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202 results on '"Shoda, K."'

5. Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression

Author

Shoda K, Asako Sakaue-Sawano, Atsushi Miyawaki, and Ryoko Ando

Subjects
DNA replication factor CDT1, Ubiquitin, biology, Obligate, Chemistry, Coral, Cell cycle progression, biology.protein, Geminin, mCherry, Fluorescence, Cell biology
Abstract

SummaryWe cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided brighter nuclear labeling for monitoring cell cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively.

Published
2020
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6. Impact of muscle volume loss on acute oral mucositis in patients undergoing concurrent chemoradiotherapy after oral cancer resection.

Author

Yamaguchi, T., Makiguchi, T., Nakamura, H., Yamatsu, Y., Hirai, Y., Shoda, K., Suzuki, K., Kim, M., Kurozumi, S., Motegi, S.I., Shirabe, K., and Yokoo, S.

Subjects
ONCOLOGIC surgery, ORAL cancer, CHEMORADIOTHERAPY, COMPUTED tomography, BONE lengthening (Orthopedics), MUCOSITIS, SKELETAL muscle
Abstract

This study evaluated the association between skeletal muscle mass depletion and severe oral mucositis in patients undergoing concurrent chemoradiotherapy after oral cancer resection. Skeletal muscle mass was evaluated in 60 patients using the skeletal muscle index, which was based on skeletal muscle cross-sectional area (on computed tomography) at the level of the third lumbar vertebra. In accordance with the grading criteria of the Radiation Therapy Oncology Group, patients with a grade ≥3 were defined as having severe oral mucositis. Multivariate logistic regression analysis was used to evaluate independent risk factors for severe oral mucositis. Eleven patients (18.3%) were diagnosed with low skeletal muscle mass. Severe oral mucositis occurred in 17 (28.3%) patients, and the mean skeletal muscle index was 42.8 cm2/m2. A low skeletal muscle mass (hazard ratio 18.1; P = 0.001) and a chemotherapy regimen consisting of 5-fluorouracil and cisplatin (versus cisplatin only) (hazard ratio 5.5; P = 0.015) were independent risk factors for severe oral mucositis. Future prospective studies are warranted to identify effective pre- and perioperative exercises and nutrition programmes to increase low skeletal muscle mass and reduce the incidence of severe oral mucositis in patients undergoing concurrent chemoradiotherapy after oral cancer resection. [ABSTRACT FROM AUTHOR]

Published
2021
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9. Feasibility study for sidewall fluorination of SWCNTs in CF4 plasma.

Author

Shoda, K., Kohno, H., Kobayashi, Y., Takagi, D., and Takeda, S.

Subjects
*CARBON nanotubes, *FLUORINATION, *FEASIBILITY studies, *PLASMA gases, *TRANSMISSION electron microscopy, *X-ray photoelectron spectroscopy, *RAMAN spectroscopy
Abstract

Fluorination of single-wall carbon nanotubes (SWCNTs) was performed by using the CF4 radio-frequency plasma technique. The structural and bonding properties of the plasma-processed SWCNTs were investigated by transmission electron microscopy, x-ray photoelectron spectroscopy, and Raman spectroscopy. The plasma parameters, i.e., self-biased voltage, plasma ion density, and ion dose, substantially affected the structural and bonding properties of plasma-processed SWCNTs. We show that sidewall fluorination of SWCNTs occurs in CF4 plasma at low self-biased voltages. Plasma conditions for the sidewall fluorination of SWCNTs are discussed. [ABSTRACT FROM AUTHOR]

Published
2008
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10. Feasibility study for sidewall fluorination of SWCNTs in C[F.sub.4] plasma

Author

Shoda, K., Kohno, H., Kobayashi, Y., Takagi, D., and Takeda, S.

Subjects
Voltage -- Evaluation, Fluorination -- Analysis, Nanotubes -- Structure, Nanotubes -- Chemical properties, Nanotubes -- Electric properties, Raman spectroscopy -- Usage, Transmission electron microscopes -- Usage, Physics
Abstract

The C[F.sub.4] radio-frequency plasma technique is used for performing the fluorination of single-wall carbon nanotubes (SWCNTs). The studies have shown that sidewall fluorination SWCNTs has occurred in C[F.sub.4] plasma at low self-biased voltages.

Published
2008

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