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It is reported that many infants with CDH have evidence of brain injury on postnatal brain magnetic resonance imaging (MRI). Results HT

Abstract: Damage to residual stand after partial harvesting or thinning may lead to serious economic losses in terms of both timber quality at the final harvest, and tree growth reduction. Logging damages and their effect on tree growth were studied in a long term experiment on Corsican pine in central Italy. Damage frequency, agent (felling, skidding), position (root damage, stem base, between 0.3 and 1m a.g.l., >1m a.g.l.) and severity (light, severe) and tree growth were measured after selective thinning from below and at 10 years after the treatment. In detail, we aimed at: monitoring mechanical damages to trees at the end of thinning and after 10 years; and assessing stand stability, growing stock, ring width and basal area at 10 years after the thinning. The thinning removed about 20% of volume, 38% of trees and 26% of basal area. The basal area decreased from 56m2 ha−1 to 42m2 ha−1 but after 10 years it increased again to 56m2 ha−1. Immediately after thinning, 13.6% of the standing trees was damaged, out of these 36.17% showed severe injuries. Damages to standing trees were mainly due to skidding. Ten years after thinning, the percentage of damaged trees was about 17%, out of which 86.67% showed severe wounds. An increase of damaged trees and of trees with severe wounds was observed suggesting that a deeper knowledge on long-term effect of logging damages is needed. This study did not highlight any effect of logging damage on tree growth. In fact, no difference in ring width was recorded between damaged and undamaged trees. [Copyright &y& Elsevier]

Abstract: Purpose: To review our experience with fetal magnetic resonance imaging (MRI) to evaluate congenital heart disease (CHD). Methods: We performed fetal MRI in 32 fetuses with an echocardiographically assessed CHD. Both direct and indirect signs of CHD were investigated. Direct signs considered were: morpho-volumetric abnormalities of the heart; malrotations; ventricular and atrial septal defects; anomalies of the origin, size and course of the great arteries. Indirect signs considered were: difficulty to recognize a “normal” anatomical structures in the reference projections; increase of the vascular size before a stenosis; hypertrophy of the papillary muscles; cardiomegaly and pericardial effusion. All MRI findings were compared with postnatal or autoptic findings. Results: MRI allowed the CHD to be visualised by direct signs in 17 fetuses, indirect signs in 5 and both direct and indirect signs in 9 fetuses, excluding the prenatal echocardiographic suspect of hypoplastic left heart syndrome in 1 fetus. Postnatal echocardiograms or autoptic findings confirmed a normal heart in 1 fetus and CHD in 31 fetuses including a single cardiac anomaly or syndrome in 19 fetuses, 2 associated cardiac abnormalities in 11 and 3 cardiac anomalies in 1 fetus. However, in 2 fetuses MRI detected a ventricular septal defect successively disclosed by gold standard. Conclusions: MRI is a promising method for further assessment of the cardiovascular pathologies diagnosed by echocardiography, and may be a valuable tool in assessing associated extracardiac anomalies. [Copyright &y& Elsevier]

A new paradigm in human genetics is high frequencies of inter-individual variations in copy numbers of specific genomic DNA segments. Such common copy number variation (CNV) loci often contain genes engaged in host-environment interaction including those involved in immune effector functions. DNA sequences within a CNV locus often share a high degree of identity but beneficial or deleterious polymorphic variants are present among different individuals. Thus, common gene CNVs can contribute, both qualitatively and quantitatively, to a spectrum of phenotypic variants. In this review we describe the phenotypic and genotypic diversities of complement C4 created by copy number variations of RCCX modules (RP-C4-CYP21-TNX) and size dichotomy of C4 genes. A direct outcome of C4 CNV is the generation of two classes of polymorphic proteins, C4A and C4B, with differential chemical reactivities towards peptide or carbohydrate antigens, and a range of C4 plasma protein concentrations (from 15 to 70 mg/dl) among healthy subjects. Deliberate molecular genetic studies enabled development of definitive techniques to determine exact patterns of RCCX modular variations, copy numbers of long and short C4A and C4B genes by Southern blot analyses or by real-time quantitative PCR. It is found that in healthy European Americans, the total C4 gene copy number per diploid genome ranges from 2 to 6: 60.8% of people with four copies of C4 genes, 27.2% with less than four copies, and 12% with more than four copies. Such a distribution is skewed towards the low copy number side in patients with systemic lupus erythematosus (SLE), a prototypic autoimmune disease with complex etiology. In SLE, the frequency of individuals with less than four copies of C4 is significantly increased (42.2%), while the frequency of those with more than four copies is decreased (6%). This decrease in total C4 gene copy number in SLE is due to increases in homozygous and heterozygous deficiencies of C4A but not C4B. Therefore, it is concluded that lower copy number of C4 is a risk factor for and higher gene copy number of C4 is a protective factor against SLE disease susceptibility. Copyright © 2009 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Introducing pioneering products is an important entrepreneurial activity and the lifeblood of small businesses, yet previous literature on pioneering and performance in small firms has been inconclusive. Based on data gathered from entrepreneurs in 51 small computer firms, the study found that commitment (entrepreneurial confidence) and adaptability (corporate entrepreneurship and environmental dynamism) were especially beneficial to pioneers. The other three variables (product championing, marketing emphasis, and technological newness) contributed to performance across all new product introductions but did not have modifying effects on pioneering introductions in particular. [ABSTRACT FROM AUTHOR]

