the structure of the so-called "penile clitoris". Unfortunately no comparative work has adequately addressed the role of androgens derived from the ovarian interstitial gland in development of external genitalia in the different mole species. For the first time, my accurate detailed description of mole genital morphology provides the opportunity to address this question. The penis and clitoris of typical mammals are strikingly different, anatomically complex organs composed of epithelial tissue, connective tissue, vascular tissue, nerves, cartilage, and bone that are organized into specific and precise morphological patterns. The common developmental history, architecture, and composition of the penis and clitoris across most mammalian species allow for multiple features to be used to assess sexual dimorphism of the various components that constitute male and female external genitalia. The size and location of several key anatomic features were noted with the aid of three-dimensional reconstructions for a more detailed comparison between the different mole species. In addition to these internal anatomical measurements, anogenital distance, a trait that is modulated by androgen action in utero, was used as another measure of "genital masculinization". Measures of prepuce length, termed "phallus" length in previous publications, were used to investigate the degree of sexual dimorphism in the external genitalia in my study. Ovarian tissue was examined histologically for the presence or absence of an interstitial gland (a potential source of androgen). I discovered in the breeding season, Japanese shrew moles display a large glandular tissue structure attached to the ovary that has never before been reported. The Japanese shrew mole penis is much larger than the clitoris, is vastly anatomically different from the clitoris, and males have a longer anogenital distance than females presenting a typical mammalian pattern of sexual dimorphism, presumably based upon the presence versus absence of adequate androgen levels. Broad-footed, star-nosed, and hairy-tailed moles have notable morphological variation in the penis and clitoris between these species as well as between the sexes. However, similar to star-nosed moles (that possess an ovarian interstitial gland), female broad-footed moles displayed several masculine morphological characteristics and an anogenital distance equal to the males' despite lacking an ovarian interstitial gland. This suggests that either development of the external genitalia is partially androgen-independent in these species or in females androgen production may be coming from another source. Lastly, I compared different patterns of external genitalia in human, mouse, 4 species of mole, and spotted hyena in relation to known endocrine profiles, and mechanisms of morphogenesis/differentiation noting gaps in the data.In summary, my research provides the first accurate descriptions of the gross and histologic anatomy of male and female mole external genitalia. Also, comparative mouse-mole studies reported here have validated the remarkable similarity in external genitalia anatomy between these two species and have led to the conclusion that all previous literature on mole external genitalia suffers from consistent anatomical error. Being able to set the record straight allows for the first time an accurate definition of mole external genitalia anatomy and its relation to endocrine parameters.
- Published
- 2014
9. ‘Beyond Milestones’: A randomised controlled trial evaluating an innovative digital resource teaching quality observation of normal child development
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Connolly, Anne M, Cunningham, Clare, Sinclair, Adriane J, Rao, Arjun, Lonergan, Amy, and Bye, Ann ME
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- 2014
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10. Clinical association of intrathecal and mirrored oligoclonal bands in paediatric neurology
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SINCLAIR, ADRIANE J, WIENHOLT, LOUISE, TANTSIS, ESTHER, BRILOT, FABIENNE, and DALE, RUSSELL C
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- 2013
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11. Cerebrospinal fluid neopterin as a biomarker of treatment response to Janus kinase inhibition in Aicardi–Goutières syndrome.
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Han, Velda X, Mohammad, Shekeeb S, Jones, Hannah F, Bandodkar, Sushil, Crow, Yanick J, Dale, Russell C, Sharpe, Cynthia, Sinclair, Adriane, Sinclair, Kate, Srinivasan, Deepa, Troedson, Christopher, Wong, Melanie, and Chaitow, Jeffrey
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CEREBROSPINAL fluid ,NEOPTERIN ,BIOMARKERS ,CELL communication ,NEUROLOGICAL disorders - Abstract
Janus kinase (JAK) 1 inhibition represents a precision medicine approach in the treatment of Aicardi–Goutières syndrome (AGS), through targeting of type I interferon‐mediated cell signalling. Blood interferon mRNAseq has been proposed as a biomarker of disease with utility in therapeutic monitoring. Objective cerebrospinal fluid (CSF) biomarkers tracking treatment efficacy are currently lacking. Here, we report a retrospective case series of 13 patients (median age 6y, range 2y 6mo–17y; five females, eight males) with AGS demonstrating significantly elevated CSF neopterin levels at first sampling (median 200nmol/L, range 45–2024nmol/L), compared to 13 age‐matched controls with non‐inflammatory neurological conditions (median 23nmol/L, range 5–34nmol/L, p<0.001). Five patients with AGS treated with JAK inhibitors demonstrated a median 81.5% reduction of CSF neopterin (range –36% to –88% change from baseline), compared to eight untreated patients with AGS demonstrating a median 7% reduction in CSF neopterin (range –63% to +117% change) (p=0.047). Our data indicate a biological effect of JAK inhibitors, and the potential role of CSF neopterin as a biomarker of treatment response. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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12. Immune-mediated steroid-responsive epileptic spasms and epileptic encephalopathy associated with VGKC-complex antibodies
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SULEIMAN, JEHAN, BRENNER, TANJA, GILL, DEEPAK, TROEDSON, CHRISTOPHER, SINCLAIR, ADRIANE J, BRILOT, FABIENNE, VINCENT, ANGELA, LANG, BETHAN, and DALE, RUSSELL C
- Published
- 2011
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13. A critical role for estrogen signaling in penis development.
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Govers, Luke C., Phillips, Tiffany R., Mattiske, Deidre M., Rashoo, Nineveh, Black, Jay R., Sinclair, Adriane, Baskin, Laurence S., Risbridger, Gail P., and Pask, Andrew J.
- Published
- 2019
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14. Australian Clinical Consensus Guideline: The diagnosis and acute management of childhood stroke.
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Medley, Tanya L., Mackay, Mark T., Cheung, Michael, Monagle, Paul, Mandelstam, Simone, Stojanovski, Belinda, Dale, Russell C., Fahey, Michael, Sinclair, Adriane, Walsh, Peter, Wray, Alison, Miteff, Christina, Andrews, Ian, Ware, Tyson, Pridmore, Clair, and Troedson, Christopher
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CHILD mortality ,STROKE ,CONGENITAL heart disease ,CHILDREN ,MAGNETIC resonance imaging - Abstract
Stroke is among the top 10 causes of death in children and survivors carry resulting disabilities for decades, at substantial cost to themselves and their families. Children are not currently able to access reperfusion therapies, due to limited evidence supporting safety and efficacy and long diagnostic delays. The Australian Clinical Consensus Guideline for the Diagnosis and Acute Management of Childhood Stroke was developed to minimize unwarranted variations in care and document best evidence on the risk factors, etiologies, and conditions mimicking stroke that differ from adults. Clinical questions were formulated to inform systematic database searches from 2007 to 2017, limited to English and pediatric studies. SIGN methodology and the National Health and Medical Research Council system were used to screen and classify the evidence. The Grades of Recommendation, Assessment, Development, and Evaluation system (GRADE) was used to grade evidence as strong or weak. The Guideline provides more than 60 evidence-based recommendations to assist prehospital and acute care clinicians in the rapid identification of childhood stroke, choice of initial investigation, to confirm diagnosis, determine etiology, selection of the most appropriate interventions to salvage brain at risk, and prevent recurrence. Recommendations include advice regarding the management of intracranial pressure and congenital heart disease. Implementation of the Guideline will require reorganization of prehospital and emergency care systems, including the development of regional stroke networks, pediatric Code Stroke, rapid magnetic resonance imaging and accreditation of primary pediatric stroke centers with the capacity to offer reperfusion therapies. The Guideline will allow auditing to benchmark timelines of care, access to acute interventions, and outcomes. It will also facilitate the development of an Australian childhood stroke registry, with data linkage to international registries, to allow for accurate data collection on stroke incidence, treatment, and outcomes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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15. Trigeminal autonomic cephalgia: A rare cause of headache in children.
