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345 results on '"Sellergren, Börje"'

4. Rational Design of Highly Selective Sialyllactose‐Imprinted Nanogels.

Author

Contardi, Cecilia, Mavliutova, Liliia, Serra, Massimo, Rubes, Davide, Dorati, Rossella, Vistoli, Giulio, Macorano, Alessio, Sellergren, Börje, and De Lorenzi, Ersilia

Subjects
AFFINITY electrophoresis, BORONIC acid derivatives, CAPILLARY electrophoresis, MOLECULAR imprinting, SYNTHETIC antibodies, IMPRINTED polymers
Abstract

We describe a facile method to prepare water‐compatible molecularly imprinted polymer nanogels (MIP NGs) as synthetic antibodies against target glycans. Three different phenylboronic acid (PBA) derivatives were explored as monomers for the synthesis of MIP NGs targeting either α2,6‐ or α2,3‐sialyllactose, taken as oversimplified models of cancer‐related sT and sTn antigens. Starting from commercially available 3‐acrylamidophenylboronic acid, also its 2‐substituted isomer and the 5‐acrylamido‐2‐hydroxymethyl cyclic PBA monoester derivative were initially evaluated by NMR studies. Then, a small library of MIP NGs imprinted with the α2,6‐linked template was synthesized and tested by mobility shift Affinity Capillary Electrophoresis (msACE), to rapidly assess an affinity ranking. Finally, the best monomer 2‐acrylamido PBA was selected for the synthesis of polymers targeting both sialyllactoses. The resulting MIP NGs display an affinity constant≈106 M−1 and selectivity towards imprinted glycans. This general procedure could be applied to any non‐modified carbohydrate template possessing a reducing end. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Towards molecularly imprinted polymers that respond to and capture phosphorylated tyrosine epitopes using fluorescent bis-urea and bis-imidazolium receptors.

Author

Kislenko, Evgeniia, İncel, Anıl, Gawlitza, Kornelia, Sellergren, Börje, and Rurack, Knut

Abstract

Early detection of cancer is essential for successful treatment and improvement in patient prognosis. Deregulation of post-translational modifications (PTMs) of proteins, especially phosphorylation, is present in many types of cancer. Therefore, the development of materials for the rapid sensing of low abundant phosphorylated peptides in biological samples can be of great therapeutic value. In this work, we have synthesised fluorescent molecularly imprinted polymers (fMIPs) for the detection of the phosphorylated tyrosine epitope of ZAP70, a cancer biomarker. The polymers were grafted as nanometer-thin shells from functionalised submicron-sized silica particles using a reversible addition-fragmentation chain-transfer (RAFT) polymerisation. Employing the combination of fluorescent urea and intrinsically cationic bis-imidazolium receptor cross-linkers, we have developed fluorescent sensory particles, showing an imprinting factor (IF) of 5.0. The imprinted polymer can successfully distinguish between phosphorylated and non-phosphorylated tripeptides, reaching lower micromolar sensitivity in organic solvents and specifically capture unprotected peptide complements in a neutral buffer. Additionally, we have shown the importance of assessing the influence of counterions present in the MIP system on the imprinting process and final material performance. The potential drawbacks of using epitopes with protective groups, which can co-imprint with targeted functionality, are also discussed. [ABSTRACT FROM AUTHOR]

Published
2023
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33. Sialic Acid as a Biomarker Studied in Breast Cancer Cell Lines In Vitro Using Fluorescent Molecularly Imprinted Polymers

Author

El-Schich, Zahra, Zhang, Yuecheng, Göransson, Tommy, Dizeyi, Nishtman, Persson, Jenny L., Johansson, Emil, Caraballo, Remi, Elofsson, Mikael, Shinde, Sudhirkumar, Sellergren, Börje, and Wingren, Anette Gjörloff

Subjects
lcsh:T, Cell- och molekylärbiologi, lcsh:Technology, Sialic acid, lcsh:QC1-999, Analytical Chemistry, lcsh:Chemistry, Breast cancer, breast cancer, Epithelial cell adhesion molecule, lcsh:Biology (General), lcsh:QD1-999, sialic acid, lcsh:TA1-2040, Molecularly imprinted polymers, Analytisk kemi, Nanoparticles, molecularly imprinted polymers, nanoparticles, epithelial cell adhesion molecule, lcsh:Engineering (General). Civil engineering (General), lcsh:QH301-705.5, Cell and Molecular Biology, lcsh:Physics
Abstract

Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation, and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors, correlating with poor prognosis. To overcome the lack of natural SA-receptors, such as antibodies and lectins with high enough specificity and sensitivity, we have used molecularly imprinted polymers (MIPs), or “plastic antibodies”, as nanoprobes. Because high expression of epithelial cell adhesion molecule (EpCAM) in primary tumors is often associated with proliferation and a more aggressive phenotype, the expression of EpCAM and CD44 was initially analyzed. The SA-MIPs were used for the detection of SA on the cell surface of breast cancer cells. Lectins that specifically bind to the a-2,3 SA and a-2,6 SA variants were used for analysis of SA expression, with both flow cytometry and confocal microscopy. Here we show a correlation of EpCAM and SA expression when using the SA-MIPs for detection of SA. We also demonstrate the binding pattern of the SA-MIPs on the breast cancer cell lines using confocal microscopy. Pre-incubation of the SA-MIPs with SA-derivatives as inhibitors could reduce the binding of the SA-MIPs to the tumor cells, indicating the specificity of the SA-MIPs. In conclusion, the SA-MIPs may be a new powerful tool in the diagnostic analysis of breast cancer cells.

Published
2021

34. Highly Efficient Synthesis and Assay of Protein‐Imprinted Nanogels by Using Magnetic Templates

Author

Mahajan, Rashmi, Rouhi, Mona, Shinde, Sudhirkumar, Bedwell, Thomas, Incel, Anil, Mavliutova, Liliia, Piletsky, Sergey, Nicholls, Ian A., and Sellergren, Börje

Subjects
plastic antibodies, Swine, Communication, Acrylic Resins, competitive assays, Nanogels, Enzymes, Immobilized, Communications, Pepsin A, Polymerization, Molecular Imprinting, Molecularly Imprinted Polymers, nanogel synthesis, Animals, Cattle, Trypsin, Magnetite Nanoparticles, Amination
Abstract

We report an approach integrating the synthesis of protein‐imprinted nanogels (“plastic antibodies”) with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino‐functionalized solgel‐coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high‐dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein‐conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited K d

Published
2018

36. Combinatorial design of a sialic acid imprinted binding site exploring a dual ion receptor approach

Author

Mavliutova, Liliia, Verduci, Elena, and Sellergren, Börje

Subjects
mlocular imprinting, crown ether, Biomedicinsk laboratorievetenskap/teknologi, ion par recognition, carbohydrate receptor, Biomedical Laboratory Science/Technology
Abstract

Aberrant sialic acid expression is one of the key indicators of pathological processes. This acidic saccharide is overexpressed in tumor cells and is a potent biomarker. Development of specific capture tools for various sialylated targets is an important step for early cancer diagnosis. However, sialic acid recognition by synthetic hosts is often complicated due to the competition for the anion binding by their counterions, such as Na+ and K+. Here we report on the design of a sialic acid receptor via simultaneous recognition of both the anion and cation of the target analyte. The polymeric receptor was produced using neutral (thio)urea and crown ether based monomers for simultaneous complexation of sialic acid's carboxylate group and its countercation. Thiourea and urea based functional monomers were tested both in solution by 1H NMR titration and in a polymer matrix system for their ability to complex the sodium salt of sialic acid alone and in the presence of crown ether. Combination of both orthogonally acting monomers resulted in higher affinities for the template in organic solvent media. The imprinted polymers displayed enhanced sialic acid recognition driven to a significant extent by the addition of the macrocyclic cation host. The effect of various counterions and solvent systems on the binding affinities is reported. Binding of K+, Na+ and NH4+ salts of sialic acid exceeded the uptake of bulky lipophilic salts. Polymers imprinted with sialic or glucuronic acids displayed a preference for their corresponding templates and showed a promising enrichment of sialylated peptides from the tryptic digest of glycoprotein bovine fetuin.

Published
2021

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