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2. Continuous-flow analysis for glucose, triglycerides, and ATP with immobilized enzymes in tubular form.

Author

León LP, Sansur M, Snyder LR, and Horvath C

Subjects
Autoanalysis, Glucose Oxidase, Glucosephosphate Dehydrogenase, Glycerol Kinase, Hexokinase, Horseradish Peroxidase, Humans, Hydrogen-Ion Concentration, Kinetics, Lipase, Methods, Adenosine Triphosphate analysis, Enzymes, Immobilized, Glucose analysis, Triglycerides analysis
Abstract

Small-bore ("Autozyme") tubes with immobilized enzymes at the inner wall have been developed and studied for application in the Technicon "SMAC" high-speed continuous-flow biochemical analyzer. Tubes coated with glucose oxidase (D-glucose:oxygen oxidoreductase, EC 1.1.3.4) have been prepared for the assay of glucose, with colorimetric assay of the hydrogen peroxide produced; tubes coated with glycerol kinase (ATP:glycerol phosphotransferase, EC 2.7.1.30) for the enzymatic assay of triglycerides; tubes coated with hexokinase (ATP:D-hexose-6-phosphotransferase, EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NAD+ oxidoreductase EC 1.1.1.49) for the measurement of ATP, an intermediate product in assays for creatine kinase. With use of 10-15 cm lengths of Autozyme tube and SMAC hydraulics (150 samples per hour), assay sensitivity and carryover were similar to values for the corresponding free-enzyme methods. These immobilized enzymes were sufficiently stable for one to eight weeks of continuous use before replacemnt. We conclude that suitable bound-enzyme tubes can replace either single or multiple free-enzyme reagents in many continuous-flow assays at high sampling rates.

Published
1977

4. The rate of incorporation of amino acids into brain proteins during infusion in the rat.

Author

Seta K, Sansur M, and Lajtha A

Subjects
Amino Acids administration & dosage, Amino Acids metabolism, Animals, Arginine metabolism, Autoanalysis, Blood-Brain Barrier, Brain Chemistry, Carbon Isotopes, Chromatography, Ion Exchange, Chromatography, Paper, Electrophoresis, Paper, Glutamates metabolism, Glycine metabolism, Half-Life, Hematocrit, Injections, Intravenous, Leucine metabolism, Lysine metabolism, Rats, Time Factors, Tyrosine metabolism, Valine metabolism, Amino Acids blood, Brain metabolism, Nerve Tissue Proteins biosynthesis
Published
1973
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7. Effect of drugs and insecticides on the anesthetic action of steroids.

Author

Kuntzman R, Sansur M, and Conney AH

Subjects
Animals, Liver cytology, Microsomes enzymology, Rats, Androsterone pharmacology, Anesthetics pharmacology, Chlordan pharmacology, DDT pharmacology, Desoxycorticosterone pharmacology, Histamine H1 Antagonists pharmacology, Phenobarbital pharmacology, Phenylbutazone pharmacology, Progesterone pharmacology
Published
1965
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8. DRUG INTERACTIONS IN MAN. I. LOWERING EFFECT OF PHENOBARBITAL ON PLASMA LEVELS OF BISHYDROXYCOUMARIN (DICUMAROL) AND DIPHENYLHYDANTOIN (DILANTIN).

Author

CUCINELL SA, CONNEY AH, SANSUR M, and BURNS JJ

Subjects
Animals, Dogs, Humans, Male, Rats, Antipyrine, Biomedical Research, Blood Coagulation Tests, Chlordan, Dicumarol, Drug Incompatibility, Drug Interactions, Drug Therapy, Liver enzymology, Metabolism, Pharmacology, Phenobarbital, Phenytoin, Toxicology
Published
1965
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9. Clinical implications of lactic dehydrogenase activity in sputum.

Author

Levine I, Flief A, Sansur M, and Wroblewski F

Subjects
Aged, Anti-Bacterial Agents therapeutic use, Bronchial Diseases enzymology, Bronchiectasis diagnosis, Electrophoresis, Expectorants, Female, Humans, Intubation, Intratracheal, Isoenzymes, Methods, Middle Aged, Pneumonia diagnosis, Sodium Chloride, Solutions, Time Factors, L-Lactate Dehydrogenase analysis, Sputum analysis
Published
1969

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