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Stringer, Josephine A., Calvert, Sophie, and Sinclair, Adriane
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HEADACHE in children ,PEDIATRIC neurology ,CLUSTER headache ,BOYS ,HEADACHE ,SOCIETIES ,DISEASES ,ANALGESICS ,DIFFERENTIAL diagnosis ,LONGITUDINAL method ,MAGNETIC resonance imaging ,MIGRAINE ,RISK assessment ,TIME ,DISEASE relapse ,DISEASE remission ,SEVERITY of illness index ,PRIMARY headache disorders ,DISEASE complications - Abstract
The article describes the case of a two-year-old boy who was diagnosed of trigeminal autonomic cephalgia (TAC). Topics discussed include characteristics of TAC, definition given by the International Headache Society on TACs, and classifications of TACs including cluster headache (CH), paroxysmal hemicranias, and hemicrania continua.
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- 2018
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16. Comparative Morphology of the Penis and Clitoris in Four Species of Moles (Talpidae).
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Sinclair, Adriane Watkins, Glickman, Stephen, Catania, Kenneth, Shinohara, Akio, Baskin, Lawrence, and Cunha, Gerald R.
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MOLES (Animals) ,CLITORIS ,PENIS physiology ,GENITALIA ,ANIMAL morphology ,PHYSIOLOGY - Abstract
ABSTRACT The penile and clitoral anatomy of four species of Talpid moles (broad-footed, star-nosed, hairy-tailed, and Japanese shrew moles) were investigated to define penile and clitoral anatomy and to examine the relationship of the clitoral anatomy with the presence or absence of ovotestes. The ovotestis contains ovarian tissue and glandular tissue resembling fetal testicular tissue and can produce androgens. The ovotestis is present in star-nosed and hairy-tailed moles, but not in broad-footed and Japanese shrew moles. Using histology, three-dimensional reconstruction, and morphometric analysis, sexual dimorphism was examined with regard to a nine feature masculine trait score that included perineal appendage length (prepuce), anogenital distance, and presence/absence of bone. The presence/absence of ovotestes was discordant in all four mole species for sex differentiation features. For many sex differentiation features, discordance with ovotestes was observed in at least one mole species. The degree of concordance with ovotestes was highest for hairy-tailed moles and lowest for broad-footed moles. In relationship to phylogenetic clade, sex differentiation features also did not correlate with the similarity/divergence of the features and presence/absence of ovotestes. Hairy-tailed and Japanese shrew moles reside in separated clades, but they exhibit a high degree of congruence. Broad-footed and hairy-tailed moles reside within the same clade but had one of the lowest correlations in features and presence/absence of ovotestes. Thus, phylogenetic affinity and the presence/absence of ovotestes are poor predictors for most sex differentiation features within mole external genitalia. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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17. Anatomy of mole external genitalia: Setting the record straight.
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Sinclair, Adriane Watkins, Glickman, Stephen E., Baskin, Laurence, and Cunha, Gerald R.
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- 2016
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18. Stroke in Children With Cardiac Disease: Report From the International Pediatric Stroke Study Group Symposium.
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Sinclair, Adriane J., Fox, Christine K., Ichord, Rebecca N., Almond, Christopher S., Bernard, Timothy J., Beslow, Lauren A., Chan, Anthony K.C., Cheung, Michael, deVeber, Gabrielle, Dowling, Michael M., Friedman, Neil, Giglia, Therese M., Guilliams, Kristin P., Humpl, Tilman, Licht, Daniel J., Mackay, Mark T., and Jordan, Lori C.
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HEART diseases , *PEDIATRICS , *STROKE prevention , *DISEASE incidence , *COMPUTED tomography , *ULTRASONIC imaging - Abstract
Background Cardiac disease is a leading cause of stroke in children, yet limited data support the current stroke prevention and treatment recommendations. A multidisciplinary panel of clinicians was convened in February 2014 by the International Pediatric Stroke Study group to identify knowledge gaps and prioritize clinical research efforts for children with cardiac disease and stroke. Results Significant knowledge gaps exist, including a lack of data on stroke incidence, predictors, primary and secondary stroke prevention, hyperacute treatment, and outcome in children with cardiac disease. Commonly used diagnostic techniques including brain computed tomography and ultrasound have low rates of stroke detection, and diagnosis is frequently delayed. The challenges of research studies in this population include epidemiologic barriers to research such as small patient numbers, heterogeneity of cardiac disease, and coexistence of multiple risk factors. Based on stroke burden and study feasibility, studies involving mechanical circulatory support, single ventricle patients, early stroke detection strategies, and understanding secondary stroke risk factors and prevention are the highest research priorities over the next 5-10 years. The development of large-scale multicenter and multispecialty collaborative research is a critical next step. The designation of centers of expertise will assist in clinical care and research. Conclusions There is an urgent need for additional research to improve the quality of evidence in guideline recommendations for cardiogenic stroke in children. Although significant barriers to clinical research exist, multicenter and multispecialty collaboration is an important step toward advancing clinical care and research for children with cardiac disease and stroke. [ABSTRACT FROM AUTHOR]
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- 2015
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19. Analysis of the effect of estrogen/androgen perturbation on penile development in transgenic and diethylstilbestrol-Treated mice.
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Blaschko, Sarah D., Mahawong, Phitsanu, Ferretti, Max, Cunha, Tristan J., Sinclair, Adriane, Wang, Hong, Schlomer, Bruce J., Risbridger, Gail, Baskin, Laurence S., and Cunha, Gerald R.
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- 2013
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20. PD55-10 MACROSCOPIC WHOLE-MOUNTS CHARACTERIZING SEXUAL DIFFERENTIATION OF THE HUMAN FETAL UROGENITAL TRACT.
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Shen, Joel, Isaacson, Dylan S., Cao, Mei, Sinclair, Adriane W., Cunha, Gerald R., and Baskin, Laurence S.
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SEX differentiation (Embryology) ,GENITOURINARY organs ,SEX differentiation disorders - Published
- 2018
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21. Association Between Prolonged Seizures and Malignant Middle Cerebral Artery Infarction in Children With Acute Ischemic Stroke.
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Andrade, Andrea, Bigi, Sandra, Laughlin, Suzanne, Parthasarathy, Sujatha, Sinclair, Adriane, Dirks, Peter, Pontigon, Ann Marie, Moharir, Mahendranath, Askalan, Rand, MacGregor, Daune, and deVeber, Gabrielle
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CEREBRAL arterial diseases , *INFARCTION , *ISCHEMIA , *STROKE , *DECOMPRESSIVE craniectomy - Abstract
Background: Malignant middle cerebral artery infarct syndrome is a potentially fatal complication of stroke that is poorly understood in children. We studied the frequency, associated characteristics, and outcomes of this condition in children.Methods: Children, aged two months to 18 years with acute middle cerebral artery infarct diagnosed at our center between January 2005 and December 2012 were studied. Associations with malignant middle cerebral artery infarct syndrome were sought, including age, seizures, neurological deficit severity (Pediatric National Institute of Health Stroke Severity Score), stroke etiology, fever, blood pressure, blood glucose, infarct location, infarct volume (modified pediatric Alberta Stroke Program Early Computed Tomography Score), and arterial occlusion. Death and neurological outcomes were determined.Results: Among 66 children with middle cerebral artery stroke, 12 (18%) developed malignant middle cerebral artery infarct syndrome, fatal in three. Prolonged seizures during the first 24 hours (odds ratio, 25.51; 95% confidence interval, 3.10 to 334.81; P = 0.005) and a higher Pediatric National Institute of Health Stroke Severity Score (odds ratio, 1.22; 95% confidence interval, 1.08 to 1.45; P = 0.006) were independently associated with malignant middle cerebral artery infarct syndrome. All children aged greater than two years with a Pediatric National Institute of Health Stroke Severity Score ≥8 and initial seizures ≥5 minutes duration developed malignant middle cerebral artery infarct syndrome (100%).Conclusions: Malignant middle cerebral artery infarct syndrome affects nearly one in five children with acute middle cerebral artery stroke. Children with higher Pediatric National Institute of Health Stroke Severity Scores and prolonged initial seizures are at greatly increased risk for malignant middle cerebral artery infarct syndrome. Children with middle cerebral artery infarcts warrant intensive neuroprotective management and close monitoring to enable early referral for hemicraniectomy surgery. [ABSTRACT FROM AUTHOR]- Published
- 2016
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22. Estrogens and development of the mouse and human external genitalia.