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bstract [ABSTRACT FROM AUTHOR]

Vaccine hesitancy was a serious problem in the United States throughout the COVID-19 pandemic, due in part to the reduction in public trust in science that accompanied the pandemic. Now we are facing a new, similar but more extensive problem: booster hesitancy. Even fewer Americans are current on the mRNA booster. We present the results of an experiment with residents of the US who received all initial doses of the mRNA vaccine but who were not up to date on the booster. Participants read a scientific explanation describing either the safety or the effectiveness of the boosters or nothing in the control group. The explanations significantly increased (compared to the control) participants' perceptions of the safety and effectiveness of the mRNA booster, as well as their willingness to get boosted. Explanations also improved emotions and attitudes toward the booster. Furthermore, although liberals were significantly more willing to get boosted than were conservatives, improvement due to the explanations was similar across political ideology groups. However, when interactions were observed, conservatives increased to a greater degree. Importantly, the explanations increased participants' perception of scientists' expertise and knowledge, as well as participants' trust in scientists and the vaccine technology.

Many warnings issued to members of the public are deterministic in that they do not include event likelihood information. This is true of the current polygon-based tornado warning used by the American National Weather Service, although the likelihood of a tornado varies within the boundaries of the polygon. To test whether adding likelihood information benefits end users, two experimental studies and one in-person interview study were conducted. The experimental studies compared five probabilistic formats, two with color and three with numeric probabilities alone, to the deterministic polygon. In both experiments, probabilistic formats led to better understanding of tornado likelihood and higher trust than the polygon alone, although color-coding led to several misunderstandings. When the polygon boundary was drawn at 10% chance, those using probabilistic formats made fewer correct shelter decisions at low probabilities and more correct shelter decisions at high probabilities compared to those using the deterministic warning, although overall decision quality, operationalized as expected value, did not differ. However, when the polygon boundary was drawn around 30%, participants with probabilistic forecasts had higher expected value. The interview study revealed that, although tornado-experienced individuals would not shelter at 10% chance, they would take intermediate actions, such as information-seeking and sharing. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Vaccine hesitancy in the COVID-19 pandemic remained a problem long after mRNA vaccines became available. This may be due in part to misunderstandings about the vaccines, arising from complexities of the science involved. Two experiments, conducted on unvaccinated Americans at two periods postvaccine rollout in 2021, demonstrated that providing explanations, expressed in everyday language, and correcting known misunderstandings, reduced vaccine hesitancy compared to a no-information control group. Four explanations addressing misunderstandings about mRNA vaccine safety and effectiveness were tested in Experiment 1 ( n = 3,787). Some included expository text while others included refutational text, explicitly stating and refuting the misunderstanding. Vaccine effectiveness statistics were expressed either as text or an icon array. Although all four explanations reduced vaccine hesitancy, the refutational format of those addressing vaccine safety (explaining the mRNA mechanism and mild side effects) was the most effective. These two explanations were retested individually and jointly in Experiment 2 ( n = 1,476) later in the summer of 2021. Again, vaccine hesitancy was significantly reduced by all explanations despite differences in political ideology, trust, and prior attitudes. These results suggest that nontechnical explanations of critical issues in vaccine science can reduce vaccine hesitancy, especially when accompanied by refutational text. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Objective: To evaluate fetal brain development using MRI (magnetic resonance imaging) in CDH (congenital diaphragmatic hernia)., Methods: 52 isolated left CDH and 104 control fetuses were imaged using MRI. Brain morphometry (Biparietal diameter-BPD, brain fronto-occipital diameter-BFOD, third ventricle, posterior ventricles, transcerebellar diameter-TCD, anteroposterior and craniocaudal cerebellar vermis diameter-AP and CC) and cortical structures (bilateral cingulate fissure-CF, insular fissure-IF, insular depth - ID) were compared with controls using Mann-Whitney test., Results: Median gestational age at MRI (p = 0.95)and the median biparietal diameter (p = 0.737) were comparable. Among morphometric parameters, only the brain fronto-occipital diameter was significantly smaller in CDH (p = 0.001) and the third ventricle was significantly greater in CDH (<0.0001). Among cortical structures, the cingulate and insular fissures were significantly deeper in CDH fetuses (p < 0.0001) as the insular depth ID was smaller in CDH (p < 0.03)., Conclusions: CDH fetuses have a smaller fronto-occipital diameter, reduced insular depth, deeper cingulate and insular fissure, and greater third ventricle width as compared to controls. These findings suggest that left CDH may have an impact on fetal brain development with an overall reduction in brain volume., (© 2023 The Authors. Prenatal Diagnosis published by John Wiley & Sons Ltd.)