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Baskin L, Sinclair A, Derpinghaus A, Cao M, Li Y, Overland M, Aksel S, and Cunha GR
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- Animals, Estrogens genetics, Female, Genitalia, Male metabolism, Humans, Male, Mice, Organogenesis genetics, Penis growth & development, Penis metabolism, Estrogen Receptor alpha genetics, Estrogen Receptor beta genetics, Estrogens metabolism, Genitalia, Male growth & development, Receptors, Androgen genetics
- Abstract
The Jost hypothesis states that androgens are necessary for normal development of the male external genitalia. In this review, we explore the complementary hypothesis that estrogens can elicit abnormal development of male external genitalia. Herein, we review available data in both humans and mice on the deleterious effects of estrogen on external genitalia development, especially during the "window of susceptibility" to exogenous estrogens. The male and female developing external genitalia in both the human and mouse express ESR1 and ESR2, along with the androgen receptor (AR). Human clinical data suggests that exogenous estrogens can adversely affect normal penile and urethral development, resulting in hypospadias. Experimental mouse data also strongly supports the idea that exogenous estrogens cause penile and urethral defects. Despite key differences, estrogen-induced hypospadias in the mouse displays certain morphogenetic homologies to human hypospadias, including disruption of urethral fusion and preputial abnormalities. Timing of estrogenic exposure, or the "window of susceptibility," is an important consideration when examining malformations of the external genitalia in both humans and mice. In addition to a review of normal human and mouse external genital development, this article aims to review the present data on the role of estrogens in normal and abnormal development of the mouse and human internal and external genitalia. Based on the current literature for both species, we conclude that estrogen-dependent processes may play a role in abnormal genital development., (Copyright © 2020 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2021
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23. Anatomy of the mouse penis and internal prepuce.
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Cunha GR, Cao M, Sinclair A, Derpinghaus A, and Baskin LS
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- Animals, Dissection, Epithelium anatomy & histology, Hypospadias pathology, Male, Mice, Mucous Membrane anatomy & histology, Penis anatomy & histology, Penis growth & development
- Abstract
This paper addresses a confusing issue of preputial anatomy of the mouse. The term "internal prepuce" was used in 2013 to describe a preputial structure integral to the mouse glans penis. Subsequently in 2015 the same term was applied by another group to describe entirely different morphology, generating confusion in the literature. Because it is inappropriate to use the same term to describe entirely different structures, we take this opportunity to provide further descriptive information on the internal prepuce of the mouse employing gross dissection, analysis of serial histologic section sets, three-dimensional reconstruction, scanning electron microscopy and immunohistochemistry. For this purpose, we review and illustrate the relevant literature and provide some additional new data using standard morphological techniques including immunohistochemistry. The mouse internal prepuce is integral to the glans penis and clearly is involved in sexual function in so far as it contains a major erectile body innervated by penile nerves. The development of the mouse internal prepuce is described for the first time and related to the development of the corpus cavernosum glandis., (Copyright © 2020 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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24. Chloride channels regulate differentiation and barrier functions of the mammalian airway.
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He M, Wu B, Ye W, Le DD, Sinclair AW, Padovano V, Chen Y, Li KX, Sit R, Tan M, Caplan MJ, Neff N, Jan YN, Darmanis S, and Jan LY
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- Animals, Cell Differentiation physiology, Humans, Mice, Organogenesis physiology, Respiratory Mucosa metabolism, Trachea embryology, Trachea metabolism, Anoctamin-1 metabolism, Neoplasm Proteins metabolism, Respiratory Mucosa embryology
- Abstract
The conducting airway forms a protective mucosal barrier and is the primary target of airway disorders. The molecular events required for the formation and function of the airway mucosal barrier, as well as the mechanisms by which barrier dysfunction leads to early onset airway diseases, remain unclear. In this study, we systematically characterized the developmental landscape of the mouse airway using single-cell RNA sequencing and identified remarkably conserved cellular programs operating during human fetal development. We demonstrated that in mouse, genetic inactivation of chloride channel Ano1/Tmem16a compromises airway barrier function, results in early signs of inflammation, and alters the airway cellular landscape by depleting epithelial progenitors. Mouse Ano1
-/- mutants exhibited mucus obstruction and abnormal mucociliary clearance that resemble the airway defects associated with cystic fibrosis. The data reveal critical and non-redundant roles for Ano1 in organogenesis, and show that chloride channels are essential for mammalian airway formation and function., Competing Interests: MH, BW, WY, DL, AS, VP, YC, KL, RS, MT, MC, NN, YJ, SD, LJ No competing interests declared, (© 2020, He et al.)- Published
- 2020
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25. Hot spots in fetal human penile and clitoral development.
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Baskin L, Derpinghaus A, Cao M, Sinclair A, Li Y, Overland M, and Cunha GR
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- Apoptosis genetics, Cell Proliferation genetics, Clitoris metabolism, Embryonic Development, Epithelium growth & development, Epithelium metabolism, Female, Fetus, Gene Expression Regulation, Developmental genetics, Genitalia, Female growth & development, Genitalia, Female metabolism, Humans, Male, Microscopy, Electron, Scanning, Penis metabolism, Urethra growth & development, Urethra metabolism, Caspase 3 genetics, Clitoris growth & development, Ki-67 Antigen genetics, Penis growth & development
- Abstract
To better understand how the human fetal penis and clitoris grows and remodels, we undertook an investigation to define active areas of cellular proliferation and programmed cell death spatially and temporally during development of human fetal external genitalia from the indifferent stage (8 weeks) to 18 weeks of gestation. Fifty normal human fetal penile and clitoral specimens were examined using macroscopic imaging, scanning electron microscopy and immunohistochemical localization for the cellular proliferation and apoptotic markers, Ki67 and Caspase-3, respectively. A number of hot spots of cellular proliferation characterized by Ki67 localization are present in the penis and clitoris especially early in development, most notably in the corporal body, glans, remodeling glanular urethra, the urethral plate, the roof of the urethral groove and the fully formed penile urethra. The 12-fold increase in penile length over 10 weeks of growth from 8 to 18 weeks of gestation based on Ki67 labelling appears to be driven by cellular proliferation in the corporal body and glans. Throughout all ages in both the developing penis and clitoris Ki67 labeling was consistently elevated in the ventral epidermis and ventral mesenchyme relative to the dorsal counterparts. This finding is consistent with the intense morphogenetic activity/remodeling in the ventral half of the genital tubercle in both sexes involving formation of the urethral/vestibular plates, canalization of the urethral/vestibular plates and fusion of the urethral folds to form the penile urethra. Areas of reduced or absent Ki67 staining include the urethral fold epithelium that fuses to form the penile tubular urethra. In contrast, the urethral fold mesenchyme is positive for Ki67. Apoptosis was rarely noted in the developing penis and clitoris; the only area of minimal Caspase-3 localization was in the epithelium of the ventral epithelial glanular channel remodeling., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2020
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26. Androgen-independent events in penile development in humans and animals.
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Cunha GR, Liu G, Sinclair A, Cao M, Glickman S, Cooke PS, and Baskin L
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- Animals, Humans, Male, Penis metabolism, Androgens metabolism, Organogenesis, Penis growth & development, Receptors, Androgen metabolism
- Abstract
The common view on penile development is that it is androgen-dependent, based first and foremost on the fact that the genital tubercle forms a penis in males and a clitoris in females. However, critical examination of the complex processes involved in human penile development reveals that many individual steps in development of the genital tubercle are common to both males and females, and thus can be interpreted as androgen-independent. For certain developmental events this conclusion is bolstered by observations in androgen-insensitive patients and androgen receptor mutant mice. Events in genital tubercle development that are common to human males and females include: formation of (a) the genital tubercle, (b) the urethral plate, (c) the urethral groove, (d) the glans, (e) the prepuce and (f) the corporal body. For humans 6 of 13 individual developmental steps in penile development were interpreted as androgen-independent. For mice 5 of 11 individual developmental steps were found to be androgen-independent, which were verified through analysis of androgen-insensitive mutants. Observations from development of external genitalia of other species (moles and spotted hyena) provide further examples of androgen-independent events in penile development. These observations support the counter-intuitive idea that penile development involves both androgen-independent and androgen-dependent processes., (Copyright © 2019 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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27. Androgen and estrogen receptor expression in the developing human penis and clitoris.
- Author
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Baskin L, Cao M, Sinclair A, Li Y, Overland M, Isaacson D, and Cunha GR
- Subjects
- Clitoris ultrastructure, Female, Humans, Male, Microscopy, Electron, Scanning, Penis ultrastructure, Clitoris embryology, Clitoris metabolism, Morphogenesis, Penis embryology, Penis metabolism, Receptors, Androgen metabolism, Receptors, Estrogen metabolism
- Abstract
To better understand how the human fetal penis and clitoris grows and remodels, we undertook an investigation to define active areas of cellular proliferation and programmed cell death spatially and temporally during development of human fetal external genitalia from the indifferent stage (8 weeks) to 18 weeks of gestation. Fifty normal human fetal penile and clitoral specimens were examined using macroscopic imaging, scanning electron microscopy and immunohistochemical localization for the cellular proliferation and apoptotic markers, Ki67 and Caspase-3. A number of hot spots of cellular proliferation characterized by Ki67 localization are present in the penis and clitoris especially early in development, most notably in the corporal body, glans, remodeling glanular urethra, the urethral plate, the roof of the urethral groove and the fully formed penile urethra. The 12-fold increase in penile length over 10 weeks of growth from 8 to 18 weeks of gestation based on Ki67 labelling appears to be driven by cellular proliferation in the corporal body and glans. Throughout all ages in both the developing penis and clitoris Ki67 labeling was consistently elevated in the ventral epidermis and ventral mesenchyme relative to the dorsal counterparts. This finding is consistent with the intense morphogenetic activity/remodeling in the ventral half of the genital tubercle in both sexes involving formation of the urethral/vestibular plates, canalization of the urethral/vestibular plates and fusion of the urethral folds to form the penile urethra. Areas of reduced or absent Ki67 staining include the urethral fold epithelium that fuses to form the penile tubular urethra. In contrast, the urethral fold mesenchyme is positive for Ki67. Apoptosis was rarely noted in the developing penis and clitoris; the only area of minimal Caspase-3 localization was in the epithelium of the ventral epithelial glanular channel remodeling., (Copyright © 2019 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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- View/download PDF
28. Imaging the developing human external and internal urogenital organs with light sheet fluorescence microscopy.
- Author
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Isaacson D, McCreedy D, Calvert M, Shen J, Sinclair A, Cao M, Li Y, McDevitt T, Cunha G, and Baskin L
- Subjects
- Humans, Image Processing, Computer-Assisted methods, Imaging, Three-Dimensional methods, Microscopy, Fluorescence methods, Specimen Handling methods, Urogenital System cytology
- Abstract
Technological advances in three-dimensional (3D) reconstruction techniques have previously enabled paradigm shifts in our understanding of human embryonic and fetal development. Light sheet fluorescence microscopy (LSFM) is a recently-developed technique that uses thin planes of light to optically section whole-mount cleared and immunolabeled biologic specimens. The advent of commercially-available light sheet microscopes has facilitated a new generation of research into protein localization and tissue dynamics at extremely high resolution. Our group has applied LSFM to study developing human fetal external genitalia, internal genitalia and kidneys. This review describes LSFM and presents our group's technique for preparing, clearing, immunostaining and imaging human fetal urogenital specimens. We then present light sheet images and videos of each element of the developing human urogenital system. To the extent of our knowledge, the work conducted by our laboratory represents the first description of a method for performing LSFM on the full human urogenital system during the embryonic and fetal periods., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2020
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29. Development of the human prepuce and its innervation.
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Cunha GR, Sinclair A, Cao M, and Baskin LS
- Subjects
- Humans, Male, Microscopy, Electron, Scanning, Penis innervation, Penis metabolism, Penis ultrastructure, Urethra innervation, Urethra metabolism, Urethra ultrastructure, Morphogenesis, Penis growth & development, Receptors, Androgen metabolism, Receptors, Estrogen metabolism, Urethra growth & development
- Abstract
Development of the human prepuce was studied over the course of 9-17 weeks of gestation in 30 specimens. Scanning electron microscopy revealed subtle surface features that were associated with preputial development, namely the appearance of epidermal aggregates that appeared to be associated with formation of the preputial fold. Transverse and sagittal sections revealed that the epidermis of the glans is considerably thicker than that of the penile shaft. We described a novel morphogenetic mechanism of formation of the preputial lamina, namely the splitting of the thick epidermis of the glans into the preputial lamina and the epidermis via the intrusion of mesenchyme containing red blood cells and CD31-positive blood vessels. This process begins at 10-11 weeks of gestation in the proximal aspect of the glans and extends distally. The process is likely to be androgen-dependent and mediated via androgen receptors strategically localized to the morphogenetic process, but signaling through estrogen receptor may play a role. Estrogen receptor alpha (ESR1) has a very limited expression in the developing human glans and prepuce, while estrogen receptor beta (ESR2) is expressed more broadly in the developing preputial lamina, epidermis and urethra. Examination of the ontogeny of innervation of the glans penis and prepuce reveals the presence of the dorsal nerve of the penis as early as 9 weeks of gestation. Nerve fibers enter the glans penis proximally and extend distally over several weeks to eventually reach the distal aspect of the glans and prepuce by 14-16 weeks of gestation., (Copyright © 2019 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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- View/download PDF
30. Clitoral development in the mouse and human.
- Author
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Cunha GR, Liu G, Sinclair A, Cao M, and Baskin L
- Subjects
- Animals, Female, Humans, Mice, Clitoris growth & development, Clitoris ultrastructure, Microscopy, Electron, Scanning methods
- Abstract
The goal of this report is (a) to provide the first detailed description of mouse clitoral development, and (b) to compare mouse and human clitoral development. For this purpose, external genitalia of female mice were examined by wholemount microscopy, histology and immunohistochemistry from 14 days of gestation to 10 days postnatal. Human clitoral development was examined by these techniques as well as by scanning electron microscopy and optical projection tomography from 8 to 19 weeks of gestation. The adult mouse clitoris is an internal organ defined by a U-shaped clitoral lamina whose development is associated with the prenatal medial and distal growth of the female preputial swellings along the sides of the genital tubercle to form the circumferential preputial lamina. Regression of the ventral aspect of the preputial lamina leads to formation of the U-shaped clitoral lamina recognized as early as 17 days of gestation. While the adult U-shaped mouse clitoral lamina is closely associated with the vagina, and it appears to be completely non-responsive to estrogen as opposed to the highly estrogen-responsive vaginal epithelium. The prominent perineal appendage in adult females is prepuce, formed via fusion of the embryonic preputial swellings and is not the clitoris. The human clitoris is in many respects a smaller anatomic version of the human penis having all of the external and internal elements except the urethra. The human clitoris (like the human penis) is derived from the genital tubercle with the clitoral glans projecting into the vaginal vestibule. Adult morphology and developmental processes are virtually non-comparable in the mouse and human clitoris., (Copyright © 2019 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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31. Reproductive tract biology: Of mice and men.