Therapy-related myeloid neoplasms (t-MN) are a distinct subgroup of myeloid malignancies with a poor prognosis that include cases of therapy-related myelodysplastic syndrome (t-MDS), therapy-related myeloproliferative neoplasms (t-MPN) and therapy-related acute myeloid leukemia (t-AML). Here, we report a series of patients with clinical features consistent with juvenile myelomonocytic leukemia (JMML), an overlap syndrome of MDS and myeloproliferative neoplasms that developed after treatment for another malignancy., (© 2021 Wiley Periodicals LLC.)

Background: A growing number of clinical trials have shown that regulatory T (T reg ) cell transfer may have a favorable effect on the maintenance of self-tolerance and immune homeostasis in different conditions such as graft-versus-host disease (GvHD), solid organ transplantation, type 1 diabetes, and others. In this context, the availability of a robust manufacturing protocol that is able to produce a sufficient number of functional T reg cells represents a fundamental prerequisite for the success of a cell therapy clinical protocol. However, extended workflow guidelines for nonprofit manufacturers are currently lacking. Despite the fact that different successful manufacturing procedures and cell products with excellent safety profiles have been reported from early clinical trials, the selection and expansion protocols for T reg cells vary a lot. The objective of this study was to validate a Good Manufacturing Practice (GMP)-compliant protocol for the production of T reg cells that approaches the whole process with a risk-management methodology, from process design to completion of final product development. High emphasis was given to the description of the quality control (QC) methodologies used for the in-process and release tests (sterility, endotoxin test, mycoplasma, and immunophenotype)., Results: The GMP-compliant protocol defined in this work allows at least 4.11 × 10 9 T reg cells to be obtained with an average purity of 95.75 ± 4.38% and can be used in different clinical settings to exploit T reg cell immunomodulatory function., Conclusions: These results could be of great use for facilities implementing GMP-compliant cell therapy protocols of these cells for different conditions aimed at restoring the T reg cell number and function, which may slow the progression of certain diseases., (© 2021. The Author(s).)

Objectives: To compare ultrasound (US) and magnetic resonance imaging (MRI) in the assessment of mediastinal shift angles (MSAs) in fetuses affected by isolated left congenital diaphragmatic hernia (CDH). The use of MRI-MSA and US-MSA as prognostic factor for postnatal survival in fetal left CDH was also explored., Methods: This was an observational study of 29 fetuses with prenatally diagnosed isolated left CDH, assessed with both US and MRI examinations between January 2015 and December 2018. The US-MSA measurements performed within 2 weeks from the MRI assessment were considered for the analysis. The primary outcome was the postnatal survival rate., Results: No significant difference between US and MRI MSAs was detected ( p  = .419). Among the 29 cases, there were 21 alive infants, for an overall postnatal survival rate of 72.41%. After stratifying for postnatal survival, the best cutoffs with the highest discriminatory power in terms of sensibility and specificity were 42.1° for the US-MSA and 39.1° for the MRI-MSA. The performance of MRI-MSA in predicting postnatal survival was close to that of US-MSA in terms of sensitivity (62.5 versus 50.0%), specificity (80.9 versus 90.5%), positive predictive value (55.6 versus 66.7%), negative predictive value (85.0 versus 82.6%) and accuracy (75.9 versus 79.3%). There was no statistically significant difference between the two modalities ( p  > .05 for all)., Conclusions: MRI and US can be interchangeably used for the assessment of MSA in prenatally diagnosed isolated left CDH. Moreover, MSA measured by both US and MRI was confirmed to be correlated with perinatal outcome in terms of survival.