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Cunha GR, Sinclair A, Ricke WA, Robboy SJ, Cao M, and Baskin LS
- Subjects
- Animals, Female, Gene Expression Regulation, Developmental physiology, Humans, Mice, Organogenesis physiology, Epithelium growth & development, Genitalia, Female growth & development, Mullerian Ducts growth & development, Uterus growth & development
- Abstract
The study of male and female reproductive tract development requires expertise in two separate disciplines, developmental biology and endocrinology. For ease of experimentation and economy, the mouse has been used extensively as a model for human development and pathogenesis, and for the most part similarities in developmental processes and hormone action provide ample justification for the relevance of mouse models for human reproductive tract development. Indeed, there are many examples describing the phenotype of human genetic disorders that have a reasonably comparable phenotype in mice, attesting to the congruence between mouse and human development. However, anatomic, developmental and endocrinologic differences exist between mice and humans that (1) must be appreciated and (2) considered with caution when extrapolating information between all animal models and humans. It is critical that the investigator be aware of both the similarities and differences in organogenesis and hormone action within male and female reproductive tracts so as to focus on those features of mouse models with clear relevance to human development/pathology. This review, written by a team with extensive expertise in the anatomy, developmental biology and endocrinology of both mouse and human urogenital tracts, focusses upon the significant human/mouse differences, and when appropriate voices a cautionary note regarding extrapolation of mouse models for understanding development of human male and female reproductive tracts., (Copyright © 2019 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2019
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32. Development of the human bladder and ureterovesical junction.
- Author
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Liaw A, Cunha GR, Shen J, Cao M, Liu G, Sinclair A, and Baskin L
- Subjects
- Animals, Female, Gene Expression Regulation, Developmental, Humans, Male, Urogenital System growth & development, Embryonic Development genetics, Kidney growth & development, Urinary Bladder growth & development, Wolffian Ducts growth & development
- Abstract
The urinary bladder collects urine from the kidneys and stores it until the appropriate moment for voiding. The trigone and ureterovesical junctions are key to bladder function, by allowing one-way passage of urine into the bladder without obstruction. Embryological development of these structures has been studied in multiple animal models as well as humans. In this report we review the existing literature on bladder development and cellular signalling with particular focus on bladder development in humans. The bladder and ureterovesical junction form primarily during the fourth to eighth weeks of gestation, and arise from the primitive urogenital sinus following subdivision of the cloaca. The bladder develops through mesenchymal-epithelial interactions between the endoderm of the urogenital sinus and mesodermal mesenchyme. Key signalling factors in bladder development include shh, TGF-β, Bmp4, and Fgfr2. A concentration gradient of shh is particularly important in development of bladder musculature, which is vital to bladder function. The ureterovesical junction forms from the interaction between the Wolffian duct and the bladder. The ureteric bud arises from the Wolffian duct and is incorporated into the developing bladder at the trigone. It was previously thought that the trigonal musculature developed primarily from the Wolffian duct, but it has been shown to develop primarily from bladder mesenchyme. Following emergence of the ureters from the Wolffian ducts, extensive epithelial remodelling brings the ureters to their final trigonal positions via vitamin A-induced apoptosis. Perturbation of this process is implicated in clinical obstruction or urine reflux. Congenital malformations include ureteric duplication and bladder exstrophy., (Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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33. Human glans and preputial development.
- Author
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Liu X, Liu G, Shen J, Yue A, Isaacson D, Sinclair A, Cao M, Liaw A, Cunha GR, and Baskin L
- Subjects
- Endoderm growth & development, Endoderm metabolism, Endoderm ultrastructure, Epithelial Cells metabolism, Epithelial Cells ultrastructure, Gene Expression Regulation, Developmental genetics, Hepatocyte Nuclear Factor 3-alpha genetics, Humans, Male, Penis growth & development, Receptors, Androgen genetics, Urethra growth & development, Uroplakins genetics, Cell Differentiation genetics, Morphogenesis genetics, Penis ultrastructure, Urethra ultrastructure
- Abstract
The urethra within the human penile shaft develops via (1) an "Opening Zipper" that facilitates distal canalization of the solid urethral plate to form a wide urethral groove and (2) a "Closing Zipper" that facilitates fusion of the epithelial surfaces of the urethral folds. Herein, we extend our knowledge by describing formation of the human urethra within the glans penis as well as development of the prepuce. Forty-eight normal human fetal penile specimens were examined using scanning electron microscopy and optical projection tomography. Serial histologic sections were evaluated for morphology and immunohistochemical localization for epithelial differentiation markers: Cytokeratins 6, 7, 10, FoxA1, uroplakin and the androgen receptor. As the closing zipper completes fusion of the urethral folds within the penile shaft to form a tubular urethra (~ 13 weeks), canalization of the urethral plate continues in proximal to distal fashion into the glans penis to directly form the urethra within the glans without forming an open urethral groove. Initially, the urethral plate is attached ventrally to the epidermis via an epithelial seam, which is remodeled and eliminated, thus establishing mesenchymal confluence ventral to the glanular urethra. The morphogenetic remodeling involves the strategic expression of cytokeratin 7, FoxA1 and uroplakin in endodermal epithelial cells as the tubular glanular urethra forms. The most ventral epithelial cells of the urethral plate are pinched off from the glanular urethra and are reabsorbed into the epidermis ultimately losing expression of their markers, a process undoubtedly regulated by androgens. The prepuce initially forms on the dorsal aspect of the glans at approximately 12 weeks of gestation. After sequential proximal to distal remodeling of the ventral urethral plate along the ventral aspect of glans, the prepuce of epidermal origin fuses in the ventral midline., (Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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- View/download PDF
34. Development of the human penis and clitoris.
- Author
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Baskin L, Shen J, Sinclair A, Cao M, Liu X, Liu G, Isaacson D, Overland M, Li Y, and Cunha GR
- Subjects
- Clitoris ultrastructure, Female, Humans, Male, Penis ultrastructure, Urethra ultrastructure, Clitoris growth & development, Microscopy, Electron, Scanning methods, Penis growth & development, Urethra growth & development
- Abstract
The human penis and clitoris develop from the ambisexual genital tubercle. To compare and contrast the development of human penis and clitoris, we used macroscopic photography, optical projection tomography, light sheet microscopy, scanning electron microscopy, histology and immunohistochemistry. The human genital tubercle differentiates into a penis under the influence of androgens forming a tubular urethra that develops by canalization of the urethral plate to form a wide diamond-shaped urethral groove (opening zipper) whose edges (urethral folds) fuse in the midline (closing zipper). In contrast, in females, without the influence of androgens, the vestibular plate (homologue of the urethral plate) undergoes canalization to form a wide vestibular groove whose edges (vestibular folds) remain unfused, ultimately forming the labia minora defining the vaginal vestibule. The neurovascular anatomy is similar in both the developing human penis and clitoris and is the key to successful surgical reconstructions., (Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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- View/download PDF
35. Immunohistochemical expression analysis of the human fetal lower urogenital tract.
- Author
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Shen J, Isaacson D, Cao M, Sinclair A, Cunha GR, and Baskin L
- Subjects
- Clitoris growth & development, Clitoris metabolism, Epithelium growth & development, Epithelium metabolism, Female, Gene Expression Regulation, Developmental, Genitalia, Female growth & development, Hepatocyte Nuclear Factor 3-alpha genetics, Humans, Keratin-10 genetics, Male, PAX2 Transcription Factor genetics, Penis growth & development, Penis metabolism, Urethra metabolism, Urogenital System metabolism, Vagina growth & development, Vagina metabolism, Fetal Development genetics, Immunohistochemistry, Urethra growth & development, Urogenital System growth & development
- Abstract
We have studied the ontogeny of the developing human male and female urogenital tracts from 9 weeks (indifferent stage) to 16 weeks (advanced sex differentiation) of gestation by immunohistochemistry on mid-sagittal sections. Sixteen human fetal pelvises were serial sectioned in the sagittal plane and stained with antibodies to epithelial, muscle, nerve, proliferation and hormone receptor markers. Key findings are: (1) The corpus cavernosum in males and females extends into the glans penis and clitoris, respectively, during the ambisexual stage (9 weeks) and thus appears to be an androgen-independent event. (2) The entire human male (and female) urethra is endodermal in origin based on the presence of FOXA1, KRT 7, uroplakin, and the absence of KRT10 staining. The endoderm of the urethra interfaces with ectodermal epidermis at the site of the urethral meatus. (3) The surface epithelium of the verumontanum is endodermal in origin (FOXA1-positive) with a possible contribution of Pax2-positive epithelial cells implying additional input from the Wolffian duct epithelium. (4) Prostatic ducts arise from the endodermal (FOXA1-positive) urogenital sinus epithelium near the verumontanum. (5) Immunohistochemical staining of mid-sagittal and para-sagittal sections revealed the external anal sphincter, levator ani, bulbospongiosus muscle and the anatomic relationships between these developing skeletal muscles and organs of the male and female reproductive tracts. Future studies of normal human developmental anatomy will lay the foundation for understanding congenital anomalies of the lower urogenital tract., (Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
36. Three-dimensional imaging of the developing human fetal urogenital-genital tract: Indifferent stage to male and female differentiation.
- Author
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Isaacson D, Shen J, Overland M, Li Y, Sinclair A, Cao M, McCreedy D, Calvert M, McDevitt T, Cunha GR, and Baskin L
- Subjects
- Female, Humans, Male, Microscopy, Electron, Scanning, Urogenital System growth & development, Fetal Development genetics, Imaging, Three-Dimensional methods, Sex Differentiation genetics, Urogenital System ultrastructure
- Abstract
Recent studies in our lab have utilized three imaging techniques to visualize the developing human fetal urogenital tract in three dimensions: optical projection tomography, scanning electron microscopy and lightsheet fluorescence microscopy. We have applied these technologies to examine changes in morphology and differential gene expression in developing human external genital specimens from the ambisexual stage (<9 weeks fetal age) to well-differentiated male and female organs (>13 weeks fetal age). This work outlines the history and function of each of these three imaging modalities, our methods to prepare specimens for each and the novel findings we have produced thus far. We believe the images in this paper of human fetal urogenital organs produced using lightsheet fluorescence microscopy are the first published to date., (Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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37. Macroscopic whole-mounts of the developing human fetal urogenital-genital tract: Indifferent stage to male and female differentiation.
- Author
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Shen J, Cunha GR, Sinclair A, Cao M, Isaacson D, and Baskin L
- Subjects
- Female, Fetal Development, Fetus, Genitalia embryology, Genitalia growth & development, Genitalia ultrastructure, Humans, Male, Ovary embryology, Ovary growth & development, Testis embryology, Testis growth & development, Urogenital System growth & development, Cell Differentiation genetics, Ovary ultrastructure, Testis ultrastructure, Urogenital System ultrastructure
- Abstract
We present a detailed review of fetal development of the male and female human urogenital tract from 8 to 22 weeks gestation at the macroscopic and morphometric levels. Human fetal specimens were sexed based on macroscopic identification of fetal testes or ovaries, Wolffian or Müllerian structures and the presence of the SRY gene in the specimens at or near the indifferent stage (8-9 weeks). Specimens were photographed using a dissecting microscope with transmitted and reflected light. Morphometric measurements were taken of each urogenital organ. During this time period, development of the male and female urogenital tracts proceeded from the indifferent stage to differentiated organs. The kidneys, ureters, and bladder developed identically, irrespective of sex with the same physical dimensions and morphologic appearance. The penis, prostate and testis developed in males and the clitoris, uterus and ovary in females. Androgen-dependent growth certainly influenced size and morphology of the penile urethra and prostate, however, androgen-independent growth also accounted for substantial growth in the fetal urogenital tract including the clitoris., (Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
38. Contrasting mechanisms of penile urethral formation in mouse and human.
- Author
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Liu G, Liu X, Shen J, Sinclair A, Baskin L, and Cunha GR
- Subjects
- Animals, Endoderm growth & development, Female, Genitalia, Female growth & development, Humans, Male, Hypospadias metabolism, Penis embryology, Urethra embryology
- Abstract
This paper addresses the developmental mechanisms of formation of the mouse and human penile urethra and the possibility that two disparate mechanisms are at play. It has been suggested that the entire penile urethra of the mouse forms via direct canalization of the endodermal urethral plate. While this mechanism surely accounts for development of the proximal portion of the mouse penile urethra, we suggest that the distal portion of the mouse penile urethra forms via a series of epithelial fusion events. Through review of the recent literature in combination with new data, it is unlikely that the entire mouse urethra is formed from the endodermal urethral plate due in part to the fact that from E14 onward the urethral plate is not present in the distal aspect of the genital tubercle. Formation of the distal portion of the mouse urethra receives substantial contribution from the preputial swellings that form the preputial-urethral groove and subsequently the preputial-urethral canal, the later of which is subdivided by a fusion event to form the distal portion of the mouse penile urethra. Examination of human penile development also reveals comparable dual morphogenetic mechanisms. However, in the case of human, direct canalization of the urethral plate occurs in the glans, while fusion events are involved in formation of the urethra within the penile shaft, a pattern exactly opposite to that of the mouse. The highest incidence of hypospadias in humans occurs at the junction of these two different developmental mechanisms. The relevance of the mouse as a model of human hypospadias is discussed., (Copyright © 2018 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
39. Renal Subcapsular xenografing of human fetal external genital tissue - A new model for investigating urethral development.
- Author
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Isaacson D, Shen J, Cao M, Sinclair A, Yue X, Cunha G, and Baskin L
- Subjects
- Animals, Endocrine Disruptors pharmacology, Female, Genitalia, Female growth & development, Humans, Hypospadias drug therapy, Kidney embryology, Male, Mice, Receptors, Androgen metabolism, Hypospadias pathology, Organogenesis physiology, Penis embryology, Urethra drug effects
- Abstract
In this paper, we introduce our novel renal subcapsular xenograft model for the study of human penile urethral and clitoral development. We grafted fifteen intact fetal penes and clitorides 8-11 weeks fetal age under the renal capsules of gonadectomized athymic mice. The mice were treated with a subcutaneous pellet of dihydrotestosterone (DHT), diethylstilbestrol (DES) or untreated with hormones. Xenografts were harvested after fourteen days of growth and analyzed via serial histologic sectioning and immunostaining for Ki-67, cytokeratins 6, 7 and 10, uroplakin and the androgen receptor. Non-grafted specimens of similar fetal age were sectioned and immunostained for the same antigenic markers. 14/15 (93.3%) grafts were successfully propagated and harvested. The developing urethral plate, urethral groove, tubular urethra, corporal bodies and preputial lamina were easily identifiable. These structures demonstrated robust cellularity, appropriate architecture and abundant Ki-67 expression. Expression patterns of cytokeratins 6, 7 and 10, uroplakin and the androgen receptor in xenografted specimens demonstrated characteristic male/female differences analogous to non-grafted specimens. DHT treatment reliably produced tubularization of nascent urethral and vestibular structures and male patterns of androgen receptor expression in grafts of both genetic sexes while estrogenic or hormonally absent conditions reliably resulted in a persistent open urethral/vestibular groove and female patterns of androgen receptor expression. This model's success enables further study into causal pathways by which endocrine-disrupting and endocrine-mimicking substances may directly cause disruption of normal human urethral development or hypospadias., (Copyright © 2017 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
40. Flutamide-induced hypospadias in rats: A critical assessment.
- Author
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Sinclair AW, Cao M, Pask A, Baskin L, and Cunha GR
- Subjects
- Animals, Epithelium ultrastructure, Flutamide toxicity, Hypospadias chemically induced, Male, Penis growth & development, Penis ultrastructure, Rats, Urethra ultrastructure, Epithelium physiopathology, Hypospadias physiopathology, Penis physiopathology, Urethra physiopathology
- Abstract
This paper provides the first detailed description of flutamide-induced hypospadias in the rat based upon wholemount, histologic, three-dimensional reconstruction, scanning electron microscopic, and immunocytochemical analysis. The penile malformations elicited by this potent anti-androgen include a substantial proximal shift in the urethral meatus that clearly conforms to the definition of hypospadias based upon specific morphological criteria for this malformation. Through examination of the normal penile development and flutamide-induced abnormal penile development observed in prenatally oil- and flutamide-treated rats, our analysis provides insights into the morphogenetic mechanism of development of hypospadias. In this regard, a common theme in normal penile development is midline fusion of epithelia followed by removal of the epithelial seam and establishment of midline mesenchymal confluence during development of the penile urethra and prepuce, processes which are impaired as a result of prenatal flutamide treatment. The developmental processes occurring in normal penile development, through comparison with development of female external genitalia and those impaired due to prenatal flutamide treatment, are consistent with critical role of androgen receptors in normal penile development in the rat, and the specific penile abnormalities embodied in flutamide-induced rat hypospadias., (Copyright © 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