Iron deficiency anemia is associated with heavy menstrual bleeding (HMB) and, by extension, a bleeding disorder (BD). It is unknown if iron deficiency without anemia is associated with a BD in adolescents. Moreover, the threshold of ferritin associated with fatigue in adolescents with HMB is unclear. In this multicenter study, we enrolled adolescents with HMB without BD. Participants underwent BD and anemia work-up in Young Women's Hematology Clinics and completed the Peds QL™ fatigue scale. BDs were defined as von Willebrand Disease, platelet function defect, clotting factor deficiencies, and hypermobility syndrome. Two hundred and fifty consecutive adolescents were enrolled, of whom 196 met eligibility criteria. Overall, 43% (95% confidence interval: 36%-50%) were diagnosed with BD. A total of 61% (n = 119) had serum ferritin levels < 15 ng/mL, 23.5% (n = 46) had iron deficiency only, and 37% (n = 73) had iron deficiency anemia. Low ferritin or ferritin dichotomized as < 15 or ≥ 15 ng/mL was not associated with BD on univariable analysis (p = .24) or when accounting for age, race, ethnicity, body mass index, and hemoglobin (p = .35). A total of 85% had total fatigue score below the population mean of 80.5, and 52% (n = 102) were > 2 SD (or < 54) below the mean, the cut-off associated with severe fatigue. A ferritin threshold of < 6 ng/mL had a specificity of 79.8% but a sensitivity of 36% for severe fatigue. In conclusion, iron deficiency without anemia is not a predictor of BD in adolescents with HMB in a specialty setting. Severe fatigue, especially sleep fatigue, is prevalent in adolescents with BD. Ferritin of < 6 ng/mL has ~80% specificity for severe fatigue in adolescents with HMB., (© 2021 Wiley Periodicals LLC.)

Critical to limiting the spread of Coronavirus disease 2019 (COVID-19) and future pandemics is compliance with behavioral recommendations such as mask wearing and social distancing. Compliance may depend upon understanding the seriousness of the health consequences and the likelihood they will occur. However, the statistics that speak to these issues in an ongoing pandemic are complex and may be misunderstood. An online experiment with a U.S. sample tested the impact on perceived likelihood, trust, concern, behavioral intentions, and agreement with government response of numeric (mortality/infection percentage by age group) and gist expressions (which age group was smaller [mortality] or roughly equivalent [infected]). While the differences in risk perception and willingness to engage in activities between younger and older participants were small, "gist infection and mortality" increased willingness to wear a mask among younger participants. Government restrictions (e.g., social distancing) impacted willingness to engage is risk-reduction and risk-seeking activities. The biggest differences were due to political ideology. Although conservatives perceived similar levels of risk as did liberals, they were much less willing to engage in protective behaviors and support government policies. However, conservatives were affected by some risk communication formats and restrictions suggesting that future work should be aimed at this issue. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Congenital diaphragmatic hernia (CDH) is a severe pediatric disorder with herniation of abdominal viscera into the thoracic cavity. Since neurodevelopmental impairment constitutes a common outcome, we performed morphometric magnetic resonance imaging (MRI) analysis on CDH infants to investigate cortical parameters such as cortical thickness (CT) and local gyrification index (LGI). By assessing CT and LGI distributions and their correlations with variables which might have an impact on oxygen delivery (total lung volume, TLV), we aimed to detect how altered perfusion affects cortical development in CDH. A group of CDH patients received both prenatal (i.e., fetal stage) and postnatal MRI. From postnatal high-resolution T2-weighted images, mean CT and LGI distributions of 16 CDH were computed and statistically compared to those of 13 controls. Moreover, TLV measures obtained from fetal MRI were further correlated to LGI. Compared to controls, CDH infants exhibited areas of hypogiria within bilateral fronto-temporo-parietal labels, while no differences were found for CT. LGI significantly correlated with TLV within bilateral temporal lobes and left frontal lobe, involving language- and auditory-related brain areas. Although the causes of neurodevelopmental impairment in CDH are still unclear, our results may suggest their link with altered cortical maturation and possible impaired oxygen perfusion.