41. Mouse hypospadias: A critical examination and definition.
- Author
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Sinclair AW, Cao M, Shen J, Cooke P, Risbridger G, Baskin L, and Cunha GR
- Subjects
- Animals, Diethylstilbestrol toxicity, Humans, Hypospadias chemically induced, Male, Mice, Penis embryology, Urethra drug effects, Urethra physiopathology, Embryonic Development, Hypospadias physiopathology, Morphogenesis, Penis physiopathology
- Abstract
Hypospadias is a common malformation whose etiology is based upon perturbation of normal penile development. The mouse has been previously used as a model of hypospadias, despite an unacceptably wide range of definitions for this malformation. The current paper presents objective criteria and a definition of mouse hypospadias. Accordingly, diethylstilbestrol (DES) induced penile malformations were examined at 60 days postnatal (P60) in mice treated with DES over the age range of 12 days embryonic to 20 days postnatal (E12-P20). DES-induced hypospadias involves malformation of the urethral meatus, which is most severe in DES E12-P10, DES P0-P10 and DES P5-P15 groups, and less so or absent in the other treatment groups. A frenulum-like ventral tether between the penis and the prepuce was seen in the most severely affected DES-treated mice. Internal penile morphology was also altered in the DES E12-P10, DES P0-P10 and DES P5-P15 groups (with little effect in the other DES treatment groups). Thus, adverse effects of DES are a function of the period of DES treatment and most severe in the P0-P10 period. In "estrogen mutant mice" (NERKI, βERKO, αERKO and AROM+) hypospadias was only seen in AROM+ male mice having genetically-engineered elevation is serum estrogen. Significantly, mouse hypospadias was only seen distally at and near the urethral meatus where epithelial fusion events are known to take place and never in the penile midshaft, where urethral formation occurs via an entirely different morphogenetic process., (Copyright © 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
42. Complex epithelial remodeling underlie the fusion event in early fetal development of the human penile urethra.
- Author
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Shen J, Overland M, Sinclair A, Cao M, Yue X, Cunha G, and Baskin L
- Subjects
- Clitoris growth & development, Clitoris ultrastructure, Epithelium growth & development, Female, Genitalia, Female growth & development, Genitalia, Female ultrastructure, Humans, Hypospadias physiopathology, Male, Microscopy, Electron, Scanning, Penis growth & development, Urethra growth & development, Epithelium ultrastructure, Fetal Development, Penis ultrastructure, Urethra ultrastructure
- Abstract
We recently described a two-step process of urethral plate canalization and urethral fold fusion to form the human penile urethra. Canalization ("opening zipper") opens the solid urethral plate into a groove, and fusion ("closing zipper") closes the urethral groove to form the penile urethra. We hypothesize that failure of canalization and/or fusion during human urethral formation can lead to hypospadias. Herein, we use scanning electron microscopy (SEM) and analysis of transverse serial sections to better characterize development of the human fetal penile urethra as contrasted to the development of the human fetal clitoris. Eighteen 7-13 week human fetal external genitalia specimens were analyzed by SEM, and fifteen additional human fetal specimens were sectioned for histologic analysis. SEM images demonstrate canalization of the urethral/vestibular plate in the developing male and female external genitalia, respectively, followed by proximal to distal fusion of the urethral folds in males only. The fusion process during penile development occurs sequentially in multiple layers and through the interlacing of epidermal "cords". Complex epithelial organization is also noted at the site of active canalization. The demarcation between the epidermis of the shaft and the glans becomes distinct during development, and the epithelial tag at the distal tip of the penile and clitoral glans regresses as development progresses. In summary, SEM analysis of human fetal specimens supports the two-zipper hypothesis of formation of the penile urethra. The opening zipper progresses from proximal to distal along the shaft of the penis and clitoris into the glans in identical fashion in both sexes. The closing zipper mechanism is active only in males and is not a single process but rather a series of layered fusion events, uniquely different from the simple fusion of two epithelial surfaces as occurs in formation of the palate and neural tube., (Copyright © 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
43. Methods for studying human organogenesis.
- Author
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Cunha G, Overland M, Li Y, Cao M, Shen J, Sinclair A, and Baskin L
- Subjects
- Animals, Ecotoxicology, Gene Expression Regulation, Developmental, Humans, Mice, Aborted Fetus transplantation, Cell Differentiation genetics, Organogenesis genetics
- Abstract
This review details methods for utilizing D & C suction abortus specimens as a source of human fetal organs to study the morphogenetic and molecular mechanisms of human fetal organ development. By this means it is possible to design experiments elucidating the molecular mechanisms of human fetal organ development and to compare and contrast human developmental mechanisms with that of laboratory animals. Finally human fetal organs can be grown in vivo as grafts to athymic mice, thus allowing ethical analysis of potential adverse effects of environmental toxicants., (Copyright © 2015 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
44. Anatomy of mole external genitalia: Setting the record straight.
- Author
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Sinclair AW, Glickman SE, Baskin L, and Cunha GR
- Subjects
- Animals, Female, Male, Mice, Rats, Genitalia, Female anatomy & histology, Genitalia, Male anatomy & histology, Moles anatomy & histology
- Abstract
Anatomy of male and female external genitalia of adult mice (Mus musculus) and broad-footed moles (Scapanus latimanus) was re-examined to provide more meaningful anatomical terminology. In the past the perineal appendage of male broad-footed moles has been called the penis, while the female perineal appendage has been given several terms (e.g. clitoris, penile clitoris, peniform clitoris and others). Histological examination demonstrates that perineal appendages of male and female broad-footed moles are the prepuce, which in both sexes are covered externally with a hair-bearing epidermis and lacks erectile bodies. The inner preputial epithelium is non-hair-bearing and defines the preputial space in both sexes. The penis of broad-footed moles lies deep within the preputial space, is an "internal organ" in the resting state and contains the penile urethra, os penis, and erectile bodies. The clitoris of broad-footed moles is defined by a U-shaped clitoral epithelial lamina. Residing within clitoral stroma encompassed by the clitoral epithelial lamina is the corpus cavernosum, blood-filled spaces and the urethra. External genitalia of male and female mice are anatomically similar to that of broad-footed moles with the exception that in female mice the clitoris contains a small os clitoridis and lacks defined erectile bodies, while male mice have an os penis and a prominent distal cartilaginous structure within the male urogenital mating protuberance (MUMP). Clitori of female broad-footed moles lack an os clitoridis but contain defined erectile bodies, while male moles have an os penis similar to the mouse but lack the distal cartilaginous structure., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
- Full Text
- View/download PDF
45. Diethylstilbestrol-induced mouse hypospadias: "window of susceptibility".
- Author
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Sinclair AW, Cao M, Baskin L, and Cunha GR
- Subjects
- Animals, Animals, Newborn, Diethylstilbestrol toxicity, Disease Susceptibility chemically induced, Estrogens, Non-Steroidal, Female, Humans, Hypospadias chemically induced, Male, Mice, Penis growth & development, Pregnancy, Disease Susceptibility pathology, Hypospadias physiopathology, Morphogenesis drug effects, Penis pathology
- Abstract
This review presents published and novel results that define the programming window for diethylstilbestrol (DES)-induced abnormal development of the mouse penis. These data indicate that DES has its greatest effect during the period of most intense penile morphogenesis, namely postnatal days 0-15 (P0-P15). Pregnant mice and their neonatal pups were injected subcutaneously with 200 ng/gbw DES every other day from embryonic day 12-18 (DES E12-E18), postnatal day 0-10 (DES P0-P10), embryonic day 12 to postnatal day 10 (DES E12-P10), postnatal day 5-15 (DES P5-P15), and postnatal day 10-20 (DES P10-P20). Aged-matched controls received sesame oil vehicle. After euthanasia at 10, 15, 20 and 60 days, penises were analyzed by gross morphology, histology and morphometry. Penises of all 5 groups of DES-treated mice were reduced in size, which was confirmed by morphometric analysis of internal penile structures. The most profound effects were seen in the DES E12-P10, DES P0-P10, and DES P5-P15 groups, thus defining a DES "programming window". For all parameters, DES treatment from P10 to P20 showed the most mild of effects. Adverse effects of DES on the MUMP cartilage and erectile bodies observed shortly after the last DES injection reverted to normality in the DES P5-P15, but not in the E12-P10 and P0-P10 groups, in which MUMP cartilage and erectile body malformations persisted into adulthood, again emphasizing a "window of susceptibility" in the early neonatal period., (Copyright © 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
46. Comparative effects of neonatal diethylstilbestrol on external genitalia development in adult males of two mouse strains with differential estrogen sensitivity.
- Author
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Mahawong P, Sinclair A, Li Y, Schlomer B, Rodriguez E Jr, Ferretti MM, Liu B, Baskin LS, and Cunha GR
- Subjects
- Abnormalities, Drug-Induced etiology, Abnormalities, Drug-Induced metabolism, Animals, Female, Genitalia, Male drug effects, Genitalia, Male metabolism, Male, Mice, Mice, Inbred C57BL, Pregnancy, Receptors, Estrogen metabolism, Species Specificity, Abnormalities, Drug-Induced pathology, Diethylstilbestrol toxicity, Estrogens metabolism, Genitalia, Male abnormalities, Receptors, Estrogen genetics
- Abstract
The effect of neonatal exposure to diethylstilbestrol (DES), a potent synthetic estrogen, was examined to evaluate whether the CD-1 (estrogen insensitive, outbred) and C57 (estrogen sensitive, inbred) mouse strains differ in their response to estrogen disruption of male ExG differentiation. CD-1 and C57BL/6 litters were injected with sesame oil or DES (200 ng/g/5 μl in sesame oil vehicle) every other day from birth to day 10. Animals were sacrificed at the following time points: birth, 5, 10 and 60 days postnatal. Neonatally DES-treated mice from both strains had many ExG abnormalities that included the following: (a) severe truncation of the prepuce and glans penis, (b) an abnormal urethral meatus, (c) ventral tethering of the penis, (d) reduced os penis length and glans width, (e) impaired differentiation of cartilage, (f) absence of urethral flaps, and (g) impaired differentiation of erectile bodies. Adverse effects of DES correlated with the expression of estrogen receptors within the affected tissues. While the effects of DES were similar in the more estrogen-sensitive C57BL/6 mice versus the less estrogen-sensitive CD-1 mice, the severity of DES effects was consistently greater in C57BL/6 mice. We suggest that many of the effects of DES, including the induction of hypospadias, are due to impaired growth and tissue fusion events during development., (Copyright © 2014 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
47. Prenatal diethylstilbestrol induces malformation of the external genitalia of male and female mice and persistent second-generation developmental abnormalities of the external genitalia in two mouse strains.
- Author
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Mahawong P, Sinclair A, Li Y, Schlomer B, Rodriguez E Jr, Ferretti MM, Liu B, Baskin LS, and Cunha GR
- Subjects
- Abnormalities, Drug-Induced etiology, Abnormalities, Drug-Induced physiopathology, Animals, Female, Male, Mice, Mice, Inbred C57BL, Pregnancy, Abnormalities, Drug-Induced pathology, Diethylstilbestrol toxicity, Genitalia, Female abnormalities, Genitalia, Male abnormalities, Prenatal Exposure Delayed Effects
- Abstract
Potential trans-generational influence of diethylstilbestrol (DES) exposure emerged with reports of effects in grandchildren of DES-treated pregnant women and of reproductive tract tumors in offspring of mice exposed in utero to DES. Accordingly, we examined the trans-generational influence of DES on development of external genitalia (ExG) and compared effects of in utero DES exposure in CD-1 and C57BL/6 mice injected with oil or DES every other day from gestational days 12 to 18. Mice were examined at birth, and on 5-120 days postnatal to evaluate ExG malformations. Of 23 adult (>60 days) prenatally DES-exposed males, features indicative of urethral meatal hypospadias (see text for definitions) ranged from 18% to 100% in prenatally DES-exposed CD-1 males and 31% to 100% in prenatally DES-exposed C57BL/6 males. Thus, the strains differed only slightly in the incidence of male urethral hypospadias. Ninety-one percent of DES-exposed CD-1 females and 100% of DES-exposed C57BL/6 females had urethral-vaginal fistula. All DES-exposed CD-1 and C57BL/6 females lacked an os clitoris. None of the prenatally oil-treated CD-1 and C57BL/6 male and female mice had ExG malformations. For the second-generation study, 10 adult CD-1 males and females, from oil- and DES-exposed groups, respectively, were paired with untreated CD-1 mice for 30 days, and their offspring evaluated for ExG malformations. None of the F1 DES-treated females were fertile. Nine of 10 prenatally DES-exposed CD-1 males sired offspring with untreated females, producing 55 male and 42 female pups. Of the F2 DES-lineage adult males, 20% had exposed urethral flaps, a criterion of urethral meatal hypospadias. Five of 42 (11.9%) F2 DES lineage females had urethral-vaginal fistula. In contrast, all F2 oil-lineage males and all oil-lineage females were normal. Thus, prenatal DES exposure induces malformations of ExG in both sexes and strains of mice, and certain malformations are transmitted to the second-generation., (Copyright © 2014 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
48. Analysis of the effect of estrogen/androgen perturbation on penile development in transgenic and diethylstilbestrol-treated mice.
- Author
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Blaschko SD, Mahawong P, Ferretti M, Cunha TJ, Sinclair A, Wang H, Schlomer BJ, Risbridger G, Baskin LS, and Cunha GR
- Subjects
- Age Factors, Animals, Animals, Newborn, Aromatase deficiency, Aromatase genetics, Estrogen Receptor alpha deficiency, Estrogen Receptor alpha genetics, Estrogen Receptor beta deficiency, Estrogen Receptor beta genetics, Immunohistochemistry, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Microscopy, Electron, Scanning, Morphogenesis drug effects, Penis abnormalities, Penis metabolism, Signal Transduction drug effects, Aromatase metabolism, Diethylstilbestrol toxicity, Estrogen Receptor alpha metabolism, Estrogen Receptor beta metabolism, Estrogens, Non-Steroidal toxicity, Penis drug effects
- Abstract
Because both androgens and estrogens have been implicated in penile morphogenesis, we evaluated penile morphology in transgenic mice with known imbalance of androgen and estrogen signaling using scanning electron microscopy (SEM), histology, and immunohistochemistry of androgen and estrogen receptors α/β. Penises of adult wild-type, estrogen receptor-α knockout (αERKO), estrogen receptor-β knockout (βERKO), aromatase knockout (Arom-KO), and aromatase overexpression (Arom+) mice were evaluated, as well as adult mice treated with diethylstilbestrol (DES) from birth to day 10. Adult penises were examined because the adult pattern is the endpoint of development. The urethral orifice is formed by fusion of the MUMP (male urogenital mating protuberance) with the MUMP ridge, which consists of several processes fused to each other and to the MUMP. Similarly, the internal prepuce is completed ventrally by fusion of a ventral cleft. In adult murine penises the stromal processes that form the MUMP ridge are separated from their neighbors by clefts. αERKO, βERKO, and Arom-KO mice have penises with a MUMP ridge clefting pattern similar to that of wild-type mice. In contrast, Arom+ mice and neonatally DES-treated mice exhibit profound malformations of the MUMP, MUMP ridge clefting pattern, and internal prepuce. Abnormalities observed in Arom+ and neonatally DES-treated mice correlate with the expression of estrogen receptor-beta (ERβ) in the affected structures. This study demonstrates that formation of the urethal orifice and internal prepuce is due to fusion of separate epithelial-surfaced mesenchymal elements, a process dependent upon both androgen and estrogen signaling, in which ERβ signaling is strongly implicated., (Copyright © 2013 Wiley Periodicals, Inc.)
- Published
- 2013
- Full Text
- View/download PDF
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